Cats with diabetes mellitus have diastolic dysfunction in the absence of structural heart disease

Diabetes mellitus (DM) can result in cardiovascular dysfunction and heart failure characterized by diastolic dysfunction with or without the presence of systolic dysfunction in people and laboratory animals. The objective of this prospective study was to determine if cats with newly diagnosed DM had myocardial dysfunction and, if present, whether it would progress if appropriate antidiabetic therapy was commenced. Thirty-two diabetic cats were enrolled and received baseline echocardiographic examination; of these, 15 cats were re-examined after 6 months. Ten healthy age- and weight-matched cats served as controls. Diabetic cats at diagnosis showed decreased diastolic, but not systolic function, when compared to healthy controls, with lower mitral inflow E wave (E) and E/E' than controls. After 6 months, E and E/IVRT' decreased further in diabetic cats compared to the baseline evaluation. After excluding cats whose DM was in remission at 6 months, insulin-dependent diabetic cats had lower E, E/A and E' than controls. When classifying diastolic function according to E/A and E'/A', there was shift towards impaired relaxation patterns at 6 months. All insulin-dependent diabetic cats at 6 months had abnormal diastolic function. These results indicate that DM has similar effects on diastolic function in feline and human diabetics. The dysfunction seemed to progress rather than to normalize after 6 months, despite antidiabetic therapy. In cats with pre-existing heart disease, the development of DM could represent an important additional health risk

Genetic Basis of Nonsyndromic Sensorineural Hearing Loss in the Sub-Saharan African Island Population of São Tomé and Príncipe: The Role of the DFNB1 Locus?

Hearing loss (HL) is a common condition with both genetic and environmental causes, and it greatly impacts global health. The prevalence of HL is reportedly higher in developing countries such as the Sub-Saharan African island of São Tomé and Príncipe, where the deaf community is estimated to be less than 1% of the population. We investigated the role of the DFNB1 locus (GJB2 and GJB6 genes) in the etiology of nonsyndromic sensorineural hearing loss (NSSHL) in São Tomé and Príncipe. A sample of 316 individuals, comprising 136 NSSHL patients (92 bilateral, 44 unilateral) and 180 controls, underwent a clinical and audiological examination. Sequencing of the GJB2 coding region and testing for the (GJB6-D13S1830) and del(GJB6-D13S1854) GJB6 deletions were performed. A total of 311 out of 316 individuals were successfully analyzed regarding the GJB2 and GJB6 genetic variations, respectively. The frequency of the GJB2 coding mutations in patients and controls was low. Some of those coding mutations are the most commonly found in Eurasian and Mediterranean populations and have also been identified in Portugal. None of the GJB6 deletions was present. The presence of certain coding variants in São Tomé and Príncipe suggests a non-Sub-Saharan genetic influx and supports the previously reported genetic influx from European (mainly Portuguese) ancestors. In summary, DFNB1 locus does not appear to be a major contributor to NSSHL in São Tomé and Príncipe. However, the presence of both pathogenic and likely pathogenic mutations in GJB2 suggests that GJB2-related NSSHL might still occur in this population, warranting further research on GJB2 testing in NSSHL cases

Smc5/6 coordinates formation and resolution of joint molecules with chromosome morphology to ensure meiotic divisions

During meiosis, Structural Maintenance of Chromosome (SMC) complexes underpin two fundamental features of meiosis: homologous recombination and chromosome segregation. While meiotic functions of the cohesin and condensin complexes have been delineated, the role of the third SMC complex, Smc5/6, remains enigmatic. Here we identify specific, essential meiotic functions for the Smc5/6 complex in homologous recombination and the regulation of cohesin. We show that Smc5/6 is enriched at centromeres and cohesin-association sites where it regulates sister-chromatid cohesion and the timely removal of cohesin from chromosomal arms, respectively. Smc5/6 also localizes to recombination hotspots, where it promotes normal formation and resolution of a subset of joint-molecule intermediates. In this regard, Smc5/6 functions independently of the major crossover pathway defined by the MutLγ complex. Furthermore, we show that Smc5/6 is required for stable chromosomal localization of the XPF-family endonuclease, Mus81-Mms4Eme1. Our data suggest that the Smc5/6 complex is required for specific recombination and chromosomal processes throughout meiosis and that in its absence, attempts at cell division with unresolved joint molecules and residual cohesin lead to severe recombination-induced meiotic catastroph

Conceptual Framework for Managing Uncertainty in a Collaborative Agri-Food Supply Chain Context

[EN] Agri-food supply chains are subjected to many sources of uncertainty. If these uncertainties are not managed properly, they can have a negative impact on the agri-food supply chain (AFSC) performance, its customers, and the environment. In this sense, collaboration is proposed as a possible solution to reduce it. For that, a conceptual framework (CF) for managing uncertainty in a collaborative context is proposed. In this context, this paper seeks to answer the following research questions: What are the existing uncertainty sources in the AFSCs? Can collaboration be used to reduce the uncertainty of AFSCs? Which elements can integrate a CF for managing uncertainty in a collaborative AFSC? The CF proposal is applied to the weather source of uncertainty in order to show its applicability.The first author acknowledges the partial support of the Program of Formation of University Professors of the Spanish Ministry of Education, Culture, and Sport (FPU15/03595). The other authors acknowledge the partial support of the Project 691249, RUC-APS: Enhancing and implementing Knowledge based ICT solutions within high Risk and Uncertain Conditions for Agriculture Production Systems, funded by the EU under its funding scheme H2020-MSCA-RISE-2015.Esteso-Álvarez, A.; Alemany Díaz, MDM.; Ortiz Bas, Á. (2017). Conceptual Framework for Managing Uncertainty in a Collaborative Agri-Food Supply Chain Context. IFIP Advances in Information and Communication Technology. 506:715-724. https://doi.org/10.1007/978-3-319-65151-4_64S715724506Taylor, D.H., Fearne, A.: Towards a framework for improvement in the management of demand in agri-food supply chains. Supply Chain Manag. Int. J. 11, 379–384 (2006)Matopoulos, A., Vlachopoulou, M., Manthou, V., Manos, B.: A conceptual framework for supply chain collaboration: empirical evidence from the agri-food industry. Supply Chain Manag. Int. J. 12, 177–186 (2007)Ahumada, O., Villalobos, J.R.: Application of planning models in the agri-food supply chain: a review. Eur. J. Oper. Res. 196, 1–20 (2009)Tsolakis, N.K., Keramydas, C.A., Toka, A.K., Aidonis, D.A., Iakovou, E.T.: Agrifood supply chain management: a comprehensive hierarchical decision-making framework and a critical taxonomy. Biosyst. Eng. 120, 47–64 (2014)van der Vorst, J.G., Da Silva, C.A., Trienekens, J.H.: Agro-industrial supply chain management: Concepts and applications. FAO (2007)Borodin, V., Bourtembourg, J., Hnaien, F., Kabadie, N.: Handling uncertainty in agricultural supply chain management: a state of the art. Eur. J. Oper. Res. 254, 348–359 (2016)van der Vorst, J.G.A.J., Beulens, A.J.M.: Identifying sources of uncertainty to generate supply chain redesign strategies. Int. J. Phys. Distrib. Logist. Manag. 32, 409–430 (2000)Klosa, E.: A concept of models for supply chain speculative risk analysis and management. J. Econ. Manag. 12, 45–59 (2013)Samson, S., Reneke, J.A., Wiecek, M.M.: A review of different perspectices on uncertainty and risk and an alternative modeling paradigm. Reliab. Eng. Syst. Saf. 94, 558–567 (2009)Backus, G.B.C., Eidman, V.R., Dijkhuizen, A.A.: Farm decision making under risk and uncertainty. Neth. J. Agric. Sci. 45, 307–328 (1997)van der Vorst, J.G.: Effective food supply chains; Generating, modelling and evaluating supply chain scenarios. (2000)Amorim, P., Günther, H.O., Almada-Lobo, B.: Multi-objective integrated production and distribution planning of perishable products. Int. J. Prod. Econ. 138, 89–101 (2012)Amorim, P., Meyr, H., Almeder, C., Almada-Lobo, B.: Managing perishability in production-distribution planning: a discussion and review. Flex. Serv. Manuf. 25, 389–413 (2013)Costa, C., Antonucci, F., Pallottino, F., Aguzzi, J., Sarria, D., Menesatti, P.: A review on agri-food supply chain traceability by means of RFID technology. Food Bioprocess Technol. 6, 353–366 (2013)Pahl, J., Voss, S.: Integrating deterioration and lifetime constraints in production and supply chain planning: a survey. Eur. J. Oper. Res. 238, 654–674 (2014)Grillo, H., Alemany, M.M.E., Ortiz, A.: A review of Mathematical models for supporting the order promising process under Lack of Homogeneity in product and other sources of uncertainty. Comput. Ind. Eng. 91, 239–261 (2016)Zwietering, M.H., van’t Riet, K.: Modelling of the quality of food: optimization of a cooling chain. In: Management Studies and the Agri-business: Management of Agri-chains, Wageningen, The Netherlands, pp. 108–117 (1994)Akkerman, R., Farahani, P., Grunow, M.: Quality, safety and sustainability in food distribution: a review of quantitative operations management approaches and challenges. Spectrum 32, 863–904 (2010)Apaiah, R.K., Hendrix, E.M.T., Meerdink, G., Linnemann, A.R.: Qualitative methodology for efficient food chain design. Trends Food Sci. Technol. 16, 204–214 (2005)Lehmann, R.J., Reiche, R., Schiefer, G.: Future internet and the agri-food sector: State-of-the-art in literature and research. Comput. Electron. Agric. 89, 158–174 (2012)Kusumastuti, R.D., van Donk, D.P., Teunter, R.: Crop-related harvesting and processing planning: a review. Int. J. Prod. Econ. 174, 76–92 (2016)Dreyer, H.C., Strandhagen, J.O., Hvolby, H.H., Romsdal, A., Alfnes, E.: Supply chain strategies for speciality foods: a Norwegian case study. Prod. Plan. Control 27, 878–893 (2016)Baghalian, A., Rezapour, S., Farahani, R.Z.: Robust supply chain network design with service level against disruptions and demand uncertainties: a real-life case. Eur. J. Oper. Res. 227, 199–215 (2013)Aggarwal, S., Srivastava, M.K.: Towards a grounded view of collaboration in Indian agri-food supply chains: a qualitative investigation. Br. Food J. 115, 1085–1106 (2016)Teimoury, E., Nedaei, H., Ansari, S., Sabbaghi, M.: A multi-objective analysis for import quota policy making in a perishable fruit and vegetable supply chain: a system dynamics approach. Comput. Electron. Agric. 93, 37–45 (2013)Opara, L.U.: Traceability in agriculture and food supply chain: a review of basic concepts, technological implications, and future prospects. J. Food Agric. Environ. 1, 101–106 (2003)Kruize, J.W., Wolfert, S., Goense, D., Scholten, H., Beulens, A., Veenstra, T.: Integrating ICT applications for farm business collaboration processes using Fl Space. In: 2014 Annual SRII Global Conference, pp. 232–240. IEEE (2014)Oriade, C.A., Dillon, C.R.: Developments in biophysical and bioeconomic simulation of agricultural systems: a review. Agric. Econ. 17, 45–58 (1997)Camarinha-Matos, L.M., Afsarmanesh, H.: Collaborative networks: value creation in a knowledge society. In: Wang, Kesheng, Kovacs, G.L., Wozny, Michael, Fang, Minglun (eds.) PROLAMAT 2006. IIFIP, vol. 207, pp. 26–40. Springer, Boston, MA (2006). doi: 10.1007/0-387-34403-9_4Prima Dania, W.A., Xing, K., Amer, Y.: Collaboration and sustainable agri-food supply chain: a literature review. MATEC Web Conf. 58 (2016)Simatupang, T.M., Sridharan, R.: The collaborative index: a measure for supply chain collaboration. Int. J. Phys. Distrib. Logist. Manag. 35, 44–62 (2005)Fischer, C., Hartmann, M., Reynolds, N., Leat, P., Revoredo-Giha, C., Henchion, M., Albisu, L.M., Gracia, A.: Factors influencing contractual choice and sustainable relationships in European agri-food supply chains. Eur. Rev. Agric. Econ. 36, 541–569 (2009

A complex scenario of tuberculosis transmission is revealed through genetic and epidemiological surveys in Porto

Tuberculosis (TB) incidence is decreasing worldwide and eradication is becoming plausible. In low-incidence countries, intervention on migrant populations is considered one of the most important strategies for elimination. However, such measures are inappropriate in European areas where TB is largely endemic, such as Porto in Portugal. We aim to understand transmission chains in Porto through a genetic characterization of Mycobacterium tuberculosis strains and through a detailed epidemiological evaluation of cases.This work was developed under the scope of the project NORTE-01-0145-FEDER-000013, supported by the Northern Portugal Regional Operational Programme (NORTE 2020), under the Portugal 2020 Partnership Agreement, through the European Regional Development Fund (FEDER), and supported by contributions from Iceland, Liechtenstein and Norway through the European Economic Area Grants under the Public Health Initiative programme, (PT06, Project 000138DT1). TR is supported by the Portuguese Foundation for Science and Technology (FCT) through a post-doctoral grant (SFRH/BPD/108126/2015)info:eu-repo/semantics/publishedVersio

Total antioxidant activity and trace elements in human milk: the first 4 months of breast-feeding

The content of many nutrients in breast milk are dependent on the nutritional status of the lactating woman. This is particularly true for fat and water-soluble vitamins, some of which have antioxidant properties. The aim of the study entertained herein was to evaluate the changes in total antioxidant status of human milk during the first 4 months of lactation, and to correlate such changes with the contents in specific antioxidant oligoelements (Cu, Zn, Mn and Se). Milk samples were collected from (31) lactating women recruited at the Service of Obstetrics of the Hospital de São João in Porto, after 1, 4, 8, 12 and 16 weeks after birth. The total antioxidant status (TAS) of human milk was measured by the Randox® commercial kit and trace metals by ICP-MS (inductively coupled plasma-mass spectrometry). The results found for TAS and oligoelements under study show a decrease in the concentration of these parameters from 7 days to 4 months of breast-feeding and significant correlations (p < 0.05) were found between TAS and Cu, Zn and Se (not Mn). The decreases of Cu, Zn and Se were also correlated, but not proportional between them, suggesting diverse excretion mechanisms for all. Between primipara and multipara women, a significant difference was found only for Cu and Zn concentrations at 7 days of lactation, but not for the other metals or TAS. With respect to the mother’s age, no correlation was found, either for trace metal concentrations or TAS

IFNG +874T/A polymorphism is not associated with American tegumentary leishmaniasis susceptibility but can influence Leishmania induced IFN-γ production

<p>Abstract</p> <p>Background</p> <p>Interferon-gamma is a key cytokine in the protective responses against intracellular pathogens. A single nucleotide polymorphism (SNP) located in the first intron of the human IFN-γ gene can putatively influence the secretion of cytokine with an impact on infection outcome as demonstrated for tuberculosis and other complex diseases. Our aim was to investigate the putative association of IFNG+874T/A SNP with American tegumentary leishmaniasis (ATL) and also the influence of this SNP in the secretion of IFN-γ <it>in vitro</it>.</p> <p>Methods</p> <p>Brazilian ATL patients (78 cutaneous, CL, and 58 mucosal leishmaniasis, ML) and 609 healthy volunteers were evaluated. The genotype of +874 region in the IFN-γ gene was carried out by Amplification Refractory Mutational System (ARMS-PCR). <it>Leishmania</it>-induced IFN-γ production on peripheral blood mononuclear cell (PBMC) culture supernatants was assessed by ELISA.</p> <p>Results</p> <p>There are no differences between +874T/A SNP frequency in cases and controls or in ML versus CL patients. Cutaneous leishmaniasis cases exhibiting AA genotype produced lower levels of IFN-γ than TA/TT genotypes. In mucosal cases, high and low IFN-γ producers were clearly demonstrated but no differences in the cytokine production was observed among the IFNG +874T or A carriers.</p> <p>Conclusion</p> <p>Our results suggest that +874T/A polymorphism was not associated with either susceptibility or severity to leishmaniasis. Despite this, IFNG +874T/A SNP could be involved in the pathogenesis of leishmaniasis by influencing the amount of cytokine released by CL patients, although it could not prevent disease development. On the other hand, it is possible that in ML cases, other potential polymorphic regulatory genes such as TNF-α and IL-10 are also involved thus interfering with IFN-γ secretion.</p