Properties of anaerobic bacteria from ferrosphere crucial for biofilm development

The purpose of this study was the investigation of biofilm-forming, hydrophobic, sulfidogenic and aggregative properties of sulfate-reducing bacteria Desulfovibrio oryzae strains in monocultures and associations with organic acid-producing bacteria Anaerotignum propionicum. Studies of biofilm formation on polypropylene surface by strains were carried out by a biofilm assay (indirect measurement of bacterial biofilm biomass by crystal violet adsorption/desorption), hydrogen sulfide production was determined by iodometric titration, aggregation - by aggregation test, hydrophobicity - by salt aggregation test. It was found that the studied strains of D. oryzae NUChC SRB1 and NUChC SRB2 are highly adhesive, have high sulfidogenic, aggregation and hydrophobic properties in the complete Postgate’s medium (with Fe2+). During the cultivation of the studied strains of D. oryzae with A. propionicum NUChC Sat1, a significant increase in aggregation (both in complete medium and without Fe2+) and hydrogen sulfide production by sulfate-reducing bacteria were observed. These properties indicate the potentially high biodegradable activity of D. oryzae monocultures NUChC SRB1 and NUChC SRB2 and their associations with A. propionicum NUChC Sat1. The observed increase in the sulfidogenic activity of D. oryzae in association with A. propionicum promotes the corrosion hazard of the studied bacterial association

Ukrajinski putopisni mediji: analiza tematskih kategorija

This article analyzes the content of Ukrainian travel media to determine their thematic orientation by utilizing content analysis and statistical methods. To verify the representation of different thematic categories in the travel media a two-stage research design was adopted. First, we conducted a survey, applying a random sampling method regarding the most important themes covered by different travel media. Respondents’ answers were analyzed and grouped into several categories: learning about the country, art, lifestyle, famous historical figures, nature and ecology, tips, tourism infrastructure, tourism development, marketing and trends. Second, a content analysis was conducted by using the method of distributive averages of the mode and median to calculate the share of each thematic category. The dominant travel media thematic categories demonstrated the following rating: 1) learning about the country, 2) tips, 3) lifestyle, 4) art, 5) nature and ecology, 6) famous historical figures, 7) tourism infrastructure, 8) marketing and trends, 9) tourism development.Koristeći analizu sadržaja i statističke metode, ovaj članak analizira ukrajinske putopisne medije kako bi utvrdio njihovo tematsko usmjerenje. Kako bismo utvrdili zastupljenost različitih tematskih kategorija, proveli smo istraživanje na dvije razine. Kao prvo, proveli smo anketu temeljenu na slučajnom uzorku o najvažnijim temama koje pokrivaju različiti mediji koji se bave putovanjima. Odgovore ispitanika analizirali smo i grupirali u nekoliko kategorija: učenje o zemlji, umjetnost, lifestyle, poznate povijesne osobe, priroda i ekologija, savjeti, turistička infrastruktura, turistički razvoj, marketing i trendovi. Kao drugo, proveli smo analizu sadržaja koristeći distributivne prosjeka moda i medijana kako bismo izračunali udio svake tematske kategorije. Dominantne tematske kategorije bile su rangirane na sljedeći način: 1) učenje o zemlji, 2) savjeti, 3) lifestyle, 4) umjetnost, 5) priroda i ekologija, 6) slavne povijesne osobe, 7) turistička infrastruktura, 8) marketing i trendovi, 9) turistički razvoj

Microbiological and genetic characteristics of Bacillus velezensis bacillibactinproducing strains and their effect on the sulfate-reducing bacteria biofilms on the poly(ethylene terephthalate) surface

. It was evaluated the antibiofilm-forming properties of NUChC C1 and NUChC C2b isolates (from the collection of the Department of Biology of the T.H. National University “Chernihiv Colehium”) against the sulfate-reducing bacteria biofilms on the poly(ethylene terephthalate) surface. NUChC C1 and NUChC C2b isolates were isolated by classical microbiology methods on Postgate’s “B” medium and their cultural-morphological, some physiological-biochemical properties and molecular-genetic characteristics were investigated. To identify bacteria the sequencing and analysis of the 16S rRNA gene were carried out. The bacteria were identified as Bacillus velezensis. Based on PCR-ISSR analysis, it was found that the studied bacteria belong to different strains. The 16S rRNA gene sequences were submitted in GenBank as MN508954.1 (NUChC C1), MN749356.1 and MN749357.1 (NUChC C2b). In the genome of B. velezensis the presence and transcriptional activity of the genes for the synthesis of bacillibactin (dhbC, dhbF), fengycin (fenA) and polyglutamic acid (epsK) were studied. Among these only genes belonging to bacillibactin synthesis operon were detected and only they demonstrated activity. The observed mode of dhbC and dhbF genes expression during 144 hours of cultivation differed between two B. velezensis strains: gradually increasing in NUChC C1 and sharply increased after 24 hours with decreasing on 144th hour in NUChC C2b. Antagonistic properties of the studied strains of B. velezensis against sulfate-reducing bacteria Desulfovibrio oryzae NUChC SRB1 and NUChC SRB2 were not observed. Siderophore-producing strains of Bacillus velezensis inhibit the formation of bacterial biofilms on the polymeric material poly(ethylene terephthalate) during its long-term exposure (50 days) in a culture of sulfatereducing bacteria under conditions of sufficient iron supply. Bacillibactin-producing strains prevent the development of bacterial biofilms on the poly(ethylene terephthalate) surface. This is one of the reasons for the prolongation of the process of poly(ethylene terephthalate) biodegradation in natural ecosystems

Genotypic, physiological and biochemical features of Desulfovibrio strains in a sulfidogenic microbial community isolated from the soil of ferrosphere

The purpose of this work was the isolation of the predominant representatives of sulfate-reducing bacteria (SRB) of the sulfidogenic microbial community separated from the soil ferrosphere and the examination of their morphological, physiological, biochemical and genotypic peculiarities, the evaluation of some physiological processes under co-culturing with their satellite species Anaerotignum propionicum. During the study two isolates of sulfatereducing bacteria NUChC SRB1 and NUChC SRB2 were obtained from sulfidogenic microbial community isolated from soil ferrosphere on Postgate’s “B” medium and their belonging to different strains (using ISSR-PCR method) was proved. As a result of molecular-genetic analysis of the strains, a 16S rRNA gene fragments of 613 bp and 522 bp were amplified and sequenced. The strains were identified as Desulfovibrio oryzae by the complex of microbiological, physiological and biochemical features and on the basis of 16S rRNA gene sequences (phylogenetic analysis). The 16S rRNA gene sequences were submitted in GenBank as MT102713 (NUChC SRB1) and MT102714 (NUChC SRB2). The co-cultivation of isolated SRB strains with A. propionicum NUChC Sat1 strain (in the absence of electron donors, the presence of sulfates and yeast extract) showed the formation of sulfur-reducing bacteria of hydrogen sulfide, which was not observed during their mono-cultivation. In this case, the phenomenon of syntrophy probably takes place- co-growth on the nutrient substrate, and the electron donor appears due to the use of the yeast extract compounds by the NUChC Sat1 strain. Therefore, in the sulfidogenic community isolated from the soil ferrosphere, there is a mutual growth of the association of bacteria D. oryzae and A. propionicum, which is caused by trophic interaction. Possibly the contribution of these associated bacteria to the corrosion process lies in the utilization of hydrogen (D. oryzae) and the formation of substrate products of SRB metabolism (hydrogen and organic acids), which are both corrosive compounds (A. propionicum). Without a doubt the corrosion process involving this association needs further investigation

O PROGRAMA DE INTEGRAÇÃO EUROPEIA DA UCRÂNIA NO CONTEXTO DA MODERNIZAÇÃO E ADAPTAÇÃO DA POLÍTICA EDUCATIVA: : ANÁLISE DO ESTADO ATUAL

The purpose of the article is to highlight the strategies for the development of Ukrainian education in the short and long term of the integration process into the European community. The task of scientific research is focused on the correlation of the processes of integration of Ukrainian education into the European paradigm. The research methodology is based on a combination of classical and innovative approaches of scientific and pedagogical discourse. The results of the study indicate the problematic aspects of the transition of Ukrainian education to the standards of the European educational system, namely, the oversaturation of quantitative indicators with a low level of quality of education, leveling the principle of the rule of the human dimension in education. Ukrainian education is facing the socio-cultural need to integrate into the European space. This path is realized through adaptation to new conditions and modernization of the educational environment using innovative strategies.O objetivo do artigo é destacar as estratégias para o desenvolvimento da educação ucraniana a curto e longo prazo do processo de integração na comunidade europeia. A tarefa da investigação científica centra-se na correlação dos processos de integração da educação ucraniana no paradigma europeu. A metodologia de investigação baseia-se numa combinação de abordagens clássicas e inovadoras do discurso científico e pedagógico. Os resultados do estudo indicam os aspectos problemáticos da transição da educação ucraniana para os padrões do sistema educativo europeu, nomeadamente, a saturação excessiva de indicadores quantitativos com um baixo nível de qualidade da educação, nivelando o princípio da regra da dimensão humana na educação. A educação ucraniana está a enfrentar a necessidade sociocultural de se integrar no espaço europeu. Esta via é concretizada através da adaptação a novas condições e da modernização do ambiente educativo, utilizando estratégias de desenvolvimento inovadoras

Dispute on the Internet: the format of discourse, linguistic technologies, modern language tactics

The concept of discourse is fundamental to modern linguistics. Discourse is a connecting link for all linguistic research, and modern ideas about discourse, without exaggeration, reflect the process of formation of linguistic science. The purpose of this work is to analyze the linguistic and stylistic features of the dispute on the Internet, namely: the format of discourse, linguistic technologies, and modern language tactics. The subject of the study is the debate on the Internet as a way to find out the positions of the participants in the discussion. During the study, a number of general scientific methods were used: the method of analogy, the method of generalization, the method of observation, the method of comparison, the method of experiment, the method of analysis, and the historical method. As a result of the research, the format of discourse, linguistic technologies, and modern language tactics of debate on the Internet were analyzed. Thus, it is emphasized that in the debate on the Internet, a combination of verbal and nonverbal components of the message realizes such an important feature of media discourse as multimedia. Non-verbal means of communication become a kind of background for the message, which thus receives additional expressive-emotional-evaluative overtones, which helps to put in the mind of the recipient a particular idea. In addition, the extreme pragmatism of modern man creates new challenges for its information. Today, to enhance the "presence effect", it is necessary to create the illusion of a new reality, an integral part of which is Internet discourse

Socio-Economic Determinants of Food Insecurity

The article presents the theoretical and methodological discourse of the concepts “food security”, “food insecurity”, “malnutrition”, “hunger” and their complementarity. The levels of food security and its range according to the USDA Food Safety Methodology are determined. The economic, social, political, climatic determinants of food insecurity are identified. The problem of prevalence of malnutrition and hunger in the world is considered. The strategic objectives of Global Food Security until 2030 are considered. The international experience of overcoming food insecurity is studied, and the effectiveness of state social programs is investigated. The importance of Ukraine’s participation in international food security programs is emphasized. The tasks and indicators of overcoming food insecurity in Ukraine by 2030 are described in accordance with the goals of sustainable development, in particular: ensuring the availability of balanced nutrition at the level of scientifically based standards for all segments of the population; increasing agricultural productivity, primarily through the use of innovative technologies; ensuring the creation of sustainable food production systems that contribute to the conservation of ecosystems and gradual improvement of soil quality; reducing food price volatility. The driving forces of food insecurity of the population and the possible ways to overcome them are determined

Long-time Behavior of State Functions for Badyko Models

In this note we examine the long-time behavior of state functions for a climate energy balance model (Budyko Model) in the strongest topologies of the phase and the extended phase spaces. Strongest convergence results for all weak solutions are obtained. New structure and regularity properties for global and trajectory attractors are justified

Суспільні фінанси України

У навчальному посібнику розкрито зміст і структуру суспільних фінансів України, особливості управління ними, способи вдосконалення міжбюджетних відносин та податкової системи країни. Для студентів, які вивчають курси «Фінанси», «Державні фінанси України», «Податкова система України», а також для аспірантів, викладачів економічних спеціальностей і фахівців фінансових установ

Business Risk in Changing Dynamics of Global Village 2

The monograph is prepared based on the presentations and discussions made at the II International Conference “BUSINESS RISK IN CHANGING DYNAMICS OF GLOBAL VILLAGE (BRCDGV 2019)”, November, 7th-08th, 2019, in Ternopil, Ukraine. The aim of this scientific international conference is to provide a platform for professional debate with the participation of experts from around the globe in order to identify & analyze risks and opportunities in today’s global business, and specifically in Ukraine. The conference will provide a framework for researchers, business elites and decision makers to uplift the business ties and minimise the risk for creating a better world and better Ukraine.The Conference is designed to call experts around the globe from different sectors of practices which are effected by globalization and watching changes in Europe as well as in Ukraine. It is an excellent platform for interactions and communication between academicians, corporate representatives, policy makers, representatives of organizations and community, as well as individuals being the part of this globalized world. The 1st edition of this conference was held at the University of Applied Sciences in Nysa, Poland (2017); the 2nd edition took place at Ternopil Ivan Puluj National Technical University, Ukraine (2019); the 3rd edition will be organized at Patna University, India (2020) in cooperation with Indo-European Education Foundation (IEEF, Poland) and its partner universities from Poland, India, Europe and other part of the world.Under modern conditions of globalization nowadays, economic activity is undergoing changes. Innovative technologies, new forms of business, dynamic changes taking place in the world today result in the emergence of the necessity to minimize risks in order to maximize benefits. The cooperation between experts from different fields with the aim to ensure sustainable growth – policymakers, scientists, universities representatives and business elites is essential nowadays. With the purpose to bring them together and discuss the main issues of todays’ global world this conference took place in Ternopil, Ukraine. As Ukraine is now passing through a dynamic period of changes, recommendations coming up from such discussions can be very beneficial for building stronger society and meet the risks globalization brings up. This monograph provides a useful review of economic, financial and policy issues in the context of globalization processes and has proven extremely popular with practitioners and industry advisors. This edition is given the continued high demand and interest for experts form different areas working on diminishing of business risks wishing to keep abreast of current thinking on this subject. According to many experts process of managing risks is currently one of the most relevant business technologies and at the same time it is a complex process which requires ground knowledge in the research field and practical experience. The popularity of business risks management is due to objective reasons such as dynamics of society, interconnections and interdependence between different players in the society, increasing role of human capital in the country’s sustainable developmen