Développement Testiculaire chez Poisson Combattant (Betta splendens), variété de Demi-lune, Subis 35 jours de Différents Régimes de Photopériodes

Fighting fish (Betta splendens) is one of the fresh water ornamental fish with highly commercial value. This fish has a distinctive morphological shape which is aesthetic and is easy to maintain. Demand for this fish increased leading to opportunities for cultivation. Quality and quantity of fries were constantly needed, and this could be achieved by manipulating reproduction. Photoperiod regimes could play a role to do this. The purpose of this study was to determine the effect of photoperiods on the testis development of halfmoon strains of fighting fish.             A completely randomized design was run to test 3 treatments in quintuplicates. The fish were reared under treatment P1 (10 hours light L and 14 hours dark D), treatment P2 (14 hours L and 10 hours D) and treatment P0 (12 hours L and 12 hours D). The fish were maintained for 35-d, in Aquatic Resources Laboratory of FPIK Unsoed. The results showed that treatment P2 was significantly different (P<0.05), which the highest spermatogenesis stage (54.78 ± 16.64%) was obtained. The study concluded that male fighting fish under P2 photoperiod could accelerate testicular maturation

Potensi Lestari dan Beberapa Aspek Biologi Ikan Pari (Dasyatis sp.) sebagai Tinjauan Status Perikanan Tangkap di Laut Jawa

Ikan Pari merupakan komoditas ikan demersal di perairan Indonesia yang semakin menurun produksi tangkapannya. Tujuan dari penelitian ini adalah mengetahui potensi lestari dan beberapa aspek biologi ikan Pari dengan menggunakan indikator Maximum Sustainable Yield (MSY) sebagai status penangkapan di laut Jawa. Data hasil tangkapan dan upaya diambil dari data produksi tahun 2006-2015 dan data primer untuk mengetahui beberapa aspek biologinya diambil sempel di TPI pelabuhan Cirebon dan Tegal Data spesifikasi alat tangkap ikan Pari diperoleh dari wawancara nelayan. Data hasil tangkapan di analisis menggunakan Metode Surplus Produksi (Schaefer) dan aspek biologi ikan pari yang diamati antara lain : nisbah kelamin dan distribusi ukuran panjang ikan Pari. Hasil penelitian menunjukkan nilai MSY ikan Pari sebesar 894,275 ton/tahun dan fMSY sebesar 34,716 kapal/tahun. Nisbah kelamin ikan Pari Jantan dengan ikan Pari betina masih seimbang, yaitu 1 : 1,34 dan ukuran frekuensi panjang masuk kedalam kategori ikan yang belum matang gonad dan ikan yang pertama kali matang gonad, yaitu berkisar antara panjang 16 – 22 cm baik ikan Pari jantan maupun betina. Berdasarkan hasil penelitian dapat disimpulkan bahwa penangkapan ikan Pari di laut Jawa masih dalam kondisi lestari dengan kondisi ikan masih muda

PRESERVASI BEKU SPERMATOZOA IKAN CUPANG (Betta splendens) STRAIN HALF-MOON DALAM MADU DAN NACL FISIOLOGIS

A study entitled “Chilled Preservation of Spermatozoa Half-moon Strain Fighting Fish (Betta spendens) in Honey and Physiological NaCl”, was conducted to determine preserved spermatozoa motility and viability of Fighting Fish in honey and NaCl solutions, under chilling temperature (-250C). The study applied Completely Randomized Design (CRD) to examine three treatments, i.e aquadest, 0.09% NaCl and honey extenders, with quantuplicates. Data, being spermatozoa motility (%) and viability (time in second), were F-tested (ANOVA) and followed by LSD test (P<0.01). After storage of 7 days, the result showed that spermatozoa motility did not differ between treatments, however the viability of spermatozoa were significantly different (P<0.01). The highest viability was observed in sperm stored in extender Honey averaging 368.90±102.16 seconds, compared to the 0.09% NaCl extender (81.79±8.54 seconds), and aquadest (187.90±35.36 seconds). It was concluded that the Honey, NaCl 0.09% and aquadest as extenders could be used as preservation solution fighting fish spermatozoa. The best viability of spermatozoa was observed in honey extender.   &nbsp

Aspek Reproduksi Spesies Asli Ikan Palung (Hampala macrolepidota C.V. 1823) di Waduk PB. Soedirman Banjarnegara, Jawa Tengah

Penelitian ini berjudul “Aspek Reproduksi Spesies Asli Ikan Palung (Hampala macrolepidota C.V. 1823) Di Waduk PB. Soedirman Banjarnegara, Jawa Tengah” telah dilakukan. Penelitian ini bertujuan untuk mengetahui aspek reproduksi meliputi rasio kelamin, indeks gonado somatik, ukuran pertamakali matang gonad, fekunditas, dan diameter telur ikan palung di Waduk. Pengambilan sampel yaitu pada bulan November 2020 - November 2021, alat tangkap penelitian ini adalah gill net panjang 30 m, lebar 3 m. Jumlah ikan hasil tangkapan sebanyak 60 ekor dengan jantan 54 ekor dan betina 6 ekor, hasil rasio kelamin ikan palung 9 : 1. IGS jantan (0,01 – 1,54%) lebih rendah dibanding betina (0,02 – 13,91%). Ukuran pertamakali matang gonad jantan (24,95 cm) lebih pendek dibanding  betina (39,54 cm). Fekunditas bulan April 2021 ikan berukuran panjang 20,7 cm, berat 142 g yaitu 30.612 butir dan bulan November 2021 ikan berukuran panjang 44 cm, berat 1335 gr yaitu 60.036 butir. Diameter telur bulan April 2021 yaitu 0,715 – 1,128 mm dan bulan November 2021 yaitu 0,542 – 1,059 mm

POLIPLOIDISASI IKAN NILEM (Osteochilus hasselti Valenciennes, 1842) DENGAN KEJUT DINGIN 40 CPOLYPLOIDIZATION ON SHARK MINNOW (Osteochilus hasselti Valenciennes, 1842) BY COLD SHOCK 40 C

Penelitian ini bertujuan untuk mengetahui tingkat keberhasilan poliploidisasi, yakni triploidisasi dan tetraploidisasi  ikan nilem (Osteochilus hasselti Valenciennes, 1842) menggunakan kejut dingin 40C. Variabel utama adalah persentase penetasan telur, kelangsungan hidup, abnormalitas dan dimensi sel darah merah  benih ikan nilem serta kualitas air sebagai variabel pendukungnya. Penelitian dilakukan dengan tujuh perlakuan dan empat ulangan. Telur dan milt encer segar difertilisasi, pada 5, 20 atau 25 menit setelah fertilisasi dikejut temperatur dingin 40C selama 20 atau 30 menit. Persentase penetasan dihitung 24 jam setelah fertilisasi. Abnormalitas, kelangsungan hidup dan penentuan tingkat ploidi dengan pengukuran dimensi sel darah merah dihitung pada benih ikan nilem umur 60 hari. Hasil penelitian menunjukkan bahwa abnormalitas, kelangsungan hidup dan dimensi sel darah merah benih ikan nilem berbeda sangat nyata (P<0,01), sedangkan penetasan telur dan laju pertumbuhan spesifiknya tidak berbeda nyata (P>0,05). Hasil uji lanjut membuktikan bahwa perlakuan 5 atau 20 menit pasca fertilisasi  dan durasi kejut 30 menit memberikan hasil yang secara nyata paling baik dibandingkan perlakuan lain. Lama kejut nampak efektif dari penelitian ini. Dengan demikian, kejut temperatur dingin 40 C pada poliploidisasi ikan nilem ini siap diterapkan pada bidang akuakultur.  Kata Kunci : Triploidisasi, Tetraploidisasi, Ikan Nilem, Kejut Dingi

Aspek Reproduksi Spesies Asli Ikan Palung (Hampala macrolepidota C.V. 1823) Di Waduk PB. Soedirman Banjarnegara, Jawa Tengah

Penelitian ini berjudul “Aspek Reproduksi Spesies Asli Ikan Palung (Hampala macrolepidota C.V.1823) Di Waduk PB. Soedirman Banjarnegara, Jawa Tengah” telah dilakukan. Penelitian inibertujuan untuk mengetahui aspek reproduksi meliputi rasio kelamin, indeks gonado somatik, ukuranpertamakali matang gonad, fekunditas, dan diameter telur ikan palung di Waduk. Pengambilansampel yaitu pada bulan November 2020 - November 2021, alat tangkap penelitian ini adalah gill netpanjang 30 m, lebar 3 m. Jumlah ikan hasil tangkapan sebanyak 60 ekor dengan jantan 54 ekor danbetina 6 ekor, hasil rasio kelamin ikan palung 9 : 1. IGS jantan (0,01 – 1,54%) lebih rendah dibandingbetina (0,02 – 13,91%). Ukuran pertamakali matang gonad jantan (24,95 cm) lebih pendek dibandingbetina (39,54 cm). Fekunditas bulan April 2021 ikan berukuran panjang 20,7 cm, berat 142 g yaitu30.612 butir dan bulan November 2021 ikan berukuran panjang 44 cm, berat 1335 gr yaitu 60.036butir. Diameter telur bulan April 2021 yaitu 0,715 – 1,128 mm dan bulan November 2021 yaitu 0,542– 1,059 mm

Pengaruh Fotoperiod Berbeda Terhadap Perkembangan Ovarium Ikan Cupang (Betta splendens)

Ikan cupang (Betta splendens) merupakan ikan hias dengan nilai komersial yang tinggi dan memiliki keistimewaan morfologi dengan nilai estetika. Pemintaan ikan cupang terus meningkat sehingga mendorong peluang budidaya ikan hias air tawar dengan mengembangkan teknologi dalam proses reproduksi. Cara memenuhi permintaan pasar ikan cupang dengan menggunakan cara manipulasi reproduksi salah satunya dengan metode fotoperiod. Tujuan penelitian mengetahui pengaruh perlakuaan fotoperiod berbeda terhadap perkembangan ovarium ikan cupang. Rancangan penelitian menggunakan rancangan acak lengkap dengan 3 perlakukan dan 5 ulangan. Ikan cupang yang di pelihara dengan perlakukan A (8 jam terang dan 16 jam gelap), perlakuan B (16 Jam terang dan 8 jam gelap) dan perlakuan C (12 jam terang dan 12 jam gelap). Lama pemeliharaan ikan cupang selama 40 hari. Kegiatan penelitian dilaksanakan di Laboratorium Sumberdaya Akuatik FPIK Unsoed. Hasil penelitian Anova menunjukkan perbedaan nyata (P<0,05) pada perlakuan 16L:8D, dengan tahapan oogenesis tertinggi (27,56 ±7,39%), Kesimpulan dari penelitian bahwa ikan cupang betina dengan perlakuan fotoperiod (16L:8D) dapat mempercepat proses kematangan ovarium pada ikan cupangBetta fish (Betta splendens) is an ornamental fish with high commercial value and has distinctive morphology with aesthetic value. The demand for Betta fish continues to increase, thereby encouraging opportunities for cultivating freshwater ornamental fish by developing technology in the reproduction process. One way to meet market demand for Betta fish is by using reproductive manipulation methods, one of which is the photoperiod method. The aim of the research was to determine the effect of different photoperiod treatments on the development of Betta fish ovaries. The research design used a completely randomized design with 3 treatments and 5 replications. Betta fish were kept in treatment A (8 hours light and 16 hours dark), treatment B (16 hours light and 8 hours dark) and treatment C (12 hours light and 12 hours dark). The length of time for keeping betta fish is 40 days. Research activities were carried out at the Aquatic Resources Laboratory of FPIK Unsoed. The results of the Anova research showed a significant difference (P<0.05) in the 16L:8D treatment, with the highest oogenesis stage (27.56 ± 7.39%). The conclusion from the research was that female Betta fish with photoperiod treatment (16L:8D) could accelerate the process of ovarian maturity in betta fish

The Effect of Different Photoperiods on The Oogenesis of Fighting Fish (Betta Splendens)

The demand for fighting fish Betta splendens continues to increase every year, this encourages farmers to develop technology in the production process. Accelerating reproduction is one solution to support production demand. One technology prior to this is photoperiod. The aim of this research was to evaluate different photoperiod treatments on the stages of betta fish ovarian development. The method used is completely randomized design (CRD) to test 3 different treatments in quintuplicates. Treatment P1 (10-h Light L :14-h Dark D), P2 (14-h L :10-h Dark D), and P3 (Control, ambient lights). Observation of the oogenesis stages of betta fish was carried out through histological preparations classic and calculating the proportions of oogenesis stages. Data were then analyzed statistically (ANOVA) with 0.05 level. The research results showed that treatment P2 was significantly different from P1 and P3, due to domination of previtellogenic oocytes (22.05%), oocyte exogenous vitellogenin (22.89%), and hydrated vitellogenin oocytes (25.24%). Then followed by treatment P1 (10L; 14D) presented previtellogenic oocytes ​​(20.86%), exogenous vitellogenin oocyte (20.17%), and hydrated vitellogenin oocytes (21.82%). In Control, ovaria contained previtellogenic oocytes (19.43%), exogenous vitellogenin oocytes (18.79%), and hydrated vitellogenin oocytes (19.21%). This indicated that under such photoperiod treatments fish kept on adept to develop their ovaria. Moreover, fish matured their ovaria and were apt to spawn

Reproductive Cycle and Plasma Levels of Sex Steroids in Female Eurasian Perch Perca Fluviatilis

From April 1995 to April 1996, the annual reproductive cycle of the Eurasian perch Perca fluviatilis was studied at the Fishfarming Lindre Center (Moselle, France). At monthly intervals (at intervals of 10 days during the periovulatory period), 5 females were caught and dissected. From sampled organs, the gonado-, hepato- and viscerosomatic indexes (GSI, HSI, VSI) were calculated, oocyte diameters (OD) and the plasma levels of testosterone (T), 17P-estradiol (E2), 17,20P-dihydroxy-4-pregnen-3-one (17,2Op-P) and protein-phosphorus (PPP) were measured. After the sexual resting period observed from May to August (GSI \u3c 1 %, OD \u3c 200 urn, VSI = 4-6 %), oogenesis began in September when the water temperature decreased from 26.4 to 14.1 “C. The GSI increased progressively until mid March (15 %), then rapidly until spawning (25 %, OD = 850 urn) which occurred in April (14- 15 “C). The plasma levels of T, E,, 17,2Op-P and PPP were low during the sexual resting period. E, and PPP levels increased significantly at the onset of the oogenesis in September, then the E, level raised abruptly in November (3-4 ng mL-‘). In December, the T level increased rapidly to 15-20 ng . mL-’ The testosterone, E, and PPP levels remained very high until spawning, indicating the existence of active vitellogenesis. The highest HSI (2.1-2.2 %) recorded in winter confirmed this. During the periovulatory period, a peak of E, (4 ng . mL-‘) appeared, whereas T level diminished. In this study, 17,2Op-P levels remained low (0.2-0.6 ng mL-‘) and relatively constant. No 17,2Op-P peak was observed during the periovulatory period. Sampling at 10 day intervals was probably inadequate to specify the hormonal variations related to the final oocyte maturation and the ovulation. 0 Ifremer-Elsevier, Paris

Reproductive Cycle and Plasma Sex Steroid Profiles in Male Eurasian Perch Perca Fluviatilis

From April 1995 to April 1996, the annual reproductive cycle of male Eurasian perch Perca fluviatilis was studied at the Lindre Center (Moselle, France). At monthly intervals, five males (mean body weight of 133 ± 43 g and total length of 175 ± 9 mm) were caught. From sampled organs, the gonadosomatic (GSI), hepatosomatic (HSI) and viscerosomatic indexes (VSI) were calculated and plasma testosterone (T) and 11-ketotestosterone (11KT) levels were measured. After the spawning period in mid-April, GSI and HSI dropped and VSI increased to 3.8 ± 0.1 %. In September, GSI reached its maximum (8.5 ± 1.8 %). At this time, spermatocytes, spermatids and spermatozoa were abundant, whereas in June only spermatogonia were observed. During winter, GSI was stable at approximately 5 %, HSI reached its maximum (1.9 ± 0.3 %) and VSI was low (2.6 ± 0.2 %). From April to November 1995, plasma T and 11KT concentrations were low (\u3c 0.5 ng·mL–1). Plasma T levels increased significantly in December and reached peak levels (12.3 ± 2.1 ng·mL–1) in January, then decreased in February and increased again until spawning in April (6.8 ± 2.1 ng·mL–1). This second elevation could be related to the beginning of a new spermatogenic cycle. Plasma levels of 11KT increased significantly from October to February 1996 (4.9 ± 1.1 ng·mL–1). From February to the spawning period (April 1996), plasma 11KT decreased significantly, but values were significantly higher than those measured in fall. Males were spermiating from January to spawning in April. © 2000 Ifremer/Cnrs/Inra/Ird/Cemagref/Éditions scientifiques et médicales Elsevier SA