Preservation of diversity and oenological properties of wine yeasts during long-term laboratory maintenance: A study of strains of a century-old Tokaj wine yeast collection

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Spermaantitestek kimutatása donor inszeminált nőknél

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Lack of Association between C385A Functional Polymorphism of the Fatty Acid Amide Hydrolase Gene and Polycystic Ovary Syndrome.

The endocannabinoid system contributes to the regulation of appetite, food intake and energy balance. Fatty acid amide hydrolase is responsible for degradating anandamide, a key messenger of the endocannabinoid system. C385A is a common, functionally active genetic polymorphism of the gene encoding fatty acid amide hydrolase and has been associated with overweight and obesity. Our aim was to establish whether single nucleotide polymorphism C385A has an association with polycystic ovary syndrome or its clinical features.A monocentric pilot study was performed on 63 patients with polycystic ovary syndrome and 67 healthy control subjects. Anthropometric parameters and laboratory data were acquired from subjects. The alleles of the polymorphism were detected using polymerase chain reaction and subsequent cleavage by Eco130I (StyI) restriction endonuclease verified by direct DNA sequencing.No difference was found in minor allele frequency between patient and control groups. Those patients, carrying the C385A polymorphism were associated with higher free thyroxine hormone levels. In the control group, carriers of the polymorphism had significantly lower insulin levels.Our data indicate that the C385A polymorphism of the fatty acid amide hydrolase gene is not a genetic susceptibility factor for the development of polycystic ovary syndrome. However, the polymorphism might have a role in influencing the synthesis or metabolism of different hormones including thyroxin and insulin. © J. A. Barth Verlag in Georg Thieme Verlag KG Stuttgart · New York

Lack of association between serotonin transporter 5-HTT gene polymorphism and endometriosis in an Italian patient population

Background: The aim of this study was to determine whether the serotonin transporter gene (5-HTT), a key component in the control of the serotonergic system, is associated with endometriosis in an Italian population. Findings. A case-control study, comprising 137 Italian patients with surgically confirmed endometriosis and 120 healthy controls, was carried out. 5-HTT genotypes (LL, SL and SS) were obtained by polymerase chain reaction and gel electrophoresis analysis. We found no overall difference in genotypic and allelic distributions of the 5-HTT gene between cases and controls. Conclusions: Our results suggest that the 5-HTT L/S promoter polymorphism is not associated with susceptibility to endometriosis in the studied Italian patients. © 2014 Megiorni et al.; licensee BioMed Central Ltd

Chronic pelvic pain: gynaecological and non-gynaecological causes and considerations for nursing care

Chronic pelvic pain (CPP) is one of the most difficult problems encountered by health professionals, and it is a very common gynaecological complaint in women of reproductive age. Any structure in the abdomen and/or pelvis could have a role in aetiology of CPP. The aetiology, however, is often unclear and the origin seems can be multifactorial. A multidisciplinary team can offer simultaneous assessment and management of somatic, behavioural and psychosocial components of the pain, and the nurse as an integral member of the healthcare team has an important role to play in ensuring effective care provision. The purpose of this review is to present comprehensive and contemporary information about the gynaecological and non-gynaecological causes of CPP alongside an examination of the role of the nurse in caring for women who experience this problem