5,499 research outputs found
Segregation of COPI-rich and anterograde-cargo-rich domains in endoplasmic-reticulum-to-Golgi transport complexes
AbstractMembrane traffic between the endoplasmic reticulum (ER) and the Golgi complex is regulated by two vesicular coat complexes, COPII and COPI. COPII has been implicated in the selective packaging of anterograde cargo into coated transport vesicles budding from the ER [1]. In mammalian cells, these vesicles coalesce to form tubulo-vesicular transport complexes (TCs), which shuttle anterograde cargo from the ER to the Golgi complex [2â4]. In contrast, COPI-coated vesicles are proposed to mediate recycling of proteins from the Golgi complex to the ER [1,5â7]. The binding of COPI to COPII-coated TCs [3,8,9], however, has led to the proposal that COPI binds to TCs and specifically packages recycling proteins into retrograde vesicles for return to the ER [3,9]. To test this hypothesis, we tracked fluorescently tagged COPI and anterograde-transport markers simultaneously in living cells. COPI predominated on TCs shuttling anterograde cargo to the Golgi complex and was rarely observed on structures moving in directions consistent with retrograde transport. Furthermore, a progressive segregation of COPI-rich domains and anterograde-cargo-rich domains was observed in the TCs. This segregation and the directed motility of COPI-containing TCs were inhibited by antibodies that blocked COPI function. These observations, which are consistent with previous biochemical data [2,9], suggest a role for COPI within TCs en route to the Golgi complex. By sequestering retrograde cargo in the anterograde-directed TCs, COPI couples the sorting of ER recycling proteins [10] to the transport of anterograde cargo
Molecular Motor of Double-Walled Carbon Nanotube Driven by Temperature Variation
An elegant formula for coordinates of carbon atoms in a unit cell of a
single-walled nanotube (SWNT) is presented and a new molecular motor of
double-walled carbon nanotube whose inner tube is a long (8,4) SWNT and outer
tube a short (14,8) SWNT is constructed. The interaction between inner an outer
tubes is analytically derived by summing the Lennard-Jones potentials between
atoms in inner and outer tubes. It is proved that the molecular motor in a
thermal bath exhibits a directional motion with the temperature variation of
the bath.Comment: 9 pages, 4 figures, revtex
A Microscopic Mechanism for Muscle's Motion
The SIRM (Stochastic Inclined Rods Model) proposed by H. Matsuura and M.
Nakano can explain the muscle's motion perfectly, but the intermolecular
potential between myosin head and G-actin is too simple and only repulsive
potential is considered. In this paper we study the SIRM with different complex
potential and discuss the effect of the spring on the system. The calculation
results show that the spring, the effective radius of the G-actin and the
intermolecular potential play key roles in the motion. The sliding speed is
about calculated from the model which well agrees with
the experimental data.Comment: 9 pages, 6 figure
The proangiogenic capacity of polymorphonuclear neutrophils delineated by microarray technique and by measurement of neovascularization in wounded skin of CD18-deficient mice
Growing evidence supports the concept that polymorphonuclear neutrophils (PMN) are critically involved in inflammation-mediated angiogenesis which is important for wound healing and repair. We employed an oligonucleotide microarray technique to gain further insight into the molecular mechanisms underlying the proangiogenic potential of human PMN. In addition to 18 known angiogenesis-relevant genes, we detected the expression of 10 novel genes, namely midkine, erb-B2, ets-1, transforming growth factor receptor-beta(2) and -beta(3), thrombospondin, tissue inhibitor of metalloproteinase 2, ephrin A2, ephrin B2 and restin in human PMN freshly isolated from the circulation. Gene expression was confi rmed by the RT-PCR technique. In vivo evidence for the role of PMN in neovascularization was provided by studying neovascularization in a skin model of wound healing using CD18-deficient mice which lack PMN infi ltration to sites of lesion. In CD18-deficient animals, neo- vascularization was found to be signifi cantly compromised when compared with wild- type control animals which showed profound neovascularization within the granulation tissue during the wound healing process. Thus, PMN infiltration seems to facilitate inflammation mediated angiogenesis which may be a consequence of the broad spectrum of proangiogenic factors expressed by these cells. Copyright (c) 2006 S. Karger AG, Basel
Force and Motion Generation of Molecular Motors: A Generic Description
We review the properties of biological motor proteins which move along linear
filaments that are polar and periodic. The physics of the operation of such
motors can be described by simple stochastic models which are coupled to a
chemical reaction. We analyze the essential features of force and motion
generation and discuss the general properties of single motors in the framework
of two-state models. Systems which contain large numbers of motors such as
muscles and flagella motivate the study of many interacting motors within the
framework of simple models. In this case, collective effects can lead to new
types of behaviors such as dynamic instabilities of the steady states and
oscillatory motion.Comment: 29 pages, 9 figure
Greedy Solution of Ill-Posed Problems: Error Bounds and Exact Inversion
The orthogonal matching pursuit (OMP) is an algorithm to solve sparse
approximation problems. Sufficient conditions for exact recovery are known with
and without noise. In this paper we investigate the applicability of the OMP
for the solution of ill-posed inverse problems in general and in particular for
two deconvolution examples from mass spectrometry and digital holography
respectively.
In sparse approximation problems one often has to deal with the problem of
redundancy of a dictionary, i.e. the atoms are not linearly independent.
However, one expects them to be approximatively orthogonal and this is
quantified by the so-called incoherence. This idea cannot be transfered to
ill-posed inverse problems since here the atoms are typically far from
orthogonal: The ill-posedness of the operator causes that the correlation of
two distinct atoms probably gets huge, i.e. that two atoms can look much alike.
Therefore one needs conditions which take the structure of the problem into
account and work without the concept of coherence. In this paper we develop
results for exact recovery of the support of noisy signals. In the two examples
in mass spectrometry and digital holography we show that our results lead to
practically relevant estimates such that one may check a priori if the
experimental setup guarantees exact deconvolution with OMP. Especially in the
example from digital holography our analysis may be regarded as a first step to
calculate the resolution power of droplet holography
Search for the standard model Higgs boson in the H to ZZ to 2l 2nu channel in pp collisions at sqrt(s) = 7 TeV
A search for the standard model Higgs boson in the H to ZZ to 2l 2nu decay
channel, where l = e or mu, in pp collisions at a center-of-mass energy of 7
TeV is presented. The data were collected at the LHC, with the CMS detector,
and correspond to an integrated luminosity of 4.6 inverse femtobarns. No
significant excess is observed above the background expectation, and upper
limits are set on the Higgs boson production cross section. The presence of the
standard model Higgs boson with a mass in the 270-440 GeV range is excluded at
95% confidence level.Comment: Submitted to JHE
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