When 'farewell' is not 'goodbye' : a rhetorical reading of the farewell discourse : John 13-17

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Occupational Therapy Treatments and Upper Extremity Motor and Self-Care Outcomes of Patients Post-CVA

Cerebrovascular accident (or stroke) afflicts approximately 550,000 Americans per year. It is estimated that three million U.S. citizens live with the disabling effects of strokes, which can limit function in one or more of the following areas: mobility, basic activities of daily living, bowel or bladder control, cognition, emotional functioning, among other disabling conditions (Gresham et al., 1995). Occupational therapists, as rehabilitation professionals, provide sensorimotor and basic activities of daily living assessment and intervention to promote recovery of function post-stroke. Upper extremity neuromuscular electrical stimulation is one strategy that may be used to promote motor recovery. This study\u27s purpose was to investigate the type of post-stroke variables (i.e., demographic, selected medical, and rehabilitative treatments) which contributed to and predicted improved upper extremity motor and activity of daily living outcomes within the context of an acute rehabilitation inpatient hospital setting. One specific treatment, neuromuscular electrical stimulation, was studied for its impact upon overall upper extremity motor and daily living outcomes. Medical records (N = 136) served as the primary data source for this study\u27s retrospective document review. Electrical stimulation was provided as a treatment to 13.2% of the sample. Overall, this study found that in the comparison of the subgroups receiving electrical stimulation or not, the only significant difference was in muscle tone or spasticity. When the two subgroups were compared by type of CVA or admit to discharge change scores, additional significant differences were observed on some daily living and motor variables. Specifically, the left brain etiology yielded more findings of significant difference than the right brain etiology. Other study findings included significant differences in admit to discharge ratings of motor, self-care, and functional ratings for the entire sample and significant positive relationships between right or left hand strength and self-care ability. Higher self-care was predictive of higher cognitive ratings; and two OT function tests were predictive of higher self-care skill in a regression analysis

Interactions between Bromus tectorum L. (cheatgrass) and native ruderal species in ecological restoration

2012 Fall.Includes bibliographical references.Bromus tectorum L. is an invasive annual grass that dominates much of the rangeland in western North America. It has shown an impressive ability to invade ecosystems, causing substantial changes in the composition and function of native plant and soil communities in ways that promote further exotic invasion through displacement of native plant species and slowing or halting of succession. B. tectorum has several characteristics, including high reproductive rates, affinity for disturbed sites, and the ability to create positive feedback conditions in invaded sites, which make it highly successful at invading new sites, and extremely challenging to remove during revegetation efforts. Traditional control methods including herbicide application, grazing, and burning have largely proven unsuccessful at preventing establishment and spread of B. tectorum. Similarly, restoration seed mixes often consist of native perennial grass species, which tend to be slower growing and less robust in disturbed sites, and therefore provide little competition against B. tectorum and do not promote the reestablishment of native plant communities. In addition, seed mixes are often planted at a fraction of the rate of annual B. tectorum seed production, giving them a distinct disadvantage. Native ruderal species share many traits with B. tectorum and could potentially compete with this invader if used at high, competitive seeding rates in restoration efforts, and may alter site characteristics in ways that promote succession of the native plant community. One key characteristic that may be closely associated with community development is the development and composition of the arbuscular mycorrhizal fungi (AMF) community. AMF are important for resource acquisition by a majority of plant species. They are particularly important for late-seral plant species, which typically exist in low available nutrient conditions. Bromus tectorum causes shifts in the mycorrhizal community that could lead to a loss of AMF species richness and abundance in a very short time period, resulting in conditions that are difficult for late-seral species to colonize, due to a lack of access to resources through host-specific plant-AMF relationships. Utilizing native seed mixes composed of species selected for specific functional and competitive traits, and mycorrhizal status, and creating seeding rates designed to increase interspecific competition with B. tectorum may provide the missing link for successful restoration of B. tectorum-invaded sites. A study was conducted in northern Colorado to determine whether native ruderal species could suppress B. tectorum establishment and persistence in a disturbed site, and how these effects compared to similar effects by sterile wheat. In this study, B. tectorum was seeded with and without a high rate native ruderal seed mix and a sterile wheat species (QuickGuardTM) used in revegetation efforts for erosion control. Bromus tectorum biomass and density data were collected, as well as biomass and density for all seeded native species and sterile wheat. All treatments were seeded in the fall of 2010, and vegetative data collection occurred during the summers of 2011 and 2012. To assess the immediate effects of the establishing plant community on the AMF community, soils were collected from three of the field study treatments after one year of growth: 1) B. tectorum, 2) a mixture of native early-seral species, or 3) B. tectorum plus native early-seral species. Three mycorrhizal host plant species (Bouteloua gracilis (Willd. ex Kunth) Lag. ex Griffiths, Ratibida columnifera (Nutt.) Woot. & Standl., Sorghum bicolor (L.) Moench ssp. drummondii (Nees ex Steud.) de Wet & Harlan) were grown in these soils under greenhouse conditions. Roots were harvested after 30 days and analyzed for AMF colonization. After one growing season, the native ruderal mix significantly reduced B. tectorum biomass in the field. After the second growing season, the effect was no longer detectable in biomass measurements, but was still observed as a reduction in density of B. tectorum in the native ruderal plots. The sterile wheat reduced B. tectorum biomass after one growing season, but to a lesser extent than the native mix, and had a positive effect on density. In 2012, effects on both biomass and density disappeared. Results from the AMF colonization analysis indicated that the presence of the native species had an impact on AMF richness or abundance within the soils and that B. tectorum alters the AMF community in a way that is unique in comparison to weedy native vegetation. Soils from beneath native early-seral plant species had much higher colonization of the host plant species relative to soils from beneath B. tectorum. In addition, the native host R. columnifera had much higher rates of colonization than the non-native host, S. bicolor, indicating that there may be some host-dependent plant-AMF relationships that are more beneficial to the native plant than the non-native plant. The results of these studies could have important practical field applications for restoring invaded sites, particularly when the goal is to create conditions that promote development of late-seral native plant communities. Utilization of native ruderal species in revegetation mixes could provide a critical missing link for facilitation of late-seral, native plant communities through suppression of B. tectorum, as well as rapid facilitation of AMF communities that successfully colonize native late-seral host species. Continued monitoring and assessment of this study site could lend further insight to the long-term dynamics of the native ruderal plant community with B. tectorum and development of a late-seral plant community

Biblioteket skal integreres i studiemiljøerne

’Book en bibliotekar’ er en populær ordning på Roskilde Universitetsbibliotek. Men der er i det hele taget behov for, at biblioteket i højere grad opsøger de studerende, hvis biblioteket skal blive en del af de studerendes informationslandskab

A Novel Prokaryote-Type ECF/ABC Transporter Module in Chloroplast Metal Homeostasis

During evolution, chloroplasts, which originated by endosymbiosis of a prokaryotic ancestor of today's cyanobacteria with a eukaryotic host cell, were established as the site for photosynthesis. Therefore, chloroplast organelles are loaded with transition metals including iron, copper, and manganese, which are essential for photosynthetic electron transport due to their redox capacity. Although transport, storage, and cofactor-assembly of metal ions in chloroplasts are tightly controlled and crucial throughout plant growth and development, knowledge on the molecular nature of chloroplast metal-transport proteins is still fragmentary. Here, we characterized the soluble, ATP-binding ABC-transporter subunits ABCI10 and ABCI11 in Arabidopsis thaliana, which show similarities to components of prokaryotic, multisubunit ABC transporters. Both ABCI10 and ABCI11 proteins appear to be strongly attached to chloroplast-intrinsic membranes, most likely inner envelopes for ABCI10 and possibly plastoglobuli for ABCI11. Loss of ABCI10 and ABCI11 gene products in Arabidopsis leads to extremely dwarfed, albino plants showing impaired chloroplast biogenesis and deregulated metal homeostasis. Further, we identified the membrane-intrinsic protein ABCI12 as potential interaction partner for ABCI10 in the inner envelope. Our results suggest that ABCI12 inserts into the chloroplast inner envelope membrane most likely with five predicted a-helical transmembrane domains and represents the membrane-intrinsic subunit of a prokaryotic-type, energy-coupling factor (ECF) ABC-transporter complex. In bacteria, these multisubunit ECF importers are widely distributed for the uptake of nickel and cobalt metal ions as well as for import of vitamins and several other metabolites. Therefore, we propose that ABCI10 (as the ATPase A-subunit) and ABCI12 (as the membrane-intrinsic, energy-coupling T-subunit) are part of a novel, chloroplast envelope-localized, AAT energy-coupling module of a prokaryotic-type ECF transporter, most likely involved in metal ion uptake.11Nsciescopu

New Development in NASA's Rodent Research Hardware for Conducting Long Duration Biomedical and Basic Research in Space

Animal models, particularly rodents, are the foundation of pre-clinical research to understand human diseases and evaluate new therapeutics, and play a key role in advancing biomedical discoveries both on Earth and in space. The National Research Councils Decadal survey emphasized the importance of expanding NASAs life sciences research to perform long duration, rodent experiments on the International Space Station (ISS). To accomplish this objective, flight hardware, operations, and science capabilities were developed at NASA Ames Research Center (ARC) to enhance science return for both commercial (CASIS) and government-sponsored rodent research. The Rodent Research program at NASA ARC has pioneered a new research capability on the International Space Station and has progressed toward translating research to the ISS utilizing commercial rockets, collaborating with academia and science industry, while training crewmembers to assist in performing research on orbit. Throughout phases of these missions, our practices, hardware and operations have evolved from tested to developed standards, and we are able to modify and customize our procedure and operations for mission specific requirements. The Rodent Research Habitat is capable of providing a living environment for animals on ISS according to standard animal welfare requirements. Using the cameras in the Habitat, the Rodent Research team has the ability to perform daily health checks on animals, and further analyze the collected videos for behavioral studies. A recent development of the Rodent Research hardware is inclusion of enrichment, to provide the animals the ability to rest and huddle. The Enrichment Hut is designed carefully for adult mice (up to 35 week old) within animal welfare, engineering, and operations constraints. The Hut is made out of the same stainless steel mesh as the cage interior, it has an ingress and an egress to allow animals move freely, and a hinge door to allow crewmembers remove the animals easily. The Rodent Research team has also developed Live Animal Return (LAR) capability, which will be implemented during Rodent Research-5 mission for the first time. The animals will be transported from the Habitat to a Transporter, which will return on the Dragon capsule and splashes down in the Pacific Ocean. Once SpaceX retrieves the Dragon, all powered payloads will be transferred to a SeaVan and transferred to the Long Beach pier. The NASA team then receives the transporter and delivers to a PI-designated laboratory within 120 mile radius of Long Beach. This is a significant improvement allowing researchers to examine animals within 72 hrs. of reentry or to conduct recovery experiments. Together, the hardware improvements and experience that the Rodent Research team has gained working with principal investigators and ISS crew to conduct complex experiments on orbit are expanding capabilities for long duration rodent research on the ISS to achieve both basic science and biomedical objectives

ESTRATÉGIAS DE COPING UTILIZADAS POR TRABALHADORES DE ENFERMAGEM EM TERAPIA INTENSIVA NEONATAL

RESUMO Estudo quantitativo, transversal e descritivo desenvolvido com 23 trabalhadores de enfermagem com o objetivo de identificar as estratégias de coping utilizadas por trabalhadores de enfermagem de uma unidade de terapia intensiva neonatal. Os dados foram coletados em setembro e outubro de 2012 por meio de formulário para caracterização sociodemográfica/funcional e Inventário de Estratégias de Coping. Projeto aprovado por Comitê de Ética em Pesquisa, CAAE 06163312.8.0000.5346. Após análise dos resultados verificou-se que os fatores do inventário mais utilizados pelos trabalhadores foram autocontrole, reavaliação positiva e suporte social. Essas estratégias, centradas tanto na emoção quanto no problema, são consideradas ativas, pois conduzem para a tomada de decisão na resolução da situação estressora. Concluiu-se que identificar as estratégias de coping utilizadas pelos trabalhadores de enfermagem de terapia intensiva pode possibilitar o conhecimento de como as situações estressoras são enfrentadas e favorecer o planejamento de ações de educação permanente, para sensibilizar e instrumentalizar os trabalhadores para o uso efetivo do coping