Ancient origins determine global biogeography of hot and cold desert cyanobacteria

Factors governing large-scale spatio-temporal distribution of microorganisms remain unresolved, yet are pivotal to understanding ecosystem value and function. Molecular genetic analyses have focused on the influence of niche and neutral processes in determining spatial patterns without considering the temporal scale. Here, we use temporal phylogenetic analysis calibrated using microfossil data for a globally sampled desert cyanobacterium, Chroococcidiopsis, to investigate spatio-temporal patterns in microbial biogeography and evolution. Multilocus phylogenetic associations were dependent on contemporary climate with no evidence for distance-related patterns. Massively parallel pyrosequencing of environmental samples confirmed that Chroococcidiopsis variants were specific to either hot or cold deserts. Temporally scaled phylogenetic analyses showed no evidence of recent inter-regional gene flow, indicating populations have not shared common ancestry since before the formation of modern continents. These results indicate that global distribution of desert cyanobacteria has not resulted from widespread contemporary dispersal but is an ancient evolutionary legacy. This highlights the importance of considering temporal scales in microbial biogeography

Essential versus accessory aspects of cell death: recommendations of the NCCD 2015

Cells exposed to extreme physicochemical or mechanical stimuli die in an uncontrollable manner, as a result of their immediate structural breakdown. Such an unavoidable variant of cellular demise is generally referred to as ‘accidental cell death’ (ACD). In most settings, however, cell death is initiated by a genetically encoded apparatus, correlating with the fact that its course can be altered by pharmacologic or genetic interventions. ‘Regulated cell death’ (RCD) can occur as part of physiologic programs or can be activated once adaptive responses to perturbations of the extracellular or intracellular microenvironment fail. The biochemical phenomena that accompany RCD may be harnessed to classify it into a few subtypes, which often (but not always) exhibit stereotyped morphologic features. Nonetheless, efficiently inhibiting the processes that are commonly thought to cause RCD, such as the activation of executioner caspases in the course of apoptosis, does not exert true cytoprotective effects in the mammalian system, but simply alters the kinetics of cellular demise as it shifts its morphologic and biochemical correlates. Conversely, bona fide cytoprotection can be achieved by inhibiting the transduction of lethal signals in the early phases of the process, when adaptive responses are still operational. Thus, the mechanisms that truly execute RCD may be less understood, less inhibitable and perhaps more homogeneous than previously thought. Here, the Nomenclature Committee on Cell Death formulates a set of recommendations to help scientists and researchers to discriminate between essential and accessory aspects of cell death

Reconstruction of the eruptive activity on the NE sector of Stromboli volcano: timing of flank eruptions since 15 ka

A multidisciplinary geological and compositional investigation allowed us to reconstruct the occurrence of flank eruptions on the lower NE flank of Stromboli volcano since 15 ka. The oldest flank eruption recognised is Roisa, which occurred at ~15 ka during the Vancori period, and has transitional compositional characteristics between the Vancori and Neostromboli phases. Roisa was followed by the San Vincenzo eruption that took place at ~12 ka during the early stage of Neostromboli period. The eruptive fissure of San Vincenzo gave rise to a large scoria cone located below the village of Stromboli, and generated a lava flow, most of which lies below sea level. Most of the flank eruptions outside the barren Sciara del Fuoco occurred in a short time, between ~9 and 7 ka during the Neostromboli period, when six eruptive events produced scoria cones, spatter ramparts and lava flows. The Neostromboli products belong to a potassic series (KS), and cluster in two differently evolved groups. After an eruptive pause of ~5,000 years, the most recent flank eruption involving the NE sector of the island occurred during the Recent Stromboli period with the formation of the large, highly K calc-alkaline lava flow field, named San Bartolo. The trend of eruptive fissures since 15 ka ranges from N30°E to N55°E, and corresponds to the magma intrusions radiating from the main feeding system of the volcano

Exploring sediment dynamics from source to sink in the Murray-Darling basin using cosmogenic 14C, 10Be, and 26Al

The relatively short half-life of 14C, namely, 5730 years, means that, compared to the other cosmogenic nuclides, it is substantially more sensitive to short term variations in process rates. Both the erosion of steep mountains and the dynamics of sediment transport, storage and recycling occur over timescales that are too short to be detectable by the cosmogenic nuclides that are currently used routinely, namely 10Be and 26Al. In situ 14C on the other hand is ideally suited for these short timescales, and used in combination with 26Al and 10Be, it will allow for rapid fluctuations in process rates and/or the relatively short timescales that characterise sediment transfer and storage to be measured accurately. The above make in situ 14C an important addition to the cosmogenic radionuclide toolkit. We present results of in situ cosmogenic 14C system blank and calibration sample measurements obtained with the recently established ANSTO/UOW in situ 14C extraction system. The 14C extraction scheme follows the design of the University of Cologne, which exploits the phase transformation of quartz to crystobalite to quantitatively extract the carbon as CO2. Offline high-temperature furnace extraction allows a relative rapid sample throughput and can accommodate samples ranging between 0.5 to 4 grams of clean quartz. Following extraction and isolation, the CO2gas is graphitised using a micro-furnace and then measured using AMS similarly to routine small radiocarbon samples. We also present results of 14C, 26Al, and 10Be analyses from sediment samples collected from Australia’s largest river system, the Murray-Darling basin. We use the downstream changes in the ratios of the three radionuclides in samples collected at key locations along the rivers to quantify sediment mixing and sediment storage times in the river basin. Substantial 26Al/10Be ‘burial’ signal is observed in downstream Murray and Darling samples, while in situ 14C suggests complex burial-exposure histories in these samples. This could have implication of interpreting geochemical proxies at the outlet of Murray-Darling Basin for identification of paleoclimate driven sediment sources (i.e. Monsoon vs. Westerlies). © The Author

Erosion rates on subalpine paleosurfaces in the western Mediterranean by in-situ 10Be concentrations in granites: implications for surface processes and long-term landscape evolution in Corsica (France)

A study of erosion rates by in-situ 10Be concentrations in granites of Miocene high-elevation paleosurfaces in Corsica indicates maximum erosion rates between 8 and 24 mm/kyear. The regional distribution of measured erosion rates indicates that the local climatic conditions, namely precipitation, the petrographic composition of granites, and the degree of brittle deformation govern erosion rates. Chemical erosion dominates even at elevations around 2,000 m in presently subalpine climate conditions. Field evidence indicates that erosion operates by continuous dissolution and/or disintegration to grains (grusification). The erosion rates are relatively high with respect to the preservation of inferred Early Miocene landscapes. We infer temporal burial in the Middle Miocene and significantly lower erosion rates in the Neogene until ~3 Ma to explain the preservation of paleosurfaces, in line with fission track data. Valley incision rates that are a magnitude higher than erosion rates on summit surfaces result in relief enhancement and long-term isostatic surface uplift. On the other hand, widening and deepening of valleys by cyclic glaciation progressively destroys the summit surface relics