DNA identification of kayu kuning (yellow-fruited moonseed) from East Kalimantan, Indonesia

Kalimantan is an island with an abundance of kayu Kuning (yellow-fruited moonseed). Kayu kuning is used for three different plant species, namely Arcangelisia flava (L.) Merr., Fibraurea tinctoria Lour., and Coscinium fenestratum (Gaertn.) Colebr. Therefore, it creates confusion and may cause improper use. It has properties such as anti-diabetic, antiplasmodial, antidiarrheal, hepatitis, and antidote. The study uses the DNA barcode technique to identify kayu kuning (yellow-fruited moonseed) from East Kalimantan, Indonesia. The genomic DNA of kayu kuning (yellow-fruited moonseed) was extracted, and ITS primers were used for identification using polymerase chain reaction (PCR). It was compared with Arcangelisia flava, Fibraurea tinctoria, and Coscinium fenestratum as a phylogenetic tree. DNA sequence alignment of ITS and phylogenetic reconstruction revealed that kayu kuning (yellow-fruited moonseed) from East Kalimantan was closely related to A. flava. The kayu kuning (yellow-fruited moonseed) had 94.16% of sequence similarity with A. flava according to the ITS1 barcode

Identification and Determination of Berberine from Arcangelisia Flava, East Borneo

Berberine is a compound that has various benefits but also has dangerous toxic effects. In Indonesia, the Regulation of the Head of the Food and Drug Supervisory Agency No. 10 of 2014 concerning the Prohibition of Producing and Circulating Traditional Medicines and Health Supplements Containing Coptis Sp, Berberis Sp, Mahonia Sp, Chelidonium Majus, Phellodendron Sp, Arcangelica Flava, Tinosporae Radix, and Catharanthus Roseus. Regulation No. 7 of 2018 (BPOM, 2018) also prohibits the presence of berberine in processed food. This research was conducted to determine the content of berberine compounds from the extract and stem fraction of Arcangelisia flava. The research was conducted by identifying the content of berberine by TLC method compared with Rf Berberine sulfate and determination of berberine content by HPLC method (High-Performance Liquid Chromatography) using column C-18 (ODS). Berberine content of methanol extract, n-hexane fraction, ethyl acetate fraction and Arcangelisia Flava methanol-water fraction were 0.0040, respectively; 0.0010; 0.0041, 0.0044%

The orchids genus Dilochia in Indonesia

Five species of Dilochia (D. cantleyi, D. longilabris, D. parviflora, D. rigida and D. wallichii) have been recognized in Indonesia. One species from Sumatra is proposed as new species. Descriptions and identification key to all species based on morphological characters are presented

THE ORCHID GENUS LUISIA IN INDONESIA

Nine species and one new variety of the genus Luisia are recognized in Indonesia. The newly proposed variety is L. zollingeri Rchb. f. var. iati-petala ( J. J. S. ) Sulistiarini. Complete descriptions, key to all species based on morphological characters as well as lists of specimens examined are presented

KEBUDAYAAN INDIS SEBAGAI HASIL PENGARUH KEBUDAYAAN BARAT DI INDONESIA

Kebudayaan Indis lahir sebagai hasil akulturasi kebudayaan Barat dan kebudayaan pribumi. Kedatangan Belanda ke Indonesia diikuti dengan dibawanya kebudayaan Belanda yang kemudian mengalami percampuran dengan kebudayaan lokal melalui berbagai jalur. Kebudayaan Indis sebagai hasil pengaruh kebudayaan Barat di Indonesia pun menarik untuk dibahas untuk mengetahui apa saja bentuk kebudayaan Indis yang ada. Metode penelitian yang digunakan adalah metode historiografi sejarah yang terdiri dari tahap pengumpulan data, verifikasi, interpretasi, dan historiografi. Dari hasil penelitian, berkembangnya kebudayaan Indis juga dipengaruhi politik etis dalam edukasi. Berbagai kebudayaan Indis yang ada di antaranya adalah gaya hidup yang meliputi gaya berbusana, kebiasaan-kebiasaan, dan bahasa. Kemudian rijsttafel sebagai bentuk jamuan makan ala Belanda, lalu arsitektur bangunan yang juga tak luput dari pengaruh kebudayaan Indis, baik bangunan tempat tinggal, stasiun, gereja, dan lain-lain

EFFECTIVENESS OF WASTE MANAGEMENT AS A SOURCE OF ELECTRICAL ENERGY IN TPA BENOWO, SURABAYA CITY

Waste management is very important to achieve a clean and healthy environmental quality, thus waste must be managed as well as possible by implementing established planning and regulations so that things that are negative for life do not happen. Reuse accompanied by reduced usage is an effective step to overcome existing problems and the lifestyle of the community must be changed with existing waste to be processed into something of value, therefore the government has created innovations in overcoming waste problems, namely using environmentally friendly technology or PLTSa Garbage power plant). The purpose of this research is to describe, know and analyze the effectiveness of waste management as a source of electrical energy in TPA Benowo, Surabaya. This research is a form of research conducted with a qualitative approach. Data collection techniques in this study are interviews, observation and documentation to obtain information related to environmentally friendly technological innovations. The results show that the effectiveness of waste management as a source of electrical energy in TPA Benowo, Surabaya city is effective and is running according to existing regulations by measuring indicators from Riant Nugroho's theory so that with Appropriate the effectiveness of established policies can turn waste into useful goods. with environmentally friendly technology

Pemanfaatan Citra Alos Avnir-2 Dan Sistem Informasi Geografis Untuk Menentukan Tingkat Kerawanan Wilayah Terhadap Malaria Secara Spasio Temporal Di Kabupaten Purworejo

Tujuan penelitian ini adalah (1) Mengetahui parameter lingkungan yang mempengaruhi perkembangbiakan vektor malaria; (2) Mengetahui kemampuan citra ALOS AVNIR-2 dalam menyajikan parameter lingkungan; (3) Menentukan tingkat kerawanan wilayah terhadap malaria secara spasio temporal; dan (4) Mengetahui validasi antara Peta Kerawanan Wilayah Terhadap Malaria dengan Peta MOPI (Month Parasite Incidence). Parameter penggunaan lahan, kerapatan vegetasi, curah hujan, ketinggian tempat, dan tekstur tanah berpengaruh untuk perkembangbiakan vektor malaria, sedangkan suhu udara pengaruhnya lemah. Citra AVNIR-2 dapat menyajikan informasi kerapatan vegetasi dan penggunaan lahan dengan ketelitian interpretasi 97,17%. Peta Tingkat Kerawanan Terhadap Malaria diklasifikasikan menjadi tujuh, yaitu sangat rawan, rawan, cukup rawan, sedikit rawan, cukup tidak rawan, tidak rawan, dan sangat tidak rawan. Peta Tingkat Kerawanan Terhadap Malaria di Kabupaten Purworejo menunjukkan tingkat kerawanan yang sesuai dan tidak sesuai dengan Peta MOPI karena adanya faktor-faktor sosial yang berpengaruh di dalamnya