Inhibition of an extracellular polygalacturonase from Geotrichum candidum by a proteinaceous inhibitor isolated from lemon fruits

An extracellular polygalacturonase [EC 3.2.1.15], named PG-543, was purified from the culture of Geotrichum candidum IEV 543, a phytopathogenic fungi isolated from infected lemon. The molecular mass of the enzyme was estimated to be 26 kDa by SDS-PAGE. The pH optimum determined was 5.5; the enzyme showed high stability in the pH range between 4.0 and 6.0. The temperature optimum was 37 ºC. Furthermore, a proteinaceous inhibitor of the polygalacturonase (PG) named PGIP was isolated from lemon albedo. This protein was able to maintain the inhibitory activity in a wide range of pH and temperature. The maximal interaction between PG-PGIP was reached after 20 min of contact. PGIP did not show toxic effect on Artemia salina in the concentration range that was active on PG enzyme. Also, the proteinaceous inhibitor did not show mutagenic nor phytotoxic effects.Our results suggest that, the PGIP isolated from lemon could be used as a natural product to decrease the propagation of pathogenic fungi responsible for postharvest diseases in citrus by the inhibition of hydrolytic enzymes secreted by pathogens.Fil: Rodriguez, Ivana Fabiola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; ArgentinaFil: Torres, Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaFil: Sayago, Jorge Esteban. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaFil: Zampini, Iris Catiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaFil: Isla, Maria Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; ArgentinaFil: Ordóñez, Roxana Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Química del Noroeste. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Química del Noroeste; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Naturales e Instituto Miguel Lillo; Argentin

How Do Millennials Learn? An Investigation of the Learning Styles, Strategies, and Perceived Academic Success Among College-aged Millennials

Purpose: Research shows Millennials learn better by actively doing things than listening to lectures. However, there is little research on millennials and how they learn or what drives them towards success. The purpose of this study was to investigate the preferred learning styles of undergraduate college students and their perception of academic success. Methods: Data collection included quantitative and qualitative measures that were used to collect data from a convenience sample of 344 undergraduate college students in a rural southeast regional university. Study was IRB approved prior to data collection. Data were analyzed using appropriate descriptive statistics, cross-tabulations, and coding of qualitative data. Results: Findings indicated that 35.4% of the 344 participants self-reported as kinesthetic (hands-on) learners compared to visual (23.6%), auditory (17%), and read/write (24%) learners. Findings also revealed that only 12.9% of the participants “spend 3 or more hours a day outside of class studying; 34% “ask for help when they do not understand something in class”; 41.1% “feel comfortable approaching professor for extra help” and 60.6% “prefer a teacher who incorporates hands-on activities with lecture.” Millennials in this study defined academic success as (1) establishing effective learning habits, (2) attending class regularly to maintain consistent performance, (3) achieving academic goals, and (4) making good grades. Conclusion: Incorporating kinesthetic activities in the classroom setting will positively enhance learning outcomes and academic success of millennial college students

msBiodat analysis tool, big data analysis for high-throughput experiments

Background Mass spectrometry (MS) are a group of a high-throughput techniques used to increase knowledge about biomolecules. They produce a large amount of data which is presented as a list of hundreds or thousands of proteins. Filtering those data efficiently is the first step for extracting biologically relevant information. The filtering may increase interest by merging previous data with the data obtained from public databases, resulting in an accurate list of proteins which meet the predetermined conditions. Results In this article we present msBiodat Analysis Tool, a web-based application thought to approach proteomics to the big data analysis. With this tool, researchers can easily select the most relevant information from their MS experiments using an easy-to-use web interface. An interesting feature of msBiodat analysis tool is the possibility of selecting proteins by its annotation on Gene Ontology using its Gene Id, ensembl or UniProt codes. Conclusion The msBiodat analysis tool is a web-based application that allows researchers with any programming experience to deal with efficient database querying advantages. Its versatility and user-friendly interface makes easy to perform fast and accurate data screening by using complex queries. Once the analysis is finished, the result is delivered by e-mail. msBiodat analysis tool is freely available at http://msbiodata.irb.h

Contamination of resin-matrix composites on chairside handling using latex or nitrile gloves: an in vitro study

The aim of this study was to evaluate the efect of diferent gloves and clinical environment on the contamination of resinmatrix composites for restorative dentistry. Specimens of nano-hybrid resin-matrix composite (n=6) were divided in groups regarding the handling with (A) clinical spatula; (B) latex gloves; (C) latex or (D) nitrile gloves with human saliva; (E) latex or (F) nitrile gloves with human blood. After light curing, groups of specimens were analyzed by optical microscopy at magnifcation ranging from x30 up to x500 and by scanning electron microscopy at diferent magnifcation ranging from x50 up to x8000. Handling of resin-matrix composites with unpowdered nitrile gloves or clinical spatulas avoided the presence of contaminants. However, agglomerates of the resin-matrix composite itself became entrapped leading to a heterogenous morphological aspect. SEM images revealed the presence of corn-derived starch released from the powdered gloves. Also, the formation of micro-spaces (voids) occurred after handling with powdered latex gloves. Specimens handled with both type of gloves contaminated with saliva showed a conditioning layer composed of glycoproteins rolls and compounds including calcium-based chlorides, phosphates, and carbonates. Also, blood products were transferred from the contaminated gloves to the resin-matrix composites after handling. Thus, resin-matrix composite restorations are susceptible to contamination with debris from powdered gloves. Also, saliva or blood debris become adsorbed and entrapped in the resin-matrix composites during clinical handling leading to the presence of defects such macro- and micro-scale voids or contaminant agglomerates.info:eu-repo/semantics/publishedVersio

Santa Ana la Aguadora

EmprendimientoEl sendero Santa Ana – la Aguadora buscar generar a la sociedad sensibilidad, amor, entusiasmo, compromiso, y sobre todo respeto con el medio ambiente.INTRODUCCIÓN 1.DIAGNÓSTICO GENERAL 2. TAMAÑO DEL MERCADO 3. CONCLUSIONES BIBLIOGRAFÍA ANEXOSPregradoEconomist

Síndrome de Burnout en médicos residentes de un hospital público de la ciudad de Aregua en el año 2020

Introducción: El estrés merece especial atención dentro de lo que sería la salud mental, teniendo en cuenta que estados prolongados de estrés podrían acarrear estados depresivos o de ansiedad severos. Las labores hospitalarias son uno de los trabajos de fuente innata para el estrés, este trabajo se enfocará en el estrés en médicos residentes. Objetivo: Determinar el síndrome de Burnout en médicos residentes de un hospital público de la ciudad de Areguá en el año 2020. Material y Método: Estudio observacional descriptivo, de corte transversal, muestra no probabilístico por conveniencia, desde junio hasta agosto de 2020, de 24 encuestas, en el Hospital Nacional del Cáncer (Aregua). Se aplicó Cuestionario de Maslach Burnout Inventory- Healt Service Survey (MBI-HSS). Resultados: Se analizaron 24 encuestas. Edades comprendidas entre 24 y 34 años (28,875±3,0405).  Se reportan a 12 residentes con Síndrome de Burnout, equivalente al 54,2% de la muestra. Realización personal baja con 66,7% (16); Agotamiento emocional alto 45,8% (11); Despersonalización alta 50% (12). Discusión: En la presente investigación se encontró que el 54,2% de los residentes encuestados son categorizados con Sindrome de Burnout. Se resalta la investigación de Jacome et al donde el 47,5% de los residentes fueron categorizados con Síndrome de Burnout; mientras que, en el estudio de Estigarribia et al se encontró una baja prevalencia del Sindrome de Burnout, equivalente al 22%; esta diferencia se puede deber al instrumento que fue utilizado en ambos estudios, tratándose del MBI

Propuestas Metodológicas para el Establecimiento de un Score de Crédito a Partir de Metodologías de Componentes Principales

Los modelos de score de crédito son instrumentos que generan calificaciones según el historial y comportamiento crediticio del individuo, estos son utilizados por las entidades financieras para caracterizar a sus clientes. Los cuales ayudan en la toma de la decisión para el otorgamiento de un crédito evitando que se generen perdidas por el incumplimiento por parte de sus clientes. La superintendencia Bancaria de Colombia, ahora Superintendencia Financiera de Colombia, a través de la circular Externa 11 y de la carta Circular 31 de marzo de 2002, desarrolló un nuevo mecanismo de gestión del riesgo de crédito para aquella entidad vigilada por medio del Sistema de Administración de riesgo crediticio (SARC). El objetivo de este sistema es fortalecer la medición y control del riesgo de crédito, exponiendo la libertad para cada una de las entidades financieras de desarrollar modelos propios para mitigar este riesgo anteriormente nombrado. El objetivo de este trabajo es la elaboración de un modelo de score de crédito dadas las características cualitativas y cuantitativas de vida de una persona enfocándose en los años 2018 y 2019, utilizando información de tipo poblacional, suministrada en la encuesta de calidad de vida (ECV) que realiza anualmente el Departamento Administrativo Nacional de Estadística (DANE) aplicando como herramienta estadística la metodología de componentes principales, la cual busca minimizar la variabilidad a partir de una combinación lineal. No obstante, esta metodología no permite calcular probabilidades de ocurrencia de default, en tanto la finalidad de este documento es realizar una propuesta no solamente para minimizar la varianza sino tratar de generar una aproximación a una medición de puntaje de crédito. A partir de la aplicación de dicha metodología, se evidenció que en promedio la población tiene características similares en los años 2018 y 2019, siendo en su mayoría mujeres, que saben leer y escribir, con nivel educativo en promedio de básica primaria. Por su parte, el ingreso es la variable que presenta mayor variación ubicándose en un rango de $190.000 a$710.000 aproximadamente para el año 2018 y de $275.000 a$1.030.000 para el siguiente año. Por otro lado, se determinó a través del desarrollo de la matriz de transición, que la mayoría de la población empeoró en su calidad crediticia al pasar de un año al otro.Credit score models are instruments that generate ratings according to the credit history and behavior of the individual, these are used by financial entities to characterize their clients. Which help in making the decision to grant a loan, preventing losses from being generated due to non-compliance by their clients. The Banking Superintendence of Colombia, now the Financial Superintendence of Colombia, through External Circular Letter 11 and Circular Letter March 31, 2002, developed a new credit risk management mechanism for that entity supervised by means of the Administration System. of credit risk (SARC). The objective of this system is to strengthen the measurement and control of credit risk, exposing the freedom for each of the financial entities to develop their own models to mitigate this aforementioned risk. The objective of this work is the elaboration of a credit score model given the qualitative and quantitative characteristics of a person's life, focusing on the years 2018 and 2019, using population-type information, provided in the quality of life survey (ECV).) carried out annually by the National Administrative Department of Statistics (DANE) applying the principal components methodology as a statistical tool, which seeks to minimize variability from a linear combination. However, this methodology does not allow calculating probabilities of occurrence of default, while the purpose of this document is to make a proposal not only to minimize the variance but also to try to generate an approximation to a credit score measurement. From the application of said methodology, it was evidenced that on average the population has similar characteristics in the years 2018 and 2019, being mostly women, who know how to read and write, with an average educational level of basic primary. For its part, income is the variable that presents the greatest variation, ranging from $190,000 to$710,000 approximately for the year 2018 and from $275,000 to$1,030,000 for the following year. On the other hand, it was determined through the development of the transition matrix, that the majority of the population worsened in their credit quality as they passed from one year to the next

Avaliação fisioterápica em pacientes pós cirurgia de câncer de mama em Joinville/SC

O câncer de mama é uma neoplasia maligna e também a principal causa morte entre as mulheres. Com o aparecimento do câncer (CA) de mama surgem diversas alterações na vida das mulheres acometidas, principalmente após a cirurgia, elas passam a ter uma nova realidade corporal, pois ocorrem alterações importantes em nível anatômico, fisiológico e funcional. O objetivo do presente trabalho foi identificar as alterações, seqüelas, limitações e complicações através de avaliações fisioterápicas em indivíduos que foram submetidos a cirurgias de CA de mama pelo Sistema Único de Saúde (SUS) na cidade de Joinville/SC no período de 2007 a 2010. Foram avaliadas 36 mulheres acometidas pelo CA de mama e submetidas à cirurgia. Os dados foram obtidos por entrevistas, exame físico e revisão de prontuários fornecidos pelo Hospital Municipal São José de Joinville/SC. Dos resultados encontrados 88,9% apresentou o carcinoma ductal invasivo, a mastectomia radial modificada com 33,4%, a hipoestesia foi à maior alteração de sensibilidade encontrada, também foi diagnosticado restrição na amplitude de movimento, presença de edema, linfedema, fibrose e aderência, a atividade funcional de maior dificuldade em realizar foi de carregar sacolas. Apesar da gravidade que a lesão neoplásica representa e das complicações apresentadas foi encontrado índice maior de normalidade em relação às complicações e alterações no pós-cirúrgico. Acredita-se que esta normalidade foi atribuída a orientações recebidas pela equipe médica, em realizar após a cirurgia algum tipo de exercício físico ou fisioterapia para acelerar seu retorno as atividades funcionais, melhorando a qualidade de vida e evitando assim suas complicações

Increased expression of markers of early atherosclerosis in patients with inflammatory bowel disease

Recent studies documented an increased cardiovascular risk in patients with inflammatory bowel disease (IBD). Our study aimed at investigating the prevalence of intima-media thickness (IMT) of the carotid arteries and the arterial stiffness indices as markers of early atherosclerosis in young IBD patients