1,262 research outputs found
Reclamation of Surface-Mined Land
Author Institution: USDA Forest Service, NE Forest Experiment Station, Forest Products Marketing LaboratoryPLASS, WILLIAM T. Reclamation of surface mined lands. Ohio J. Sci. 75(6): 298, 1975
On the testability of coarsening assumptions: a hypothesis test for subgroup independence
Since coarse(ned) data naturally induce set-valued estimators, analysts often assume coarsening at random (CAR) to force them to be single-valued. Focusing on a coarse categorical response variable and a precisely observed categorical covariate, we re-illustrate the impossibility to test CAR and contrast it to another type of coarsening called subgroup independence (SI), using the data of the German Panel Study ``Labour Market and Social Security'' as an example. It turns out that -- depending on the number of subgroups and categories of the response variable -- SI can be point-identifying as CAR, but testable unlike CAR. A main goal of this paper is the construction of the likelihood-ratio test for SI. All issues are similarly investigated for the here proposed generalized versions, gCAR and gSI, thus allowing a more flexible application of this hypothesis test
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GENOME WIDE DNA METHYLATION PROFILING IS PREDICTIVE OF OUTCOME IN JUVENILE MYELOMONOCYTIC LEUKEMIA
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The Hohenpeissenberg aerosol formation experiment (HAFEX): A long-term study including size-resolved aerosol, H2SO4, OH, and monoterpenes measurements
Ambient aerosol size distributions (>3 nm) and OH, H2SO4, and terpene concentrations were measured from April 1998 to August 2000 at a rural continental site in southern Germany. New particle formation (NPF) events were detected on 18% of all days, typically during midday hours under sunny and dry conditions. The number of newly formed particles correlated significantly with solar irradiance and ambient levels of H2SO4. A pronounced anti-correlatation of NPF events with the pre-existing particle surface area was identified in the cold season, often associated with the advection of dry and relatively clean air masses from southerly directions (Alps). Estimates of the particle formation rate based on observations were around 1 cm-3 s-1, being in agreement with the predictions of ternary homogeneous H2SO4-NH3-H2O nucleation within a few orders of magnitude. The experimentally determined nucleation mode particle growth rates were on average 2.6 nm h-1, with a fraction of 0.7 nm h-1 being attributed to the co-condensation of H2SO4-H2O-NH3. The magnitude of nucleation mode particle growth was neither significantly correlated to H2SO4, nor to the observed particle formation rate. Turn-over rate calculations of measured monoterpenes and aromatic hydrocarbons suggest that especially the oxidation products of monoterpenes have the capacity to contribute to the growth of nucleation mode particles. Although a large number of precursor gases, aerosol and meteorological parameters were measured, the ultimate key factors controlling the occurence of NPF events could not be identified
Interplay of Strain Relaxation and Chemically Induced Diffusion Barriers: Nanostructure Formation in 2D Alloys
We study the formation of nanostructures with alternating stripes composed of
bulk-immiscible adsorbates during submonolayer heteroepitaxy. We evaluate the
influence of two mechanisms considered in the literature: (i) strain relaxation
by alternating arrangement of the adsorbate species, and (ii) kinetic
segregation due to chemically induced diffusion barriers. A model ternary
system of two adsorbates with opposite misfit relative to the substrate, and
symmetric binding is investigated by off-lattice as well as lattice kinetic
Monte Carlo simulations. We find that neither of the mechanisms (i) or (ii)
alone can account for known experimental observations. Rather, a combination of
both is needed. We present an off-lattice model which allows for a qualitative
reproduction of stripe patterns as well as island ramification in agreement
with recent experimental observations for CoAg/Ru(0001) [R. Q. Hwang, Phys.
Rev. Lett. 76, 4757 (1996)]. The quantitative dependencies of stripe width and
degree of island ramification on the misfit and interaction strength between
the two adsorbate types are presented. Attempts to capture essential features
in a simplified lattice gas model show that a detailed incorporation of
non-local effects is required.Comment: 24 pages, 12 figure
Aspects of electrostatics in a weak gravitational field
Several features of electrostatics of point charged particles in a weak,
homogeneous, gravitational field are discussed using the Rindler metric to
model the gravitational field. Some previously known results are obtained by
simpler and more transparent procedures and are interpreted in an intuitive
manner. Specifically: (i) We show that the electrostatic potential of a charge
at rest in the Rindler frame is expressible as A_0=(q/l) where l is the affine
parameter distance along the null geodesic from the charge to the field point.
(ii) We obtain the sum of the electrostatic forces exerted by one charge on
another in the Rindler frame and discuss its interpretation. (iii) We show how
a purely electrostatic term in the Rindler frame appears as a radiation term in
the inertial frame. (In part, this arises because charges at rest in a weak
gravitational field possess additional weight due to their electrostatic
energy. This weight is proportional to the acceleration and falls inversely
with distance -- which are the usual characteristics of a radiation field.)
(iv) We also interpret the origin of the radiation reaction term by extending
our approach to include a slowly varying acceleration. Many of these results
might have possible extensions for the case of electrostatics in an arbitrary
static geometry. [Abridged Abstract]Comment: 26 pages; accepted for publication in Gen.Rel.Gra
Rural continental aerosol properties and processes observed during the Hohenpeissenberg Aerosol Characterization Experiment (HAZE2002)
International audienceDetailed investigations of the chemical and microphysical properties of rural continental aerosols were performed during the HAZE2002 experiment, which was conducted in May 2002 at the Meteorological Observatory Hohenpeissenberg (DWD) in Southern Germany. Online measurements included: Size-resolved chemical composition of submicron particles; total particle number concentrations and size distributions over the diameter range of 3 nm to 9 ?m; gas-phase concentration of monoterpenes, CO, O3, OH, and H2SO4. Filter sampling and offline analytical techniques were used to determine: Fine particle mass (PM2.5), organic, elemental and total carbon in PM2.5 (OC2.5, EC2.5, TC2.5), and selected organic compounds (dicarboxylic acids, polycyclic aromatic hydrocarbons, proteins). Overall, the non-refractory components of submicron particles detected by aerosol mass spectrometry (PM1, 6.6±5.4 ?g m?3, arithmetic mean and standard deviation) accounted for ~62% of PM2.5 determined by filter gravimetry (10.6±4.7 ?g m?3). The relative proportions of non-refractory submicron particle components were: (23±39)% ammonium nitrate, (27±23)% ammonium sulfate, and (50±40)% organics (OM1). OM1 was closely correlated with PM1 (r2=0.9) indicating a near-constant ratio of non-refractory organics and inorganics. The average ratio of OM1 to OC2.5 was 2.1±1.4, indicating a high proportion of heteroelements in the organic fraction of the sampled rural aerosol. This is consistent with the high ratio of oxygenated organic aerosol (OOA) over hydrocarbon-like organic aerosol (HOA) inferred from the AMS results (4:1), and also with the high abundance of proteins (~3%) indicating a high proportion of primary biological material (~30%) in PM2.5. This finding was confirmed by low abundance of PAHs (?3) and EC (?3) in PM2.5 and detection of several secondary organic aerosol compounds (dicarboxylic acids) and their precursors (monoterpenes). New particle formation was observed almost every day with particle number concentrations exceeding 104 cm?3 (nighttime background level 1000?2000 cm?3). Closer inspection of two major events indicated that the observed nucleation agrees with ternary H2SO4/H2O/NH3 nucleation and that condensation of both organic and inorganic species contributed to particle growth
Diverse histone modifications on histone 3 lysine 9 and their relation to DNA methylation in specifying gene silencing
<p>Abstract</p> <p>Background</p> <p>Previous studies of individual genes have shown that in a self-enforcing way, dimethylation at histone 3 lysine 9 (dimethyl-H3K9) and DNA methylation cooperate to maintain a repressive mode of inactive genes. Less clear is whether this cooperation is generalized in mammalian genomes, such as mouse genome. Here we use epigenomic tools to simultaneously interrogate chromatin modifications and DNA methylation in a mouse leukemia cell line, L1210.</p> <p>Results</p> <p>Histone modifications on H3K9 and DNA methylation in L1210 were profiled by both global CpG island array and custom mouse promoter array analysis. We used chromatin immunoprecipitation microarray (ChIP-chip) to examine acetyl-H3K9 and dimethyl-H3K9. We found that the relative level of acetyl-H3K9 at different chromatin positions has a wider range of distribution than that of dimethyl-H3K9. We then used differential methylation hybridization (DMH) and the restriction landmark genome scanning (RLGS) to analyze the DNA methylation status of the same targets investigated by ChIP-chip. The results of epigenomic profiling, which have been independently confirmed for individual loci, show an inverse relationship between DNA methylation and histone acetylation in regulating gene silencing. In contrast to the previous notion, dimethyl-H3K9 seems to be less distinct in specifying silencing for the genes tested.</p> <p>Conclusion</p> <p>This study demonstrates in L1210 leukemia cells a diverse relationship between histone modifications and DNA methylation in the maintenance of gene silencing. Acetyl-H3K9 shows an inverse relationship between DNA methylation and histone acetylation in regulating gene silencing as expected. However, dimethyl-H3K9 seems to be less distinct in relation to promoter methylation. Meanwhile, a combination of epigenomic tools is of help in understanding the heterogeneity of epigenetic regulation, which may further our vision accumulated from single-gene studies.</p
Bright single-photon sources in bottom-up tailored nanowires
The ability to achieve near-unity light extraction efficiency is necessary
for a truly deterministic single photon source. The most promising method to
reach such high efficiencies is based on embedding single photon emitters in
tapered photonic waveguides defined by top-down etching techniques. However,
light extraction efficiencies in current top-down approaches are limited by
fabrication imperfections and etching induced defects. The efficiency is
further tempered by randomly positioned off-axis quantum emitters. Here, we
present perfectly positioned single quantum dots on the axis of a tailored
nanowire waveguide using bottom-up growth. In comparison to quantum dots in
nanowires without waveguide, we demonstrate a 24-fold enhancement in the single
photon flux, corresponding to a light extraction efficiency of 42 %. Such high
efficiencies in one-dimensional nanowires are promising to transfer quantum
information over large distances between remote stationary qubits using flying
qubits within the same nanowire p-n junction.Comment: 19 pages, 6 figure
Restriction landmark genomic scanning (RLGS) spot identification by second generation virtual RLGS in multiple genomes with multiple enzyme combinations.
BackgroundRestriction landmark genomic scanning (RLGS) is one of the most successfully applied methods for the identification of aberrant CpG island hypermethylation in cancer, as well as the identification of tissue specific methylation of CpG islands. However, a limitation to the utility of this method has been the ability to assign specific genomic sequences to RLGS spots, a process commonly referred to as "RLGS spot cloning."ResultsWe report the development of a virtual RLGS method (vRLGS) that allows for RLGS spot identification in any sequenced genome and with any enzyme combination. We report significant improvements in predicting DNA fragment migration patterns by incorporating sequence information into the migration models, and demonstrate a median Euclidian distance between actual and predicted spot migration of 0.18 centimeters for the most complex human RLGS pattern. We report the confirmed identification of 795 human and 530 mouse RLGS spots for the most commonly used enzyme combinations. We also developed a method to filter the virtual spots to reduce the number of extra spots seen on a virtual profile for both the mouse and human genomes. We demonstrate use of this filter to simplify spot cloning and to assist in the identification of spots exhibiting tissue-specific methylation.ConclusionThe new vRLGS system reported here is highly robust for the identification of novel RLGS spots. The migration models developed are not specific to the genome being studied or the enzyme combination being used, making this tool broadly applicable. The identification of hundreds of mouse and human RLGS spot loci confirms the strong bias of RLGS studies to focus on CpG islands and provides a valuable resource to rapidly study their methylation
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