Are there any completely sterile organs or tissues in the human body? Is there any sacred place?

The human microbiome comprises an ample set of organisms that inhabit and interact within the human body, contributing both positively and negatively to our health. In recent years, several research groups have described the presence of microorganisms in organs or tissues traditionally considered as ‘sterile’ under healthy and pathological conditions. In this sense, microorganisms have been detected in several types of cancer, including those in ‘sterile’ organs. But how can the presence of microorganisms be detected? In most studies, 16S and internal transcribed spacer (ITS) ribosomal DNA (rDNA) sequencing has led to the identification of prokaryotes and fungi. However, a major limitation of this technique is that it cannot distinguish between living and dead organisms. RNA-based methods have been proposed to overcome this limitation, as the shorter half-life of the RNA would identify only the transcriptionally active microorganisms, although perhaps not all the viable ones. In this sense, metaproteomic techniques or the search for molecular metabolic signatures could be interesting alternatives for the identification of living microorganisms. In summary, new technological advances are challenging the notion of ‘sterile’ organs in our body. However, to date, evidence for a structured living microbiome in most of these organs is scarce or non-existent. The implementation of new technological approaches will be necessary to fully understand the importance of the microbiome in these organs, which could pave the way for the development of a wide range of new therapeutic strategies

Psychological distress in health sciences college students and its relationship with academic engagement

P. 715-722Objective: To determine the prevalence of psychological distress and its relationship with academic engagement (absorption, dedication and vigor), sex and degree among students from four public universities. Method: A non-experimental, comparative correlational, quantitative investigation without intervention. Study population: 1840 nursing and physical therapy students. The data collection tool used was a questionnaire. Results: A 32.2% prevalence of psychological distress was found in the subjects; a correlation between vigor and psychological distress was found for all of the subjects and also for women. High absorption and dedication scores and low psychological distress scores predicted higher vigor scores. Conclusion: The risk of psychological distress is high, especially for women. Women seem to have a higher level of psychological distress than men. Vigor, energy and mental resilience positively influence psychological distress and can be a vehicle for better results during the learning and studying process.S

Need to introduce teaching of safe and protected teacher training techniques: SAFE FALL

El objeto de este trabajo es identificar si el alumnado que cursa el Grado Educación Primaria de la Facultad de Ciencias de la Educación de la Universidad de Sevilla conoce el riesgo que supone las caídas en la infancia, qué tipo de formación tiene al respecto y si considera importante o no una formación específica sobre la enseñanza de técnicas seguras y protegidas de caer. Para ello se ha diseñado y pasado el cuestionario ad hoc denominado “Cuestionario INFOSECA” a 125 alumnos. Una vez analizados los datos se han obtenido como principales resultados que: el alumnado en su formación inicial como futuro docente, no es consciente de las posibles consecuencias que las caídas pueden producir a los menores. El 88% señala no tener ninguna formación específica en este tipo de contenido. Se concluye que es necesario y primordial introducir en la formación inicial de los futuros docentes de educación primaria, sobre todo a los responsables de las clases de Educación física, un programa de intervención activa para intentar minimizar las consecuencias de los accidentes en escolares mediante la enseñanza de técnicas seguras y protegidas de caer, dando respuesta al problema actual que se nos plantea desde diferentes organismos públicos como la Organización Mundial de la Salud (OMS).The purpose of this paper is to identify if the students who attend the Primary Education Degree of the Faculty of Education of the University of Seville know the risk of falls in childhood, what kind of training has it and if Important or not a specific training on the teaching of safe techniques and protected from falling. For this purpose, the ad hoc questionnaire called "INFOSECA Questionnaire" has been designed and passed to 125 students. Once analyzed the data have been obtained as main results that: the students in their initial formation as future teacher, is not aware of the possible consequences that the falls can produce to the minors. 88% state that they have no specific training in this type of content. It is concluded that it is necessary and essential to introduce in the initial training of future primary education teachers, especially those in charge of Physical Education classes, an active intervention program to try to minimize the consequences of accidents in students through teaching Of safe and protected techniques to fall, responding to the current problem that we are facing from different public organisms such as the World Health Organization (WHO)

Deciphering intrafamilial phenotypic variability by exome sequencing in a Bardet–Biedl family

Bardet–Biedl syndrome (BBS) is a model ciliopathy characterized by a wide range of clinical variability. The heterogeneity of this condition is reflected in the number of underlying gene defects and the epistatic interactions between the proteins encoded. BBS is generally inherited in an autosomal recessive trait. However, in some families, mutations across different loci interact to modulate the expressivity of the phenotype. In order to investigate the magnitude of epistasis in one BBS family with remarkable intrafamilial phenotypic variability, we designed an exome sequencing–based approach using SOLID 5500xl platform. This strategy allowed the reliable detection of the primary causal mutations in our family consisting of two novel compound heterozygous mutations in McKusick–Kaufman syndrome (MKKS) gene (p.D90G and p.V396F). Additionally, exome sequencing enabled the detection of one novel heterozygous NPHP4 variant which is predicted to activate a cryptic acceptor splice site and is only present in the most severely affected patient. Here, we provide an exome sequencing analysis of a BBS family and show the potential utility of this tool, in combination with network analysis, to detect disease-causing mutations and second-site modifiers. Our data demonstrate how next-generation sequencing (NGS) can facilitate the dissection of epistatic phenomena, and shed light on the genetic basis of phenotypic variability

Sistema de indicadores y mapa de resultados esperados del proyecto RESISOR

págs. 39-59.Capítulo incluido en el libro: Informe de Evaluación del Proyecto RESISOR "Regional Single Social Record". M.ª Dolores Muñoz de Dios y José Ignacio García Pérez (autores). Sevilla: Universidad Internacional de Andalucía, 2019. ISBN 978-84-7993-344-9. Enlace: http://hdl.handle.net/10334/3938. En este capítulo se muestra el resultado del trabajo realizado por el grupo de personas expertas en la elaboración del Sistema de Indicadores y Mapa de Resultados del Proyecto RESISOR. Incluye el Marco lógico de Indicadores como propuesta metodológica establecida así como el sistema de indicadores según los grupos de trabajo establecidos para la realización del proyecto: Gestión y Coordinación, Definición de la HSUE, Desarrollo de la HSUE, Implementación de la HSUE,8 Evaluación y Comunicación. También se incorpora tanto el marco lógico de indicadores RESISOR como el mapa de resultados esperados del proyecto

Breaking the Mucin Barrier: A New Affinity Chromatography-Mass Spectrometry Approach to Unveil Potential Cell Markers and Pathways Altered in Pseudomyxoma Peritonei

Background Pseudomyxoma peritonei (PMP) is a rare peritoneal mucinous carcinomatosis with largely unknown underlying molecular mechanisms. Cytoreductive surgery combined with hyperthermic intraperitoneal chemotherapy is the only therapeutic option; however, despite its use, recurrence with a fatal outcome is common. The lack of molecular characterisation of PMP and other mucinous tumours is mainly due to the physicochemical properties of mucin. Results This manuscript describes the first protocol capable of breaking the mucin barrier and isolating proteins from mucinous tumours. Briefly, mucinous tumour samples were homogenised and subjected to liquid chromatography using two specific columns to reduce mainly glycoproteins, albumins and immunoglobulin G. The protein fractions were then subjected to mass spectrometry analysis and the proteomic profile obtained was analysed using various bioinformatic tools. Thus, we present here the first proteome analysed in PMP and identified a distinct mucin isoform profile in soft compared to hard mucin tumour tissues as well as key biological processes/pathways altered in mucinous tumours. Importantly, this protocol also allowed us to identify MUC13 as a potential tumour cell marker in PMP. Conclusions In sum, our results demonstrate that this protein isolation protocol from mucin will have a high impact, allowing the oncology research community to more rapidly advance in the knowledge of PMP and other mucinous neoplasms, as well as develop new and effective therapeutic strategies

Splicing Machinery is Dysregulated in Pituitary Neuroendocrine Tumors and is Associated with Aggressiveness Features

Pituitary neuroendocrine tumors (PitNETs) constitute approximately 15% of all brain tumors, and most have a sporadic origin. Recent studies suggest that altered alternative splicing and, consequently, appearance of aberrant splicing variants, is a common feature of most tumor pathologies. Moreover, spliceosome is considered an attractive therapeutic target in tumor pathologies, and the inhibition of SF3B1 (e.g., using pladienolide-B) has been shown to exert antitumor effects. Therefore, we aimed to analyze the expression levels of selected splicing-machinery components in 261 PitNETs (somatotropinomas/non-functioning PitNETS/corticotropinomas/prolactinomas) and evaluated the direct effects of pladienolide-B in cell proliferation/viability/hormone secretion in human PitNETs cell cultures and pituitary cell lines (AtT-20/GH3). Results revealed a severe dysregulation of splicing-machinery components in all the PitNET subtypes compared to normal pituitaries and a unique fingerprint of splicing-machinery components that accurately discriminate between normal and tumor tissue in each PitNET subtype. Moreover, expression of specific components was associated with key clinical parameters. Interestingly, certain components were commonly dysregulated throughout all PitNET subtypes. Finally, pladienolide-B reduced cell proliferation/viability/hormone secretion in PitNET cell cultures and cell lines. Altogether, our data demonstrate a drastic dysregulation of the splicing-machinery in PitNETs that might be associated to their tumorigenesis, paving the way to explore the use of specific splicing-machinery components as novel diagnostic/prognostic and therapeutic targets in PitNETs

re-habitar El Carmen : Un proyecto sobre patrimonio contemporáneo

El proyecto _re-HABITAR suponía para el propio proceder de la institución un avance más allá del reconocimiento, registro, inventario o protección patrimonial de la arquitectura del siglo XX y del Movimiento Moderno para posicionarse en la acción preventiva y conservativa de ese legado contemporáneo. Para ello, la praxis patrimonial se aferraba a un modelo: el de la vivienda social en España en la segunda mitad del siglo XX; a un caso concreto: el de la barriada de Nuestra Señora del Carmen (Recasens Méndez-Queipo de Llano, 1958); y a un requisito fundamental: analizar un objeto vivo y en uso, aún con la presencia de quienes lo vivieron y usaron desde su origen

La trayectoria urbana de Cartagena de Indias hasta 1586

Este trabajo forma parte de un proyecto planteado por la doctora Carmen Borrego Plá y secundado por el doctor Sigfrido Vázquez Cienfuegos, con la intención de analizar el desarrollo urbano de la ciudad de Cartagena de Indias durante la época colonial, poniendo en relación este aspecto con aquellos otros derivados del componente humano que pobló dicha ciudad. Uno de los objetivos fundamentales del proyecto ha sido la reconstrucción planimétrica de Cartagena de Indias en el siglo XVI, por lo que se ha contado con la inestimable colaboración del ingeniero técnico topógrafo Francisco Muriel Parejo.Peer reviewe