Marketing relacional y la calidad de servicio a los pacientes del Centro Médico Dr. Arqueño, Huánuco 2022

La presente investigación tuvo como objetivo principal describir la influencia del marketing relacional en la calidad del servicio a los pacientes del centro médico Dr. Arqueño, Huánuco 2022. El estudio fue desarrollado a través de una metodología de tipo básica, con un enfoque cuantitativo, de nivel descriptivo - correlacional y con un diseño no experimental. La población estuvo conformada por 168 pacientes del Centro médico Dr Arqueño, obteniendo una muestra de estudio de 117 pacientes, los cuales, por medio de la técnica de estudio, la encuesta; fueron encuestados por un cuestionario compuesto por 19 ítems, siendo este el instrumento de la investigación. Luego de la recogida de información y su procesamiento respectivo, la investigación concluye en la existencia de relación significativa entre el marketing relacional y la calidad de servicio del Centro Médico Dr. Arqueño, Huánuco 2022, la cual se sostiene en valores estadísticos derivados del coeficiente de correlación se Spearman, cuyo valor arrojado es de 0,793 correspondiente a una relación positiva alta; en ese sentido podemos afirmar que a mayor marketing relacional mejora la calidad del servicio del Centro Médico Dr. Arqueño

El uso de las estrategias metacognitivas en la enseñanza del inglés

Ciertas consideraciones (Brown,1990) son necesarias para desarrollar una buena Comprensión Oral (CO en adelante). La CO se puede ver afectada cuando: - Se desconoce el contexto real; - El contenido gramatical tiende a prevalecer en las clases; - El interés principal es abordar contenidos temáticos por medio de las habilidades; - No se reconoce ni se da la debida atención a la diversidad presente en el aula; y, - Cuando no se promueven de manera explícita las estrategias de aprendizaje, específicamente las metacognitivas. El objetivo de la presente investigación es diagnosticar la ocurrencia del uso de las estrategias metacognitivas en la CO en las clases de English as a Foreign Language (EFL) del grado noveno de un colegio privado mixto, por lo tanto, se abordarán los conceptos de Aprendizaje de Lengua Extranjera, Compresión Oral, y Estrategias de Aprendizaje (Oxford,1990) específicamente las metacognitivas. Usando la etnografía del aula como metodología de indagación y utilizando la observación y el diario de campo como las principales herramientas de recolección de datos de la presente investigación, se identificaron las estrategias metacognitivas que se promovían en la práctica de la CO en el aula, en qué momentos de la práctica de la CO se usaron y finalmente se examinó la forma en la que se promovieron. Se encontró que, aunque se promueven las estrategias metacognitivas, esto no sucede de manera uniforme con todas; se promueven más en algunos momentos de la CO que en otros; y además se promueven de maneras diversas. En términos generales, pareciera necesario revisar el rol que se le asigna a la CO, y el papel que se le da a las estrategias de aprendizaje metacognitivas dentro del aprendizaje de la lengua extranjera (L2).Certain considerations (Brown,1990) are necessary to develop a good Listening, which may be affected first of all, when the real context seems not to be used at the moment of addressing it; secondly, when the grammatical content tends to prevail in the classes; thirdly, when the main interest is to address topics through the skills, when the diversity present in the classroom seems neither to be recognized nor to give it attention; finally, when learning strategies, specifically the metacognitive, do not seem to be promoted explicitly. Since the aim of the present research is to diagnose the occurrence of the use of metacognitive strategies in EFL's Listening in ninth grade classes of a co-educational private school, concepts like Learning a Foreign Language, Listening, and Learning Strategies (Oxford, 1990), specifically the metacognitive, are addressed. Using the ethnography of the classroom as a methodology of research and using the observation and the field diary as the principal tools to collect information in this research, the metacognitive strategies that were promoted in the practice of the Listening in the classroom were identified, then in which moments of the practice of the Listening they were used, and finally the way they were promoted. It was found that, although the metacognitive strategies are promoted at the school, this does not happen in a uniform way with all of them; they are promoted more in some moments of the Listening than in others; and they are promoted in different ways. In general terms, it seems to be necessary to check the role that is assigned to the Listening and the role that is assigned to the metacognitive learning strategies inside the learning of L2.Licenciado (a) en Lenguas ModernasPregrad

A systematic review of the ecological and longitudinal methods to study daily stress

The objective was to review the methods used to assess daily stress, focusing on the types records used, as well as the methods used to describe daily stressors, the ways to operationally define stress, and the different research approaches. A search for quantitative research articles published between January 2008 and December 2017 was carried out on indexed entries of four electronic databases. Of the 254 publications found in the search after duplicates were removed, eft 57 articles were selected to analyse. A large diversity of recording methods was detected, a single daily record for a week being the most frequently used. The different ways to operationalize stress highlight the different implicit definitions of stress: the number or intensity of stressful event refers to stress as an external factor, negative feelings refer to the individual's responses, and reactivity or "pile-up" are related to the process by which stress develops over time. Such variation suggests that stress is not a precise concept that can be assessed by a single measure, stress is rather a generic label for the complex process of adaptation to specific situations. The first one is that it can be concluded that stress is a process that explains the short- and long-term effects of exposure to stressors on health and wellbeing through a complex chain of mediators and moderators. The second point is that although it is known that the changes produced in stressful situations are adaptive at first, studies of the negative side of stress prevail. And the third point is that the studies analysed were not reduced to the analysis of the stress process or of any particular aspect of stress but rather, the evaluation of daily stress served to study other processes with marked social and affective components

Decay-Accelerating Factor 1 Deficiency Exacerbates Leptospiral-Induced Murine Chronic Nephritis and Renal Fibrosis

Leptospirosis is a global zoonosis caused by pathogenicLeptospira, which can colonize the proximal renal tubules andpersist for long periods in the kidneys of infected hosts. Here, we characterized the infection of C57BL/6J wild-type andDaf12/2mice, which have an enhanced host response, with a virulentLeptospira interrogansstrain at 14 days post-infection,its persistence in the kidney, and its link to kidney fibrosis at 90 days post-infection. We found thatLeptospira interroganscan induce acute moderate nephritis in wild-type mice and is able to persist in some animals, inducing fibrosis in theabsence of mortality. In contrast, Daf12/2mice showed acute mortality, with a higher bacterial burden. At the chronic stage,Daf12/2mice showed greater inflammation and fibrosis than at 14 days post-infection and higher levels at all times thanthe wild-type counterpart. Compared with uninfected mice, infected wild-type mice showed higher levels of IL-4, IL-10 andIL-13, with similar levels ofa-smooth muscle actin, galectin-3, TGF-b1, IL-17, IFN-c, and lower IL-12 levels at 90 days post-infection. In contrast, fibrosis in Daf12/2mice was accompanied by high expression ofa-smooth muscle actin, galectin-3, IL-10, IL-13, and IFN-c, similar levels of TGF-b1, IL-12, and IL-17 and lower IL-4 levels. This study demonstrates the link betweenLeptospira-induced murine chronic nephritis with renal fibrosis and shows a protective role of Daf1.Fil: Ferrer, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Scharrig Fernandez, Maria Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Alberdi, Maria Lucrecia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Cédola, Maia Tatiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Prêtre, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Drut, Ricardo. Universidad Nacional de la Plata. Facultad de Cs.médicas. Cátedra de Patología Ii; ArgentinaFil: Song, Wen-Chao. University of Pennsylvania; Estados UnidosFil: Gomez, Ricardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentin

Tv comedy in Spain: the transition fiction between 1990 -1995

El trabajo analiza los estándares y características de la comedia televisiva española, mediante un estudio cualitativo sobre 30 comedias emitidas desde la desregularización televisiva y durante los cinco años posteriores (1990-1995). Entendiendo este lustro como la transición de la programación televisiva en el nuevo escenario de confluencia pública y privada, el objetivo es conocer la determinación de estos estándares primitivos que cimentaron el desarrollo de la comedia en televisión.This work analyzes the standards and characteristics of the Spanish TV comedy, through a qualitative study of 30 comedies issued since TV deregulation and the next five years (1990-1995). Understanding this lustrum as the transition of TV programming in the new scene of public and private confluence, the objective is to know the determination of these primitive standards that settled the development of TV comedy.Este trabajo se ha realizado dentro del proyecto de investigación: “Historia de la programación y programas de ficción televisiva en España (cadenas de ámbito estatal): De la Desregulación al Apagón analógico, 1990-2010” financiado por el Ministerio de Economía y Competitividad

Effect of alternating anaerobic and aerobic phases on the performance of a SBR treating effluents with high salinity and phenols concentration

The biological treatment of hypersaline wastewaters with high COD and phenols concentration requires long hydraulic retention times and much energy for aeration. In this work aeration time reduction in the reaction phase was studied in two sequential batch reactors (SBR) treating fermentation brine from table olive processing wastewater. To study the influence of an anaerobic phase on the SBR performance (for COD and phenolic compound removal efficiencies), different anaerobic/aerobic reaction times were evaluated. SBR-1 was operated with an anaerobic/aerobic hours ratio of 0/22, 8/14 and 14/8 and SBR-2 with a ratio of 22/0, 14/8 and 8/14. Results showed that the maximum organic matter reduction was obtained under aerobic reaction conditions (ratio 0/22) with a 82.3% and 77.9% of COD and total phenols removal, respectively. However, optimal conditions were considered to prevail for an anaerobic/aerobic ratio of 8/14, since the reactors performances were similar with lower energy consumption. Thus, 82.3% and 79.5% of COD and 77.9% and 78.3% of total phenols were removed in SBR-1 and SBR-2, respectively.The authors of this work thank the financial support of CDTI (Centre for Industrial Technological Development) depending on the Spanish Ministry of Science and InnovationFerrer-Polonio, E.; Garcia Quijano, NT.; Mendoza Roca, JA.; Iborra Clar, A.; Pastor Alcañiz, L. (2016). Effect of alternating anaerobic and aerobic phases on the performance of a SBR treating effluents with high salinity and phenols concentration. Biochemical Engineering Journal. 113:57-65. doi:10.1016/j.bej.2016.05.010S576511

Análisis de la diversidad genética de las regiones HVI y HVII del genoma mitocondrial en una muestra de la población de Maracaibo, Venezuela | Genetic diversity of HVI and HVII mitocondrial genome regions in a population sample of Maracaibo, Zulia state, Venezuela

El estudio de los polimorfismos de las regiones hipervariables I y II del ADN mitocondrial se ha convertido en una herramienta invaluable para la ciencia forense, permitiendo el análisis de material biológico exiguo, así como la filiación biológica por línea materna. Con el objetivo de analizar la diversidad genética de las regiones HVI y HVII del genoma mitocondrial en la población de Maracaibo, estado Zulia, se eligieron 50 individuos, cuyos ADN se amplificaron mediante la reacción en cadena de la polimerasa, se secuenciaron las regiones hipervariables mediante método de Sanger y los fragmentos se separaron por electroforesis capilar y fueron reportadas las diferencias con respecto a la secuencia de referencia de Cambridge. La diversidad genética de ambas regiones fue de 0,9837 ± 0,0084, la diversidad nucleotídica de 0,020001 ± 0,010201, la media de las diferencias por parejas 12,160816 ± 5,588550, el poder de discriminación 0,9640 y la probabilidad de coincidencia al azar 0,0360. Estos resultados determinaron que los haplogrupos observados en las muestras analizadas correspondieran a Amerindios 68% (A 32% B 18%, C 4% y D 14%), Africanos 20% (L 20%), Europeos 8 % (H 2%, K 2% U 2%, W 2%) y Asiáticos 2% (N 2%). Los datos demuestran que los polimorfismos en las regiones HVI y HVII son suficientemente informativos como para ser utilizados en la casuística forense. Palabras clave: ADN mitocondrial, regiones hipervariables, región control, identificación humana, población venezolana.  ABSTRACT The study of the polymorphisms of hypervariable regions I and II of mitochondrial DNA; has become an invaluable tool for forensic science due to features like its exclusively maternal inheritance; allowing the analysis of meager biological material; as well as biological matrilineal descent. In order to analyze the genetic diversity of HVI and HVII regions of the mitochondrial genome in the population of Maracaibo; Zulia state; DNA samples from 50 persons were randomly selected; and hypervariable regions were amplified by polymerase chain reaction; subsequently sequenced by Sanger method and the fragments were separated by capillary electrophoresis; the differences were reported with respect to the Cambridge reference sequence. The genetic diversity of both regions was 0.9837 ± 0.0084; the nucleotide diversity 0.020001 ± 0.010201; Mean number of pairwise differences 12.160816 ± 5.588550; the power of discrimination 0.9640 and the random match probability of 0.0360. These results determined thathe haplogroups observed in the samples analyzed corresponded to Amerindian 68% (A 32% B 18%; C 4% and D 14%); Africans (L 20%); Europe 8 % (H 2%; K 2% U 2%; W 2%) and Asian (N 2%). The data generated from this study indicate that polymorphisms in the regions HVI and HVII are sufficiently informative to be used in forensic identification. Key words: Mitochondrial DNA; hypervariable regions; control region; forensic identification; Venezuelan population

Decay-accelerating factor 1 deficiency exacerbates leptospiral-induced murine chronic nephritis and renal fibrosis

Leptospirosis is a global zoonosis caused by pathogenic Leptospira, which can colonize the proximal renal tubules and persist for long periods in the kidneys of infected hosts. Here, we characterized the infection of C57BL/6J wild-type and Daf1-/- mice, which have an enhanced host response, with a virulent Leptospira interrogans strain at 14 days post-infection, its persistence in the kidney, and its link to kidney fibrosis at 90 days post-infection. We found that Leptospira interrogans can induce acute moderate nephritis in wild-type mice and is able to persist in some animals, inducing fibrosis in the absence of mortality. In contrast, Daf1-/- mice showed acute mortality, with a higher bacterial burden. At the chronic stage, Daf1-/- mice showed greater inflammation and fibrosis than at 14 days post-infection and higher levels at all times than the wild-type counterpart. Compared with uninfected mice, infected wild-type mice showed higher levels of IL-4, IL-10 and IL-13, with similar levels of α-smooth muscle actin, galectin-3, TGF-β1, IL-17, IFN-γ , and lower IL-12 levels at 90 days post-infection. In contrast, fibrosis in Daf1-/- mice was accompanied by high expression of α-smooth muscle actin, galectin-3, IL-10, IL-13, and IFN-γ, similar levels of TGF-β1, IL-12, and IL-17 and lower IL-4 levels. This study demonstrates the link between Leptospira-induced murine chronic nephritis with renal fibrosis and shows a protective role of Daf1.Instituto de Biotecnologia y Biologia MolecularFacultad de Ciencias Médica

Decay-accelerating factor 1 deficiency exacerbates leptospiral-induced murine chronic nephritis and renal fibrosis

Leptospirosis is a global zoonosis caused by pathogenic Leptospira, which can colonize the proximal renal tubules and persist for long periods in the kidneys of infected hosts. Here, we characterized the infection of C57BL/6J wild-type and Daf1-/- mice, which have an enhanced host response, with a virulent Leptospira interrogans strain at 14 days post-infection, its persistence in the kidney, and its link to kidney fibrosis at 90 days post-infection. We found that Leptospira interrogans can induce acute moderate nephritis in wild-type mice and is able to persist in some animals, inducing fibrosis in the absence of mortality. In contrast, Daf1-/- mice showed acute mortality, with a higher bacterial burden. At the chronic stage, Daf1-/- mice showed greater inflammation and fibrosis than at 14 days post-infection and higher levels at all times than the wild-type counterpart. Compared with uninfected mice, infected wild-type mice showed higher levels of IL-4, IL-10 and IL-13, with similar levels of α-smooth muscle actin, galectin-3, TGF-β1, IL-17, IFN-γ , and lower IL-12 levels at 90 days post-infection. In contrast, fibrosis in Daf1-/- mice was accompanied by high expression of α-smooth muscle actin, galectin-3, IL-10, IL-13, and IFN-γ, similar levels of TGF-β1, IL-12, and IL-17 and lower IL-4 levels. This study demonstrates the link between Leptospira-induced murine chronic nephritis with renal fibrosis and shows a protective role of Daf1.Instituto de Biotecnologia y Biologia MolecularFacultad de Ciencias Médica

Neutrophil extracellular traps are involved in the innate immune response to infection with <i>Leptospira</i>

NETosis is a process by which neutrophils extrude their DNA together with bactericidal proteins that trap and/or kill pathogens. In the present study, we evaluated the ability of Leptospira spp. to induce NETosis using human ex vivo and murine in vivo models. Microscopy and fluorometric studies showed that incubation of human neutrophils with Leptospira interrogans serovar Copenhageni strain Fiocruz L1-130 (LIC) resulted in the release of DNA extracellular traps (NETs). The bacteria number, pathogenicity and viability were relevant factors for induction of NETs, but bacteria motility was not. Entrapment of LIC in the NETs resulted in LIC death; however, pathogenic but not saprophytic Leptospira sp. exerted nuclease activity and degraded DNA. Mice infected with LIC showed circulating NETs after 2 days post-infection (dpi). Depletion of neutrophils with mAb1A8 significantly reduced the amount of intravascular NETs in LIC-infected mice, increasing bacteremia at 3 dpi. Although there was a low bacterial burden, scarce neutrophils and an absence of inflammation in the early stages of infection in the kidney and liver, at the beginning of the leptospiruric phase, the bacterial burden was significantly higher in kidneys of neutrophil-depleted-mice compared to non-depleted and infected mice. Surprisingly, interstitial nephritis was of similar intensity in both groups of infected mice. Taken together, these data suggest that LIC triggers NETs, and that the intravascular formation of these DNA traps appears to be critical not only to prevent early leptospiral dissemination but also to preclude further bacterial burden.Instituto de Biotecnologia y Biologia MolecularFacultad de Ciencias ExactasFacultad de Ciencias Médica