Combining heuristics with simulation and fuzzy logic to solve a flexible-size location routing problem under uncertainty

The location routing problem integrates both a facility location and a vehicle routing problem. Each of these problems are NP-hard in nature, which justifies the use of heuristic-based algorithms when dealing with large-scale instances that need to be solved in reasonable computing times. This paper discusses a realistic variant of the problem that considers facilities of different sizes and two types of uncertainty conditions. In particular, we assume that some customers’ demands are stochastic, while others follow a fuzzy pattern. An iterated local search metaheuristic is integrated with simulation and fuzzy logic to solve the aforementioned problem, and a series of computational experiments are run to illustrate the potential of the proposed algorithm.This work has been partially supported by the Spanish Ministry of Science (PID2019-111100RB-C21/AEI/10.13039/501100011033). In addition, it has received the support of the Doctoral School at the Universitat Oberta de Catalunya (Spain) and the Universidad de La Sabana (INGPhD-12-2020).Peer ReviewedPostprint (published version

Eficiencia espectral y capacidad en un canal MIMO masivo con alta densidad de usuarios

[EN] This paper presents an analysis of the massive MIMO channel in an indoor picocell with a high number of active user terminals. The analysis is based on the results of a measurement campaign carried out in the 3.2 to 4 GHz band in a scenario of reduced size, in which users (up to 20) are placed in an orderly manner. In order to evaluate the performance of the resulting massive MIMO channel, results concerning both the sum capacity as well as the spectral efficiency are included. Furthermore and concerning the channel spectral efficiency, an analysis showing the spread and differences between the individual contributions of each active user is reported and discussed.Este trabajo ha sido financiado por los proyectos del Plan Estatal de Investigación Científica y Técnica y de Innovación TEC2017-86779-C2-1-R y TEC2017-86779-C2-2-R.Alejandro Rodríguez Aparicio; Jesús R. Pérez; Luis Valle; Torres Jiménez, RP.; Rubio Arjona, L.; Rodrigo Peñarrocha, VM.; Reig, J. (2021). Eficiencia espectral y capacidad en un canal MIMO masivo con alta densidad de usuarios. Íñigo Cuiñas Gómez. 1-4. http://hdl.handle.net/10251/1910821

Análisis experimental de un canal massive MIMO en una picocelda de interior

[EN] This paper presents an analysis of the massive MIMO channel in an indoor picocell. The analysis is based on the results of a measurement campaign carried out in the 3.2 to 4 GHz band in a scenario of reduced size and with a symmetrical geometry. It is well known that the performance of massive MIMO systems depends largely on the fact that the radio channel meets the condition of favorable propagation. In order to evaluate the performance of the resulting massive MIMO channel, results concerning the sum capacity are included. A second factor that determines the performance of massive MIMO systems when it is operated in a TDD-OFDM framework is the frequency selectivity of the channel that limits the size of the coherence block. In this sense, results of the coherence bandwidths achieved are also presented and analyzed.Este trabajo ha sido financiado por los proyectos del Plan Estatal de Investigación Científica y Técnica y de Innovación TEC2017-86779-C2-1-R y TEC2017-86779-C2-2-R.Torres, RP.; Pérez, JR.; Basterrechea, J.; Valle, L.; Domingo, M.; Rubio Arjona, L.; Rodrigo Peñarrocha, VM.... (2020). Análisis experimental de un canal massive MIMO en una picocelda de interior. Universidad de Málaga. 1-4. http://hdl.handle.net/10251/178585S1

Modelado del scattering difuso en la banda de milimétricas

[EN] Propagation channel models based on ray-tracing techniques can estimate, with some details, the specular multipath components reaching the receiver antenna, but have difficulty to estimate diffuse components. In this paper, an empirical model to include diffuse scattering in ray-tracing channel models, or channel simulators, is proposed. The diffuse scattering model has been developed from channel measurements collected from 25 to 40 GHz in the frequency domain, in both line-of-sight (LOS) and obstructed-LOS (OLOS) propagation conditions, in an office environment. The accuracy of a raytracing software to estimate time-dispersion metrics is evaluated when the diffuse scattering model is taken into account.Este trabajo ha sido financiado en parte por el Ministerio de Economía, Industria y Competitividad a través de los proyectos TEC2017-86779-C2-1-R y TEC2017-86779-C2-2- R, a través de la Agencia Estatal de Investigación (AEI) y el Fondo Europeo de Desarrollo Regional (FEDER).Andani, FJ.; Rubio Arjona, L.; Bernardo-Clemente, B.; Rodrigo Peñarrocha, VM.; Reig, J.; Pérez, JR.; Torres, RP.... (2021). Modelado del scattering difuso en la banda de milimétricas. Íñigo Cuiñas Gómez. 1-4. http://hdl.handle.net/10251/1910801

Regeneración de plantas via embriogénesis somática en Phaseolus acutifolius A. Gray

Plant regeneration protocols are a requirement to develop plants transformation systems.The capacity of Phaseolus acutifolius A. Gray to regenerate plants from embryogenic callus formation was investigated. Two explants were used to form calli on a culture medium containing thidiazuron and indole-3-acetic acid. The embryonic axes showed better capacity than cotyledons to form embryos. Solar light was the most favourable to develop the in vitro plants. The 32% of somatic embryos with complete germination was achieved. These results indicate that somatic embryos formation and their germination in Phaseolus acutifolius A. Gray depended on the explant, culture medium and illumination conditions. In vitro-germinated plantlets were established in the greenhouse.Key words: bean, callus formation, plant regeneration, tissue culturePara desarrollar un sistema de transformación genética un requisito fundamental es contar con un protocolo de regeneración de plantas. En el trabajo se investigó la capacidad de Phaseolus acutifolius para regenerar plantas a partir de callos con estructuras embriogénicas. Se utilizaron dos tipos de explantes para la formación de callos en un medio de cultivo que contenía thidiazuron y ácido indol-acético. Los ejes embrionarios mostraron una mejor capacidad que los cotiledones para la formación de embriones somáticos. La luz solar resultó ser la más favorable para el desarrollo de las plantas in vitro. En estas condiciones se alcanzó el 32% de los embriones somáticos con germinación completa. Estos resultados indicanron que la formación de embriones somáticos y su germinación en Phaseolus acutifolius dependieron del explante, el medio de cultivo y las condiciones de iluminación. Las plantas germinadas in vitro, pudieron ser aclimatizadas en el invernadero.Palabras clave: cultivo de tejidos, formación de callos, frijol, regeneración de plantasAbbreviations: BAP 6- Benzylaminopurine, TDZ Thidiazuron, IAA indole-3-acetic acid, GA3 gibberellic acid, AgNO3 silver nitrat

Suppressing aberrant GluN3A expression rescues synaptic and behavioral impairments in Huntington's disease models

Huntington's disease is caused by an expanded polyglutamine repeat in the huntingtin protein (HTT), but the pathophysiological sequence of events that trigger synaptic failure and neuronal loss are not fully understood. Alterations in N-methyl-D-aspartate (NMDA)-type glutamate receptors (NMDARs) have been implicated. Yet, it remains unclear how the HTT mutation affects NMDAR function, and direct evidence for a causative role is missing. Here we show that mutant HTT redirects an intracellular store of juvenile NMDARs containing GluN3A subunits to the surface of striatal neurons by sequestering and disrupting the subcellular localization of the endocytic adaptor PACSIN1, which is specific for GluN3A. Overexpressing GluN3A in wild-type mouse striatum mimicked the synapse loss observed in Huntington's disease mouse models, whereas genetic deletion of GluN3A prevented synapse degeneration, ameliorated motor and cognitive decline and reduced striatal atrophy and neuronal loss in the YAC128 Huntington's disease mouse model. Furthermore, GluN3A deletion corrected the abnormally enhanced NMDAR currents, which have been linked to cell death in Huntington's disease and other neurodegenerative conditions. Our findings reveal an early pathogenic role of GluN3A dysregulation in Huntington's disease and suggest that therapies targeting GluN3A or pathogenic HTT-PACSIN1 interactions might prevent or delay disease progression

Bona fide atypical scrapie faithfully reproduced for the first time in a rodent model

Atypical Scrapie, which is not linked to epidemics, is assumed to be an idiopathic spontaneous prion disease in small ruminants. Therefore, its occurrence is unlikely to be controlled through selective breeding or other strategies as it is done for classical scrapie outbreaks. Its spontaneous nature and its sporadic incidence worldwide is reminiscent of the incidence of idiopathic spontaneous prion diseases in humans, which account for more than 85% of the cases in humans. Hence, developing animal models that consistently reproduce this phenomenon of spontaneous PrP misfolding, is of importance to study the pathobiology of idiopathic spontaneous prion disorders. Transgenic mice overexpressing sheep PrPC with I112 polymorphism (TgShI112, 1–2 × PrP levels compared to sheep brain) manifest clinical signs of a spongiform encephalopathy spontaneously as early as 380 days of age. The brains of these animals show the neuropathological hallmarks of prion disease and biochemical analyses of the misfolded prion protein show a ladder-like PrPres pattern with a predominant 7–10 kDa band. Brain homogenates from spontaneously diseased transgenic mice were inoculated in several models to assess their transmissibility and characterize the prion strain generated: TgShI112 (ovine I112 ARQ PrPC), Tg338 (ovine VRQ PrPC), Tg501 (ovine ARQ PrPC), Tg340 (human M129 PrPC), Tg361 (human V129 PrPC), TgVole (bank vole I109 PrPC), bank vole (I109I PrPC), and sheep (AHQ/ARR and AHQ/AHQ churra-tensina breeds). Our analysis of the results of these bioassays concludes that the strain generated in this model is indistinguishable to that causing atypical scrapie (Nor98). Thus, we present the first faithful model for a bona fide, transmissible, ovine, atypical scrapie prion disease.info:eu-repo/semantics/publishedVersio

Bona fide atypical scrapie faithfully reproduced for the first time in a rodent model

Atypical Scrapie, which is not linked to epidemics, is assumed to be an idiopathic spontaneous prion disease in small ruminants. Therefore, its occurrence is unlikely to be controlled through selective breeding or other strategies as it is done for classical scrapie outbreaks. Its spontaneous nature and its sporadic incidence worldwide is reminiscent of the incidence of idiopathic spontaneous prion diseases in humans, which account for more than 85% of the cases in humans. Hence, developing animal models that consistently reproduce this phenomenon of spontaneous PrP misfolding, is of importance to study the pathobiology of idiopathic spontaneous prion disorders. Transgenic mice overexpressing sheep PrPC with I112 polymorphism (TgShI112, 1–2 × PrP levels compared to sheep brain) manifest clinical signs of a spongiform encephalopathy spontaneously as early as 380 days of age. The brains of these animals show the neuropathological hallmarks of prion disease and biochemical analyses of the misfolded prion protein show a ladder-like PrPres pattern with a predominant 7–10 kDa band. Brain homogenates from spontaneously diseased transgenic mice were inoculated in several models to assess their transmissibility and characterize the prion strain generated: TgShI112 (ovine I112 ARQ PrPC), Tg338 (ovine VRQ PrPC), Tg501 (ovine ARQ PrPC), Tg340 (human M129 PrPC), Tg361 (human V129 PrPC), TgVole (bank vole I109 PrPC), bank vole (I109I PrPC), and sheep (AHQ/ARR and AHQ/AHQ churra-tensina breeds). Our analysis of the results of these bioassays concludes that the strain generated in this model is indistinguishable to that causing atypical scrapie (Nor98). Thus, we present the first faithful model for a bona fide, transmissible, ovine, atypical scrapie prion disease.info:eu-repo/semantics/publishedVersio

Modelado de las pérdidas de propagación en un escenario de oficinas en bandas de milimétricas

[EN] In this conference contribution, we present millimeter-wave (mmWave) propagation measurements for an office environment from 25 to 40 GHz and investigate the path loss using the most common empirical models proposed in WINNER-II and adopted by the 3GPP: the single-frequency floating-intercept (FI) model, the single-frequency close-in (CI) free space reference distance model, the multi-frequency alphabeta-gamma (ABG) model and the close-in free space reference distance with frequency path loss exponent (CIF). Both line-ofsight (LOS) and obstructed-LOS (OLOS) propagation conditions have been considered. The mean value of the model parameters, and their 95 % confidence intervals, have been derived using regression and optimization techniques.Este trabajo ha sido financiado en parte por el Ministerio de Economia, Industria y Competitividad a traves de los proyectos TEC2016-78028-C3-2-P, TEC2017-86779-C2-1-R y TEC2017-86779-C2-2-R, a traves de la Agencia Estatal de Investigacion (AEI) y el Fondo Europeo de Desarrollo Regional (FEDER) y por COLCIENCIAS en Colombia.Rubio Arjona, L.; Bernardo-Clemente, B.; Rodrigo Peñarrocha, VM.; Reig, J.; Molina-García-Pardo, JM.; Pérez, JR.; Torres, RP.... (2020). Modelado de las pérdidas de propagación en un escenario de oficinas en bandas de milimétricas. Universidad de Málaga. 1-4. http://hdl.handle.net/10251/178596S1