586 research outputs found

    Multisite Evaluation of Cepheid Xpert Carba-R Assay for Detection of Carbapenemase-Producing Organisms in Rectal Swabs.

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    Rapid identification of patients who are colonized with carbapenemase-producing organisms (CPO) is included in multiple national guidelines for containment of these organisms. In a multisite study, we evaluated the performance of the Cepheid Xpert Carba-R assay, a qualitative diagnostic test that was designed for the rapid detection and differentiation of the blaKPC, blaNDM, blaVIM, blaOXA-48, and blaIMP-1 genes from rectal swab specimens. A double rectal swab set was collected from 383 patients admitted at four institutions (2 in the United States, 1 in the United Kingdom, 1 in Spain). One swab was used for reference culture (MacConkey broth containing 1 mg/liter of meropenem and subcultured to a MacConkey agar plate with a 10-μg meropenem disk) and for sequencing of DNA obtained from carbapenem-nonsusceptible isolates for carbapenemase identification. The other swab was used for the Xpert Carba-R assay. In addition to the clinical rectal swabs, 250 contrived specimens (108 well-characterized CPO and 142 negative controls spiked onto negative rectal swabs) were tested. Overall, 149/633 (23.5%) samples were positive by the Xpert Carba-R assay. In 6 samples, multiple targets were detected (4 VIM/OXA-48, 1 IMP-1/NDM, and 1 NDM/KPC). The Xpert Carba-R assay detected 155 targets (26 IMP-1, 30 VIM, 27 NDM, 33 KPC, 39 OXA-48) within a time range of 32 to 48 min. The sensitivity, specificity, and positive and negative predictive values of the Xpert Carba-R assay compared to those of the reference culture and sequencing results were 96.6% (95% confidence interval [CI], 92.2% to 98.9%), 98.6% (95% CI, 97.1% to 99.4%), 95.3%, and 99.0%, respectively. The Cepheid Xpert Carba-R assay is an accurate and rapid test to identify rectal colonization with CPO, which can guide infection control programs to limit the spread of these organisms

    Desarrollo de nuevas soluciones digitales para clientes en el ámbito de las water agencies

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    Comunicación presentada al XXXVII Congreso Nacional de Riegos, celebrado en Don Benito del 4 al 6 de Junio de 2019 y organizada por la Asociación Española de Riegos y Drenajes y la Universidad de ExtremaduraEn escenarios en los que la escasez de agua y la necesidad de optimización energética cobran vital importancia, las soluciones tecnológicas se presentan como herramienta que proporciona conocimiento y asiste a la explotación del recurso de forma eficiente e inteligente. Los avances tecnológicos permiten el desarrollo de soluciones basadas en software y hardware abierto, lo que redunda en soluciones de alta competitividad, flexibles y de bajo coste.In a lack of water and special needs for power optimization stages, technological solutions are presented as tools witch provide knowledgement and attends resource operation in an efficient and intelligent way. Technological advances allows the development of solutions based in open hardware and software, what leads in high competitivity, flexible and cost effective solutions

    Pt(II) coordination complexes as visible light photocatalysts for the oxidation of sulfides using batch and flow processes

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    A new catalytic system for the photooxidation of sulfides based on Pt(ii) complexes is presented. The catalyst is capable of oxidizing a large number of sulfides containing aryl, alkyl, allyl, benzyl, as well as more complex structures such as heterocycles and methionine amino acid, with complete chemoselectivity. In addition, the first sulfur oxidation in a continuous flow process has been developedJ. A. would also like to thank the MICINN for their ‘Ramón y Cajal’ contract and the European Research Council (ERC-CG, contract number 647550

    IMPACT OF PASSIVE LEG MOVEMENT ON LOWER LIMB VASCULAR FUNCTION IN PATIENTS WITH A SPINAL CORD INJURY

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    Background: Individuals with spinal cord injuries (SCI) are at a greater risk for developing cardiovascular diseases. Of note, post injury mediated increases in physical inactivity leads to muscle atrophy, which also results in vascular dysfunction in this population. Although a growing body of evidence suggests that passive leg movement (PLM) may be a useful exercise modality to improve peripheral blood flow and skeletal muscle activation in individuals with limited mobility, few studies have examined the impact of PLM on local skeletal muscle blood flow. Therefore, there is a need to examine the impact of PLM on lower limb vascular function and skeletal muscle oxygen utilization capacity in patients with SCI. Methods: Individuals with SCI (n=2) and healthy age-matched controls (CON, n=5) were recruited for this study. Participants were fitted with a standard knee brace and were instructed to rest in the seated position for 20 minutes with their legs bent at 90. PLM was performed by flexion and extension of the lower leg (90-180) at a rate of 1 Hz (60 bpm) for 5 minutes. A Doppler ultrasound was located on the superficial femoral artery, and blood flow and diameter were measured for 5 minutes at rest and during the PLM protocol. Following the PLM protocol, the leg was held in the extended position (180) for 5 minutes of recovery. Results: We found that the SCI group had a significantly lower blood flow response to PLM compared to the CON group (p=0.004). Furthermore, post-PLM femoral artery blood velocity and shear rate significantly increased in both SCI and CON compared to pre-PLM (p=0.014, and p=0.016, respectively) but no differences were found between groups. Finally, the SCI group had significantly smaller vessel diameters compared with CON (pConclusion: We found that PLM could efficiently increase blood flow and blood velocity in SCI. Although the magnitude of this increase was significantly lower in SCI compared to healthy age-matched control, PLM produced shear rates in the leg arteries that were similar between SCI and CON, which indicates that PLM may potentially be an efficient exercise modality to improve leg vascular function in individuals with SCI

    Triacylglyceride, antioxidant and antimicrobial features of virgin camellia oleifera, C. reticulata and C. sasanqua oils

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    Virgin oils obtained from seeds of Camellia oleifera (CO), Camellia reticulata (CR) and Camellia sasanqua (CS) were studied for their triacylglyceride composition, antioxidant and antimicrobial activities. Levels of fatty acids determined by 1H-nuclear magnetic resonance analysis were similar to those reported for olive oils (82.30%–84.47%; 5.69%–7.78%; 0.26%–0.41% and 8.04%–11.2%, for oleic, linoleic, linolenic and saturated acids, respectively). The CR oil showed the best antioxidant potential in the three in vitro models tested. With regard to EC50 values (μg/mL), the order in DPPH radical-scavenging was CR (33.48) < CO (35.20) < CS (54.87). Effectiveness in reducing power was CR (2.81) < CO (3.09) < CS (5.32). IC50 for LPO inhibition were 0.37, 0.52 and 0.75 μg/mL for CR, CO and CS, respectively. All the oils showed antimicrobial activity, and exhibited different selectivity and MICs for each microorganism tested (E. coli, B. cereus and C. albicans). B. cereus was the less sensitive species (MIC: 52.083 ± 18.042 for CO; 41.667 ± 18.042 for CR; 104.167 ± 36.084 for CS mg/mL) and the E. coli was the most sensitive to camellia oil’s effect. The standard gentamicin presented higher MIC for E. coli (4.2) than the CR (MIC= 2.6) and CO (MIC = 3.9) oils

    Evolutionary-based prediction interval estimation by blending solar radiation forecasting models using meteorological weather types

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    Recent research has shown that the integration or blending of different forecasting models is able to improve the predictions of solar radiation. However, most works perform model blending to improve point forecasts, but the integration of forecasting models to improve probabilistic forecasting has not received much attention. In this work the estimation of prediction intervals for the integration of four Global Horizontal Irradiance (GHI) forecasting models (Smart Persistence, WRF-solar, CIADcast, and Satellite) is addressed. Several short-term forecasting horizons, up to one hour ahead, have been analyzed. Within this context, one of the aims of the article is to study whether knowledge about the synoptic weather conditions, which are related to the stability of weather, might help to reduce the uncertainty represented by prediction intervals. In order to deal with this issue, information about which weather type is present at the time of prediction, has been used by the blending model. Four weather types have been considered. A multi-objective variant of the Lower Upper Bound Estimation approach has been used in this work for prediction interval estimation and compared with two baseline methods: Quantile Regression (QR) and Gradient Boosting (GBR). An exhaustive experimental validation has been carried out, using data registered at Seville in the Southern Iberian Peninsula. Results show that, in general, using weather type information reduces uncertainty of prediction intervals, according to all performance metrics used. More specifically, and with respect to one of the metrics (the ratio between interval coverage and width), for high-coverage (0.90, 0.95) prediction intervals, using weather type enhances the ratio of the multi-objective approach by 2%¿. Also, comparing the multi-objective approach versus the two baselines for high-coverage intervals, the improvement is 11%¿% over QR and 10%¿% over GBR. Improvements for low-coverage intervals (0.85) are smaller.The authors are supported by projects funded by Agencia Estatal de Investigación, Spain (PID2019-107455RB-C21 and PID2019-107455RB-C22/AEI/10.13039/501100011033). Also supported by Spanish Ministry of Economy and Competitiveness, project ENE2014-56126-C2-1-R and ENE2014-56126-C2-2-R (http://prosol.uc3m.es). The University of Jaén team is also supported by FEDER, Spain funds and by the Junta de Andalucía, Spain (Research group TEP-220

    Methodology of integrated evaluation of environmental and occupational risks

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    Este trabalho insere-se numa óptica de integração dos Sistemas de Gestão. Apresenta uma metodologiaque procura enquadrar a avaliação de riscos das componentes ambiental e ocupacional, no sentido depotenciar sinergias que propiciem a melhoria do desempenho das organizações nestes domínios. Nestesentido, pretende-se contribuir para uma maior integração dos sistemas de gestão ao nível do planeamentodo sistema. É proposto neste artigo uma metodologia simples que visa a determinação simultânea dosimpactes ambientais e os riscos relacionados com a Segurança, Higiene e Saúde Ocupacionais,pretendendo ser um elemento facilitador no tratamento destas temáticas no seio das organizações, emparticular PME.The present paper falls within the approach to management systems integration. A methodology is presented, aiming to frame both environmental and occupational components in risk assessment, potentiating synergies to propitiate improvement in organizational performance in these issues. In this context, this study is intended as a contribution to a greater integration of management systems at planning stage. In the present article, a simple method is proposed to target the simultaneous determination of environmental impacts and risks related to Occupational Safety and Health, thus trying to positioning as a facilitator tool in approaching these matters within organizations, specially small and medium sized enterprises, SMEs

    Antimicrobial properties of Camellia oleifera oil

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    Camellias have been used in oriental ethnomedicine and appear very promising for pharmaceutical exploitation since modern science has made it possible to specify their potential medical significance with antimicrobial, antioxidant, antiallergic, antiviral and skin healing properties. In the present study oil obtained from seeds of Camellia oleifera was studied for its antimicrobial activity using clinically isolated bacterial and yeast strains. The oil evidenced antimicrobial activity, and showed different selectivity and MICs for each microorganism tested. Obtained results indicate that the tested oil acted best in relation to Gram (-) bacteria than Gram (+). The data obtained in the in vitro models clearly establish its antimicrobial potency.XUNTA DE GALICIA for financial support: Grants INCITE09 262346PR and PGIDIT06RAG26103PR. X.F. would also like to thank the Xunta de Galicia (Isidro Parga Pondal Program for young researchers, Grant No. IPP-020
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