Loggerhead Sea Turtle (Caretta Caretta) Nesting on a Georgia Barrier Island: Effects of Nest Relocation

The purpose of this study was to determine the effects of nest relocation on nest parameters and embryonic development. The nesting biology of the loggerhead sea turtle (Caretta caretta) was studied on Blackbeard Island in 2005 and 2006, during the nesting season. Research nests were randomly assigned one of two treatments (in-situ or relocated). In-situ nests (n=35) were left in the original location, while relocated nests (n=34) were moved above the spring high-tide line and into areas that were considered to be of favorable nesting conditions. Data-loggers were placed in the center of nests to record the temperature during the incubation duration. Incubation durations, nest temperatures, hatch success, and hatchling straight carapace length were compared for all research nests. The observed nests showed similar nest parameters and embryonic development regardless of nest treatment. Differences in nest parameters and embryonic development seemed to be driven by abiotic conditions of the nesting site. This study shows that nest relocation can be used to alleviate nests of extreme abiotic conditions to increase hatch success, without altering embryonic development

Preservice Teacher Preparation in International Contexts: A Case-Study Examination of the International Student Teacher Programs

This article examines the teacher preparation experiences of preservice teachers in six international contexts: China, Fiji, Kiribati, Mexico, Samoa, and Tonga. More specifically, it looks at the value-added components in an international teacher education program, with an emphasis on effective teaching and employability. Theoretically the study is based on Straus and Corbin’s (1998a) substantive grounded theory and Patton’s (1997) Theory of Action Framework. Verbal and non-verbal forms of feedback were identified as essential aspects of the international preservice training experience. Cultural diversity, teaching English as a second language, collaboration, and exposure to a different educational system were identified among several components as advantages to individuals who conduct their preservice teacher training in international settings.</jats:p

Dynamic energy system modeling using hybrid physics-based and machine learning encoder–decoder models

Three model configurations are presented for multi-step time series predictions of the heat absorbed by the water and steam in a thermal power plant. The models predict over horizons of 2, 4, and 6 steps into the future, where each step is a 5-minute increment. The evaluated models are a pure machine learning model, a novel hybrid machine learning and physics-based model, and the hybrid model with an incomplete dataset. The hybrid model deconstructs the machine learning into individual boiler heat absorption units: economizer, water wall, superheater, and reheater. Each configuration uses a gated recurrent unit (GRU) or a GRU-based encoder–decoder as the deep learning architecture. Mean squared error is used to evaluate the models compared to target values. The encoder–decoder architecture is over 11% more accurate than the GRU only models. The hybrid model with the incomplete dataset highlights the importance of the manipulated variables to the system. The hybrid model, compared to the pure machine learning model, is over 10% more accurate on average over 20 iterations of each model. Automatic differentiation is applied to the hybrid model to perform a local sensitivity analysis to identify the most impactful of the 72 manipulated variables on the heat absorbed in the boiler. The models and sensitivity analyses are used in a discussion about optimizing the thermal power plant

Nutrition Able: A Lecture-based Intervention to Promote Nutritional Literacy About Protein Intake in Women

Sport performance is influenced by sport-specific requirements for body composition. One major predictor of body composition is protein intake. Higher protein intake tends to cause leaner body composition relative to lower protein intake, especially during caloric deficit. In Bexar County, ~72% of the adult population is overweight/obese and 15.5% are diabetic. This suggests that high protein diet could be a high leverage option in the San Antonio population for optimizing body composition for sport performance. Furthermore, late life sporting activity and protein intake is a primary method for mitigating sarcopenia, osteoporosis, fractures, and accidental death in post-menopausal women. PURPOSE: This project, called Nutrition Able, aims to teach Bexar County middle school and high school students in food-scarce areas how to read a nutrition label with their health in mind through hands-on, in-person, lectures in Southside and Southwest ISD classrooms, emphasizing the importance of dietary protein. METHODS: Pairs of health professions students give lectures and administer health literacy assessments. Before each lecture, we inform students that they will be taking an anonymous non-graded survey. The survey consists of 7 multiple-choice questions regarding calories, macronutrients, calories per serving, macronutrient content, fiber, protein, and diabetes/obesity prevention. No assistance interpreting the questions is provided. We also collect the following demographic data at the students’ discretion: height, weight, sex, grade, age, and family history of obesity and diabetes (FH). Following the pre-survey, our lecture is given. Afterwards, an identical post-survey is administered. RESULTS: To date, 11 lectures reaching 432 students between 6th and 12th grade have been given. Overall, there were large improvements in pre-/post-survey scores (2.47 vs. 3.31, P\u3c\u3c\u3c.05), with improvements in all seven questions, median (2 vs. 3), and mode (2 vs. 4). For 6 out of the 7 questions on the survey, male and female students had no significant difference in post-survey rates of correct responses. The question which did reach statistical significance was the question about protein’s health benefits. Women scored lower on this question in both pre- (25% vs. 37%, P=.015) and post-surveys (36% vs. 49%, P=.01), with no difference in the improvement in score. CONCLUSION: Nutrition Able is effective at improving health literacy broadly but has room to improve in emphasizing protein’s efficacy as a tool improving for sport performance and body composition in women. Long-standing myths that lifting weights and eating protein will drastically change your appearance often disincentivize women from engaging in important health promoting behaviors like resistance training and eating a high protein diet, putting them at risk for post-menopausal sarcopenia, osteoporosis, and debilitating fractures. Future talks will further emphasize that protein is an important nutritional factor for all demographics, especially women

Comparing the Safety and Efficacy of a Rapid High-Sensitivity Cardiac Troponin I Protocol Between Hospital-Based and Free-Standing Emergency Departments

Study Objectives: Current high sensitivity cardiac troponin I (hs-cTnI) research has been conducted almost exclusively in hospital-based emergency department (HBED) settings and the translation of these protocols into free-standing emergency departments (FSED) has yet to be explored. This study compared the safety and efficacy of applying a rapid-rule out protocol using hs-cTnI for exclusion of acute myocardial infarction (AMI) in HBEDs and FSEDs. Methods: This was a secondary analysis of a randomized trial of patients evaluated for possible AMI in 9 emergency departments (ED) from July 2020 through March 2021. The trial arms included a new 0/1-hour rapid protocol using hs-cTnI versus standard care, which used a 0/3-hour protocol without reporting hs-cTnI values below the 99th percentile. The primary outcome was safe ED discharge, defined as discharge with no death or AMI within 30-days. Analysis included a mixed-effect model adjusting for demographic variables. Results: There was a statistically significant difference in safe discharges from FSEDs when comparing the standard care arm (86.2%) to the rapid rule-out protocol (95.1%). There was a statistically significant reduction in FSED length of stay with application of a rapid rule-out protocol at 3.43 hours vs. 3.97 hours using standard care. The percentage of patients who ruled-out with their initial hs-cTnI (\u3c4 ng/L) at FSEDs (74%) was also significantly larger when compared to HBEDs (54%). Conclusion: Implementation of a hs-cTnI rapid 0/1-hour protocol to evaluate for AMI in FSEDs is feasible and had greater impact on safe ED discharge and length of stay compared to HBEDs

Donation after Cardiac Death Liver Transplantation: Predictors of Outcome

We aimed to identify recipient, donor and transplant risk factors associated with graft failure and patient mortality following donation after cardiac death (DCD) liver transplantation. These estimates were derived from Scientific Registry of Transplant Recipients data from all US liver-only DCD recipients between September 1, 2001 and April 30, 2009 (n = 1567) and Cox regression techniques. Three years post-DCD liver transplant, 64.9% of recipients were alive with functioning grafts, 13.6% required retransplant and 21.6% died. Significant recipient factors predictive of graft failure included: age ≥ 55 years, male sex, African–American race, HCV positivity, metabolic liver disorder, transplant MELD ≥ 35, hospitalization at transplant and the need for life support at transplant (all, p ≤ 0.05). Donor characteristics included age ≥ 50 years and weight >100 kg (all, p ≤ 0.005). Each hour increase in cold ischemia time (CIT) was associated with 6% higher graft failure rate (HR 1.06, p 100 kg and CIT also increased patient mortality (all, p ≤ 0.035). These findings are useful for transplant surgeons creating DCD liver acceptance protocols.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/79115/1/j.1600-6143.2010.03293.x.pd

Vibration-Heating in ADR Kevlar Suspension Systems

The cryogenics group at NASA's Goddard Space Flight Center has a long-standing development and test program for laboratory and space-flight adiabatic demagnetization refrigerators (ADRs). These devices are used to cool components to temperatures as low as 0.05 K. At such low temperatures the ADR systems can provide a few micro-Watts of cooling power, so it is important to minimize the conduction of heat to these cold stages from the surroundings. The cold ADR elements are held in place by thin tensioned strings made of Kevlar, chosen for its high strength and stiffness and low thermal conductivity. During laboratory testing, we have observed that occasional significant additional heat loads on the coldest ADR stages correlate with unusually high vibration levels in the cryostat due to a noisy mechanical cryocooler. We theorized that this heat results from plastic deformation of the Kevlar fibers and frictional interactions among them, driven by the cryostat vibrations. We describe tests and calculations performed in attempt to confirm this source of the heating, and we discuss possible strategies to reduce this effect in future ADR suspension systems

Cas9 gRNA engineering for genome editing, activation and repression

We demonstrate that by altering the length of Cas9-associated guide RNA(gRNA) we were able to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein. We exploited these principles to engineer mammalian synthetic circuits with combined transcriptional regulation and kill functions governed by a single multifunctional Cas9 protein.National Human Genome Research Institute (U.S.) (P50 HG005550)United States. Department of Energy (DE-FG02-02ER63445)Wyss Institute for Biologically Inspired EngineeringUnited States. Army Research Office (DARPA W911NF-11-2-0054)National Science Foundation (U.S.)United States. National Institutes of Health (5R01CA155320-04)United States. National Institutes of Health (P50 GM098792)National Cancer Institute (U.S.) (5T32CA009216-34)Massachusetts Institute of Technology. Department of Biological EngineeringHarvard Medical School. Department of GeneticsDefense Threat Reduction Agency (DTRA) (HDTRA1-14-1-0006

Effect of Bamlanivimab vs Placebo on Incidence of COVID-19 Among Residents and Staff of Skilled Nursing and Assisted Living Facilities: A Randomized Clinical Trial

IMPORTANCE Preventive interventions are needed to protect residents and staff of skilled nursing and assisted living facilities from COVID-19 during outbreaks in their facilities. Bamlanivimab, a neutralizing monoclonal antibody against SARS-CoV-2, may confer rapid protection from SARS-CoV-2 infection and COVID-19. OBJECTIVE To determine the effect of bamlanivimab on the incidence of COVID-19 among residents and staff of skilled nursing and assisted living facilities. DESIGN, SETTING, AND PARTICIPANTS Randomized, double-blind, single-dose, phase 3 trial that enrolled residents and staff of 74 skilled nursing and assisted living facilities in the United States with at least 1 confirmed SARS-CoV-2 index case. A total of 1175 participants enrolled in the study from August 2 to November 20, 2020. Database lock was triggered on January 13, 2021, when all participants reached study day 57. INTERVENTIONS Participants were randomized to receive a single intravenous infusion of bamlanivimab, 4200mg (n = 588), or placebo (n = 587). MAIN OUTCOMES AND MEASURES The primary outcomewas incidence of COVID-19, defined as the detection of SARS-CoV-2 by reverse transcriptase–polymerase chain reaction and mild or worse disease severity within 21 days of detection, within 8 weeks of randomization. Key secondary outcomes included incidence of moderate or worse COVID-19 severity and incidence of SARS-CoV-2 infection. RESULTS The prevention population comprised a total of 966 participants (666 staff and 300 residents) who were negative at baseline for SARS-CoV-2 infection and serology (mean age, 53.0 [range, 18-104] years; 722 [74.7%] women). Bamlanivimab significantly reduced the incidence of COVID-19 in the prevention population compared with placebo (8.5%vs 15.2%; odds ratio, 0.43 [95%CI, 0.28-0.68]; P < .001; absolute risk difference, −6.6 [95%CI, −10.7 to −2.6] percentage points). Five deaths attributed to COVID-19 were reported by day 57; all occurred in the placebo group. Among 1175 participants who received study product (safety population), the rate of participants with adverse events was 20.1% in the bamlanivimab group and 18.9% in the placebo group. The most common adverse events were urinary tract infection (reported by 12 participants [2%] who received bamlanivimab and 14 [2.4%] who received placebo) and hypertension (reported by 7 participants [1.2%] who received bamlanivimab and 10 [1.7%] who received placebo). CONCLUSIONS AND RELEVANCE Among residents and staff in skilled nursing and assisted living facilities, treatment during August-November 2020 with bamlanivimab monotherapy reduced the incidence of COVID-19 infection. Further research is needed to assess preventive efficacy with current patterns of viral strains with combination monoclonal antibody therapy