Isolation and characterisation of the cyanobacterial chlorophyll F synthase

The D1 protein encoded by the psbA gene forms the reaction centre of photosystem II (PSII) and provides most of the ligands for binding the Mn4CaO5 cluster. Although the protein is conserved among cyanobacteria, five groups of D1 (G0-G4) have been identified, some of which (G0, G1 and G2) appear from sequence comparisons to be non-functional for oxygen evolution. The G1 group of D1, which contains so-called super-rogue versions of D1 (srD1), is expressed in cyanobacteria that synthesise chlorophyll f (Chl f) during far-red light photoacclimation (FaRLiP). Ho et al. (2016) have recently suggested that the srD1 is the chlorophyll f synthase (ChlF) and might form a homodimeric complex in the membrane, but the biochemical evidence is currently lacking. In this thesis I show that heterologous expression of srD1 encoded by the chlF gene of Chroococcidiopsis thermalis PCC 7203 in Synechocystis PCC 6803 leads to the production of Chl f. Two-dimensional (2D) polyacrylamide gel electrophoresis analysis of immuno-purified FLAG-ChlF indicated that ChlF forms a heterodimer with D2 and is incorporated into a novel type of monomeric PSII complex, termed the super-rogue PSII complex. Immunoblotting experiments and mass spectrometry revealed the presence of several low-molecular-mass subunits of PSII and Psb27. 77K fluorescence spectra of the isolated FLAG-ChlF complex showed a peak at 715 nm, consistent with the presence of Chl f. Characterisation of mutants lacking PSII core protein subunits indicated that the presence of CP43, CP47, and D2 was required for the accumulation of Chl f. Analysis of PSII activity in cells showed that the PSII complex containing ChlF was not functional for water oxidation, suggesting that this particular type of complex acts as a Chl f-producing PSII. Characterisation of chimeric D1 mutants containing portions of ChlF indicated that a QD motif in ChlF is vital for the production of Chl f. In addition, the chlF gene (3XFLAG-tagged) was successfully integrated into the Chlamydomonas reinhardtii chloroplast genome. Immunoblotting experiments of thylakoid membranes separated by 2D gel electrophoresis revealed that FLAG-ChlF was present in a large complex that co-migrated with a monomeric PSII fraction. Overall, this work identifies a new type of PSII complex which plays a physiological role in Chl f biosynthesis rather than water splitting.Open Acces

Competition and stability in the credit industry: banking vs. factoring industries

Over the last decade, most credit-industries registered a decline in lending volumes, while factoring industries instead registered a substantial growth in terms of turnover. Surprisingly, only a handful of papers so far investigate factoring companies. Do factoring firms display the same stability levels of banks? Is the competition similar in factoring and banking industries? Is the relationship between competition and stability the same in these industries? Focusing on Italy (one of the largest factoring and banking markets in Europe) and using a unique dataset, we show three main results: factoring companies are (on average) more stable than banks; 2) the stability of factoring companies increase when competition declines (competition-fragility view); 3) the competition-fragility view is weaker in the factoring industry than in the banking industry. Our findings indicate that competition in the Italian credit industry was greater in factoring than in banking

PONTIANAK CHRISTIAN PAGUYUBAN EFFORTS IN MAINTAINING MECHANICAL SOCIAL SOLIDARITY OF MEMBERS

The objective of this research was to know the effort of Paguyuban Kristen Jawi Pontianak maintain collective consciousness, religious ties and social ties. The research used descriptive method and qualitative approach. The techniques of data collection were observation, interview and the study of documentation. The research instruments were the directive of observation, directive of interview, and the documentation tools. This research showed that collective consciousness of the gemeinschaft is already. There is effort done maintain the collective consciousness as for mutually coordinate and worship priority.  This religious tie of the gemeinschaft is good enough. There is effort done maintain the religious tie is get the religous vacation on specific moments that run every year. Social tie of the gemeinschaft was good. There is effort done maintain the social tie as run with instilling tolerance to gemeinschaft members and set the schedule activity and then run that as consistance

Overconfidence and Excessive Trading Behavior: An Experimental Study

The main objective of the research is to examine the excessive trading hypothesis, investors who have higher overconfidence shown by high miscalibration levels will tend to practice aggresive and excessive trading strategy. It is an experimental research which combines both between and within subject design. The participants are undergraduate students who have already taken financial management course but have not yet invest in real capital market. The result of the research shows that high overconfidence investors have higher trading activity than low overconfidence investor. The other result shows that among high overconfidence investors, there is no trading activity differences between pre and post bad news, whereas among low overconfidence investors, the existence of bad news cause trading activity to decrease in the post bad news period. Then, the investment returns of high overconfidence investors is significantly lower than that of the low overconfidence investors

DOES MISPRICING AFFECT INVESTMENT AND CAPITAL STRUCTURE OF INDONESIAN FIRMS?

Stock price movement is not entirely a reflection of its fundamental value because of there are non-fundamental factors such as market sentiment (Keynes, 1936), behavioral biases of investors (Lakonishok et al., 1994), systematic errors when assessing stock (Stein, 1996), asymmetric information (Tobin, 1969) causing the value of stock deviate from its fundamental value (misprice). This condition can affect corporate investment decisions because managers can take advantage of overvalued stock condition as a source of investment funding because the cost of capital becomes cheaper. Conversely, firms avoid selling stocks at undervalued due to high cost of capital. Therefore, the objectives of this research is to examine the effect of mispricing to firms investment behavior and to firms capital structure. We also test the role of the level of financial constraint in the relationship between mispricing and investment. Using panel data regression with data observation for five years, we find that mispricing have positif impact to firms investment level. However, this effect is not diverse whether on a group of firms which have a high level of financial constraint (financially constraint) or those which have a low level of financial constraint (less constraint). Moreover, this research also find that the mispricing can also influence firms in choosing sources of funding which can be seen on their debt to equity ratio (D/E). To check the accuracy of examination, we employ some robustness test and use several control variables. These results are consistent with and can be explained using market timing and catering hypotesis

DETEKSI FITUR CITRA STEREO TERPOLARISASI

Pengolahan citra terpolarisasi membutuhkan penanganan khusus, terlebih jika kita menggunakan citra ini dalam sistem stereo. Citra terpolarisasi yang terbentuk dari instalasi sistem optik pada sistem pencitraan (imaging system) konvensional akan menghasilkan citra yang berkualitas rendah. Pasangan citra yang cenderung ’gelap’ jika diterapkan begitu saja dalam sistem stereo untuk melakukan pencocokan citra (image matching) kiri dan kanan akan menghasilkan kecocokan yang rendah pula

Fintech Studies in Indonesia: A Systematic Literature Review

The advancement of information technology has brought digital transformation on many aspects of human life, including in financial activities. This leads to the emergence of financial technology (fintech) has proved to be one of the most significant phenomena for the last decade. Thus, scientific studies in this field are important for the literature development to provide evidence in the field as well as contribute to the development of fintech innovations, regulations, and practices. The study explores the available research publications in fintech topic in Indonesian literature using systematic literature review method. Based on our analysis, we conclude that the studies in the area of fintech have covered most of the available topics for all of the existing fintech products and potential issues. However, several topics still requires further research as they are considerably less explored. Further, the quality of studies also needs to be improved as there are a lot of studies with quality issues. Finally, the fintech studies in Indonesia can enrich the Indonesian fintech literature with good quality paper publications in reputable publishers.Keywords: financial technology, fintech, Indonesia, systematic literature review

FACEBOOK CONTENTS AND JOB RECRUITMENT: AN EXPERIMENTAL STUDY

Abstract. This paper presents an experimental study exploring the effect of Facebook applicants' content on the recruitment decisions made by job recruiters. Facebook content is divided into professional and non-professional contents. We employ 40 participants in this experimental design. Our results show that the applicants’ Facebook contents significantly affect the recruitment decisions which is indicated by the difference of choice from the first phase to third phase. Qualified applicants are associated with more professional contents on their Facebook accounts