The tested and measured coefficients of the protective materials to keep from harm of the ionizing radiation

У статті викладено результати лабораторних випробувань нових матеріалів, призначених для виготовлення засобів індивідуального захисту для працівників атомних електричних станцій, які обмежують вплив зовнішніх іонізуючих випромінювань. Експериментально встановлено, що запропоновані матеріали мають захисні властивості від α-, β-випромінювань. Визначено коефіцієнти захисту і встановлено їхні параметричні залежності від кількості шарів матеріалу.The article presents the results of laboratory tests of the developed materials, used for the manufacture of permeable protective clothing, which has characteristics to limit the impact of external ionizing radiation. It has been experimentally proved that these materials have the properties to protect from α-, β-ionizing radiation. The coefficients of protection were determined and their parametric dependence on the number of material layers was defined.В статье изложены результаты лабораторных испытаний новых материалов, предназначенных для изготовления средств индивидуальной защиты, которые ограничивают влияние внешних ионизирующих излучений на работников атомных электрических станций. Экспериментально установлено, что предложенные материалы имеют защитные свойства от α-, β-излучений. Определены коэффициенты защиты и установлены их параметрические зависимости от количества слоев материала

What’s new in genitourinary pathology 2023: WHO 5th edition updates for urinary tract, prostate, testis, and penis

The 5th edition WHO Classification of Urinary and Male Genital Tumours (2022) introduced many significant changes relevant to urologic daily practice, mainly to renal tumors which was covered in the What’s New newsletter in September 2022. In this newsletter, we summarize the notable changes to bladder, prostate, testis, and penis based on the 5th edition of the WHO

Agrobiological assessment of productivity and nitrogen fixation of vegetable soybean (edamame) in the conditions of Forest-Steppe of Ukraine

Received: May 25th, 2023 ; Accepted: October 18th, 2023 ; Published: October 30th, 2023 ; Correspondence: [email protected] evaluation of vegetable soybean cultivars (Glycine max var. Shirofumi) according to a complex of economically valuable traits for introduction in the conditions of the Forest-Steppe of Ukraine it was carried out with the aim of selecting the source material and selecting promising breeding forms according to morpho-biological and physiologicalbiochemical characteristics. The research was conducted in the conditions of the educational and production department of Uman National University of Horticulture during 2020–2022, collection cultivars of different ecological and geographical origins were used. The cultivars ‘Sac’ (166.00 g plant-1 ), ‘Vesta’ (139.33 g plant-1 ), ‘Fiskeby V’ (133.33 g plant-1 ), and ‘Fiskeby V-E5’ (146.67 g plant-1 ) possessed a large mass of edamame beans. The maximum yield of edamame beans was formed by plants of the ‘L 380-2-13’ (12.67 t ha-1 ), ‘Vesta’ (12.33 t ha-1 ), ‘Sac’ (13.20 t ha-1 ), ‘Fiskeby V’ (13.97 t ha-1 ), and ‘Fiskeby V-E5’ (14.53 t ha-1 ). Weather conditions during the period of research significantly influenced the yield and biochemical composition of edamame varieties. The yield of edamame beans ranged from 5.40 t ha-1 in 2020 (min) to 22.40 t ha-1 in 2021 (max), and the coefficient of variation by varieties was at the level of 19–41%. This phenomenon is explained by the minimum amount of precipitation in 2020 and the high amount in 2021, which is confirmed by the hydrothermal coefficient during the period of intensive pod growth (VII–VIII) - 2020 - 0.3; 2021 - 1.3 and 1.1 respectively for the month. The protein concentration in Edamame beans was in the range of 27.94–36.29%. A higher protein content relative to the standard was noted in one sample - ‘Karikachi’ - 36.29%. Minimal accumulation of oligosaccharides was noted in ‘Sac’ and ‘Astra’ cultivars, which indicates their suitability for consumption. Promising cultivars were identified by the amount of fixed nitrogen - ‘Sac’ (168.00 ± 4.32 kg ha-1 ), ‘Astra’ (161.67 ± 2.36 kg ha-1 ) and SybNYYSOH 6 (158.19 ± 4.56 kg ha-1 ). The conducted regression analysis showed close relationships (from moderate to functional) between various indicators of the development and productivity of the nodulation apparatus of edamame cultivars. The evaluation of the collection cultivars of vegetable soybeans, their use according to the variability of morphological features and productivity made it possible to single out the ‘Sac’ among the introduced collection cultivars based on a set of valuable traits for use in the selection process to create new cultivars of vegetable soybeans

Emotional burning of preschool teachers: factors of development, diagnostics, prevention of art therapy and self-identification

The article examines the actual aspect of health in the profession of preschool teacher. The authors have shown that the day-to-day psychological work of a caregiver requires the development of effective methods of preventing occupational stress in order to preserve the psychological health of the caregiver’s personality and to find ways to overcome emotional burnout. The factors of the development of teachers’ emotional burnout in modern preschool institutions have been established. The essence of the concepts of «emotional burnout» and «professional burnout» has been clarified. With the help of diagnostic methods of research the indicators of emotional burnout levels, strong-willed self-regulation, manifestations of anxiety, resistance self-esteem to stress were revealed

Designing of rational structure of range of insulating protective clothing on the basis of the principles of transformation

The aim of this work is to develop a range structure of insulating change of clothes with predictable characteristics for the employees of nuclear power plants (NPP). It was applied a systematic approach to solve the assigned tasks, which made it possible to use the principles of transformation through the gradual changes in design and complete of protective clothing. The implementation of the assigned tasks was carried out using the method of structural optimization of construction of insulating protective clothing. The rational structure of range of insulating change of clothes with predictable coefficient of protection, weight and cost was formed on the basis of the principles of transformation by the method of structural optimization. It was obtained the formula for calculation of protection coefficient under constraints on weight, cost, number of material layers (taking into consideration the non-linearity of the objective function), under constraints and discretization of initial information for the packages of protective clothing. Structural and logical matrix for insulating change of protective clothing was formed. There were proposed a structure of insulating change of clothing for NPP employees, structural and technological solutions for the products from the product range. Task-oriented selection of modules and methods of their transformation within the designed object was carried out

Reprogramming of CTLs into natural killer–like cells in celiac disease

Celiac disease is an intestinal inflammatory disorder induced by dietary gluten in genetically susceptible individuals. The mechanisms underlying the massive expansion of interferon γ–producing intraepithelial cytotoxic T lymphocytes (CTLs) and the destruction of the epithelial cells lining the small intestine of celiac patients have remained elusive. We report massive oligoclonal expansions of intraepithelial CTLs that exhibit a profound genetic reprogramming of natural killer (NK) functions. These CTLs aberrantly expressed cytolytic NK lineage receptors, such as NKG2C, NKp44, and NKp46, which associate with adaptor molecules bearing immunoreceptor tyrosine-based activation motifs and induce ZAP-70 phosphorylation, cytokine secretion, and proliferation independently of T cell receptor signaling. This NK transformation of CTLs may underlie both the self-perpetuating, gluten-independent tissue damage and the uncontrolled CTL expansion leading to malignant lymphomas in severe forms of celiac disease. Because similar changes were detected in a subset of CTLs from cytomegalovirus-seropositive patients, we suggest that a stepwise transformation of CTLs into NK-like cells may underlie immunopathology in various chronic infectious and inflammatory diseases

Genomic analysis of estrogen cascade reveals histone variant H2A.Z associated with breast cancer progression

We demonstrate an integrated approach to the study of a transcriptional regulatory cascade involved in the progression of breast cancer and we identify a protein associated with disease progression. Using chromatin immunoprecipitation and genome tiling arrays, whole genome mapping of transcription factor-binding sites was combined with gene expression profiling to identify genes involved in the proliferative response to estrogen (E2). Using RNA interference, selected ERα and c-MYC gene targets were knocked down to identify mediators of E2-stimulated cell proliferation. Tissue microarray screening revealed that high expression of an epigenetic factor, the E2-inducible histone variant H2A.Z, is significantly associated with lymph node metastasis and decreased breast cancer survival. Detection of H2A.Z levels independently increased the prognostic power of biomarkers currently in clinical use. This integrated approach has accelerated the identification of a molecule linked to breast cancer progression, has implications for diagnostic and therapeutic interventions, and can be applied to a wide range of cancers

Analysis of Drosophila segmentation network identifies a JNK pathway factor overexpressed in kidney cancer

We constructed a large-scale functional network model in Drosophila melanogaster built around two key transcription factors involved in the process of embryonic segmentation. Analysis of the model allowed the identification of a new role for the ubiquitin E3 ligase complex factor SPOP. In Drosophila, the gene encoding SPOP is a target of segmentation transcription factors. Drosophila SPOP mediates degradation of the Jun kinase phosphatase Puckered, thereby inducing tumor necrosis factor (TNF)/Eiger-dependent apoptosis. In humans, we found that SPOP plays a conserved role in TNF-mediated JNK signaling and was highly expressed in 99% of clear cell renal cell carcinomas (RCCs), the most prevalent form of kidney cancer. SPOP expression distinguished histological subtypes of RCC and facilitated identification of clear cell RCC as the primary tumor for metastatic lesions

Lactic acid fermentation as a tool to enhance the antioxidant properties of Myrtus communis berries

Background: Myrtle (Myrtus communis L.) is a medicinal and aromatic plant belonging to Myrtaceae family, which is largely diffused in the Mediterranean areas and mainly cultivated in Tunisia and Italy. To the best of our knowledge, no studies have already considered the use of the lactic acid fermentation to enhance the functional features of M. communis. This study aimed at using a selected lactic acid bacterium for increasing the antioxidant features of myrtle berries, with the perspective of producing a functional ingredient, dietary supplement or pharmaceutical preparation. The antioxidant activity was preliminarily evaluated through in vitro assays, further confirmed through ex vivo analysis on murine fibroblasts, and the profile of phenol compounds was characterized. Results: Myrtle berries homogenate, containing yeast extract (0.4%, wt/vol), was fermented with Lactobacillus plantarum C2, previously selected from plant matrix. Chemically acidified homogenate, without bacterial inoculum and incubated under the same conditions, was used as the control. Compared to the control, fermented myrtle homogenate exhibited a marked antioxidant activity in vitro. The radical scavenging activity towards DPPH increased by 30%, and the inhibition of linoleic acid peroxidation was twice. The increased antioxidant activity was confirmed using Balb 3 T3 mouse fibroblasts, after inducing oxidative stress, and determining cell viability and radical scavenging activity through MTT and DCFH-DA assays, respectively. The lactic acid fermentation allowed increased concentrations of total phenols, flavonoids and anthocyanins, which were 5–10 times higher than those found for the non-fermented and chemically acidified control. As shown by HPLC analysis, the main increases were found for gallic and ellagic acids, and flavonols (myricetin and quercetin). The release of these antioxidant compounds would be strictly related to the esterase activities of L. plantarum. Conclusions: The lactic acid fermentation of myrtle berries is a suitable tool for novel applications as functional food dietary supplements or pharmaceutical preparations

The growth pattern of transplanted normal and nodular hepatocytes

Overt neoplasia is often the end result of a long biological process beginning with the appearance of focal lesions of altered tissue morphology. While the putative clonal nature of focal lesions has often been emphasized, increasing attention is being devoted to the possible role of an altered growth pattern in the evolution of carcinogenesis. Here we compare the growth patterns of normal and nodular hepatocytes in a transplantation system that allows their selective clonal proliferation in vivo. Rats were pre-treated with retrorsine, which blocks the growth of resident hepatocytes, and were then transplanted with hepatocytes isolated from either normal liver or hepatocyte nodules. Both cell types were able to proliferate extensively in the recipient liver, as expected. However, their growth pattern was remarkably different. Clusters of normal hepatocytes integrated in the host liver, displaying a normal histology; however, transplanted nodular hepatocytes formed new hepatocyte nodules, with altered morphology and sharp demarcation from surrounding host liver. Both the expression and distribution of proteins involved in cell polarity, cell communication, and cell adhesion, including connexin 32, E-cadherin, and matrix metalloproteinase-2, were altered in clusters of nodular hepatocytes. Furthermore, we were able to show that down-regulation of connexin 32 and E-cadherin in nodular hepatocyte clusters was independent of growth rate. These results support the concept that a dominant pathway towards neoplastic disease in several organs involves defect(s) in tissue pattern formation