HP1 reshapes nucleosome core to promote phase separation of heterochromatin

Heterochromatin affects genome function at many levels. It enables heritable gene repression, maintains chromosome integrity and provides mechanical rigidity to the nucleus1,2. These diverse functions are proposed to arise in part from compaction of the underlying chromatin2. A major type of heterochromatin contains at its core the complex formed between HP1 proteins and chromatin that is methylated on histone H3, lysine 9 (H3K9me). HP1 is proposed to use oligomerization to compact chromatin into phase-separated condensates3-6. Yet, how HP1-mediated phase separation relates to chromatin compaction remains unclear. Here we show that chromatin compaction by the Schizosaccharomyces pombe HP1 protein Swi6 results in phase-separated liquid condensates. Unexpectedly, we find that Swi6 substantially increases the accessibility and dynamics of buried histone residues within a nucleosome. Restraining these dynamics impairs compaction of chromatin into liquid droplets by Swi6. Our results indicate that Swi6 couples its oligomerization to the phase separation of chromatin by a counterintuitive mechanism, namely the dynamic exposure of buried nucleosomal regions. We propose that such reshaping of the octamer core by Swi6 increases opportunities for multivalent interactions between nucleosomes, thereby promoting phase separation. This mechanism may more generally drive chromatin organization beyond heterochromatin

Study of in-medium ω\omega meson properties in Ap, pA and AA collisions

We propose to investigate the in-medium properties of vector $\omega$ mesons at the normal nuclear density in Ap(pA) collisions and at higher density in AA collisions at the ITEP accelerator facility TWAC. Using of the inverse Ap kinematics will permit us to study the $\omega$ meson production in a wide momentum interval included the not yet explored range of small meson momenta relative to the projectile nuclei where the mass modification effect in nuclear matter is expected to be the strongest. Momentum dependence of the in-medium $\omega$ meson width will be studied in the traditional pA kinematics. We intend to use the electromagnetic calorimeter for reconstruction of the $\omega$ meson invariant mass by detecting photons from the $\omega \to \pi^{0}\gamma \to 3\gamma$ decay. The model calculations and simulations with RQMD generator show feasibility of the proposed experiment. Available now intensity of the ion beams provides a possibility to collect large statistics and make decisive conclusion about the $\omega$ meson properties at density of normal nuclei. At the second stage of the investigation the $\omega$ meson properties will be studied in AA collisions at higher density. Interpretation of these measurements will be based on the results obtained in Ap(pA) interactions. Further investigation of the in-medium properties of light unflavored and charmed mesons can be performed at ITEP and at GSI(FAIR) where higher ion energies will be accessible in near future.Comment: 26 pages, 10 figures, 2 table

The spectral function of the omega meson in nuclear matter from a coupled-channel resonance model

We calculate the spectral function of the omega meson in nuclear matter at zero temperature by means of the low-density theorem. The omega N forward scattering amplitude is calculated within a unitary coupled-channel effective Lagrangian model that has been applied successfully to the combined analysis of pion- and photon-induced reactions. While the peak of the omega spectral distribution is shifted only slightly, we find a considerable broadening of the omega meson due to resonance-hole excitations. For omega mesons at rest with respect to the surrounding nuclear medium, we find an additional width of about 60 MeV at saturation density.Comment: 26 pages, 10 figures, added short discussio

Measurement of the Beam Asymmetry Σ\Sigma in the Forward Direction for pi0 Photoproduction

Photoproduction of neutral pions has been studied with the CBELSA/TAPS detector for photon energies between 0.92 and 1.68~GeV at the electron accelerator ELSA. The beam asymmetry~$\Sigma$ has been extracted for $115^\circ < \theta_{\rm c.m.} < 155^\circ$ of the $\pi^0$~meson and for $\theta_{\rm c.m.} < 60^\circ$. The new beam asymmetry data improve the world database for photon energies above 1.5~GeV and, by covering the very forward region, extend previously published data for the same reaction by our collaboration. The angular dependence of $\Sigma$ shows overall good agreement with the SAID parameterization.Comment: 11 pages, 10 figures, published in Phys. Rev. C, included LEPS data and MAID 2007 predictions for comparison with our dat

Photoproduction of Neutral Pions off Protons

Photoproduction of neutral pions has been studied with the CBELSA/TAPS detector in the reaction $\gamma p\to p\pi^0$ for photon energies between 0.85 and 2.50 GeV. The $\pi^0$ mesons are observed in their dominant neutral decay mode: $\pi^0\to\gamma\gamma$. For the first time, the differential cross sections cover the very forward region, $\theta_{\rm c.m.}<60^\circ$. A partial-wave analysis of these data within the Bonn-Gatchina framework observes the high-mass resonances $G_{17}$(2190), $D_{13}$(2080), and $D_{15}$(2070).Comment: 12 pages, 9 figures, minor modifications to PWA sectio

On the background in the γp→ω(π0γ)p\gamma p \to \omega(\pi^0\gamma) p reaction and mixed event simulation

In this paper we evaluate sources of background for the $\gamma p \to \omega p$, with the $\omega$ detected through its $\pi^0 \gamma$ decay channel, to compare with the experiment carried out at ELSA. We find background from $\gamma p \to \pi^0 \pi^0 p$ followed by decay of a $\pi^0$ into two $\gamma$, recombining one $\pi^0$ and one $\gamma$, and from the $\gamma p \to \pi^0 \eta p$ reaction with subsequent decay of the $\eta$ into two photons. This background accounts for the data at $\pi^0 \gamma$ invariant masses beyond 700 MeV, but strength is missing at lower invariant masses which was attributed to photon misidentification events, which we simulate to get a good reproduction of the experimental background. Once this is done, we perform an event mixing simulation to reproduce the calculated background and we find that the method provides a good description of the background at low $\pi^0 \gamma$ invariant masses but fakes the background at high invariant masses, making background events at low invariant masses, which are due to $\gamma$ misidentification events, responsible for the background at high invariant masses which is due to the $\gamma p \to \pi^0 \pi^0 p$ and $\gamma p \to \pi^0 \eta p$ reactions.Comment: 10 pages, 5 figure

Spectroscopic, Morphological and Mechanistic Investigation of the Solvent.Promoted Aggregation of Porphyrins Modified in meso-positions by Glucosylated steroids

Solvent-driven aggregation of a series of porphyrin derivatives was studied by UV/Vis and circular dichroism spectroscopy. The porphyrins are characterised by the presence in the meso positions of steroidal moieties further conjugated with glucosyl groups. The presence of these groups makes the investigated macrocycles amphiphilic and soluble in aqueous solvent, namely, dimethyl acetamide/ water. Aggregation of the macrocycles is triggered by a change in bulk solvent composition leading to formation of large architectures that express supramolecular chirality, steered by the presence of the stereogenic centres on the periphery of the macrocycles. The aggregation behaviour and chiroptical features of the aggregates are strongly dependent on the number of moieties decorating the periphery of the porphyrin framework. In particular, experimental evidence indicates that the structure of the steroid linker dictates the overall chirality of the supramolecular architectures. Moreover, the porphyrin concentration strongly affects the aggregation mechanism and the CD intensities of the spectra. Notably, AFM investigations reveal strong differences in aggregate morphology that are dependent on the nature of the appended functional groups, and closely in line with the changes in aggregation mechanism. The suprastructures formed at lower concentration show a network of long fibrous structures spanning over tens of micrometres, whereas the aggregates formed at higher concentration have smaller rodshaped structures that can be recognised as the result of coalescence of smaller globular structures. The fully steroid substituted derivative forms globular structures over the whole concentration range explored. Finally, a rationale for the aggregation phenomena was given by semiempirical calculations at the PM6 level

On different lagrangian formalisms for vector resonances within chiral perturbation theory

We study the relation of vector Proca field formalism and antisymmetric tensor field formalism for spin-one resonances in the context of the large N_C inspired chiral resonance Lagrangian systematically up to the order O(p6) and give a transparent prescription for the transition from vector to antisymmetric tensor Lagrangian and vice versa. We also discuss the possibility to describe the spin-one resonances using an alternative "mixed" first order formalism, which includes both types of fields simultaneously, and compare this one with the former two. We also briefly comment on the compatibility of the above lagrangian formalisms with the high-energy constraints for concrete VVP correlator.Comment: 34 pages, 3 figure

Photoproduction of meson pairs: First measurement of the polarization observable I^s

The polarization observable I^s, a feature exclusive to the acoplanar kinematics of multi-meson final states produced via linearly polarized photons, has been measured for the first time. Results for the reaction g p -> p pi0 eta are presented for incoming photon energies between 970 MeV and 1650 MeV along with the beam asymmetry I^c. The comparably large asymmetries demonstrate a high sensitivity of I^s to the dynamics of the reaction. Fits using Bonn-Gatchina partial wave analysis demonstrate that the new polarization observables carry significant information on the contributing partial waves.Comment: 11 pages, 6 figures, v2 to appear in Phys. Lett.

Unification of Residues and Grassmannian Dualities

The conjectured duality relating all-loop leading singularities of n-particle N^(k-2)MHV scattering amplitudes in N=4 SYM to a simple contour integral over the Grassmannian G(k,n) makes all the symmetries of the theory manifest. Every residue is individually Yangian invariant, but does not have a local space-time interpretation--only a special sum over residues gives physical amplitudes. In this paper we show that the sum over residues giving tree amplitudes can be unified into a single algebraic variety, which we explicitly construct for all NMHV and N^2MHV amplitudes. Remarkably, this allows the contour integral to have a "particle interpretation" in the Grassmannian, where higher-point amplitudes can be constructed from lower-point ones by adding one particle at a time, with soft limits manifest. We move on to show that the connected prescription for tree amplitudes in Witten's twistor string theory also admits a Grassmannian particle interpretation, where the integral over the Grassmannian localizes over the Veronese map from G(2,n) to G(k,n). These apparently very different theories are related by a natural deformation with a parameter t that smoothly interpolates between them. For NMHV amplitudes, we use a simple residue theorem to prove t-independence of the result, thus establishing a novel kind of duality between these theories.Comment: 56 pages, 11 figures; v2: typos corrected, minor improvement