El estrés laboral y el rendimiento de los servidores públicos de las areas administrativas en el programa Integral Nacional de Bienestar Familiar - INABIF de Lima, 2021

El presente trabajo de investigación tuvo como objetivo determinar lainfluencia del estrés laboral en el rendimiento de los servidores públicos de las áreas administrativas en el Programa Integral Nacional de BienestarFamiliar - INABIF, lima, 2021. El método empleado fue de tipo aplicada, diseño no experimental de corte transversal, nivel descriptivo correlacional de enfoque cuantitativo. La muestra probabilística estuvo conformada por 30 trabajadores, utilizando como técnica la encuesta y como instrumento el cuestionario. Los datos fueron procesadosestadísticamente en el programa SPSS versión 24. Los resultados muestran que del 100% de encuestados el el 6.7% de los servidores públicos del área de Potencial Humano del INABIF perciben que el estrés laboral es regular, mientras el 46.7% señala que es alto y el 46.7% muy alto. Respecto al rendimiento, el 43,3% de los servidores públicos del área de Potencial Humano, perciben que el rendimiento laboral es bueno y el 56,7% de ellos indican excelente.La prueba Chi Cuadrado dio como resultado P−valor=0,072 > 0.05, con nivel de significación α = 0.05, por lo cual, se rechazó la hipótesis alterna Ha y se acepta la hipótesis nula Ho. Se concluye que el estrés laboral no se relaciona significativamente en el rendimiento de los servidores públicos delas áreas administrativas en el Programa Integral Nacional de BienestarFamiliar - INABIF, lima, 202

Isolation and characterization of a dideoxyguanosine triphosphate-resistant mutant of human immunodeficiency virus reverse transcriptase

The appearance of drug-resistant strains of viral pathogens is a major difficulty confounding current efforts to block viral infections. The identification and analysis of mutations responsible for drug resistance can provide important clues helpful in understanding the mechanisms of resistance and in the eventual development of better therapies. We have used a direct screening method to scan libraries of mutagenized genes encoding the reverse transcriptase of human immunodeficiency virus type 1, and have recovered a variant enzyme that is resistant to the chain-terminator inhibitor 2',3'-dideoxyguanosine triphosphate. The single substitution mutation in this variant conferred broad crossresistance to a variety of other antiviral compounds currently in clinical trials. Virus carrying the mutation was fully infectious in cultured human lymphocytes. The replication of the mutant virus was highly resistant to phosphonoformic acid but did not show increased resistance to the prodrug dideoxyguanosine

Editorial : Foot-and-mouth disease epidemiology, vaccines and vaccination : moving forward

Vaccination has played a major role in foot-and-mouth disease (FMD) control. There are different approaches to the design and implementation of vaccination campaigns, and epidemiological information is paramount in influencing the vaccine and vaccination strategy that best suit each geographic location. FMD-endemic regions typically organize vaccination campaigns as a routine preventive control policy or to mitigate the impact of the disease. The majority of currently used vaccines are formulated with chemically inactivated whole-viral particles and suitable adjuvants such as single and double oil emulsions. The most recent strains circulating in a particular region are typically selected as antigens based on the results of vaccine-matching data and in vitro experiments, however, predictions based on vaccine-matching approaches are usually uncertain without a live virus challenge in natural hosts combined with reliable field data. Vaccine selection and successful vaccination campaigns rely on a deep knowledge of the epidemiology of the region where these vaccines will be used, as well as access to the appropriate diagnostic tools to underpin these campaigns.Instituto de VirologíaFil: Capozzo, Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; ArgentinaFil: Capozzo, Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vosloo, Wilna. Commonwealth Scientific and Industrial Research Organisation (CSIRO) Health and Biosecurity. Transboundary Disease Mitigation. Australian Centre for Disease Preparedness; AustraliaFil: de los Santos, Teresa. United States Department of Agriculture. Agricultural Research Service. Plum Island Animal Disease Center; Estados UnidosFil: Pérez, Andrés M. University of Minnesota. Department of Veterinary Population Medicine; Estados UnidosFil: Perez Filgueira, Daniel Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; ArgentinaFil: Perez Filgueira, Daniel Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Cultural Heritage Restoration of a Hemispherical Vault by 3D Modelling and Projection of Video Images with Unknown Parameters and from Unknown Locations

[EN] Reverse engineering applied to architectural restoration for the reconstruction of structural surfaces depends on metric precision. Sometimes there are elements on these surfaces whose value is even higher than the building itself. This is the case for many churches whose ceilings have pictorial works of art. Reconstruction requires the existence of some identifiable remainder and/or a surface geometry that enables mathematical development. In our case, the vault has an irregular hemispherical geometry (without possible mathematical development), and there are no significant remains of the painting (which was destroyed by a fire). Through the 3D modelling of the irregular vault and two historic frames with a camera of unknown geometry, an inverse methodology is designed to project the original painting without metric deformations. For this, a new methodology to locate the camera positions is developed. After, a 3D virtual mathematical model of the complete image on the vault is calculated, and from it, partial 3D virtual images are automatically calculated depending on the variable unknown positions of the video cannons (distributed along the upper corridor of the apse) that will project them (visually forming a perfect complete 3D image)Herráez Boquera, J.; Denia Rios, JL.; Priego De Los Santos, E.; Navarro Esteve, PJ.; Martín Sánchez, MT.; Rodríguez Pereña, J. (2021). Cultural Heritage Restoration of a Hemispherical Vault by 3D Modelling and Projection of Video Images with Unknown Parameters and from Unknown Locations. Applied Sciences. 11(12):1-12. https://doi.org/10.3390/app11125323112111

Venezuelan Equine Encephalitis Replicon Particles Can Induce Rapid Protection against Foot-and-Mouth Disease Virus

We have previously shown that delivery of the porcine type I interferon gene (poIFN-α/β) with a replication-defective human adenovirus vector (adenovirus 5 [Ad5]) can sterilely protect swine challenged with foot-and-mouth disease virus (FMDV) 1 day later. However, the need of relatively high doses of Ad5 limits the applicability of such a control strategy in the livestock industry. Venezuelan equine encephalitis virus (VEE) empty replicon particles (VRPs) can induce rapid protection of mice against either homologous or, in some cases, heterologous virus challenge. As an alternative approach to induce rapid protection against FMDV, we have examined the ability of VRPs containing either the gene for green fluorescent protein (VRP-GFP) or poIFN-α (VRP-poIFN- α) to block FMDV replication in vitro and in vivo. Pretreatment of swine or bovine cell lines with either VRP significantly inhibited subsequent infection with FMDV as early as 6 h after treatment and for at least 120 h posttreatment. Furthermore, mice pretreated with either 107 or 108 infectious units of VRP-GFP and challenged with a lethal dose of FMDV 24 h later were protected from death. Protection was induced as early as 6 h after treatment and lasted for at least 48 h and correlated with induction of an antiviral response and production of IFN- α. By 6 h after treatment several genes were upregulated, and the number of genes and the level of induction increased at 24 h. Finally, we demonstrated that the chemokine IP-10, which is induced by IFN- α and VRP-GFP, is directly involved in protection against FMDV

Expression of Porcine Fusion Protein IRF7/3(5D) Efficiently Controls Foot-and-Mouth Disease Virus Replication

Several studies have demonstrated that the delivery of type I, II, or III interferons (IFNs) by inoculation of a replication-defective human adenovirus 5 (Ad5) vector expressing IFNs can effectively control foot-and-mouth disease (FMD) in cattle and swine during experimental infections. However, relatively high doses are required to achieve protection. In this study, we identified the functional properties of a porcine fusion protein, poIRF7/3(5D), as a biotherapeutic and enhancer of IFN activity against FMD virus (FMDV). We showed that poIRF7/3(5D) is a potent inducer of type I IFNs, including alpha IFN (IFN-α), IFN-β, and IFN-ω but not type III IFN (interleukin-28B), without inducing cytotoxicity. Expression of poIRF7/3(5D) significantly and steadily reduced FMDV titers by up to 6 log(10) units in swine and bovine cell lines. Treatment with an IFN receptor inhibitor (B18R) combined with an anti-IFN-α antibody neutralized the antiviral activity in the supernatants of cells transduced with an Ad5 vector expressing poIRF7/3(5D) [Ad5-poIRF7/3(5D)]. However, several transcripts with known antiviral function, including type I IFNs, were still highly upregulated (range of increase, 8-fold to over 500-fold) by poIRF7/3(5D) in the presence of B18R. Furthermore, the sera of mice treated with Ad5-poIRF7/3(5D) showed antiviral activity that was associated with the induction of high levels of IFN-α and resulted in complete protection against FMDV challenge at 6, 24, or 48 h posttreatment. This study highlights for the first time the antiviral potential of Ad5-poIRF7/3(5D) in vitro and in vivo against FMDV. IMPORTANCE FMD remains one of the most devastating diseases that affect livestock worldwide. Effective vaccine formulations are available but are serotype specific and require approximately 7 days before they are able to elicit protective immunity. We have shown that vector-delivered IFN is an option to protect animals against many FMDV serotypes as soon as 24 h and for about 4 days postadministration. Here we demonstrate that delivery of a constitutively active transcription factor that induces the production of endogenous IFNs and potentially other antiviral genes is a viable strategy to protect against FMD

Considering and interpreting leisure: pastimes, entertainments, hobbies and addictions in the Barcelona of 1900

Coordinació: Teresa-M. SalaIn the throes of modernization, the Barcelona of 1900 was buzzing with energy and an unprecedented culture of leisure emerged. The city’s residents made full use of their free time, forging relationships with one another through a highly varied range of initiatives and activities, taking advantage of existing opportunities and creating new ones. In addition to the traditional celebration of civic festivals, popular meals and various types of processions, innovative new forms of cultural consumption sprang up to satisfy all tastes and budgets. Numerous cinemas opened, the first amusement parks threw open their gates and new theatres raised their curtains. Thanks to these developments, the vicinity around the avenue Paral·lel became the epicentre of Barcelona nightlife. This book offers a panoply of images of the diversions, entertainments and hobbies of that fascinating era of contrasts, the turn-of-the-century Barcelona of Modernisme

Creación del primer Grupo iGEM (Competición Internacional de Biología Sintética) de Madrid: Facultad de Biología-UCM

En este Trabajo se describe la creación del primer Grupo iGEM de Madrid así como la elaboración de un biosensor que permite la detección de polen de olivo a tiempo real empleando metodos de ingeniería genética y robótica