A List of birds seen in Southern Tanzania

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The Paradise Whydah and the Broad-tailed paradise Whydah

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The effects of reinforcement interval on the acquisition of paired-associate responses

Effects of reinforcement interval on acquisition of paired-associate response

Analyses of tissue culture adaptation of human Herpesvirus-6A by whole genome deep sequencing redefines the reference sequence and identifies virus entry complex changes

Tissue-culture adaptation of viruses can modulate infection. Laboratory passage and bacterial artificial chromosome (BAC)mid cloning of human cytomegalovirus, HCMV, resulted in genomic deletions and rearrangements altering genes encoding the virus entry complex, which affected cellular tropism, virulence, and vaccine development. Here, we analyse these effects on the reference genome for related betaherpesviruses, Roseolovirus, human herpesvirus 6A (HHV-6A) strain U1102. This virus is also naturally “cloned” by germline subtelomeric chromosomal-integration in approximately 1% of human populations, and accurate references are key to understanding pathological relationships between exogenous and endogenous virus. Using whole genome next-generation deep-sequencing Illumina-based methods, we compared the original isolate to tissue-culture passaged and the BACmid-cloned virus. This re-defined the reference genome showing 32 corrections and 5 polymorphisms. Furthermore, minor variant analyses of passaged and BACmid virus identified emerging populations of a further 32 single nucleotide polymorphisms (SNPs) in 10 loci, half non-synonymous indicating cell-culture selection. Analyses of the BAC-virus genome showed deletion of the BAC cassette via loxP recombination removing green fluorescent protein (GFP)-based selection. As shown for HCMV culture effects, select HHV-6A SNPs mapped to genes encoding mediators of virus cellular entry, including virus envelope glycoprotein genes gB and the gH/gL complex. Comparative models suggest stabilisation of the post-fusion conformation. These SNPs are essential to consider in vaccine-design, antimicrobial-resistance, and pathogenesis

Relative age affects marathon performance in male and female athletes

Marathon runners are ranked in 5-year age groups. However the extent to which 5-year groupings facilitates equitable competition has not been evaluated. The aim of this study was to evaluate the effect of relative age in male and female marathon running. Marathon finishing times for the top ten male (aged 20-69 years) and female athletes (aged 20-64 years) were obtained from the 2013 New York and Chicago marathons. Intra-class and inter-class validity were evaluated by comparing performances within (intra-class) and between (inter-class) the 5-year age groups. Results showed intra-class effects in all male age groups over 50 years, in all female age groups over 40 years, and in male and female 20-24 age groups (p < 0.05). Inter-class differences existed between the 20-24 and 25-29 age groups in both males and females, between all male age groups over 50 years, and between all female age groups over 40 years (p < 0.05). This study provided the first evaluation of the effects of relative age in male and female marathon running. The results provide preliminary but compelling evidence that the relatively older male athletes in age groups over 50 years and the relatively older females in age groups over 40 years are competitively disadvantaged compared to the younger athletes in these age groups

LPP3 mediates self-generation of chemotactic LPA gradients by melanoma cells

Melanoma cells steer out of tumours using self-generated lysophosphatidic acid (LPA) gradients. The cells break down LPA, which is present at high levels around the tumours, creating a dynamic gradient that is low in the tumour and high outside. They then also migrate up this gradient, creating a complex and evolving outward chemotactic stimulus. Here we introduce a new assay for self-generated chemotaxis, and show that raising LPA levels causes a delay in migration rather than loss of chemotactic efficiency. Knockdown of the lipid phosphatase LPP3 - but not its homologues LPP1 or LPP2 - diminishes the cell's ability to break down LPA. This is specific for chemotactically active LPAs, such as the 18:1 and 20:4 species. Inhibition of autotaxin-mediated LPA production does not diminish outward chemotaxis, but loss of LPP3-mediated LPA breakdown blocks it. Similarly, in both 2D and 3D invasion assays, knockdown of LPP3 diminishes melanoma cells' ability to invade. Our results demonstrate that LPP3 is the key enzyme in melanoma cells' breakdown of LPA, and confirm the importance of attractant breakdown in LPA-mediated cell steering

High-resolution FUSE and HST ultraviolet spectroscopy of the white dwarf central star of Sh 2-216

LS V +4621 is the DAO-type central star of the planetary nebula Sh 2-216. We perform a comprehensive spectral analysis of high-resolution, high-S/N ultraviolet observations obtained with FUSE and STIS aboard the HST as well as the optical spectrum of LS V +4621 by means of state-of-the-art NLTE model-atmosphere techniques in order to compare its photospheric properties to theoretical predictions from stellar evolution theory as well as from diffusion calculations. From the N IV - NV, O IV - O VI, Si IV - Si V, and Fe V - Fe VII ionization equilibria, we determined an effective temperature of 95 +/- 2 kK with high precision. The surface gravity is log g = 6.9 +/- 0.2. An unexplained discrepancy appears between the spectroscopic distance d = 224 +46/-58 pc and the parallax distance d = 129 +6/-5 pc of LS V +4621. For the first time, we have identified Mg IV and Ar VI absorption lines in the spectrum of a hydrogen-rich central star and determined the Mg and Ar abundances as well as the individual abundances of iron-group elements (Cr, Mn, Fe, Co, and Ni). With the realistic treatment of metal opacities up to the iron group in the model-atmosphere calculations, the so-called Balmer-line problem (found in models that neglect metal-line blanketing) vanishes. Spectral analysis by means of NLTE model atmospheres has presently arrived at a high level of sophistication, which is now hampered largely by the lack of reliable atomic data and accurate line-broadening tables. Strong efforts should be made to improve upon this situation.Comment: 13 pages, 20 figure

The Halos of Planetary Nebulae in the Mid-Infrared: Evidence for Interaction with the Interstellar Medium

The motion of planetary nebulae (PNe) through the interstellar medium (ISM) is thought to lead to a variety of observational consequences, including the formation of bright rims; deformation and fragmentation of the shells; and a shift of the central stars away from the geometric centres of the envelopes. These and other characteristics have been noted through imaging in the visual wavelength regime. We report further observations of such shells taken in the mid-infrared (MIR), acquired through programs of IRAC imaging undertaken using the Spitzer Space Telescope (SST). NGC 2440 and NGC 6629 are shown to possess likely interacting halos, together with ram-pressure stripped material to one side of their shells. Similarly, the outer halos of NGC 3242 and NGC 6772 appear to have been fragmented through Rayleigh-Taylor (RT) instabilities, leading to a possible flow of ISM material towards the inner portions of their envelopes. If this interpretation is correct, then it would suggest that NGC 3242 is moving towards the NE; a suggestion which is also supported through the presence of a 60 microns tail extending in the opposite direction, and curved bands of H-alpha emission in the direction of motion - components which may arise through RT instabilities in the magnetized ISM.Comment: 14 pages, 9 figures, Accepted for publication in MNRAS. 42 pages in arXi

Distinct cell shapes determine accurate chemotaxis

Biological and Soft Matter Physic