372 research outputs found
Clonación y análisis de la estructura primaria de quitina sintasas de entamoeba histolytica: perfil de expresión durante el enquistamiento
Entamoeba histolytica, parásito responsable de la amibiasis, presenta dos etapas en su ciclo de vida: trofozoÃto y quiste. Los quistes (forma infectiva) poseen una pared compuesta principalmente por quitina, polÃmero de ß-(1→4)-N-acetil-Dglucosamina cuya sÃntesis es catalizada por quitina sintasas (CHS). Las CHS se han descrito en hongos, insectos y nemátodos, pero no en protozoarios como Entamoeba. Se clonaron y secuenciaron dos genes CHS de E. histolytica. Se determinó que ambas EhCHS contienen segmentos transmembranales en sus extremos, y que la mayor similitud está restringida a la «región catalÃtica»; los aminoácidos importantes para la actividad de CHS están completamente conservados en ambas EhCH
Host tissue destruction by Entamoeba histolytica: molecules mediating adhesion, cytolysis, and proteolysis
Entamoeba histolytica, the protozoan parasite causing human amoebisis, has recently been found to comprise two genetically distinct forms, potentially pathogenic and constitutively nonpathogenic ones. Host tissue destruction by pathogenic forms is belived to result from cell functions mediaed by a lectin-type adherence receptor, a pore-forming peptide involved in host cell lysis, and abundant expression of cysteine proteinase(s). Isolation and molecular cloning of these amoeba products have provided the tools for structural analyses and manipulations of cell functions including comparisons between pathogenic and nonpathogenic forms
Absence of Erythrocyte Sequestration and Lack of Multicopy Gene Family Expression in Plasmodium falciparum from a Splenectomized Malaria Patient
BACKGROUND:To avoid spleen-dependent killing mechanisms parasite-infected erythrocytes (IE) of Plasmodium falciparum malaria patients have the capacity to bind to endothelial receptors. This binding also known as sequestration, is mediated by parasite proteins, which are targeted to the erythrocyte surface. Candidate proteins are those encoded by P. falciparum multicopy gene families, such as var, rif, stevor or PfMC-2TM. However, a direct in vivo proof of IE sequestration and expression of multicopy gene families is still lacking. Here, we report on the analysis of IE from a black African immigrant, who received the diagnosis of a malignant lymphoproliferative disorder and subsequently underwent splenectomy. Three weeks after surgery, the patient experienced clinical falciparum malaria with high parasitemia and circulating developmental parasite stages usually sequestered to the vascular endothelium such as late trophozoites, schizonts or immature gametocytes. METHODOLOGY/PRINCIPAL FINDINGS:Initially, when isolated from the patient, the infected erythrocytes were incapable to bind to various endothelial receptors in vitro. Moreover, the parasites failed to express the multicopy gene families var, A-type rif and stevor but expression of B-type rif and PfMC-2TM genes were detected. In the course of in vitro cultivation, the parasites started to express all investigated multicopy gene families and concomitantly developed the ability to adhere to endothelial receptors such as CD36 and ICAM-1, respectively. CONCLUSION/SIGNIFICANCE:This case strongly supports the hypothesis that parasite surface proteins such as PfEMP1, A-type RIFIN or STEVOR are involved in interactions of infected erythrocytes with endothelial receptors mediating sequestration of mature asexual and immature sexual stages of P. falciparum. In contrast, multicopy gene families coding for B-type RIFIN and PfMC-2TM proteins may not be involved in sequestration, as these genes were transcribed in infected but not sequestered erythrocytes
Differences in the transcriptome signatures of two genetically related Entamoeba histolytica cell lines derived from the same isolate with different pathogenic properties
<p>Abstract</p> <p>Background</p> <p>The availability of two genetically very similar cell lines (A and B) derived from the laboratory isolate <it>Entamoeba histolytica </it>HM-1:IMSS, which differ in their virulence properties, provides a powerful tool for identifying pathogenicity factors of the causative agent of human amoebiasis. Cell line A is incapable inducing liver abscesses in gerbils, whereas interaction with cell line B leads to considerable abscess formation. Phenotypic characterization of both cell lines revealed that trophozoites from the pathogenic cell line B have a larger cell size, an increased growth rate <it>in vitro</it>, an increased cysteine peptidase activity and higher resistance to nitric oxide stress. To find proteins that may serve as virulence factors, the proteomes of both cell lines were previously studied, resulting in the identification of a limited number of differentially synthesized proteins. This study aims to identify additional genes, serving as virulence factors, or virulence markers.</p> <p>Results</p> <p>To obtain a comprehensive picture of the differences between the cell lines, we compared their transcriptomes using an oligonucleotide-based microarray and confirmed findings with quantitative real-time PCR. Out of 6242 genes represented on the array, 87 are differentially transcribed (≥two-fold) in the two cell lines. Approximately 50% code for hypothetical proteins. Interestingly, only 19 genes show a five-fold or higher differential expression. These include three <it>rab7 GTPases</it>, which were found with a higher abundance in the non-pathogenic cell line A. The <it>aig1-like GTPases</it>are of special interest because the majority of them show higher levels of transcription in the pathogenic cell line B. Only two molecules were found to be differentially expressed between the two cell lines in both this study and our previous proteomic approach.</p> <p>Conclusions</p> <p>In this study we have identified a defined set of genes that are differentially transcribed between the non-pathogenic cell line A and the pathogenic cell line B of <it>E. histolytica</it>. The identification of transcription profiles unique for amoebic cell lines with pathogenic phenotypes may help to elucidate the transcriptional framework of <it>E. histolytica </it>pathogenicity and serve as a basis for identifying transcriptional markers and virulence factors.</p
Experimental transmission of Zika virus by mosquitoes from central Europe
Mosquitoes collected in Germany in 2016, including Culex pipiens pipiens biotype pipiens, Culex torrentium and Aedes albopictus, as well as Culex pipiens pipiens biotype molestus (in colony since 2011) were experimentally infected with Zika virus (ZIKV) at 18 degrees C or 27 degrees C. None of the Culex taxa showed vector competence for ZIKV. In contrast, Aedes albopictus were susceptible for ZIKV but only at 27 degrees C, with transmission rates similar to an Aedes aegypti laboratory colony tested in parallel.Peer reviewe
Comparative evaluation of two rapid field tests for malaria diagnosis: Partec Rapid Malaria Test® and Binax Now® Malaria Rapid Diagnostic Test
<p>Abstract</p> <p>Background</p> <p>About 90% of all malaria deaths in sub-Saharan Africa occur in children under five years. Fast and reliable diagnosis of malaria requires confirmation of the presence of malaria parasites in the blood of patients with fever or history suggestive of malaria; hence a prompt and accurate diagnosis of malaria is the key to effective disease management. Confirmation of malaria infection requires the availability of a rapid, sensitive, and specific testing at an affordable cost. We compared two recent methods (the novel Partec Rapid Malaria Test<sup>® </sup>(PT) and the Binax Now<sup>® </sup>Malaria Rapid Diagnostic Test (BN RDT) with the conventional Giemsa stain microscopy (GM) for the diagnosis of malaria among children in a clinical laboratory of a hospital in a rural endemic area of Ghana.</p> <p>Methods</p> <p>Blood samples were collected from 263 children admitted with fever or a history of fever to the pediatric clinic of the Agogo Presbyterian Hospital. The three different test methods PT, BN RDT and GM were performed independently by well trained and competent laboratory staff to assess the presence of malaria parasites. Results were analyzed and compared using GM as the reference standard.</p> <p>Results</p> <p>In 107 (40.7%) of 263 study participants, <it>Plasmodium sp</it>. was detected by GM. PT and BN RDT showed positive results in 111 (42.2%) and 114 (43.4%), respectively. Compared to GM reference standard, the sensitivities of the PT and BN RDT were 100% (95% CI: 96.6-100) and 97.2% (95% CI: 92.0-99.4), respectively, specificities were 97.4% (95% CI: 93.6-99.3) and 93.6% (95% CI: 88.5-96.9), respectively. There was a strong agreement (kappa) between the applied test methods (GM vs PT: 0.97; <it>p </it>< 0.001 and GM vs BN RDT: 0.90; <it>p </it>< 0.001). The average turnaround time per tests was 17 minutes.</p> <p>Conclusion</p> <p>In this study two rapid malaria tests, PT and BN RDT, demonstrated a good quality of their performance compared to conventional GM. Both methods require little training, have short turnaround times, are applicable as well as affordable and can therefore be considered as alternative diagnostic tools in malaria endemic areas. The species of <it>Plasmodium </it>cannot be identified.</p
Evaluation of a duplex real-time PCR in human serum for simultaneous detection and differentiation of Schistosoma mansoni and Schistosoma haematobium infections - cross-sectional study
Background: We evaluated a one-tube multiplex real-time PCR targeting DNA of Schistosoma haematobium
complex and S. mansoni complex in serum samples obtained at different German diagnostic centers.
Methods: Simplex real-time PCR protocols for the detection of the multi-copy DNA-repeats Dra1 of
S. haematobium complex and Sm1-7 of S. mansoni complex in serum were combined to a new one-tube multiplex
format. The new PCR was subjected to full validation including evaluation in a diagnostic real-life setting with
travelers and migrants. PCR results were compared with those of stool and urine microscopy, serology, and
circulating cathodic antigen (CCA) rapid diagnostic tests in urine. Sensitivity and specificity of the diagnostic
approaches were analyzed using latent class analysis (LCA).
Results: LCA assessment indicated sensitivity and specificity of 94.9% and 98.4%, respectively, for serum PCR if
serology was included in the calculation, and 100% and 95.6%, respectively, if serology was not included as a
parameter not necessarily associated with active infection. Agreement between the compared diagnostic procedures at genus level was fair (kappa 0.273) if serology was included and moderate (kappa 0.420) if serology
was not included.
Discussion: The PCR assay proved to be highly reliable for the diagnosis of schistosomiasis in travelers and
migrants
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