Influence of bioturbation on denitrification activity in Mediterranean coastal sediments:an in situ experimental approach

An in situ experiment was conducted in the French Mediterranean littoral (Gulf of Fos) from July 1993 to January 1994 using controls without macrofauna or natural sediments. After 1, 4 and 6 mo, sediment reworking and denitrification activities (natural and potential rates) were studied. The bacterial processes were stimulated by the bioturbating activity of the autochthonous infauna. The natural and potential denitrification rates were 160 and 280% higher, respectively, than in the controls. The increase of denitrification, occurring at different depths in the sediment with respect to time, was directly dependent on the macrofaunal activity

Effects of population density on the sediment mixing induced by the gallery-diffusor Hediste (Nereis) diversicolor O.F. Müller, 1776

The aim of this work was to quantify the intensity of sediment mixing induced by the gallery-diffusor (functional bioturbation group) Hediste diversicolor as a function of density, using particles tracers (luminophores). In order to assess the impact of density on sediment reworking, a 1-D model was used to obtain sediment reworking coefficients such as Db (biodiffusion-like) and r (biotransport). Densities used in this experiment corresponded to population densities observed in the sampling area (Saint-Antoine Canal, Gulf of Fos, France): 144, 288, 577, 1153 indiv/m2. At first, results showed that neither luminophore maximum burying depth nor the more marked tracer accumulation areas were influenced by density. Thus density did not seem to have any influence on size of galleries or complexity of structure. Then, density-dependent relations with Db (biodiffusion-like mixing) and r (biotransport) were highlighted with an observed process intensity rate twice as high at highest worm density. On the other hand, Db and r per capita coefficients were negatively influenced by density. Db and r per capita at highest density were equal to ∼20% of individual Db and r obtained at the lowest density. Finally, this study showed the importance of density which appears to be a key parameter in the functioning of the sedimentary ecosystem

Comparison between the Nereis diversicolor and Nereis virens marine worms in the transformation of ingested hydrocarbons

A feeding experiment was conducted on the marine worm Nereis diversicolor to compare the fate of a hydrocarbon mixture during the gut passage in this species with the hydrocarbon breakdown process demonstrated for Nereis virens. Hydrocarbon dissolution/solubilization processes in the gut of N. diversicolor were found to have similar qualitative and quantitative importance in the hydrocarbon transformation as those observed in N. virens

Functional response of an adapted subtidal macrobenthic community to an oil spill: macrobenthic structure and bioturbation activity over time throughout an 18-month field experiment

An experimental oil spill was carried out in order toassess in situ responses of a macrobenthic community of shallow subtidal sediments historically exposed to petroleum contamination. Both structural and functional (bioturbation activity) parameters of the community, subjected or not to a pulse acute contamination (25,000 ppm), were studied for 18 months. No difference in the community structure was detected between contaminated and control sediments, from 6 to 18 months of experimentation. Vertical distributions of organisms, however, were affected by the presence of oil contamination leading to a deeper burial of some polychaete species. In the same time, changes in sediment-reworking activity and more especially a deeper particle burying in sediments subjected to acute oil contamination were shown. These results highlight the need to complete the analysis of community structure by assessing functional aspects, such as bioturbation activity, a process integrating various aspects of benthic behaviour (e.g. feeding, locomotion, burrow building)in order to estimate real (structural and functional) and longterm effects of oil contamination on benthic communities

Benthic macrofauna and sediment reworking quantification in contrasted environments in the Thau Lagoon

As part of the Microbent-PNEC Program: ‘‘Biogeochemical processes at the wateresediment interface in eutrophicated environment’’, the aim of this work was to specifically investigate and quantify the relationships between macrobenthos and sediment reworking in the Thau Lagoon in order to provide information on the potential contaminant distribution and movements at the wateresediment interface. In order to achieve this, three cores were sampled at two stations (in the central part of the Thau Lagoon and near the shellfish farming zone) in the Thau Lagoon, in December 2001, April 2002, August 2002, January 2003 and May 2003. On the basis of quantification of macrobenthos and sediment reworking, evidence is provided of: (1) similar sediment mixing intensities for different species composition at the two stations; (2) the major role of functional bioturbation groups (e.g., biodiffusors and gallery-diffusors) modulated by seasonal variability on sediment mixing; (3) an increase of intensity in summer suggesting potentially different patterns of redistribution, bioaccumulation and chemical fate (e.g., speciation) of deposited contaminants

Influence of Chironomus riparius (Diptera, Chironomidae) and Tubifex tubifex (Annelida, Oligochaeta) on oxygen uptake by sediments. Consequences of uranium contamination

The diffusive oxygen uptake (DOU) of sediments inhabited by Chironomus riparius and Tubifex tubifex was investigated using a planar oxygen optode device, and complemented by measurements of bioturbation activity. Additional experiments were performed within contaminated sediments to assess the impact of uranium on these processes. After 72 h, the two invertebrate species significantly increased the DOU of sediments (13–14%), and no temporal variation occurred afterwards. Within contaminated sediments, it was already 24% higher before the introduction of the organisms, suggesting that uranium modified the sediment biogeochemistry. Although the two species firstly reacted by avoidance of contaminated sediment, they finally colonized it. Their bioturbation activity was reduced but, for T. tubifex, it remained sufficient to induce a release of uranium to the water column and an increase of the DOU (53%). These results highlight the necessity of further investigations to take into account the interactions between bioturbation, microbial metabolism and pollutants

Hydrocarbon influence on denitrification in bioturbated Mediterranean coastal sediments

An in situ experiment has been carried out inbioturbated Mediterranean coastal marine sediments (Gulfof Fos) in order to study the influence of hydrocarbons ondenitrification after 1, 4 and 6 months. In theabsence of hydrocarbons in the control sediments, the presenceof macrofauna stimulated denitrificationby 160%. This stimulation is induced by sediment reworkingthat favours both direct NO-3 supply fromthe water column and the penetration of O{2}, which in turnstimulated nitrification, the other source ofNO-3 in sediment. The presence of hydrocarbons in theexperimental sediments either stimulated orinhibited the denitrification. The denitrification response tothe presence of hydrocarbon is dependent onthe quantity of matter buried by the macrofauna activity. Insmall quantities, the organic matter relatedto hydrocarbons 120% enhanced the denitrification compared tothe controls. On the other hand, whenburied hydrocarbon concentrations were higher (>100 mgsaturated hydrocarbon fraction kg-1 drysediment), the denitrification was inhibited.On the basis of the results obtained, a descriptive model ofthe patterns of denitrification in relation to the presence ofmacrofauna and the distribution of hydrocarbons in sediments is proposed

Influence of bioturbation by the polychaete Nereis diversicolor on the structure of bacterial communities in oil contaminated coastal sediments

Patterns of change in the structure of bacterial communities monitored by ribosomal intergenic spacer analysis (RISA) in oil contaminated sediments inhabited or not by the marine polychaete Nereis diversicolor were studied during 45 days under laboratory conditions. Results supported by principal component analysis showed a marked response of the bacterial communities to the oil contamination and to the presence of N. diversicolor. Phylogenetic affiliation of specific RISA bands showed that, in the contaminated sediments, the presence of the marine polychaetes favoured the development of bacteria which may play an active role in natural bioremediation processes of oil polluted environments

Inhibition of denitrification by hydrocarbons in marine sediments. Role of Nereis diversicolor

An in vitro experiment has been carried out in order to illustrate the influence of the burrowing polychaete Nereis diversicolor on denitrification in hydrocarbon-loaded sediments. After 15 and 45 days, the denitrification activity in the different contaminated or non-contaminated control (without macro-fauna) and experimental (colonized by polychaetes) sediments was compared. In control contaminated sediments, the inhibition of denitrification is solely located in the surface layer (0–2 cm). In the presence of polychaetes, the hydrocarbon buried in the burrows extends the inhibition of denitrification in the sediment. As a function of the quantity of buried hydrocarbon, the inhibition of denitrification is observed up to a depth of 6 and 10 cm, after 15 and 45 days, respectively

The deep-sea macrobenthos on the continental slope of the northwestern Mediterranean Sea: a quantitative approach

As part of the ECOMARGE operation (J.G.O.F.S. France), macrobenthic assemblages in the Toulon Canyon were described and quantified on the basis of sampling carried out between 250 and 2000 m depth on the Mediterranean continental slope. Results show that Mediterranean bathyal assemblages are made up mainly of continental shelf eurybathic species. The qualitative and quantitative composition of populations varies with depth on the slope and also varies with station position at equivalent depth, whether on the flanks or in the canyon channel. Various analyses have provided evidence on the factors responsible for this population distribution pattern. No single factor emerges as predominant, but rather a group of factors, which are related to the nature and origin of sediments and more particularly their grain size distribution, geochemical composition and mode of transportation and sedimentation (benthic nepheloid or originating from the water column), act in conjunction to determine the pattern. Comparison with ocean continental slopes shows that in the Mediterranean Sea the absence of tidal current modifies the trophic structure of the macrobenthic assemblages, which are characterized by a dominance of surface and subsurface deposit feeders as compared to a dominance of suspension feeders and carnivores in the upper and median part of the slope in the ocean. Surface dumping of dredge spoil at the canyon head and channelling of waste induces an increase of organic matter and pollutant concentrations in sediment from the upper part of the canyon channel but does not give rise to any marked population degradation