Using Ethnotaxonomy to assess Traditional Knowledge and Language vitality: A case study with the Urang Kanekes (Baduy) of Banten, Indonesia

576-582The Urang Kanekes of Banten, Indonesia are culturally divided into two groups, namely Urang Jero (inner Kanekes) who follow the traditions and customs strictly, and an Urang Luar (outer Kanekes) who tolerate minor deviations from the customs and traditions. The present study looks into the vitality status of the traditional knowledge (TK) and language of the community, comparing trends from the inner and outer Kanekes. Sixteen knowledgeable elders from the Urang Jero (inner Kanekes) were interviewed in the first phase, while thirty randomly chosen adult members from the Urang Luar (outer Kanekes) and another 30 from the Urang Jero (inner Kanekes) were interviewed in the second phase. Interviews followed the methodology stipulated by the TK and Language Vitality Index (TraLaVi) which was used to analyse the vitality of TK and language. The results of the study show that the Urang Kanekes have been successful in maintaining high TK and language vitality, and there is no significant difference in the vitality status of TK and language between the inner and outer Kanekes people

eSnail: a transcriptome-based molecular resource of the central nervous system for terrestrial gastropods

In the zip file, we provided assembled contig files, protein sequences, annotation and single nucleotide polymorphism for six terrestrial gastropods: Cochlicella acuta, Helix aspersa, Deroceras invadens, Deroceras reticulatum, Lehmannia nyctelia, and Milax gagates

Data from: eSnail: a transcriptome-based molecular resource of the central nervous system for terrestrial gastropods

To expand on emerging terrestrial gastropod molecular resources, we have undertaken transcriptome-based sequencing of the central nervous system (CNS) from six ecologically invasive terrestrial gastropods. Focusing on snail species Cochlicella acuta and Helix aspersa, and reticulated slugs Deroceras invadens, Deroceras reticulatum, Lehmannia nyctelia, and Milax gagates, we obtained a total of 367,869,636 high quality reads and compared them with existing CNS transcript resources for the invasive Mediterranean snail, Theba pisana. In total we obtained, 419,289 unique transcripts (unigenes) from 1,410,569 assembled contigs, with BLAST search analysis of multiple protein databases leading to the annotation of 124,268 unigenes, of which 92,544 mapped to NCBI non-redundant protein databases. We found that these transcriptomes have representatives in most biological functions, based on comparison of gene ontology, KEGG pathway, and protein family contents, demonstrating a high range of transcripts responsible for regulating metabolic activities and molecular functions occurring within the CNS. To provide an accessible genetic resource, we also demonstrate the presence of 66,687 microsatellites and 304,693 single nucleotide variants, which can be used for the design of potentially thousands of unique primers for functional screening. An online ‘eSnail’ database with a user-friendly web interface was implemented to query all the information obtained herein (http://soft.bioinfo-minzhao.org/esnail). We demonstrate the usefulness of the database through the mining of molluscan neuropeptides. As the most comprehensive CNS transcriptome resource for terrestrial gastropods, eSnail may serve as a useful gateway for researchers to explore gastropod CNS function for multiple purposes, including for the development of biocontrol approaches