419 research outputs found
The Feather Mite Genus Proctophyllodes (\u3ci\u3eSarcoptiformes: Proctopbyllodidae\u3c/i\u3e)
A systematic revision is presented for the genus Proctophyllodes. Data on the bionomics, morphology, and host-parasite relationships are included
Species of Bursaphelenchus Fuchs, 1937 (Nematoda: Parasitaphelenchidae) and other nematode genera associated with insects from Pinus pinaster in Portugal
Insects associated with maritime pine, Pinus pinaster, in Portugal were collected
and screened for the presence of Bursaphelenchus species. Nematodes were
identified using Internal Transcribed Spacers-Restriction Fragment Length
Polymorphism (ITS-RFLP) analysis of dauer juveniles and morphological
identification of adults that developed from dauer juveniles on fungal cultures
or on cultures in pine wood segments at 26 C. Several associations are
described: Bursaphelenchus teratospicularis and Bursaphelenchus sexdentati are
associated with Orthotomicus erosus; Bursaphelenchus tusciae, B. sexdentati and/or
Bursaphelenchus pinophilus with Hylurgus ligniperda and Bursaphelenchus hellenicus
with Tomicus piniperda, Ips sexdentatus and H. ligniperda. An unidentified
Bursaphelenchus species is vectored by Hylobius sp. The previously reported
association of Bursaphelenchus xylophilus with Monochamus galloprovincialis
was confirmed. The association of Bursaphelenchus leoni with Pityogenes sp. is
not definitively established and needs further studies for clarification.
Other nematode genera besides Bursaphelenchus were found to be associated
with the insects sampled, including two different species of Ektaphelenchus, Parasitorhabditis
sp., Parasitaphelenchus sp., Contortylenchus sp. and other unidentified
nematodes. The Ektaphelenchus species found in O. erosus is morphologically
similar to B. teratospicularis found in the same insect; adults of both the species
are found in cocoon-like structures under the elytra of the insects.
Introduction
Approximately one third of the nematodes belonging to
the order Aphelenchida Siddiqi, 1980 are associated with
insects (Poinar, 1983). These nematodes establish a variety
of associations with the insects, which may be
described as commensalism, e.g. phoresy (to the benefit
of the nematode but not affecting the insect), mutualism
(both the organisms benefit) or parasitism (nematodes
benefit at the expense of the insect) (Giblin-Davis,
2004).
Most Bursaphelenchus Fuchs, 1937 species are mycetophagous,
feeding on fungi in the galleries of bark beetles
and thu
Source localization in complex listening situations: Selection of binaural cues based on interaural coherence
Acute-Phase CD8 T cell responses that select for escape variants are needed to control live attenuated simian immunodeficiency virus
The overall CD8 T cell response to human/simian immunodeficiency virus (HIV/SIV) targets a collection of discrete epitope specificities. Some of these epitope-specific CD8 T cells emerge in the weeks and months following infection and rapidly select forsequence variants, whereas other CD8 T cell responses develop during the chronic infection phase and rarely select for sequence variants. In this study, we tested the hypothesis that acute-phase CD8 T cell responses that do not rapidly select for escapevariants are unable to control viral replication in vivo as well as those that do rapidly select for escape variants. We created a derivative of live attenuated SIV (SIVmac239Înef) in which we ablated five epitopes that elicit early CD8 T cell responses and rapidly accumulate sequence variants in SIVmac239-infected Mauritian cynomolgus macaques (MCMs) that are homozygous for the M3 major histocompatibility complex (MHC) haplotype. This live attenuated SIV variant was called m3KOÎnef. Viremia was significantly higher in M3 homozygous MCMs infected withm3KOÎnef than in either MHC-mismatched MCMs infected with m3KOÎ nef or MCMs infected with SIVmac239Înef. Three CD8 T cell responses, including two that do not rapidly select for escape variants, predominated during earlym3KOÎnef infection in the M3 homozygous MCMs, but these animals were unable to control viral replication. These re-sults provide evidence that acute-phase CD8 T cell responses that have the potential to rapidly select for escape variants in the early phase ofinfection are needed to establish viral control in vivo
Impaired Wound Healing in Mouse Models of Diabetes Is Mediated by TNF-α Dysregulation and Associated With Enhanced Activation of Forkhead Box O1 (FOXO1)
Aims/hypothesis
The role of TNF-α in impaired wound healing in diabetes was examined by focusing on fibroblasts. Methods
Small excisional wounds were created in the db/db mice model of type 2 diabetes and normoglycaemic littermates, and in a streptozotocin-induced type 1 diabetes mouse model and control mice. Fibroblast apoptosis was measured by the TUNEL assay, proliferation by detection of proliferating cell nuclear antigen, and forkhead box O1 (FOXO1) activity by DNA binding and nuclear translocation. TNF-α was specifically inhibited by pegsunercept. Results
Diabetic wounds had increased TNF-α, fibroblast apoptosis, caspase-3/7 activity and activation of the pro-apoptotic transcription factor FOXO1, and decreased proliferating cell nuclear antigen positive fibroblasts (pâ\u3câ0.05). TNF-α inhibition improved healing in the diabetic mice and increased fibroblast density. This may be explained by a decrease in fibroblast apoptosis and increased proliferation when TNF-α was blocked (pâ \u3câ0.05). Although decreased fibroblast proliferation and enhanced FOXO1 activity were investigated in type 2 diabetes, they may also be implicated in type 1 diabetes. In vitro, TNF-α enhanced mRNA levels of gene sets related to apoptosis and Akt and p53 but not mitochondrial or cell-cycle pathways. FOXO1 small interfering RNA reduced gene sets that regulate apoptosis, Akt, mitochondrial and cell-cycle pathways. TNF-α also increased genes involved in inflammation, cytokine, Toll-like receptor and nuclear factor-kB pathways, which were significantly reduced by FOXO1 knockdown. Conclusions/interpretation
These studies indicate that TNF-α dysregulation in diabetic wounds impairs healing, which may involve enhanced fibroblast apoptosis and decreased proliferation. In vitro, TNF-α induced gene sets through FOXO1 that regulate a number of pathways that could influence inflammation and apoptosis
Species of Bursaphelenchus Fuchs, 1937 (Nematoda: Parasitaphelenchidae) and other nematode genera associated with insects from Pinus pinaster in Portugal
The bowfin genome illuminates the developmental evolution of ray-finned fishes.
The bowfin (Amia calva) is a ray-finned fish that possesses a unique suite of ancestral and derived phenotypes, which are key to understanding vertebrate evolution. The phylogenetic position of bowfin as a representative of neopterygian fishes, its archetypical body plan and its unduplicated and slowly evolving genome make bowfin a central species for the genomic exploration of ray-finned fishes. Here we present a chromosome-level genome assembly for bowfin that enables gene-order analyses, settling long-debated neopterygian phylogenetic relationships. We examine chromatin accessibility and gene expression through bowfin development to investigate the evolution of immune, scale, respiratory and fin skeletal systems and identify hundreds of gene-regulatory loci conserved across vertebrates. These resources connect developmental evolution among bony fishes, further highlighting the bowfin's importance for illuminating vertebrate biology and diversity in the genomic era
Surmounting a PCR challenge using a Contradictory matrix from the Theory of Inventive Problem Solving (TRIZ)
Chromosomal-level assembly of the Asian Seabass genome using long sequence reads and multi-layered scaffolding
We report here the ~670 Mb genome assembly of the Asian seabass (Lates calcarifer), a tropical marine teleost. We used long-read sequencing augmented by transcriptomics, optical and genetic mapping along with shared synteny from closely related fish species to derive a chromosome-level assembly with a contig N50 size over 1 Mb and scaffold N50 size over 25 Mb that span ~90% of the genome. The population structure of L. calcarifer species complex was analyzed by re-sequencing 61 individuals representing various regions across the species' native range. SNP analyses identified high levels of genetic diversity and confirmed earlier indications of a population stratification comprising three clades with signs of admixture apparent in the South-East Asian population. The quality of the Asian seabass genome assembly far exceeds that of any other fish species, and will serve as a new standard for fish genomics
Utilizing associational resistance for biocontrol: impacted by temperature, supported by indirect defence
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