Learning to be a Counselor at a Distance: A Qualitative Investigation of the Distance Education Experience for Counselors-in-Training

Counselor education has evolved to include distance education. There is, however, a dearth of research aimed specifically at distance education for counselors-in-training. This research looks to redress this by analyzing data gathered during interviews of six counselors-in-training. The results revealed that online counseling students were predominately satisfied with their education experience despite some concerns. Themes relating to convenience, separateness, connectedness, self-efficacy, and stigma were identified. As distance education continues to progress, it will be imperative that counselor educators create programs that meet the needs of students but also allow for proper training and protection of clients and the public

Routine human papillomavirus genotyping by DNA sequencing in community hospital laboratories

<p>Abstract</p> <p>Background</p> <p>Human papillomavirus (HPV) genotyping is important for following up patients with persistent HPV infection and for evaluation of prevention strategy for the individual patients to be immunized with type-specific HPV vaccines. The aim of this study was to optimize a robust "low-temperature" (LoTemp™) PCR system to streamline the research protocols for HPV DNA nested PCR-amplification followed by genotyping with direct DNA sequencing. The protocol optimization facilitates transferring this molecular technology into clinical laboratory practice. In particular, lowering the temperature by 10°C at each step of thermocycling during <it>in vitro </it>DNA amplification yields more homogeneous PCR products. With this protocol, template purification before enzymatic cycle primer extensions is no longer necessary.</p> <p>Results</p> <p>The HPV genomic DNA extracted from liquid-based alcohol-preserved cervicovaginal cells was first amplified by the consensus MY09/MY11 primer pair followed by nested PCR with GP5+/GP6+ primers. The 150 bp nested PCR products were subjected to direct DNA sequencing. The hypervariable 34–50 bp DNA sequence downstream of the GP5+ primer site was compared to the known HPV DNA sequences stored in the GenBank using on-line BLAST for genotyping. The LoTemp™ ready-to-use PCR polymerase reagents proved to be stable at room temperature for at least 6 weeks. Nested PCR detected 107 isolates of HPV in 513 cervicovaginal clinical samples, all validated by DNA sequencing. HPV-16 was the most prevalent genotype constituting 29 of 107 positive cases (27.2%), followed by HPV-56 (8.5%). For comparison, Digene HC2 test detected 62.6% of the 107 HPV isolates and returned 11 (37.9%) of the 29 HPV-16 positive cases as "positive for high-risk HPV".</p> <p>Conclusion</p> <p>The LoTemp™ ready-to-use PCR polymerase system which allows thermocycling at 85°C for denaturing, 40°C for annealing and 65°C for primer extension can be adapted for target HPV DNA amplification by nested PCR and for preparation of clinical materials for genotyping by direct DNA sequencing. HPV genotyping is performed by on-line BLAST algorithm of a hypervariable L1 region. The DNA sequence is included in each report to the physician for comparison in following up patients with persistent HPV infection, a recognized tumor promoter in cancer induction.</p

Cuidados biomédicos de saúde em Angola e na Companhia de Diamantes de Angola, c. 1910-1970

Pretende-se caracterizar a prestação de cuidados biomédicos em Angola durante a atividade da Companhia de Diamantes de Angola. Uma análise comparativa de políticas e práticas de saúde pública de vários atores coloniais, como os serviços de saúde da Companhia, sua congénere do Estado e outras empresas coloniais, revelará diferenças de investimento na saúde, isto é, instalações e pessoal de saúde, e tratamentos. Este escrutínio bem como as condições de vida iluminarão o carácter idiossincrático e central dos serviços de saúde da Companhia em termos de morbimortalidade em Angola, e a centralidade destes para as representações de um império cuidador

Geodemographic Disparities in Availability of Comprehensive Intimate Partner Violence Screening Services in Miami-Dade County, Florida

This study examined geodemographic factors associated with availability of comprehensive intimate partner violence (IPV) screening services in Miami-Dade County, Florida. We geocoded 2014 survey data from 278 health facilities and created a population-normalized density surface of IPV screening comprehensiveness. We used correlation analysis and spatial regression techniques to evaluate census tract-level predictors of the mean normalized comprehensiveness score (NCS) for 505 census tracts in Miami-Dade. The population-adjusted density surface of IPV screening comprehensiveness revealed geographic disparities in the availability of screening services. Using a spatial lag regression model, we observed that race and ethnicity are associated with mean NCS by census tract after controlling for age, median gross rent, and receipt of Social Security benefits. The percentage of White non-Hispanic residents was positively associated with NCS, Black non-Hispanic was negatively associated with NCS, while Hispanic—the majority ethnicity in Miami-Dade—was not associated with NCS. This exploratory study may be the first to put IPV screening comprehensiveness on the map, and provides a starting point for addressing urban disparities in the availability of IPV screening services that are shaped by race, ethnicity, zoning, and socioeconomic status

Data from: Effects of pesticide mixtures on host-pathogen dynamics of the amphibian chytrid fungus

Anthropogenic and natural stressors often interact to affect organisms. Amphibian populations are undergoing unprecedented declines and extinctions with pesticides and emerging infectious diseases implicated as causal factors. Although these factors often co-occur, their effects on amphibians are usually examined in isolation. We hypothesized that exposure of larval and metamorphic amphibians to ecologically relevant concentrations of pesticide mixtures would increase their post-metamorphic susceptibility to the fungus Batrachochytrium dendrobatidis (Bd), a pathogen that has contributed to amphibian population declines worldwide. We exposed five anuran species (Pacific treefrog, Pseudacris regilla; spring peeper, Pseudacris crucifer; Cascades frog, Rana cascadae; northern leopard frog, Lithobates pipiens; and western toad, Anaxyrus boreas) from three families to mixtures of four common insecticides (chlorpyrifos, carbaryl, permethrin, and endosulfan) or herbicides (glyphosate, acetochlor, atrazine, and 2,4-D) or a control treatment, either as tadpoles or as newly metamorphic individuals (metamorphs). Subsequently, we exposed animals to Bd or a control inoculate after metamorphosis and compared survival and Bd load. Bd exposure significantly increased mortality in Pacific treefrogs, spring peepers, and western toads, but not in Cascades frogs or northern leopard frogs. However, the effects of pesticide exposure on mortality were negligible, regardless of the timing of exposure. Bd load varied considerably across species; Pacific treefrogs, spring peepers, and western toads had the highest loads, whereas Cascades frogs and northern leopard frogs had the lowest loads. The influence of pesticide exposure on Bd load depended on the amphibian species, timing of pesticide exposure, and the particular pesticide treatment. Our results suggest that exposure to realistic pesticide concentrations has minimal effects on Bd-induced mortality, but can alter Bd load. This result could have broad implications for risk assessment of amphibians; the outcome of exposure to multiple stressors may be unpredictable and can differ between species and life stages

BdLoadData

BdLoadData.xlsx is associated with Figure 2 and Table 2

Infection level (log genome equivalents +1) for Bd-exposed amphibians exposed to pesticides as tadpoles.

<p>Species are arranged phylogenetically (rows) and by geographic origin (columns). Values plotted are means ±1SE.</p

MoralityData

MortalityData.xlsx is associated with Figure 1 and Table 1

BdLoadData

BdLoadData.xlsx is associated with Figure 2 and Table 2