Leaching of nickel laterite using fungus mediated organic acid and synthetic organic acid: a comparative study

A huge amount of overburden (nearly 8 to 10 times of the ore) containing trace amount of nickel and cobalt is generated during Chromite mining at Sukinda valley, Orissa, Chromite overburden contains around 0.4 to 0.9% Ni and 0.02–0.05% Co respectively. The setting up of nickel and cobalt processing plant based on these deposits through conventional methods such as pyrometallurgy and hydrometallurgy is not economical. The microbes and metals interaction have been studied for the exploitation in metals extraction. So an attempt has been made to extract these metals using multi metal resistant indigenous microorganisms, isolated from the Chromite overburden of Sukinda mines. A native strain of Aspergillus species was used for bioleaching. Aspergillus species are well known for their potential to produce a variety of organic acids (oxalic, citric acids etc.). The mineralogical studies indicated that there is no separate nickel bearing mineral phase in the Sukinda Chromite overburden. The mineralogy of the raw lateritic ore reveals the presence of goethite, ferrihydrites as major minerals. In the thermally activated overburden the minerals present were hematite, surimarite, quartz and traces of magnetite. Experiments were carried out with synthetic organic acids at 2.5% pulp density, 350C and 150rpm. Synthetic oxalic acid (0.1 M) leached 5% Ni and 71% Co from raw ore, whereas it leached 43% Ni and 95% Co from thermally activated ore. Citric acid (0.1 M) was not that much efficient. It leached 9% Ni and 14% Co from raw ore and 32% Ni and 45% Co from thermally activated ore. The fungal culture filtrate leached 3% Ni and 12% Co from raw ore. In case of roasted ore it leached 18% Ni and 28% Co at 2.5% pulp density, 35°C and 150rpm. Mineralogical analysis was carried out through X-ray diffraction, FTIR and transmission electron microscopy

FACt: FORTRAN toolbox for calculating fluctuations in atomic condensates

We develop a FORTRAN code to compute fluctuations in atomic condensates (FACt) by solving the Bogoliubov-de Gennes (BdG) equations for two component Bose-Einstein condensate (TBEC) in quasi two dimensions. The BdG equations are recast as matrix equations and solved self consistently. The code is suitable for handling quantum fluctuations as well as thermal fluctuations at temperatures below the critical point of Bose-Einstein condensation. The code is versatile, and the ground state density profile and low energy excitation modes obtained from the code can be easily adapted to compute different properties of TBECs -- ground state energy, overlap integral, quasi particle amplitudes of BdG spectrum, dispersion relation and structure factor and other related experimental observables.Comment: The manuscript contains 28 pages and 6 figures. The code including sample input file and README file can be found in a tar file 'hfb2d2s.tar' by clicking on the "other formats" in the paper download pag

Spectral analysis for compressible quantum fluids

Turbulent fluid dynamics typically involves excitations on many different length scales. Classical incompressible fluids can be cleanly represented in Fourier space enabling spectral analysis of energy cascades and other turbulence phenomena. In quantum fluids, additional phase information and singular behaviour near vortex cores thwarts the direct extension of standard spectral techniques. We develop a formal and numerical spectral analysis for $U(1)$ symmetry-breaking quantum fluids suitable for analyzing turbulent flows, with specific application to the Gross-Pitaevskii fluid. Our analysis builds naturally on the canonical approach to spectral analysis of velocity fields in compressible quantum fluids, and establishes a clear correspondence between energy spectral densities, power spectral densities, and autocorrelation functions, applicable to energy residing in velocity, quantum pressure, interaction, and potential energy of the fluid. Our formulation includes all quantum phase information and also enables arbitrary resolution spectral analysis, a valuable feature for numerical analysis. A central vortex in a trapped planar Bose-Einstein condensate provides an analytically tractable example with spectral features of interest in both the infrared and ultraviolet regimes. Sampled distributions modelling the dipole gas, plasma, and clustered regimes exhibit velocity correlation length increasing with vortex energy, consistent with known qualitative behaviour across the vortex clustering transition. The spectral analysis of compressible quantum fluids presented here offers a rigorous tool for analysing quantum features of superfluid turbulence in atomic or polariton condensates.Comment: 17 pages. Fixed error in appendix C presentation, added references. Results and conclusions unchange

Dissolution of heavy metals from electrostatic precipitator (ESP) dust of a coal based sponge iron plant by fungal leaching

Coal based sponge iron industries in India generate considerable quantity of solid waste, 40% of which is flue dust produced from the electrostatic precipitator (ESP) connected to rotary kiln. This paper reports the dissolution of Zn, Cu, Pb, Mn and Fe from the ESP dust using three fungal species, Aspergillus niger, Aspergillus fumigatus and Aspergillus flavus at 5 and 10% pulp densities over a period of 28 days. Highest metal leaching was achieved with A. niger followed by A. flavus. The least metal leaching was achieved with A. fumigatus. The pH of the medium declined consistently over the incubation period. Maximum leaching for Zn, Cu, Pb, Mn and Fe were 81, 76, 74, 72 and 52% respectively.Key words: Fungal leaching, sponge iron, electrostatic precipitator (ESP) dust, metal dissolution

Immune Responses to Defined Plasmodium Falciparum Antigens and Disease Susceptibility in Two Subpopulations of Northern India

The aim of this study was to investigate the prevalence of naturally acquired immune response to malaria in individuals of different age groups belonging to areas of northern India, Loni PHC (LN) and Dhaulana PHC (SD) of district Ghaziabad. Plasmodium falciparum-infected erythrocyte lysate and six synthetic peptides from different stages of P. falciparum (CSP, MSP1, AMA1, RAP1, EBA175 and PfG27) were used to determine both humoral and cellular immune responses. Plasma of individual subject was also analyzed for IL-4, IL-10, IFN-γ and TNF-α level. We observed an age-wise increasing trend of immunity in these two populations. There was a significant association between the number of antibody responders and recognition of stage-specific epitopes by antibodies. Peripheral blood mononuclear cells of more than 75% of individuals proliferated in response to stimulation by all the antigens in LN area. IL-4 and IL-10 responses were significantly higher in individuals of LN Area; whereas IFN-g and   TNF-a responses were higher in individuals of SD Area. It was also noticed that the frequency of responders to stage-specific antigens was higher in individuals from the LN area where the frequency of malaria was lower. The naturally acquired immune responses to P. falciparum antigens reflected the reduced risk of malaria in the study groups. The results demonstrated immunogenicity of the epitopes to P. falciparum in population of this endemic zone

Isolation and Purification of C-phycocyanin From Nostoc Muscorum (Cyanophyceae and Cyanobacteria) Exhibits Antimalarial Activity in Vitro

The phycobilin pigments are intensively fluorescent and water soluble. They are categorized into three types, such as pigments containing high, intermediate and low energies are phycoerythrins (phycoerythrocyanins), phycocyanins and allophycocyanins, respectively. Besides light harvesting, the phycobiliproteins have shown industrial and biomedical importance. Among them, C-phycocyanin (C-PC) has been considered to be the most preferred one. The present study was undertaken to evaluate the antimalarial activity of C-PC isolated from a nitrogen-fixing cyanobacterium and Nostoc muscorum. C-PC was extracted and purified by acetone extraction and ammonium sulfate precipitation and dialysis followed by amicon filtration. It was isolated as a~124 kDa water soluble protein molecule. It showed antimalarial activity in vitro against chloroquine sensitive and resistant Plasmodium falciparum strains. Inhibitory concentrations at 50%, 90% and 95% were determined as 10.27±2.79, 53.53±6.26 and 73.78±6.92 µg/ml against the chloroquine-sensitive strains; 10.37±1.43, 56.99±11.07 and 72.79±8.59 µg/ml against chloroquine resistant of Plasmodium falciparum strains. C-PC was found to have antimalarial activity even at a concentration of 3.0µg/ml. The possible mechanism might be relied on the destruction of polymerization of haemozoin by binding of C-PC with ferriprotoporphyrin-IX at the water surface of the plasma membrane

Isolation and Purification of C-phycocyanin From Nostoc Muscorum (Cyanophyceae and Cyanobacteria) Exhibits Antimalarial Activity in Vitro

The phycobilin pigments are intensively fluorescent and water soluble. They are categorized into three types, such as pigments containing high, intermediate and low energies are phycoerythrins (phycoerythrocyanins), phycocyanins and allophycocyanins, respectively. Besides light harvesting, the phycobiliproteins have shown industrial and biomedical importance. Among them, C-phycocyanin (C-PC) has been considered to be the most preferred one. The present study was undertaken to evaluate the antimalarial activity of C-PC isolated from a nitrogen-fixing cyanobacterium and Nostoc muscorum. C-PC was extracted and purified by acetone extraction and ammonium sulfate precipitation and dialysis followed by amicon filtration. It was isolated as a~124 kDa water soluble protein molecule. It showed antimalarial activity in vitro against chloroquine sensitive and resistant Plasmodium falciparum strains. Inhibitory concentrations at 50%, 90% and 95% were determined as 10.27±2.79, 53.53±6.26 and 73.78±6.92 µg/ml against the chloroquine-sensitive strains; 10.37±1.43, 56.99±11.07 and 72.79±8.59 µg/ml against chloroquine resistant of Plasmodium falciparum strains. C-PC was found to have antimalarial activity even at a concentration of 3.0µg/ml. The possible mechanism might be relied on the destruction of polymerization of haemozoin by binding of C-PC with ferriprotoporphyrin-IX at the water surface of the plasma membrane

Statistical optimization for lipase production from solid waste of vegetable oil industry

<p>The production of biofuel using thermostable bacterial lipase from hot spring bacteria out of low-cost agricultural residue olive oil cake is reported in the present paper. Using a lipase enzyme from <i>Bacillus licheniformis</i>, a 66.5% yield of methyl esters was obtained. Optimum parameters were determined, with maximum production of lipase at a pH of 8.2, temperature 50.8°C, moisture content of 55.7%, and biosurfactant content of 1.693 mg. The contour plots and 3D surface responses depict the significant interaction of pH and moisture content with biosurfactant during lipase production. Chromatographic analysis of the lipase transesterification product was methyl esters, from kitchen waste oil under optimized conditions, generated methyl palmitate, methyl stearate, methyl oleate, and methyl linoleate.</p

Genetic structure of Plasmodium falciparum field isolates in eastern and north-eastern India

<p>Abstract</p> <p>Background</p> <p>Molecular techniques have facilitated the studies on genetic diversity of <it>Plasmodium </it>species particularly from field isolates collected directly from patients. The <it>msp-1 </it>and <it>msp-2 </it>are highly polymorphic markers and the large allelic polymorphism has been reported in the block 2 of the <it>msp-1 </it>gene and the central repetitive domain (block3) of the <it>msp-2 </it>gene. Families differing in nucleotide sequences and in number of repetitive sequences (length variation) were used for genotyping purposes. As limited reports are available on the genetic diversity existing among <it>Plasmodium falciparum </it>population of India, this report evaluates the extent of genetic diversity in the field isolates of <it>P. falciparum </it>in eastern and north-eastern regions of India.</p> <p>Methods</p> <p>A study was designed to assess the diversity of <it>msp-1 </it>and <it>msp-2 </it>among the field isolates from India using allele specific nested PCR assays and sequence analysis. Field isolates were collected from five sites distributed in three states namely, Assam, West Bengal and Orissa.</p> <p>Results</p> <p><it>P. falciparum </it>isolates of the study sites are highly diverse in respect of length as well as sequence motifs with prevalence of all the reported allelic families of <it>msp-1 </it>and <it>msp-2</it>. Prevalence of identical allelic composition as well as high level of sequence identity of alleles suggest a considerable amount of gene flow between the <it>P. falciparum </it>populations of different states. A comparatively higher proportion of multiclonal isolates as well as multiplicity of infection (MOI) was observed among isolates of highly malarious districts Karbi Anglong (Assam) and Sundergarh (Orissa). In all the five sites, R033 family of <it>msp-1 </it>was observed to be monomorphic with an allele size of 150/160 bp. The observed 80–90% sequence identity of Indian isolates with data of other regions suggests that Indian <it>P. falciparum </it>population is a mixture of different strains.</p> <p>Conclusion</p> <p>The present study shows that the field isolates of eastern and north-eastern regions of India are highly diverse in respect of <it>msp-1 </it>(block 2) and <it>msp-2 </it>(central repeat region, block 3). As expected Indian isolates present a picture of diversity closer to southeast Asia, Papua New Guinea and Latin American countries, regions with low to meso-endemicity of malaria in comparison to African regions of hyper- to holo-endemicity.</p

Nerve growth factor induces neurite outgrowth of PC12 cells by promoting Gβγ-microtubule interaction

Background: Assembly and disassembly of microtubules (MTs) is critical for neurite outgrowth and differentiation. Evidence suggests that nerve growth factor (NGF) induces neurite outgrowth from PC12 cells by activating the receptor tyrosine kinase, TrkA. G protein-coupled receptors (GPCRs) as well as heterotrimeric G proteins are also involved in regulating neurite outgrowth. However, the possible connection between these pathways and how they might ultimately converge to regulate the assembly and organization of MTs during neurite outgrowth is not well understood. Results: Here, we report that Gβγ, an important component of the GPCR pathway, is critical for NGF-induced neuronal differentiation of PC12 cells. We have found that NGF promoted the interaction of Gβγ with MTs and stimulated MT assembly. While Gβγ-sequestering peptide GRK2i inhibited neurite formation, disrupted MTs, and induced neurite damage, the Gβγ activator mSIRK stimulated neurite outgrowth, which indicates the involvement of Gβγ in this process. Because we have shown earlier that prenylation and subsequent methylation/demethylation of γ subunits are required for the Gβγ-MTs interaction in vitro, small-molecule inhibitors (L-28 and L-23) targeting prenylated methylated protein methyl esterase (PMPMEase) were tested in the current study. We found that these inhibitors disrupted Gβγ and ΜΤ organization and affected cellular morphology and neurite outgrowth. In further support of a role of Gβγ-MT interaction in neuronal differentiation, it was observed that overexpression of Gβγ in PC12 cells induced neurite outgrowth in the absence of added NGF. Moreover, overexpressed Gβγ exhibited a pattern of association with MTs similar to that observed in NGF-differentiated cells. Conclusions: Altogether, our results demonstrate that βγ subunit of heterotrimeric G proteins play a critical role in neurite outgrowth and differentiation by interacting with MTs and modulating MT rearrangement. Electronic supplementary material The online version of this article (doi:10.1186/s12868-014-0132-4) contains supplementary material, which is available to authorized users