Global exponential stabilization of language constrained switched linear discrete-time system based on the s-procedure approach

This paper considers global exponential stabilization (GES) of switched linear discrete-time system under language constraint which is generated by non-deterministic finite state automata. A technique in linear matrix inequalities called S-procedure is employed to provide sufficient conditions of GES which are less conservative than the existing Lyapunov-Metzler condition. Moreover, by revising the construction of Lyapunov matrices and the corresponding switching control policy, a more flexible result is obtained such that stabilization path at each moment might be multiple. Finally, a numerical example is given to illustrate the effectiveness of the proposed results

Clinical application, evaluation and analysis of influencing factors analysis of on tuberculosis ?-Interferon enzyme-linked immunosorbent assay

Objective: To assess sensitivity and specificity of the interferon gamma release assay test, and to pinpoint the influencing factors that should be taken care of in clinical application. Methods: The study was conducted at the First People's Hospital in the Yunnan Province of China from October 2018 to March 2019, and comprised samples collected from outpatient and inpatients. To detect mycobacterium tuberculosis, acid-fast staining on sputum smear was performed on relevant tissues suspected of extrapulmonary tuberculosis. Tuberculosis interferon gamma release assay test and pathology samples were examined. Tuberculosis-specific cell reaction assay kit was used for sampling. SysmexXN-2000 haematology analyser, VACUETTE SRS100/II and Beckman Coulter AU5800 were used to perform various analyses. Data was grouped and analysed using R statistical software. Results: Of the 960 samples, 516(53.75%) cases tested positive for tuberculosis infection and 444(46.25%) tested negative. The sensitivity of the pathological results was 86% and the specificity was 96%. The sensitivity of the interferon gamma release assay test was 95% and specificity 82%. Interferon release test, pathological results and final diagnosis showed significant comparisons (p<0.05). Significant relationships were also established for factors, such as age, interferon release quantity, lymphocyte, C-reactive protein and counts of mono-nuclear cell (p<0.05). Conclusions: Interferon gamma release assay test was found to have high consistency with pathological results and final diagnosis and can be used as a subsidiary to traditional clinical imaging and pathological judgment. Key Words: Tuberculosis, Interferon release, CRP, Hormone use. Continuous...

A new transient expression system for large-scale production of recombinant proteins in plants based on air-brushing an Agrobacterium suspension

Plant transient expression using virus-based vectors is advantageous when high level of gene expression is desired within a short time. In this study, a new system, named “air-brush,” has been developed to facilitate a scale-up production of recombinant proteins in plants. GFP was expressed successfully in Nicotiana benthamiana (Nb) plants by air-brushing an Agrobacterium suspension that contained the TMV-based vector p35S-30B-GFP. Key factors influencing the gene expression were optimized, including the Agrobacterium cell density, seedling age, and the growth temperature of plant materials. In addition, the pharmaceutical protein human acidic fibroblast growth factor (ha FGF) was also expressed in Nb plants by the air-brush system. The results demonstrated that using this system is highly advantageous; it is convenient, quick, easily scaled-up, and has a higher expression efficiency than leaf infiltration