Dynamical Response of Nanomechanical Oscillators in Immiscible Viscous Fluid for in vitro Biomolecular Recognition

Dynamical response of nanomechanical cantilever structures immersed in a viscous fluid is important to in vitro single-molecule force spectroscopy, biomolecular recognition of disease-specific proteins, and the detection of microscopic dynamics of proteins. Here we study the stochastic response of biofunctionalized nanomechanical cantilevers beam in a viscous fluid. Using the fluctuation-dissipation theorem we derive an exact expression for the spectral density of the displacement and a linear approximation for the resonance frequency shift. We find that in a viscous solution the frequency shift of the nanoscale cantilever is determined by surface stress generated by biomolecular interaction with negligible contributions from mass loading.Comment: 4 pages, 2 figures, RevTex4. See http://nano.bu.edu/ for related paper

Cosmological Analogues of the Bartnik--McKinnon Solutions

We present a numerical classification of the spherically symmetric, static solutions to the Einstein--Yang--Mills equations with cosmological constant $\Lambda$. We find three qualitatively different classes of configurations, where the solutions in each class are characterized by the value of $\Lambda$ and the number of nodes, $n$, of the Yang--Mills amplitude. For sufficiently small, positive values of the cosmological constant, \Lambda < \Llow(n), the solutions generalize the Bartnik--McKinnon solitons, which are now surrounded by a cosmological horizon and approach the deSitter geometry in the asymptotic region. For a discrete set of values $\Lambda_{\rm reg}(n) > \Lambda_{\rm crit}(n)$, the solutions are topologically $3$--spheres, the ground state $(n=1)$ being the Einstein Universe. In the intermediate region, that is for \Llow(n) < \Lambda < \Lhig(n), there exists a discrete family of global solutions with horizon and ``finite size''.Comment: 16 pages, LaTeX, 9 Postscript figures, uses epsf.st

Metabolically exaggerated cardiac reactions to acute psychological stress: The effects of resting blood pressure status and possible underlying mechanisms

The study aimed to: confirm that acute stress elicits metabolically exaggerated increases in cardiac activity; test whether individuals with elevated resting blood pressure show more exaggerated cardiac reactions to stress than those who are clearly normotensive; and explore the underlying mechanisms. Cardiovascular activity and oxygen consumption were measured pre-, during, and post- mental stress, and during graded submaximal cycling exercise in 11 young men with moderately elevated resting blood pressure and 11 normotensives. Stress provoked increases in cardiac output that were much greater than would be expected from contemporary levels of oxygen consumption. Exaggerated cardiac reactions were larger in the relatively elevated blood pressure group. They also had greater reductions in total peripheral resistance, but not heart rate variability, implying that their more exaggerated cardiac reactions reflected greater β-adrenergic activation

Variable Curvature Slab Molecular Dynamics as a Method to Determine Surface Stress

A thin plate or slab, prepared so that opposite faces have different surface stresses, will bend as a result of the stress difference. We have developed a classical molecular dynamics (MD) formulation where (similar in spirit to constant-pressure MD) the curvature of the slab enters as an additional dynamical degree of freedom. The equations of motion of the atoms have been modified according to a variable metric, and an additional equation of motion for the curvature is introduced. We demonstrate the method to Au surfaces, both clean and covered with Pb adsorbates, using many-body glue potentials. Applications to stepped surfaces, deconstruction and other surface phenomena are under study.Comment: 16 pages, 8 figures, REVTeX, submitted to Physical Review

Women We Loved: Paradoxes of public and private in the biographical television drama

Broadcast to critical acclaim and relatively large audiences for its niche channel, the Women We Loved season consisted of biographical dramatisations of three prominent female figures of 20th-century British culture. These dramas shared in common narratives that centre on the two aspects of ‘the public’ and ‘the private’: the tension between public career and personal life and the discrepancy between celebrity persona and private individual. Combining theoretical insights from feminist studies of biography with close textual analysis, this article analyses how performance, aesthetics and narrative express the ambivalent placement of their protagonists between public and private spheres

GERMINAÇÃO DE SEMENTES E CRESCIMENTO INICIAL DE PLÂNTULAS DE INGÁ-MIRIM - Inga laurina (S W.) Willd – UTILIZADA NA ARBORIZAÇÃO URBANA DE RIO BRANCO, ACRE

A inga-mirim é uma espécie nativa da Floresta Amazônica com potencial para arborização urbana. Com o objetivo de estudar a germinação e crescimento inicial de plântulas desta espécie, para posterior propagação, realizou-se um experimento em casa de vegetação em temperatura ambiente e com irrigações periódicas. O delineamento experimental utilizado foi inteiramente casualizado, com quatro tratamentos e quatro repetições com 25 sementes em bandejas de plástico com 25x39x7,5 cm. Os resíduos agroflorestais testados como substrato foram casca de castanha-do-brasil triturada, casca de amendoim triturada, resíduo de açaí peneirado e areia como testemunha. Após a germinação das sementes foram analisadas porcentagem de germinação, índice de velocidade de germinação, peso fresco e seco da parte aérea e da raiz, comprimento da parte aérea e radicular, número de folhas e de folíolos. Através da análise de variância e Teste de Tukey a 5% de probabilidade verificou-se que os substratos testados proporcionaram a mesma porgentagem de germinação às sementes e índice de velocidade de germinação, porém o substrato resíduo de açaí proporcionou o melhor resultado para comprimento da parte aérea e o substrato areia para matéria verde da parte aérea, número de folhas e folíolos, mostrando-se promissor para propagação e maior vigor de plântulas desta espécie

The selection of comparators for randomized controlled trials of health-related behavioral interventions : recommendations of an NIH expert panel

Funding and logistical support for the expert panel was provided by the National Institutes of Health Office of Behavioral and Social Sciences Research.Peer reviewedPostprin

Measurement of Contractile Stress Generated by Cultured Rat Muscle on Silicon Cantilevers for Toxin Detection and Muscle Performance Enhancement

Background: To date, biological components have been incorporated into MEMS devices to create cell-based sensors and assays, motors and actuators, and pumps. Bio-MEMS technologies present a unique opportunity to study fundamental biological processes at a level unrealized with previous methods. The capability to miniaturize analytical systems enables researchers to perform multiple experiments in parallel and with a high degree of control over experimental variables for high-content screening applications.Methodology/Principal Findings: We have demonstrated a biological microelectromechanical system (BioMEMS) based on silicon cantilevers and an AFM detection system for studying the physiology and kinetics of myotubes derived from embryonic rat skeletal muscle. It was shown that it is possible to interrogate and observe muscle behavior in real time, as well as selectively stimulate the contraction of myotubes with the device. Stress generation of the tissue was estimated using a modification of Stoney's equation. Calculated stress values were in excellent agreement with previously published results for cultured myotubes, but not adult skeletal muscle. Other parameters such as time to peak tension (TPT), the time to half relaxation (KRT) were compared to the literature. It was observed that the myotubes grown on the BioMEMS device, while generating stress magnitudes comparable to those previously published, exhibited slower TPT and KRT values. However, growth in an enhanced media increased these values. From these data it was concluded that the myotubes cultured on the cantilevers were of an embryonic phenotype. The system was also shown to be responsive to the application of a toxin, veratridine.Conclusions/Significance: The device demonstrated here will provide a useful foundation for studying various aspects of muscle physiology and behavior in a controlled high-throughput manner as well as be useful for biosensor and drug discovery applications