A method to estimate trends in distributions of 1 min rain rates from numerical weather prediction data

It is known that the rain rate exceeded 0.01% of the time in the UK has experienced an increasing trend over the last 20 years. It is very likely that rain fade and outage experience a similar trend. This paper presents a globally applicable method to estimate these trends, based on the widely accepted Salonen-Poiares Baptista model. The input data are parameters easily extracted from numerical weather prediction reanalysis data. The method is verified using rain gauge data from the UK, and the predicted trend slopes of 0.01% exceeded rain rate are presented on a global grid

A cancer cell-line titration series for evaluating somatic classification.

BackgroundAccurate detection of somatic single nucleotide variants and small insertions and deletions from DNA sequencing experiments of tumour-normal pairs is a challenging task. Tumour samples are often contaminated with normal cells confounding the available evidence for the somatic variants. Furthermore, tumours are heterogeneous so sub-clonal variants are observed at reduced allele frequencies. We present here a cell-line titration series dataset that can be used to evaluate somatic variant calling pipelines with the goal of reliably calling true somatic mutations at low allele frequencies.ResultsCell-line DNA was mixed with matched normal DNA at 8 different ratios to generate samples with known tumour cellularities, and exome sequenced on Illumina HiSeq to depths of >300×. The data was processed with several different variant calling pipelines and verification experiments were performed to assay >1500 somatic variant candidates using Ion Torrent PGM as an orthogonal technology. By examining the variants called at varying cellularities and depths of coverage, we show that the best performing pipelines are able to maintain a high level of precision at any cellularity. In addition, we estimate the number of true somatic variants undetected as cellularity and coverage decrease.ConclusionsOur cell-line titration series dataset, along with the associated verification results, was effective for this evaluation and will serve as a valuable dataset for future somatic calling algorithm development. The data is available for further analysis at the European Genome-phenome Archive under accession number EGAS00001001016. Data access requires registration through the International Cancer Genome Consortium's Data Access Compliance Office (ICGC DACO)

Intense-Field Photoionization of Molecules using Ultrashort Radiation Pulses: REMPI in Toluene, Aniline, Phenol, and Fluorobenzene

We investigate the intense-field photodynamics of molecules using 50-fs pulses with intensities of 1012 to 1014 W/cm2 at 800-nm from a Ti:sapphire laser. We use a mass spectrometer that avoids focal intensity averaging. We have investigated the isoelectronic target molecules toluene (C6H5-CH3), aniline (C6H5-NH2), phenol (C6H5-OH), and fluorobenzene (C6H5-F). We discuss a resonance-enhanced multiphoton ionization (REMPI) mechanism that includes the AC Stark shifts of the molecular states. We have also investigated CO2 and CS2. For these molecules, no resonance effects are observed

A Successful Portable Computer Lab Training Program

Penn State Cooperative Extension and the Pennsylvania Farm Credit System joined forces to fund a portable computer laboratory. A simplified lab management procedure allowed Extension agents to offer 33 computer operation workshops for 300 participants at minimal participant cost. Participants indicated their future use of computers would focus on farm financial, crop, and livestock management. Although considerable competence was gained, more than 50% viewed themselves with poor to moderate computer skills at the end of the workshops. The lab has enabled agents to contact a preciously under-served population as 54% of the participants had not attended any Extension workshops in the previous year

Differential rotation measurement of soft X-Ray corona

The aim of this paper is to study the latitudinal variation in the solar rotation in soft X-ray corona. The time series bins are formed on different latitude regions of the solar full disk (SFD) images that extend from 80 degree South to 80 degree North. These SFD images are obtained with the soft X-ray telescope (SXT) on board the Yohkoh solar observatory. The autocorrelation analyses are performed with the time series that track the SXR flux modulations in the solar corona. Then for each year, extending from 1992 to 2001, we obtain the coronal sidereal rotation rate as a function of the latitude. The present analysis from SXR radiation reveals that; (i) the equatorial rotation rate of the corona is comparable to the rotation rate of the photosphere and the chromosphere, (ii) the differential profile with respect to the latitude varies throughout the period of the study; it is more in the year 1999 and least in 1994 and (iii) the equatorial rotation period varies systematically with sunspot numbers and indicates its dependence on the phases of the solar activity cycle.Comment: 9 Pages, 4 Figures, Accepted for Publication in MNRA

Finding non-eclipsing binaries through pulsational phase modulation

We present a method for finding binaries among pulsating stars that were observed by the Kepler Mission. We use entire four-year light curves to accurately mea- sure the frequencies of the strongest pulsation modes, then track the pulsation phases at those frequencies in 10-d segments. This produces a series of time-delay measurements in which binarity is apparent as a periodic modulation whose amplitude gives the projected light travel time across the orbit. Fourier analysis of this time-delay curve provides the pa- rameters of the orbit, including the period, eccentricity, angle of ascending node and time of periastron passage. Differentiating the time-delay curve yields the full radial-velocity curve directly from the Kepler photometry, without the need for spectroscopy. We show examples with delta Scuti stars having large numbers of pulsation modes, including one system in which both components of the binary are pulsating. The method is straightfor- ward to automate, thus radial velocity curves can be derived for hundreds of non-eclipsing binary stars from Kepler photometry alone. This contribution is based largely upon the work by Murphy et al. [1], describing the phase-modulation method in detail

Laboratory Focus on Improving the Culture of Biosafety: Statewide Risk Assessment of Clinical Laboratories That Process Specimens for Microbiologic Analysis

The Wisconsin State Laboratory of Hygiene challenged Wisconsin laboratories to examine their biosafety practices and improve their culture of biosafety. One hundred three clinical and public health laboratories completed a questionnaire-based, microbiology-focused biosafety risk assessment. Greater than 96% of the respondents performed activities related to specimen processing, direct microscopic examination, and rapid nonmolecular testing, while approximately 60% performed culture interpretation. Although they are important to the assessment of risk, data specific to patient occupation, symptoms, and travel history were often unavailable to the laboratory and, therefore, less contributory to a microbiology-focused biosafety risk assessment than information on the specimen source and test requisition. Over 88% of the respondents complied with more than three-quarters of the mitigation control measures listed in the survey. Facility assessment revealed that subsets of laboratories that claim biosafety level 1, 2, or 3 status did not possess all of the biosafety elements considered minimally standard for their respective classifications. Many laboratories reported being able to quickly correct the minor deficiencies identified. Task assessment identified deficiencies that trended higher within the general (not microbiology-specific) laboratory for core activities, such as packaging and shipping, direct microscopic examination, and culture modalities solely involving screens for organism growth. For traditional microbiology departments, opportunities for improvement in the cultivation and management of highly infectious agents, such as acid-fast bacilli and systemic fungi, were revealed. These results derived from a survey of a large cohort of small- and large-scale laboratories suggest the necessity for continued microbiology-based understanding of biosafety practices, vigilance toward biosafety, and enforcement of biosafety practices throughout the laboratory setting

Diffuse Gamma-Ray Emission from Starburst Galaxies and M31

We present a search for high energy gamma-ray emission from 9 nearby starburst galaxies and M31 with the EGRET instrument aboard CGRO. Though the diffuse gamma-ray emission from starburst galaxies was suspected to be detectable, we find no emission from NGC 253, M82 nor from the average of all 9 galaxies. The 2 sigma upper limit for the EGRET flux above 100 MeV for the averaged survey observations is 1.8 x 10-8 ph cm-2 s-1. From a model of the expected radio and gamma-ray emission, we find that the magnetic field in the nuclei of these galaxies is > 25 micro Gauss, and the ratio of proton and electron densities is < 400. The EGRET limits indicate that the rate of massive star formation in the survey galaxies is only about an order of magnitude higher than in the Milky Way. The upper limit to the gamma-ray flux above 100 MeV for M31 is 1.6 x 10-8 ph cm-2 s-1. At the distance of M31, the Milky Way flux would be over twice this value, indicating higher gamma-ray emissivities in our Galaxy. Therefore, since the supernova rate of the Milky Way is higher than in M31, our null detection of M31 supports the theory of the supernova origin of cosmic rays in galaxies.Comment: 17 pages, plus 1 Postscript figure, AAS Latex macros v4.0, accepted for publication in ApJ Main Journa