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Maintenance and degradation of proteins in intact and severed axons: Implications for the mechanism of long-term survival of anucleate crayfish axons
Protein maintenance and degradation are examined in the
severed distal (anucleate) portions of crayfish medial giant
axons (MGAs), which remain viable for over 7 months following
axotomy. On polyacrylamide gels, the silver-stained
protein banding pattern of anucleate MGAs severed from
their cell bodies for up to 4 months remains remarkably similar
to that of intact MGAs. At 7 months postseverance, some
(but not all) proteins are decreased in anucleate MGAs compared
to intact MGAs. To determine the half-life of axonally
transported proteins, we radiolabeled MGA cell bodies and
monitored the degradation of newly synthesized transported
proteins. Assuming exponential decay, proteins in the fast
component of axonal transport have an average half-life of
14 d in anucleate MGAs and proteins in the slow component
have an average half-life of 17 d. Such half-lives are very
unlikely to account for the ability of anucleate MGAs to survive
for over 7 months after axotomy.This work was supported by an ATP grant to G.D.B.Neuroscienc
Absolute Determination of the 22Na(p,g) Reaction Rate in Novae
Gamma-ray telescopes in orbit around the Earth are searching for evidence of
the elusive radionuclide 22Na produced in novae. Previously published
uncertainties in the dominant destructive reaction, 22Na(p,g)23Mg, indicated
new measurements in the proton energy range of 150 to 300 keV were needed to
constrain predictions. We have measured the resonance strengths, energies, and
branches directly and absolutely by using protons from the University of
Washington accelerator with a specially designed beamline, which included beam
rastering and cold vacuum protection of the 22Na implanted targets. The
targets, fabricated at TRIUMF-ISAC, displayed minimal degradation over a ~ 20 C
bombardment as a result of protective layers. We avoided the need to know the
stopping power, and hence the target composition, by extracting resonance
strengths from excitation functions integrated over proton energy. Our
measurements revealed that resonance strengths for E_p = 213, 288, 454, and 610
keV are stronger by factors of 2.4 to 3.2 than previously reported. Upper
limits have been placed on proposed resonances at 198-, 209-, and 232-keV. We
have re-evaluated the 22Na(p,g) reaction rate, and our measurements indicate
the resonance at 213 keV makes the most significant contribution to 22Na
destruction in novae. Hydrodynamic simulations including our rate indicate that
the expected abundance of 22Na ejecta from a classical nova is reduced by
factors between 1.5 and 2, depending on the mass of the white-dwarf star
hosting the nova explosion.Comment: 20 pages, 18 figures; shortened paper, accepted in Phys. Rev.
InAs nanowire transistors with multiple, independent wrap-gate segments
We report a method for making horizontal wrap-gate nanowire transistors with
up to four independently controllable wrap-gated segments. While the step up to
two independent wrap-gates requires a major change in fabrication methodology,
a key advantage to this new approach, and the horizontal orientation more
generally, is that achieving more than two wrap-gate segments then requires no
extra fabrication steps. This is in contrast to the vertical orientation, where
a significant subset of the fabrication steps needs to be repeated for each
additional gate. We show that cross-talk between adjacent wrap-gate segments is
negligible despite separations less than 200 nm. We also demonstrate the
ability to make multiple wrap-gate transistors on a single nanowire using the
exact same process. The excellent scalability potential of horizontal wrap-gate
nanowire transistors makes them highly favourable for the development of
advanced nanowire devices and possible integration with vertical wrap-gate
nanowire transistors in 3D nanowire network architectures.Comment: 18 pages, 5 figures, In press for Nano Letters (DOI below
Cepheid variables in the LMC cluster NGC 1866. I. New BVRI CCD photometry
We report BV(RI)c CCD photometric data for a group of seven Cepheid variables
in the young, rich cluster NGC 1866 in the Large Magellanic Cloud. The
photometry was obtained as part of a program to determine accurate distances to
these Cepheids by means of the infrared surface brightness technique, and to
improve the LMC Cepheid database for constructing Cepheid PL and PLC relations.
Using the new data together with data from the literature, we have determined
improved periods for all variables. For five fundamental mode pulsators, the
light curves are now of excellent quality and will lead to accurate distance
and radius determinations once complete infrared light curves and radial
velocity curves for these variables become available.Comment: To appear in ApJ Supp., AASTeX, 24 pages, 8 tables, 8 figure
Peer Support Specialists and Service Users’ Perspectives on privacy, confidentiality, and security of digital mental health
As the digitalization of mental health systems progresses, the ethical and social debate on the use of these mental health technologies has seldom been explored among end-users. This article explores how service users (e.g., patients and users of mental health services) and peer support specialists understand and perceive issues of privacy, confidentiality, and security of digital mental health interventions. Semi-structured qualitative interviews were conducted among service users (n = 17) and peer support specialists (n = 15) from a convenience sample at an urban community mental health center in the United States. We identified technology ownership and use, lack of technology literacy including limited understanding of privacy, confidentiality, and security as the main barriers to engagement among service users. Peers demonstrated a high level of technology engagement, literacy of digital mental health tools, and a more comprehensive awareness of digital mental health ethics. We recommend peer support specialists as a potential resource to facilitate the ethical engagement of digital mental health interventions for service users. Finally, engaging potential end-users in the development cycle of digital mental health support platforms and increased privacy regulations may lead the field to a better understanding of effective uses of technology for people with mental health conditions. This study contributes to the ongoing debate of digital mental health ethics, data justice, and digital mental health by providing a first-hand experience of digital ethics from end-users’ perspectives.publishedVersio
CARMA Large Area Star Formation Survey: Observational Analysis of Filaments in the Serpens South Molecular Cloud
We present the N2H+(J=1-0) map of the Serpens South molecular cloud obtained
as part of the CARMA Large Area Star Formation Survey (CLASSy). The
observations cover 250 square arcminutes and fully sample structures from 3000
AU to 3 pc with a velocity resolution of 0.16 km/s, and they can be used to
constrain the origin and evolution of molecular cloud filaments. The spatial
distribution of the N2H+ emission is characterized by long filaments that
resemble those observed in the dust continuum emission by Herschel. However,
the gas filaments are typically narrower such that, in some cases, two or three
quasi-parallel N2H+ filaments comprise a single observed dust continuum
filament. The difference between the dust and gas filament widths casts doubt
on Herschel ability to resolve the Serpens South filaments. Some molecular
filaments show velocity gradients along their major axis, and two are
characterized by a steep velocity gradient in the direction perpendicular to
the filament axis. The observed velocity gradient along one of these filaments
was previously postulated as evidence for mass infall toward the central
cluster, but these kind of gradients can be interpreted as projection of
large-scale turbulence.Comment: 12 pages, 4 figures, published in ApJL (July 2014
CARMA Large Area Star Formation Survey: Project Overview with Analysis of Dense Gas Structure and Kinematics in Barnard 1
We present details of the CARMA Large Area Star Formation Survey (CLASSy),
while focusing on observations of Barnard 1. CLASSy is a CARMA Key Project that
spectrally imaged N2H+, HCO+, and HCN (J=1-0 transitions) across over 800
square arcminutes of the Perseus and Serpens Molecular Clouds. The observations
have angular resolution near 7" and spectral resolution near 0.16 km/s. We
imaged ~150 square arcminutes of Barnard 1, focusing on the main core, and the
B1 Ridge and clumps to its southwest. N2H+ shows the strongest emission, with
morphology similar to cool dust in the region, while HCO+ and HCN trace several
molecular outflows from a collection of protostars in the main core. We
identify a range of kinematic complexity, with N2H+ velocity dispersions
ranging from ~0.05-0.50 km/s across the field. Simultaneous continuum mapping
at 3 mm reveals six compact object detections, three of which are new
detections. A new non-binary dendrogram algorithm is used to analyze dense gas
structures in the N2H+ position-position-velocity (PPV) cube. The projected
sizes of dendrogram-identified structures range from about 0.01-0.34 pc.
Size-linewidth relations using those structures show that non-thermal
line-of-sight velocity dispersion varies weakly with projected size, while rms
variation in the centroid velocity rises steeply with projected size. Comparing
these relations, we propose that all dense gas structures in Barnard 1 have
comparable depths into the sky, around 0.1-0.2 pc; this suggests that
over-dense, parsec-scale regions within molecular clouds are better described
as flattened structures rather than spherical collections of gas. Science-ready
PPV cubes for Barnard 1 molecular emission are available for download.Comment: Accepted to The Astrophysical Journal (ApJ), 51 pages, 27 figures
(some with reduced resolution in this preprint); Project website is at
http://carma.astro.umd.edu/class
Fast DNA translocation through a solid-state nanopore
We report translocation experiments on double-strand DNA through a silicon
oxide nanopore. Samples containing DNA fragments with seven different lengths
between 2000 to 96000 basepairs have been electrophoretically driven through a
10 nm pore. We find a power-law scaling of the translocation time versus
length, with an exponent of 1.26 0.07. This behavior is qualitatively
different from the linear behavior observed in similar experiments performed
with protein pores. We address the observed nonlinear scaling in a theoretical
model that describes experiments where hydrodynamic drag on the section of the
polymer outside the pore is the dominant force counteracting the driving. We
show that this is the case in our experiments and derive a power-law scaling
with an exponent of 1.18, in excellent agreement with our data.Comment: 5 pages, 2 figures. Submitted to PR
A multi-wavelength analysis for interferometric (sub-)mm observations of protoplanetary disks: radial constraints on the dust properties and the disk structure
Theoretical models of grain growth predict dust properties to change as a
function of protoplanetary disk radius, mass, age and other physical
conditions. We lay down the methodology for a multi-wavelength analysis of
(sub-)mm and cm continuum interferometric observations to constrain
self-consistently the disk structure and the radial variation of the dust
properties. The computational architecture is massively parallel and highly
modular. The analysis is based on the simultaneous fit in the uv-plane of
observations at several wavelengths with a model for the disk thermal emission
and for the dust opacity. The observed flux density at the different
wavelengths is fitted by posing constraints on the disk structure and on the
radial variation of the grain size distribution. We apply the analysis to
observations of three protoplanetary disks (AS 209, FT Tau, DR Tau) for which a
combination of spatially resolved observations in the range ~0.88mm to ~10mm is
available (from SMA, CARMA, and VLA), finding evidence of a decreasing maximum
dust grain size (a_max) with radius. We derive large a_max values up to 1 cm in
the inner disk between 15 and 30 AU and smaller grains with a_max~1 mm in the
outer disk (R > 80AU). In this paper we develop a multi-wavelength analysis
that will allow this missing quantity to be constrained for statistically
relevant samples of disks and to investigate possible correlations with disk or
stellar parameters.Comment: 19 pages, 15 figures, accepted for publication in A&
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