Daily VMA (vanillyl mandelic acid) variation in urine in relation of fatigue sensation, stress and life stile of nurses on night shift.

Daily VMA variation in urine in relation of fatigue sensation, stress and life stile of nurses on night shift. To evaluate exhaustion objectively in hospital nurses, daily variation of urinaly VMA (vanillyl mandelic acid) excretion was measured in hospital nurses working in daytime and nighttime and it's correlation with the data from Psychological Stress Response Scale (PSRS) test, the burn-out scale, quality and quantity of sleep and daily life stile recorded throughout 24 hours were studied. VMA level in urine showed circadian rhythmic movement, higher in daytime and lower in nighttime. During night shift, VMA in urine in nighttime showed slightly higher level than during day shift but in the subsequent daytime after night shift, VMA level was stillkeeping normal circadian rhythm, resuling flatness of daily fluctuation of VMA level. Dissatisfaction of sleep during daily fluctation of VMA level. Dissatisfaction of sleep during daytime was noticed. Correlation between VMA level in urine and sense of tiredness, stresses or burn-out scale were not carfied in this study because of shortage of samples but working conditions might affect biologicalresponse of exhaustion

EpCAM (CD326) regulates intestinal epithelial integrity and stem cells via Rho-associated kinase

Humans with biallelic inactivating mutations in Epithelial Cell Adhesion Molecule (EpCAM) develop congenital tufting enteropathy (CTE). To gain mechanistic insights regarding EpCAM function in this disorder, we prepared intestinal epithelial cell (IEC) organoids and spheroids. IEC organoids and spheroids were generated fro

The Subaru/XMM-Newton Deep Survey (SXDS) - VI. Properties of Active Galactic Nuclei Selected by Optical Variability

We present the properties of active galactic nuclei (AGN) selected by optical variability in the Subaru/XMM-Newton Deep Field (SXDF). Based on the locations of variable components and light curves, 211 optically variable AGN were reliably selected. We made three AGN samples; X-ray detected optically non-variable AGN (XA), X-ray detected optically variable AGN (XVA), and X-ray undetected optically variable AGN (VA). In the VA sample, we found a bimodal distribution of the ratio between the variable component flux and the host flux. One of these two components in the distribution, a class of AGN with a faint variable component $i'_{\rm{vari}}\sim25$ mag in bright host galaxies $i'\sim21$ mag, is not seen in the XVA sample. These AGN are expected to have low Eddington ratios if we naively consider a correlation between bulge luminosity and black hole mass. These galaxies have photometric redshifts $z_{\rm{photo}}\sim0.5$ and we infer that they are low-luminosity AGN with radiatively inefficient accretion flows (RIAFs). The properties of the XVA and VA objects and the differences from those of the XA objects can be explained within the unified scheme for AGN. Optical variability selection for AGN is an independent method and could provide a complementary AGN sample which even deep X-ray surveys have not found.Comment: 9 pages, 10 figures, accepted for publication in Ap

Langerhans cell antigen capture through tight junctions confers preemptive immunity in experimental staphylococcal scalded skin syndrome

Epidermal LCs but not dermal DCs take up skin surface protein through intact tight junctions and mediate IgG1 antibody responses to bacterial antigen, conferring protective immunization

The Subaru/XMM-Newton Deep Survey (SXDS) - V. Optically Faint Variable Object Survey

We present our survey for optically faint variable objects using multi-epoch (8-10 epochs over 2-4 years) $i'$-band imaging data obtained with Subaru Suprime-Cam over 0.918 deg$^2$ in the Subaru/XMM-Newton Deep Field (SXDF). We found 1040 optically variable objects by image subtraction for all the combinations of images at different epochs. This is the first statistical sample of variable objects at depths achieved with 8-10m class telescopes or HST. The detection limit for variable components is $i'_{\rm{vari}}\sim25.5$ mag. These variable objects were classified into variable stars, supernovae (SNe), and active galactic nuclei (AGN), based on the optical morphologies, magnitudes, colors, and optical-mid-infrared colors of the host objects, spatial offsets of variable components from the host objects, and light curves. Detection completeness was examined by simulating light curves for periodic and irregular variability. We detected optical variability for $36\pm2$ ($51\pm3$ for a bright sample with $i'<24.4$ mag) of X-ray sources in the field. Number densities of variable obejcts as functions of time intervals $\Delta{t}$ and variable component magnitudes $i'_{\rm{vari}}$ are obtained. Number densities of variable stars, SNe, and AGN are 120, 489, and 579 objects deg$^{-2}$, respectively. Bimodal distributions of variable stars in the color-magnitude diagrams indicate that the variable star sample consists of bright ($V\sim22$ mag) blue variable stars of the halo population and faint ($V\sim23.5$ mag) red variable stars of the disk population. There are a few candidates of RR Lyrae providing a possible number density of $\sim10^{-2}$ kpc$^{-3}$ at a distance of $>150$ kpc from the Galactic center.Comment: 18 pages, 17 figures, accepted for publication in Ap

Association between intensive care unit delirium and delusional memory after critical care in mechanically ventilated patients

AimTo determine the relationship between the delirium of patients with mechanical ventilation during intensive care unit (ICU) stay and delusional memory after ICU discharge.DesignProspective cohort study.MethodsDelirium in adult patients who received mechanical ventilation for more than 24 hr was assessed twice daily using the Confusion Assessment Method for the ICU. Delusional memories were evaluated using the ICU Memory Tool 5–10 days after ICU discharge. The associations between the presence of delirium during the ICU stay and delusional memories were evaluated.ResultsOf 60 enrolled patients, 62% had delirium during their ICU stay, and 68% experienced delusional memories 5–10 days after discharge. Delirium during ICU stay was an independent factor to experience delusional memories following discharge. Preventing delirium during ICU stay might reduce delusional memory. We recommend that patients with delirium during their ICU stay should be carefully followed up after discharge from the ICU

RNA-Binding Protein Musashi1 Modulates Glioma Cell Growth through the Post-Transcriptional Regulation of Notch and PI3 Kinase/Akt Signaling Pathways

Musashi1 (MSI1) is an RNA-binding protein that plays critical roles in nervous-system development and stem-cell self-renewal. Here, we examined its role in the progression of glioma. Short hairpin RNA (shRNA)-based MSI1-knock down (KD) in glioblastoma and medulloblastoma cells resulted in a significantly lower number of self renewing colony on day 30 (a 65% reduction), compared with non-silencing shRNA-treated control cells, indicative of an inhibitory effect of MSI1-KD on tumor cell growth and survival. Immunocytochemical staining of the MSI1-KD glioblastoma cells indicated that they ectopically expressed metaphase markers. In addition, a 2.2-fold increase in the number of MSI1-KD cells in the G2/M phase was observed. Thus, MSI1-KD caused the prolongation of mitosis and reduced the cell survival, although the expression of activated Caspase-3 was unaltered. We further showed that MSI1-KD glioblastoma cells xenografted into the brains of NOD/SCID mice formed tumors that were 96.6% smaller, as measured by a bioluminescence imaging system (BLI), than non-KD cells, and the host survival was longer (49.3±6.1 days vs. 33.6±3.6 days; P<0.01). These findings and other cell biological analyses suggested that the reduction of MSI1 in glioma cells prolonged the cell cycle by inducing the accumulation of Cyclin B1. Furthermore, MSI1-KD reduced the activities of the Notch and PI3 kinase-Akt signaling pathways, through the up-regulation of Numb and PTEN, respectively. Exposure of glioma cells to chemical inhibitors of these pathways reduced the number of spheres and living cells, as did MSI1-KD. These results suggest that MSI1 increases the growth and/or survival of certain types of glioma cells by promoting the activation of both Notch and PI3 kinase/Akt signaling

Physical restraints in intensive care units: a national questionnaire survey of physical restraint use for critically ill patients undergoing invasive mechanical ventilation in Japan

AimsPhysical restraints are often used for patients undergoing mechanical ventilation to protect important medical equipment. However, they have adverse physical and psychological effects and could pose ethical hazards. Physical restraint use varies by country, but so far there is little understanding of the frequency of physical restraint use among intensive care unit (ICU) patients in Japan. The present study aims to describe the frequency of physical restraint use among Japanese patients undergoing mechanical ventilation. Additionally, it attempts to verify the hypothesis that insufficient human resources have increased the frequency of physical restraints.MethodsWe undertook a cross-sectional online open anonymous survey of ICU nurses using a self-administered questionnaire to examine the use of physical restraints for patients undergoing invasive mechanical ventilation in Japan.ResultsWe obtained 175 responses, of which 46 were excluded. Of the respondents, 43% reported that physical restraints were used for more than 75% of mechanically ventilated patients. Intensive care units with a higher frequency of physical restraint use had a significantly greater number of beds per nurse compared to those with a lower frequency; however, after adjusting for the number of beds in the ICU and hospital type in a logistic regression analysis, the number of beds per nurse was no longer significantly related to the use of physical restraints.ConclusionsPhysical restraints are commonly used among patients undergoing mechanical ventilation in Japan. A systematic approach to reducing physical restraint use among mechanically ventilated patients is needed.Brief Communicatio