SANS study of hybrid silica aerogels under "in situ" uniaxial compression

We have modified the inorganic silica network of aerogels with polydimethylsiloxane (PDMS), a hydroxyl-terminated polymer, to obtain an organic modified silicate (ORMOSIL). Reactions were assisted by high-power ultrasounds. The resulting gels were dried under supercritical conditions of the solvent to obtain a monolithic sono-aerogel. The mechanical behaviour of these aerogels can be tuned from brittle to rubbery as a function of the organic polymer content. In order to determine the links between the mechanical behaviour and modifications made to the microstructure, SANS (small-angle neutron scattering) experiments were carried out. To measure the intensities under "in situ" uniaxial compression of the aerogel, a specific sample-holder was built. Under uniaxial compression the 2D-diagrams were significantly anisotropic (butterfly pattern), indicating the rearrangement of the polymer. The form factor of these aerogels is described well by two correlation lengths, small microporous silica clusters surrounded by entangled polymer chains of 6 nm average size (blobs), which form a larger secondary level of agglomerates governed by the "frozen-in" elastic constraints.Comisión Interministerial de Ciencia y Tecnología MAT2005-1583European Commission CT-2003-50592

Bioactivity of wollastonite/aerogels composites obtained from a TEOS-MTES matrix

Organic-inorganic hybrid materials were synthesized by controlled hydrolysis of tetraethoxysilane (TEOS), methyltrimethoxysilane (MTES), synthetic wollastonite powders and polydimethylsiloxane (PDMS) in an ethanol solution. Aerogels were prepared from acid hydrolysis of TEOS and MTES with different volume ratio in ethanol, followed by addition of wollastonite powder and PDMS in order to obtain aerogels with 20 wt% of PDMS and 5 wt% of CaO of the total silica. Finally, when the wet gels were obtained, they were supercritically dried at 260°C and 90 bar, in ethanol. In order to obtain its bioactivity, one method for surface activation is based on a wet chemical alkaline treatment. The particular interest of this study is that we introduce hybrid aerogels, in a 1 M solution of NaOH, for 30 s at room temperature. We evaluate the bioactivity of TEOS-MTES aerogel when immersed in a static volume of simulated body fluid (SBF). An apatite layer of spherical-shaped particles of uniform size smaller than 5 microns is observed to form on the surface of the aerogels after 25 days soaking in SBF.Ministerio de Ciencia e Innovación MAT2005-01583Junta de Andalucía TEP 79

HI asymmetry in the isolated galaxy CIG 85 (UGC 1547)

We present the results from the Giant Metrewave Radio Telescope (GMRT) interferometric HI and 20 cm radio continuum observations of CIG 85, an isolated asymmetric galaxy from the AMIGA (Analysis of the Interstellar Medium of Isolated GAlaxies) sample. Despite being an isolated galaxy, CIG 85 showed an appreciable optical and HI spectral asymmetry and therefore was an excellent candidate for resolved HI studies to understand the reasons giving rise to asymmetries in isolated galaxies. The galaxy was imaged in HI and 20 cm radio continuum using the GMRT. For a detailed discussion of the results we also made use of multi-wavelength data from archival SDSS, GALEX and Halpha imaging. We find the HI in CIG 85 to have a clumpy, asymmetric distribution which in the NW part is correlated with optical tail like features, but the HI velocity field displays a relatively regular rotation pattern. Evaluating all the observational evidence, we come to a conclusion that CIG 85 is most likely a case of a disturbed spiral galaxy which now appears to have the morphology of an irregular galaxy. Although it is currently isolated from major companions, the observational evidence is consistent with HI asymmetries, a highly disturbed optical disk and recent increase in star formation having been caused by a minor merger, remnants of which are now projected in front of the optical disk. If this is correct, the companion will be fully accreted by CIG 85 in the near future.Comment: 10 pages, 9 figures, accepted in A&

Monitoring synaptic function at the neuromuscular junction of a mouse expressing synaptopHluorin

We monitored presynaptic exocytosis and vesicle recycling at neuromuscular junctions of transgenic mice expressing synaptopHluorin (spH), using simultaneous optical and electrophysiological recordings. Synaptic transmission was indistinguishable from that in wildtype controls. Fluorescence rose during and decayed monotonically after stimulus trains to the nerve, with amplitudes and decay times increasing with the amount of stimulation. The relatively large size of synaptic terminals allowed us to examine the spatial profile of fluorescence changes. We identified hot spots of exocytosis, which were stable with repeated trains. Photobleach experiments showed that spH freshly exposed by nerve stimulation was not preferentially retrieved by compensatory endocytosis; instead, most retrieved spH preexisted in the surface membrane. Finally, we compared fluorescence and electrical [summed end-plate potentials (EPPs)] estimates of exocytosis, which diverged during repeated trains, as fluorescence exceeded summed EPPs, although the average amplitude of miniature EPPs was unchanged. This might reflect exocytosis of spH-containing, acetylcholine-free (“empty”) vesicles or other organelles during intense stimulation

Detección y prácticas de manejo de la enfermedad pata prieta causada por Phytophthora Nicotianae en el cultivo del tabaco

Tobacco (Nicotiana tabacum L.) is one of the most important economic factors in Cuba. The second disease of importance is the black shank caused by Phytophthora nicotianae Breda de Hann. From 1985-1986 it’s incidence increased, although Metyl Bromide and Metalaxyl were used, the method control was deficient, that’s why several studies were carried out, one of there was the search for a detection and quantification method of soil pathogens, and other control alter- natives such as, area selection, crops rotation and the use of the Trichoderma biocontrol in soils. For the quantification of the pathogen in soils it was proved that the modification of the selective medium described by Kannwischer y Mitchell (1978) was efficient to determine the contamina- tion level of the soils in the fields, thus they were high in the plantations (3-39,5 propagules/g of soil ) and not in the fields used as seedbeds in which the pathogen was not detected in soils.This method allows us to select those fields for crops in which the pathogen was not detected in soils. This method allows us to select those fields for crops in which the pathogen is not detected in soil, or in which the contamination level is too low, thus avoiding the development of epidemics. Besides it was tested that the high density of sowing favored the development of the disease, and that area selection and crop rotation for 3 years, also the use of Trichoderma biocontrol were effective to diminish the damage of black shank in this crop.El tabaco (Nicotiana tabacum L.) es uno de los principales renglones económicos de Cuba. La segunda enfermedad de importancia es la pata prieta causada por Phytophthora nicotianae Breda de Hann. Desde 1985-1986 su incidencia fue en ascenso, y aunque se empleaban el bromuro de metilo y el metalaxyl el control era deficiente, por lo que se realizaron estudios, entre ellos la búsqueda de un método de detección y cuantificación del patógeno en suelo, así como alternativas de control como la selección de áreas, la rotación de cultivos y la aplicación del biocontrol Trichoderma al suelo. Para la cuantificación del patógeno en suelos se demostró que la modificación al medio selectivo descrito por Kannwischer y Mitchell (1978) fue eficaz para determinar el nivel de contaminación de los suelos de los campos, que fueron elevados en las plantaciones (3-39,5 propágulos/g de suelo), no así para los campos destinados para semilleros en que el patógeno no fue detectado en el suelo. Este método permite seleccionar para las siembras aquellos campos donde no se detecte el patógeno en suelo o en niveles de contaminación muy bajos, evitando el desarrollo de epidemias. Además se comprobó que las altas densidades de siembra favorecieron el desarrollo de la enfermedad, y que la selección de áreas y la rotación de cultivos por tres años, así como el empleo del biocontrol Trichoderma fueron efectivos para la disminución de las afectaciones por pata prieta en este cultivo

Factors associated with successful dietary changes in an energy-reduced Mediterranean diet intervention: a longitudinal analysis in the PREDIMED-Plus trial

Purpose Long-term nutrition trials may fail to respond to their original hypotheses if participants do not comply with the intended dietary intervention. We aimed to identify baseline factors associated with successful dietary changes towards an energy-reduced Mediterranean diet (MedDiet) in the PREDIMED-Plus randomized trial. Methods Longitudinal analysis of 2985 participants (Spanish overweight/obese older adults with metabolic syndrome) randomized to the active intervention arm of the PREDIMED-Plus trial. Dietary changes were assessed with a 17-item energy-reduced MedDiet questionnaire after 6 and 12 months of follow-up. Successful compliance was defined as dietary changes from baseline of ≥ 5 points for participants with baseline scores < 13 points or any increase if baseline score was ≥ 13 points. We conducted crude and adjusted multivariable logistic regression models to identify baseline factors related to compliance. Results Consistent factors independently associated with successful dietary change at both 6 and 12 months were high baseline perceived self-efficacy in modifying diet (OR6-month: 1.51, 95% CI 1.25–1.83; OR12-month: 1.66, 95% CI 1.37–2.01), higher baseline fiber intake (OR6-month: 1.62, 95% CI 1.07–2.46; OR12-month: 1.62, 95% CI 1.07–2.45), having > 3 chronic conditions (OR6-month: 0.65, 95% CI 0.53–0.79; OR12-month: 0.76, 95% CI 0.62–0.93), and suffering depression (OR6-month: 0.80, 95% CI 0.64–0.99; OR12-month: 0.71, 95% CI 0.57–0.88). Conclusion Our results suggested that recruitment of individuals with high perceived self-efficacy to dietary change, and those who initially follow diets relatively richer in fiber may lead to greater changes in nutritional recommendations. Participants with multiple chronic conditions, specifically depression, should receive specific tailored interventions

Leisure-time physical activity, sedentary behavior, and risk of breast cancer: Results from the SUN (‘Seguimiento Universidad De Navarra’) project

Evidence is still limited on the influence of sedentary lifestyles on breast cancer (BC) risk. Also, prospective information on the combined effects of both sedentariness and leisure-time physical activity (LTPA) is scarce. We aimed to assess the association of higher sedentary behavior and LTPA (separately and in combination) with the risk of BC in a middle-aged cohort of university graduates. The SUN Project is a follow-up study initiated in 1999 with recruitment permanently open. Baseline assessments included a validated questionnaire on LTPA and sedentary habits. Subsequently, participants completed biennial follow-up questionnaires. Multivariable adjusted Cox models were used to estimate the hazard ratios (HR) for incident BC according to LTPA, TV-watching, the joint classification of both, and a combined 8-item multidimensional active lifestyle score. We included 10,812 women, with 11.8 years of median follow-up of. Among 115,802 women-years of follow-up, we confirmed 101 incident cases of BC. Women in the highest category of LTPA (>16.5 MET-h/week) showed a significantly lower risk of BC (HR = 0.55; 95% CI: 0.34–0.90) compared to women in the lowest category (≤6 MET/h-week). Women watching >2 h/d of TV sh owed a higher risk (HR = 1.67; 95% CI:1.03–2.72) than those who watched TV 2 h/d may substantially increase BC risk, independently of each other

Dual cholinergic signals regulate daily migration of hematopoietic stem cells and leukocytes.

Hematopoietic stem and progenitor cells (HSPCs) and leukocytes circulate between the bone marrow (BM) and peripheral blood following circadian oscillations. Autonomic sympathetic noradrenergic signals have been shown to regulate HSPC and leukocyte trafficking, but the role of the cholinergic branch has remained unexplored. We have investigated the role of the cholinergic nervous system in the regulation of day/night traffic of HSPCs and leukocytes in mice. We show here that the autonomic cholinergic nervous system (including parasympathetic and sympathetic) dually regulates daily migration of HSPCs and leukocytes. At night, central parasympathetic cholinergic signals dampen sympathetic noradrenergic tone and decrease BM egress of HSPCs and leukocytes. However, during the daytime, derepressed sympathetic noradrenergic activity causes predominant BM egress of HSPCs and leukocytes via β3-adrenergic receptor. This egress is locally supported by light-triggered sympathetic cholinergic activity, which inhibits BM vascular cell adhesion and homing. In summary, central (parasympathetic) and local (sympathetic) cholinergic signals regulate day/night oscillations of circulating HSPCs and leukocytes. This study shows how both branches of the autonomic nervous system cooperate to orchestrate daily traffic of HSPCs and leukocytes

Spanish Cell Therapy Network (TerCel) : 15 years of successful collaborative translational research

In the current article we summarize the 15-year experience of the Spanish Cell Therapy Network (TerCel), a successful collaborative public initiative funded by the Spanish government for the support of nationwide translational research in this important area. Thirty-two research groups organized in three programs devoted to cardiovascular, neurodegenerative and immune-inflammatory diseases, respectively, currently form the network. Each program has three working packages focused on basic science, pre-clinical studies and clinical application. TerCel has contributed during this period to boost the translational research in cell therapy in Spain, setting up a network of Good Manufacturing Practice-certified cell manufacturing facilities- and increasing the number of translational research projects, publications, patents and clinical trials of the participating groups, especially those in collaboration. TerCel pays particular attention to the public-private collaboration, which, for instance, has led to the development of the first allogeneic cell therapy product approved by the European Medicines Agency, Darvadstrocel. The current collaborative work is focused on the development of multicenter phase 2 and 3 trials that could translate these therapies to clinical practice for the benefit of patients