Observing the Earth as an exoplanet with LOUPE, the Lunar Observatory for Unresolved Polarimetry of Earth

The detections of small, rocky exoplanets have surged in recent years and will likely continue to do so. To know whether a rocky exoplanet is habitable, we have to characterise its atmosphere and surface. A promising characterisation method for rocky exoplanets is direct detection using spectropolarimetry. This method will be based on single pixel signals, because spatially resolving exoplanets is impossible with current and near-future instruments. Well-tested retrieval algorithms are essential to interpret these single pixel signals in terms of atmospheric composition, cloud and surface coverage. Observations of Earth itself provide the obvious benchmark data for testing such algorithms. The observations should provide signals that are integrated over the Earth's disk, that capture day and night variations, and all phase angles. The Moon is a unique platform from where the Earth can be observed as an exoplanet, undisturbed, all of the time. Here, we present LOUPE, the Lunar Observatory for Unresolved Polarimetry of Earth, a small and robust spectropolarimeter to observe our Earth as an exoplanet.Comment: 14 pages, 3 figures, submitted in special Issue of Planetary and Space Science on Scientific Preparations for Lunar Exploratio

Biomimetic transferable surface for a real time control over wettability and photoerasable writing with water drop lens

We demonstrate a transferable device that can turn wettability of surfaces to sticky or slippy, as per requirement. It is composed of polymeric yarn with a fibrous structure, which can be lifted and placed on any surface to render it the unique wettability properties. We introduce Polyvinylidenefluoride (PVDF) random fiber as biomimetic rose petal surface. When it is decorated with PVDF nanofibers yarns, the random mesh transform from rose petal sticky state into grass leaf slippy state. When it is placed on sticky, hydrophilic metal coin, it converts the surface of the coin to super hydrophobic. Adjustments in the yarn system, like interyarn spacing, can be done in real time to influence its wettability, which is a unique feature. Next, we load the polymer with a photochromic compound for chemical restructuring. It affects the sliding angle of water drop and makes the fibers optically active. We also demonstrate a “water droplets lens” concept that enables erasable writing on photochromic rose petal sticky fibrous surface. The droplet on a highly hydrophobic surface acts as a ball lens to concentrate light onto a hot spot; thereby we demonstrate UV light writing with water lenses and visible light erasing

Biophysical Assessment of Single Cell Cytotoxicity: Diesel Exhaust Particle-Treated Human Aortic Endothelial Cells

Exposure to diesel exhaust particles (DEPs), a major source of traffic-related air pollution, has become a serious health concern due to its adverse influences on human health including cardiovascular and respiratory disorders. To elucidate the relationship between biophysical properties (cell topography, cytoskeleton organizations, and cell mechanics) and functions of endothelial cells exposed to DEPs, atomic force microscope (AFM) was applied to analyze the toxic effects of DEPs on a model cell line from human aortic endothelial cells (HAECs). Fluorescence microscopy and flow cytometry were also applied to further explore DEP-induced cytotoxicity in HAECs. Results revealed that DEPs could negatively impair cell viability and alter membrane nanostructures and cytoskeleton components in a dosage- and a time-dependent manner; and analyses suggested that DEPs-induced hyperpolarization in HAECs appeared in a time-dependent manner, implying DEP treatment would lead to vasodilation, which could be supported by down-regulation of cell biophysical properties (e.g., cell elasticity). These findings are consistent with the conclusion that DEP exposure triggers important biochemical and biophysical changes that would negatively impact the pathological development of cardiovascular diseases. For example, DEP intervention would be one cause of vasodilation, which will expand understanding of biophysical aspects associated with DEP cytotoxicity in HAECs

Detection of polyol accumulation in a new ovarian carcinoma cell line, CABA I: a1H NMR study

Ovarian carcinomas represent a major form of gynaecological malignancies, whose treatment consists mainly of surgery and chemotherapy. Besides the difficulty of prognosis, therapy of ovarian carcinomas has reached scarce improvement, as a consequence of lack of efficacy and development of drug-resistance. The need of different biochemical and functional parameters has grown, in order to obtain a larger view on processes of biological and clinical significance. In this paper we report novel metabolic features detected in a series of different human ovary carcinoma lines, by 1H NMR spectroscopy of intact cells and their extracts. Most importantly, a new ovarian adenocarcinoma line CABA I, showed strong signals in the spectral region between 3.5 and 4.0 p.p.m., assigned for the first time to the polyol sorbitol (39±11 nmol/106 cells). 13C NMR analyses of these cells incubated with [1-13C]-D-glucose demonstrated labelled-sorbitol formation. The other ovarian carcinoma cell lines (OVCAR-3, IGROV 1, SK-OV-3 and OVCA432), showed, in the same spectral region, intense resonances from other metabolites: glutathione (up to 30 nmol/106 cells) and myo-inositol (up to 50 nmol/106 cells). Biochemical and biological functions are suggested for these compounds in human ovarian carcinoma cells, especially in relation to their possible role in cell detoxification mechanisms during tumour progression

Mesoscale to Submesoscale Wavenumber Spectra in Drake Passage

This study discusses the upper-ocean (0-200 m) horizontal wavenumber spectra in the Drake Passage from 13 yr of shipboard ADCP measurements, altimeter data, and a high-resolution numerical simulation. At scales between 10 and 200 km, the ADCP kinetic energy spectra approximately follow a k-3 power law. The observed flows are more energetic at the surface, but the shape of the kinetic energy spectra is independent of depth. These characteristics resemble predictions of isotropic interior quasigeostrophic turbulence. The ratio of across-track to along-track kinetic energy spectra, however, significantly departs from the expectation of isotropic interior quasigeostrophic turbulence. The inconsistency is dramatic at scales smaller than 40 km. A Helmholtz decomposition of the ADCP spectra and analyses of synthetic and numerical model data show that horizontally divergent, ageostrophic flows account for the discrepancy between the observed spectra and predictions of isotropic interior quasigeostrophic turbulence. In Drake Passage, ageostrophic motions appear to be dominated by inertia-gravity waves and account for about half of the near-surface kinetic energy at scales between 10 and 40 km. Model results indicate that ageostrophic flows imprint on the sea surface, accounting for about half of the sea surface height variance between 10 and 40 km

Upregulated Heat Shock Proteins After Hyperthermic Chemotherapy Point to Induced Cell Survival Mechanisms in Affected Tumor Cells From Peritoneal Carcinomatosis

In patients with peritoneal carcinomatosis cytoreductive surgery combined with hyperthermic intraperitoneal chemotherapy (HIPEC) represents a promising treatment strategy. Here, we studied the role of hyperthermic chemotherapy on heat shock protein (HSP) expression and induction of tumor cell death and survival. HSP27, HSP70, and HSP90 combined with effects on tumor cell proliferation and chemosensitivity were analyzed in human colon cancer. Hyperthermic chemotherapy resulted in significant HSP27/HSP70 and HSP90 gene/protein overexpression in analyzed HT-29/SW480/SW620 colon cancer cells and peritoneal metastases from patients displaying amplified expression of proliferation markers, proliferating cell nuclear antigen and antiapoptotic protein Bcl-xL. Moreover, functionally increased chemoresistance against 5-fluorouracil/mitomycin C and oxaliplatin after hyperthermic chemotherapy points to induced survival mechanisms in cancer cells. In conclusion, the results indicate that intracellular HSP-associated antiapoptotic and proliferative effects after hyperthermic chemotherapy negatively influence beneficial effects of hyperthermic chemotherapy-induced cell death. Therefore, blocking HSPs could be a promising strategy to further improve the rate of tumor cell death and outcome of patients undergoing HIPEC therapy