A decrease in photoperiod shortly after first feeding influences the development of Atlantic salmon (Salmo salar)

Four groups of Atlantic salmon fry (n=2000) were exposed to continuous light (LD24:0) from first feeding on 18th April 2001, after which they were exposed to either an 8 or 12 week period of short days (LD10:14) starting on either the 21st May or the 18th June. Each group was then returned to LD24:0 until the conclusion of the experiment the following March. In August 200 fish per treatment were individually PIT tagged. All groups were maintained under an ambient temperature regime. The highest levels of sexual maturation in 0+ male parr were recorded in the 12 week/May group (>11% of the entire male and female population), with the lowest levels (6%) in the 8 week/May and 8 week/June groups (

Biophysical Regulation of Chromatin Architecture Instills a Mechanical Memory in Mesenchymal Stem Cells

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material.This work was supported by the National Institutes of Health (R01 AR056624, R01 EB02425, T32 AR007132, and P30 AR050950) and a Marie Curie Intra European Fellowship (GENOMICDIFF, grant No. 301509). Additional support was provided by a Montague Research Award from the Perelman School of Medicine and a University of Pennsylvania University Research Foundation Award

The Size of the Radio-Emitting Region in Low-luminosity Active Galactic Nuclei

We have used the VLA to study radio variability among a sample of 18 low luminosity active galactic nuclei (LLAGNs), on time scales of a few hours to 10 days. The goal was to measure or limit the sizes of the LLAGN radio-emitting regions, in order to use the size measurements as input to models of the radio emission mechanisms in LLAGNs. We detect variability on typical time scales of a few days, at a confidence level of 99%, in half of the target galaxies. Either variability that is intrinsic to the radio emitting regions, or that is caused by scintillation in the Galactic interstellar medium, is consistent with the data. For either interpretation, the brightness temperature of the emission is below the inverse-Compton limit for all of our LLAGNs, and has a mean value of about 1E10 K. The variability measurements plus VLBI upper limits imply that the typical angular size of the LLAGN radio cores at 8.5 GHz is 0.2 milliarcseconds, plus or minus a factor of two. The ~ 1E10 K brightness temperature strongly suggests that a population of high-energy nonthermal electrons must be present, in addition to a hypothesized thermal population in an accretion flow, in order to produce the observed radio emission.Comment: 61 pages, 17 figures, 10 tables. Accepted for publication in the Astrophysical Journa

Comparative studies of the preparation of immunoliposomes with the use of two bifunctional coupling agents and investigation of in vitro immunoliposome-target cell binding by cytofluorometry and electron microscopy

The two coupling agents SPDP (N-succinimidyl-3-(2-pyridyldithio)propionate) and SATA (N-succinimidyl-S-acetylthioacetate) were compared in their efficiency and feasibility to couple monoclonal antibodies (Abs) via thioether linkage to liposomes functionalized by various lipophilic maleimide compounds like Full-size image methyl ester (MP-PL), N-(3-maleimidopropionyl)phosphatidylethanolamide (MP-PE), Full-size image methyl ester (EMC-PL), and N-(6-maleimidocaproyl)phosphatidylethanolamine (EMC-PE). The composition of the liposomes was soy phosphatidylcholine (SPC), cholesterol, maleimide compounds and -tocopherol (1:0.2:0.02:0.01, mol parts), plus N4-oleylcytosine arabinoside (NOAC) as cytostatic prodrug (0.2 mol parts) and a new, lipophilic and highly fluorescent dye N,N′-bis(1-hexylheptyl)-3,4:9,10-perylenebis(dicarboximide) (BHPD, 0.006 mol parts). From the maleimide derivatives MP-PL was the most effective in terms of preservation of the coupling activity in dependence of liposome storage. The coupling of the monoclonal A B8-24.3 (mouse IgG2b, MHC class I, anti H-2kb) and IB16-6 (rat IgG2a, anti B16 mouse melanoma) to the drug carrying liposomes was more effective and easier to accomplish with SATA as compared to SPDP. Coupling rates of 60–65% were obtained with SATA at molar ratios of 12 SATA:1 Ab:40 maleimide spacer groups on the surface of one liposome. The highest coupling rates with SPDP were obtained at the ratio of 24 SPDP:1 Ab:40 liposomal maleimide groups, with an Ab binding efficiency of only 20–25%. The optimal in vitro binding conditions to specific target cells (EL4 for B8-24.3-liposomes and B16-F10 for IB16-6-liposomes) were determined by cytofluorometric measurement of the liposomal BHPD fluorescence with SATA linked Abs. Optimal immunoliposome binding to specific epitopes on the target cells was achieved with 1–2 Ab molecules coupled to one liposome, with immunoliposome concentrations of 20–130 nM and with a small incubation volume of 0.3–0.4 ml. The specificity of the binding of B8-24.3-liposomes to EL4 target cells was visualized by scanning electron microscopy. Antibody mediated endocytic uptake of immunoliposomes could be demonstrated by transmission electron microscopy