Population genetic structure of 2 species from Caspian Sea (Rutilus frissi kutum and Rutilus rutilus) and 2 species from Persian Gulf and Oman Sea (Rachycentron canadum and Eleutheronema tetradactylum) using Molecular Marker

Population structure of 2 species in the Caspian Sea (Rutilus frissi kutum and Rutilus rutilus) and 2 species in the Persian Gulf and Oman sea (Rachycentron canadum and Polynemus Plebeius) were studid by using Microsatellite and RAPD. For this 210 specimens of kutum (Khoshkrud, Tonekabon, Gorganrud, Anzali Lagoon and Kura River mouth), 90 (Gorgan Bay, Anzali Lagoon and Volga), 184 of cobia (Bushehr, daier, Bandarabass, bazm, lengeh) and 235 of (Khozestan, Bushehr, Bandarabass and chabahar) were sampled. Genomic DNA was extracted of fin using the phenol-chloroform .The quantity and quality of DNA from samples were assessed by spectrophptometer and 1% agarose gel electro-phoresis. PCR was carried out using microsatellite and RAPD primers. PCR products were separated on 8% polyacrylamide gels that were stained using silver nitrate. Result showed that in kutum the average of expected and observed heterozygosity was 0.54 and 0.49 respectively. Significant deviations from Hardy-Weinberg expectations were observed almost in all of location (P≤0.01, (P≤0.05). Using Fst and Rst there was significant difference between locations (P≤0.01). In study of Rutilus rutiluscaspicu the average of expected and observed heterozygosity was 0.5 and 0.7 respectively. Fst didn t show significant difference between iranian locations (P≥ 0.01) but it was Significant between Iranian populations and Russian populations (P≤ 0.05). In cobia the average of expected and observed heterozygosity was 0.655 and 0.874 respectively. Significant deviations from Hardy-Weinberg expectations were observed almost in all of location (P ≤0.01, (P≤ 0.05)). Significant differences (P<0.01) weren't observed between Rst recorded in the same region specimens studied but were observed between Rst recorded in the different region specimens studied. In Polynemus Plebeius, the observed heterozygosity in the Bushehr and Bandar abbas (0.22±0.13) was the highest and in Chabahar the lowest (0.171±0.175).Key word: Population Genetic, Rutilus frissi kutum ,Rutilus rutilus, Caspian Sea, Rachycentron canadum , Polynemus Plebeius, Persian Gulf and Oman sea

Health risk assessment of heavy metal intake due to fish consumption in the Sistan region, Iran

The heavy metal (Pb, Cd, Cr, and Ni) content of a fish species consumed by the Sistan population and its associated health risk factors were investigated. The mean concentrations of Pb, Cd, and Cr were slightly higher than the standard levels. The Ni content of fish was below the maximum guideline proposed by the US Food and Drug Administration (USFDA). The average estimated weekly intake was significantly below the provisional tolerable intake based on the FAO and WHO standards for all studied metals. The target hazard quotients (THQ) of all metals were below 1, showing an absence of health hazard for the population of Sistan. The combined target hazard quotient for the considered metals was 26.94 × 10â��3. The cancer risk factor for Pb (1.57 × 10â��7) was below the acceptable lifetime carcinogenic risk (10â��5). The results of this study reveal an almost safe level of Pb, Cd, Cr, and Ni contents in the fish consumed by the Sistan population

Patency rate and factors affecting the clinical outcome of patients with aortoiliac artery occlusion undergoing intra-arterial stenting

Background Occluding plaques are commonly found inthe infrarenal aorta and the iliac artery in patients with peripheralarterial disease (PAD). Aortoiliacocclusive disease(AIOD) occurs commonly in patients with PAD.Methods: This is a prospective study was carried out between2015 and 2017 in Sina hospital. Patients with occlusionin aortoiliac artery and its branches were included.Stent was placed for patients, if they had any obstructionor stenosis in aortoiliac artery in angiography. Patientswere examined in terms of clinical status and ABI calculationand underwent Color Doppler ultrasound of aortoiliacartery to determine patency rate at 1, 3, 12, and 24months after stent placement.Results: The mean age of patients was 64.60 (&plusmn;10.41).The main symptoms of the patients were claudication(41.8%), ulcer (27.3%), pain at rest (20%), and gangrene(20%). The lesion was bilateral in 21 patients (38.2%).According to TASC stratification, lesions were of type A in26 patients (47.3%), type B in 4 patients (7.3%), type Cin 5 patients (9.1%), and type D in 20 patients (36.4%).The six-month, one-year, and two-year patency rates were89.1%, 83.6%, and 72.7%, respectively. The mean annualABI of patients was 0.79 (&plusmn;0.11) and two year ABIwas 0.68 (&plusmn;0.1).Conclusion: It seems that stenting and angioplasty areeffective in improving patients&rsquo; conditions and result in agood short and medium-term patency

Molecular genetics study on population of Salmo trutta caspius spring and autumn forms in Southern Caspian Sea using microsatellite marker method basin and establish an alive gene bank

Caspian brown trout (Salmo trutta caspius) is an important from the point of economy. In this study genetic diversity of (Salmo trutta caspius) was investigated using microsatellite markers from two regions of the Iranian coastline of Southern Caspian Sea (Cheshmeh Kileh River in Mazandaran Province and Karganrud River in Gilan Province). The purpose of this research was the study of Salmo trutta caspius possible populations related to genetic diversity and population structure in the Caspian Sea and introducing the useful genetic markers. For this purpose the number 225 fishes from two Rivers were collected. DNA was extracted and amplified with 16 pairs of microsatellite primers using Polymerase Chain Reaction (PCR). The amplified products were loaded on polyacrylamide gels and bands were analyzed using Gene Alex and Pop Gene soft wares. Observed and expected heterozygosity averages were 0.46 ± 0.09 and 0.60 ± 0.10, respectively. According to the results from this study, Genetic polymorphism has been seen in this population

Health risk assessment of heavy metal intake due to fish consumption in the Sistan region, Iran

The heavy metal (Pb, Cd, Cr, and Ni) content of a fish species consumed by the Sistan population and its associated health risk factors were investigated. The mean concentrations of Pb, Cd, and Cr were slightly higher than the standard levels. The Ni content of fish was below the maximum guideline proposed by the US Food and Drug Administration (USFDA). The average estimated weekly intake was significantly below the provisional tolerable intake based on the FAO and WHO standards for all studied metals. The target hazard quotients (THQ) of all metals were below 1, showing an absence of health hazard for the population of Sistan. The combined target hazard quotient for the considered metals was 26.94 × 10â��3. The cancer risk factor for Pb (1.57 × 10â��7) was below the acceptable lifetime carcinogenic risk (10â��5). The results of this study reveal an almost safe level of Pb, Cd, Cr, and Ni contents in the fish consumed by the Sistan population

Venoplasty and Venous Stenting in Patients with Chronic Venous Insufficiency in the Lower Extremities

Background: Venoplasty and stenting is a minimally invasive therapy that can be used for patients with deep venous insufficiency in the lower extremities. This study aimed at investigating the effect of venoplasty and venous stenting in patients with chronic venous insufficiency in the lower limbs. Methods: This prospective case-series study recruited patients with chronic deep venous insufficiency in the lower limbs candidated for venoplasty in the Vascular Clinic of Sina Hospital in Tehran, Iran. Venoplasty and stenting was done if the deep venous system in the lower extremities had stenosis or obstruction on venography. The patients were visited 1, 3, and 6 months after venoplasty to assess their symptoms, venous clinical severity, and venous disability. Primary and secondary patency was evaluated with Doppler ultrasound. Results: Seventy-three patients were included in the study. The follow-up of the patients’ clinical symptoms showed significant improvement rates of about 90%, 88.7%, 92.5%, and 100% in claudication, edema, pain, and ulcers-respectively- only 1 month after the procedure. The stent patency rates were 93.2, 91.5, and 92.4 in the 1st, 2nd, 3rd, and 6th postprocedural months, correspondingly. The venous clinical severity score and the venous disability score before the procedure were 14.2 and 2.73, respectively, which were decreased to 5 and 1.1, correspondingly, at 6 months’ follow-up (p value < 0.001). Conclusion: Venoplasty and stenting in our patients with chronic deep venous insufficiency in the lower extremities conferred a significant improvement in clinical symptoms and a high percentage of patency

Comparison of results of placement of cuffed –tunneled hemodialysis catheter in internal jugular vein with subclavian vein for long -term dialysis

Aim of the study was to comparison between internal jugular vs. subclavian vein cuffed tunnel catheter placement for dialysis. Material and methods. Cases who required central venous catheter for dialysis were included in this study. Forty cases were included in this study and divided to two groups. Catheters were placed randomly in internal jugular vein or subclavian. Patients were followed for 6 months. Early and late complications of catheter’s placement were recorded. Analysis was done using Spss ver 13.0 (Chicago, IL, USA). Results. There were no significant differences between subclavian and internal jugular vein regarding occurrence of infection resulted in extraction or treatment. Also there were no significant differences regarding occurrence of thrombosis resulted in extraction or treatment. Failure rate was significantly higher in cases with internal jugular vein catheter compared to cases with subclavian vein catheter (p=0.04). Conclusion. Failure rate was significantly higher in cases with internal jugular vein catheter compared to subclavian cathether. Subclavian catheter is more appropriate route for catheter placement

Molecular interaction of fibrinogen with zeolite nanoparticles

Fibrinogen is one of the key proteins that participate in the protein corona composition of many types of nanoparticles (NPs), and its conformational changes are crucial for activation of immune systems. Recently, we demonstrated that the fibrinogen highly contributed in the protein corona composition at the surface of zeolite nanoparticles. Therefore, understanding the interaction of fibrinogen with zeolite nanoparticles in more details could shed light of their safe applications in medicine. Thus, we probed the molecular interactions between fibrinogen and zeolite nanoparticles using both experimental and simulation approaches. The results indicated that fibrinogen has a strong and thermodynamically favorable interaction with zeolite nanoparticles in a non-cooperative manner. Additionally, fibrinogen experienced a substantial conformational change in the presence of zeolite nanoparticles through a concentration-dependent manner. Simulation results showed that both E- and D-domain of fibrinogen are bound to the EMT zeolite NPs via strong electrostatic interactions, and undergo structural changes leading to exposing normally buried sequences. D-domain has more contribution in this interaction and the C-terminus of γ chain (γ 377–394 ), located in D-domain, showed the highest level of exposure compared to other sequences/residues