Diagnosis and Treatment of Pulmonary Disease in Sea Turtles (Caretta caretta)

The aim of this study was to describe the clinical signs, radiographic, endoscopic and CT findings, cytological and microbiological findings and treatments of pulmonary diseases in sea turtles, in order to obtain an accurate diagnosis that avoids unnecessary therapy and antibiotic-resistance phenomena. In total, 14 loggerheads (Caretta caretta), with clinical and/or radiographic findings of pulmonary pathology, were assessed through various combinations of clinical, radiological, CT, endoscopic examination and bronchoalveolar lavage, which recovered fluid for cytologic and microbiologic analysis. In all cases, radiographic examination led to a diagnosis of pulmonary disorders—4 unilateral and 10 bilateral. All bacteria cultured were identified as Gram-negative. Antibiotic resistance was greater than 70% for all beta-lactams tested. In addition, all bacterial strains were 100% resistant to colistin sulfate and tetracycline. Specific antibiotic therapies were formulated for seven sea turtles using Enrofloxacin, and for four sea turtles using ceftazidime. In two turtles, antibiotic therapy was not included due to the presence of antibiotic resistance against all the antibiotics evaluated. In both cases, the coupage technique and environmental management allowed the resolution of the lung disease without antibiotics. All 14 sea turtles were released back into the sea. Radiographic examination must be considered the gold standard for screening sea turtles that show respiratory signs or abnormal buoyancy. Susceptibility testing with antimicrobials allowed appropriate therapy, including the reduction of antibiotic-resistance

Una experiencia de formación de promotoras contra la violencia de género en los barrios de La Plata y Gran La Plata

En este trabajo presentamos unas primeras reflexiones sobre una experiencia pedagógica realizada en el año 2015 en el marco del Proyecto de Extensión Universitaria: Redescubriendo los lazos sociales del Affidamento. Promotoras para el reposicionamiento subjetivo contra la violencia de género con base en redes cooperativo- comunitarias de la Facultad de Humanidades y Ciencias de la Educación de la UNLP. En el mismo confluimos docentes, graduadas, investigadoras y alumnas de las siguientes Facultades: de Humanidades y Ciencias de la Educación, de Psicología, de Ciencias Jurídicas y Sociales, de Periodismo y Comunicación Social, de Ciencias Naturales y Museo.Eje 4: Prácticas socio-comunitarias en la formación y compromiso social de la universidad. Políticas y estrategias de extensión universitaria: reflexiones y debates.Secretaría de Asuntos Académico

En Affidamento: construyendo redes sociales contra la violencia de género

En este trabajo, compartiremos algunas reflexiones sobre nuestra participación en un Proyecto de Extensión Universitaria realizado en el año 2015, titulado: Redescubriendo los lazos sociales del Affidamento. Promotoras para el reposicionamiento subjetivo contra la violencia de género con base en redes cooperativo- comunitaria con continuidad en el año 2016, en la Facultad de Humanidades y Ciencias de la Educación de la UNLP. En el mismo confluimos docentes, graduadas, investigadoras y alumnas de las siguientes Facultades: de Humanidades y Ciencias de la Educación, de Psicología, de Ciencias Jurídicas y Sociales, de Periodismo y Comunicación Social, de Ciencias Naturales y Museo.Mesa 35: ¿Podremos vivir juntos? Ciudadanía, género y culturas urbanas. La perspectiva de género en la producción de conocimiento sociológicoFacultad de Humanidades y Ciencias de la Educació

En Affidamento: construyendo redes sociales contra la violencia de género

En este trabajo, compartiremos algunas reflexiones sobre nuestra participación en un Proyecto de Extensión Universitaria realizado en el año 2015, titulado: Redescubriendo los lazos sociales del Affidamento. Promotoras para el reposicionamiento subjetivo contra la violencia de género con base en redes cooperativo- comunitaria con continuidad en el año 2016, en la Facultad de Humanidades y Ciencias de la Educación de la UNLP. En el mismo confluimos docentes, graduadas, investigadoras y alumnas de las siguientes Facultades: de Humanidades y Ciencias de la Educación, de Psicología, de Ciencias Jurídicas y Sociales, de Periodismo y Comunicación Social, de Ciencias Naturales y Museo.Mesa 35: ¿Podremos vivir juntos? Ciudadanía, género y culturas urbanas. La perspectiva de género en la producción de conocimiento sociológicoFacultad de Humanidades y Ciencias de la Educació

Una propuesta didáctica singular para el abordaje de la temática de las violencias por razones de género en el espacio escolar

El programa del Taller “Aportes para la sensibilización y la intervención sobre la violencia de género en el espacio escolar”, realizado durante el año 20177 como parte de la oferta de formación de la Dirección de Inclusión Educativa perteneciente a la Prosecretaría de Asuntos Académicos de la UNLP a lxs docentes y preceptorxs de los Colegios preuniversitarios, intentó responder a la convocatoria de brindar una formación en la temática de violencias por razones de género, de carácter preventivo, que implicara instancias de intervención situada. La problemática de interés respondía a la necesidad emergente de los equipos docentes de poder ampliar la formación con base en la capacitación de Educación Sexual Integral (ESI), específicamente en la temática de las violencias por razones de género, atendiendo a las relaciones intergeneracionales que se desarrollan en el espacio escolar.Facultad de Humanidades y Ciencias de la Educació

Construyendo affidamento contra la violencia de género: de un nos-otras a nosotras.

En este trabajo compartiremos algunas reflexiones sobre la experiencia de cinco años de trabajo interdisciplinario en el proyecto de extensión universitaria: "Multiplicando affidamento: promotoras comunitarias en acción. Prácticas en red y estrategias situadas frente a la violencia de género" en coparticipación con el Movimiento Justicia y Libertad. Este proyecto se configuró a partir del surgimiento de una demanda específica en el marco de las acciones llevadas adelante por el grupo de género del Movimiento Justicia y Libertad en su búsqueda por tender redes, affidamentar y potenciar el trabajo contra la violencia de género.Fil: Soza Rossi, Paula Viviana. Universidad Nacional de La Plata; ArgentinaFil: Trotta, Lucía. Universidad Nacional de La Plata; ArgentinaFil: Rodríguez Durán, Adriana. Universidad Nacional de La Plata; ArgentinaFil: Duarte, Yamila. Universidad Nacional de La Plata; ArgentinaFil: Giordano, Antonella. Universidad Nacional de La Plata; ArgentinaFil: Muro, María Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Saavedra 15. Centro de Estudios Urbanos y Regionales; ArgentinaFil: Lofeudo, Graciela. Universidad Nacional de La Plata; Argentin

Surface microbiota of Mediterranean loggerhead sea turtles unraveled by 16S and 18S amplicon sequencing

The loggerhead sea turtle is considered a keystone species with a major ecological role in Mediterranean marine environment. As is the case with other wild reptiles, their outer microbiome is rarely studied. Although there are several studies on sea turtle’s macro-epibionts and endo-microbiota, there has been little research on epibiotic microbiota associated with turtle skin and carapace. Therefore we aimed to provide the identification of combined epibiotic eukaryotic, bacterial and archaeal microbiota on Mediterranean loggerhead sea turtles. In this study, we sampled skins and carapaces of 26 loggerheads from the Mediterranean Sea during 2018 and 2019. To investigate the overall microbial diversity and composition, amplicon sequencing of 16S and 18S rRNA genes was performed. We found that the Mediterranean loggerhead sea turtle epibiotic microbiota is a reservoir of a vast variety of microbial species. Microbial communities mostly varied by different locations and seas, while within bacterial communities’ significant difference was observed between sampled body sites (carapace vs. skin). In terms of relative abundance, Proteobacteria and Bacteroidota were the most represented phyla within prokaryotes, while Alveolata and Stramenopiles thrived among eukaryotes. This study, besides providing a first survey of microbial eukaryotes on loggerheads via metabarcoding, identifies fine differences within both bacterial and eukaryotic microbial communities that seem to reflect the host anatomy and habitat. Multi-domain epi-microbiome surveys provide additional layers of information that are complementary with previous morphological studies and enable better understanding of the biology and ecology of these vulnerable marine reptiles

Benchmarking of Whole Exome Sequencing and Ad Hoc Designed Panels for Genetic Testing of Hereditary Cancer

Next generation sequencing panels have been developed for hereditary cancer, although there is some debate about their cost-effectiveness compared to exome sequencing. The performance of two panels is compared to exome sequencing. Twenty-four patients were selected: ten with identified mutations (control set) and fourteen suspicious of hereditary cancer but with no mutation (discovery set). TruSight Cancer (94 genes) and a custom panel (122 genes) were assessed alongside exome sequencing. Eightythree genes were targeted by the two panels and exome sequencing. More than 99% of bases had a read depth of over 30x in the panels, whereas exome sequencing covered 94%. Variant calling with standard settings identified the 10 mutations in the control set, with the exception of MSH6 c.255dupC using TruSight Cancer. In the discovery set, 240 unique non-silent coding and canonic splice-site variants were identified in the panel genes, 7 of them putatively pathogenic (in ATM, BARD1, CHEK2, ERCC3, FANCL, FANCM, MSH2). The three approaches identified a similar number of variants in the shared genes. Exomes were more expensive than panels but provided additional data. In terms of cost and depth, panels are a suitable option for genetic diagnostics, although exomes also identify variants in non-targeted genes

Benchmarking of Whole Exome Sequencing and Ad Hoc Designed Panels for Genetic Testing of Hereditary Cancer

Acknowledgements: We thank all patients who contributed to this study. The work was supported by grants from the Instituto de Salud Carlos III (ISCIII, MINECO) (operating grants: PI13/00285 and RD12/0036/0008 awarded to C.L. and PIE13/00022 and RD12/0036/0031 awarded to G.C.) and confunded by FEDER funds/European Regional Development Fund (ERDF) - a way to Build Europe-"// FONDOS FEDER "una manera de hacer Europa", the Generalitat de Catalunya (Government of Catalonia) (operating grant 2014SGR338, awarded to G.C.) and the Asociación Española Contra el Cáncer (operating grants, 2010 Grupos Estables, awarded to G.C.). J.B. received a Spanish Society of Medical Oncology grant. This activity is sponsored by the ISCIII Ministerio de Economía y Competitividad (PT13/0001/0044).Next generation sequencing panels have been developed for hereditary cancer, although there is some debate about their cost-effectiveness compared to exome sequencing. The performance of two panels is compared to exome sequencing. Twenty-four patients were selected: ten with identified mutations (control set) and fourteen suspicious of hereditary cancer but with no mutation (discovery set). TruSight Cancer (94 genes) and a custom panel (122 genes) were assessed alongside exome sequencing. Eightythree genes were targeted by the two panels and exome sequencing. More than 99% of bases had a read depth of over 30x in the panels, whereas exome sequencing covered 94%. Variant calling with standard settings identified the 10 mutations in the control set, with the exception of MSH6 c.255dupC using TruSight Cancer. In the discovery set, 240 unique non-silent coding and canonic splice-site variants were identified in the panel genes, 7 of them putatively pathogenic (in ATM, BARD1, CHEK2, ERCC3, FANCL, FANCM, MSH2). The three approaches identified a similar number of variants in the shared genes. Exomes were more expensive than panels but provided additional data. In terms of cost and depth, panels are a suitable option for genetic diagnostics, although exomes also identify variants in non-targeted genes

Impact of opioid-free analgesia on pain severity and patient satisfaction after discharge from surgery: multispecialty, prospective cohort study in 25 countries

Background: Balancing opioid stewardship and the need for adequate analgesia following discharge after surgery is challenging. This study aimed to compare the outcomes for patients discharged with opioid versus opioid-free analgesia after common surgical procedures.Methods: This international, multicentre, prospective cohort study collected data from patients undergoing common acute and elective general surgical, urological, gynaecological, and orthopaedic procedures. The primary outcomes were patient-reported time in severe pain measured on a numerical analogue scale from 0 to 100% and patient-reported satisfaction with pain relief during the first week following discharge. Data were collected by in-hospital chart review and patient telephone interview 1 week after discharge.Results: The study recruited 4273 patients from 144 centres in 25 countries; 1311 patients (30.7%) were prescribed opioid analgesia at discharge. Patients reported being in severe pain for 10 (i.q.r. 1-30)% of the first week after discharge and rated satisfaction with analgesia as 90 (i.q.r. 80-100) of 100. After adjustment for confounders, opioid analgesia on discharge was independently associated with increased pain severity (risk ratio 1.52, 95% c.i. 1.31 to 1.76; P < 0.001) and re-presentation to healthcare providers owing to side-effects of medication (OR 2.38, 95% c.i. 1.36 to 4.17; P = 0.004), but not with satisfaction with analgesia (beta coefficient 0.92, 95% c.i. -1.52 to 3.36; P = 0.468) compared with opioid-free analgesia. Although opioid prescribing varied greatly between high-income and low- and middle-income countries, patient-reported outcomes did not.Conclusion: Opioid analgesia prescription on surgical discharge is associated with a higher risk of re-presentation owing to side-effects of medication and increased patient-reported pain, but not with changes in patient-reported satisfaction. Opioid-free discharge analgesia should be adopted routinely