The Rigidly Rotating Magnetosphere of Sigma Ori E

We attempt to characterize the observed variability of the magnetic helium-strong star sigma Ori E in terms of a recently developed rigidly rotating magnetosphere model. This model predicts the accumulation of circumstellar plasma in two co-rotating clouds, situated in magnetohydrostatic equilibrium at the intersection between magnetic and rotational equators. We find that the model can reproduce well the periodic modulations observed in the star's light curve, H alpha emission-line profile, and longitudinal field strength, confirming that it furnishes an essentially correct, quantitative description of the star's magnetically controlled circumstellar environment.Comment: 4 pages, 3 figures, accepted by Ap

The Shape and Scale of Galactic Rotation from Cepheid Kinematics

A catalog of Cepheid variables is used to probe the kinematics of the Galactic disk. Radial velocities are measured for eight distant Cepheids toward l = 300; these new Cepheids provide a particularly good constraint on the distance to the Galactic center, R_0. We model the disk with both an axisymmetric rotation curve and one with a weak elliptical component, and find evidence for an ellipticity of 0.043 +/- 0.016 near the Sun. Using these models, we derive R_0 = 7.66 +/- 0.32 kpc and v_circ = 237 +/- 12 km/s. The distance to the Galactic center agrees well with recent determinations from the distribution of RR Lyrae variables, and disfavors most models with large ellipticities at the solar orbit.Comment: 36 pages, LaTeX, 10 figure

Fundamental parameters of Cepheids. V. Additional photometry and radial velocity for southern Cepheids

I present photometric and radial velocity data for Galactic Cepheids, most of them being in the southern hemisphere. There are 1250 Geneva 7-color photometric measurements for 62 Cepheids, the average uncertainty per measurement is better than 0.01 mag. A total of 832 velocity measurements have been obtained with the CORAVEL radial velocity spectrograph for 46 Cepheids. The average accuracy of the radial velocity data is 0.38 km/s. There are 33 stars with both photometry and radial velocity data. I discuss the possible binarity or period change that these new data reveal. I also present reddenings for all Cepheids with photometry. The data are available electronically.Comment: To appear in ApJS. Data available electronically at ftp://cfa-ftp.harvard.edu/pub/dbersier

Microlensing of circumstellar envelopes III. Line profiles from stellar winds in homologous expansion

This paper examines line profile evolution due to the linear expansion of circumstellar material obsverved during a microlensing event. This work extends our previous papers on emission line profile evolution from radial and azimuthal flow during point mass lens events and fold caustic crossings. Both "flavours" of microlensing were shown to provide effective diagnostics of bulk motion in circumstellar envelopes. In this work a different genre of flow is studied, namely linear homologous expansion, for both point mass lenses and fold caustic crossings. Linear expansion is of particular relevance to the effects of microlensing on supernovae at cosmological distances. We derive line profiles and equivalent widths for the illustrative cases of pure resonance and pure recombination lines, modelled under the Sobolev approximation. The efficacy of microlensing as a diagnostic probe of the stellar environs is demonstrated and discussed

First HARPSpol discoveries of magnetic fields in massive stars

In the framework of the Magnetism in Massive Stars (MiMeS) project, a HARPSpol Large Program at the 3.6m-ESO telescope has recently started to collect high-resolution spectropolarimetric data of a large number of Southern massive OB stars in the field of the Galaxy and in many young clusters and associations. In this Letter, we report on the first discoveries of magnetic fields in two massive stars with HARPSpol - HD 130807 and HD 122451, and confirm the presence of a magnetic field at the surface of HD 105382 that was previously observed with a low spectral resolution device. The longitudinal magnetic field measurements are strongly varying for HD 130807 from $\sim$-100 G to $\sim$700 G. Those of HD 122451 and HD 105382 are less variable with values ranging from $\sim$-40 to -80 G, and from $\sim$-300 to -600 G, respectively. The discovery and confirmation of three new magnetic massive stars, including at least two He-weak stars, is an important contribution to one of the MiMeS objectives: the understanding of origin of magnetic fields in massive stars and their impacts on stellar structure and evolution.Comment: 4 pages, 2 figures, accepted for publication in A&A Lette

Magnetic fields and chemical peculiarities of the very young intermediate-mass binary system HD 72106

The recently discovered magnetic Herbig Ae and Be stars may provide qualitatively new information about the formation and evolution of magnetic Ap and Bp stars. We have performed a detailed investigation of one particularly interesting binary system with a Herbig Ae secondary and a late B-type primary possessing a strong, globally ordered magnetic field. Twenty high-resolution Stokes V spectra of the system were obtained with the ESPaDOnS instrument mounted on the CFHT. In these observations we see clear evidence for a magnetic field in the primary, but no evidence for a magnetic field in the secondary. A detailed abundance analysis was performed for both stars, revealing strong chemical peculiarities in the primary and normal chemical abundances in the secondary. The primary is strongly overabundant in Si, Cr, and other iron-peak elements, as well as Nd, and underabundant in He. The primary therefore appears to be a very young Bp star. In this context, line profile variations of the primary suggest non-uniform lateral distributions of surface abundances. Interpreting the 0.63995 +/- 0.00009 day variation period of the Stokes I and V profiles as the rotational period of the star, we have modeled the magnetic field geometry and the surface abundance distributions of Si, Ti, Cr and Fe using Magnetic Doppler Imaging. We derive a dipolar geometry of the surface magnetic field, with a polar strength of 1230 G and an obliquity of 57 degrees. The distributions Ti, Cr and Fe are all qualitatively similar, with an elongated patch of enhanced abundance situated near the positive magnetic pole. The Si distribution is somewhat different, and its relationship to the magnetic field geometry less clear.Comment: Accepted by Monthly Notices of the Royal Astronomical Society, September 2008. 15 pages, 10 figure

A dominant magnetic dipole for the evolved Ap star candidate EK Eridani

EK Eri is one of the most slowly rotating active giants known, and has been proposed to be the descendant of a strongly magnetic Ap star. We have performed a spectropolarimetric study of EK Eri over 4 photometric periods with the aim of inferring the topology of its magnetic field. We used the NARVAL spectropolarimeter at the Bernard Lyot telescope at the Pic du Midi Observatory, along with the least-squares deconvolution method, to extract high signal-to-noise ratio Stokes V profiles from a timeseries of 28 polarisation spectra. We have derived the surface-averaged longitudinal magnetic field Bl. We fit the Stokes V profiles with a model of the large-scale magnetic field and obtained Zeeman Doppler images of the surface magnetic strength and geometry. Bl variations of up to about 80 G are observed without any reversal of its sign, and which are in phase with photometric ephemeris. The activity indicators are shown to vary smoothly on a timescale compatible with the rotational period inferred from photometry (308.8 d.), however large deviations can occur from one rotation to another. The surface magnetic field variations of EK Eri appear to be dominated by a strong magnetic spot (of negative polarity) which is phased with the dark (cool) photometric spot. Our modeling shows that the large-scale magnetic field of EK Eri is strongly poloidal. For a rotational axis inclination of i = 60{\deg}, we obtain a model that is almost purely dipolar. In the dipolar model, the strong magnetic/photometric spot corresponds to the negative pole of the dipole, which could be the remnant of that of an Ap star progenitor of EK Eri. Our observations and modeling conceptually support this hypothesis, suggesting an explanation of the outstanding magnetic properties of EK Eri as the result of interaction between deep convection and the remnant of an Ap star magnetic dipole.Comment: 8 pages, 6 figures, accepted for publication in Astronomy & Astrophysic

Revisiting the Rigidly Rotating Magnetosphere model for sigma Ori E. I. Observations and Data Analysis

We have obtained 18 new high-resolution spectropolarimetric observations of the B2Vp star sigma Ori E with both the Narval and ESPaDOnS spectropolarimeters. The aim of these observations is to test, with modern data, the assumptions of the Rigidly Rotating Magnetosphere (RRM) model of Townsend & Owocki (2005), applied to the specific case of sigma Ori E by Townsend et al. (2005). This model includes a substantially offset dipole magnetic field configuration, and approximately reproduces previous observational variations in longitudinal field strength, photometric brightness, and Halpha emission. We analyze new spectroscopy, including H I, He I, C II, Si III and Fe III lines, confirming the diversity of variability in photospheric lines, as well as the double S-wave variation of circumstellar hydrogen. Using the multiline analysis method of Least-Squares Deconvolution (LSD), new, more precise longitudinal magnetic field measurements reveal a substantial variance between the shapes of the observed and RRM model time-varying field. The phase resolved Stokes V profiles of He I 5876 A and 6678 A lines are fit poorly by synthetic profiles computed from the magnetic topology assumed by Townsend et al. (2005). These results challenge the offset dipole field configuration assumed in the application of the RRM model to sigma Ori E, and indicate that future models of its magnetic field should also include complex, higher-order components.Comment: 13 pages, 8 figures. Accepted for publication in MNRA

Magnetic field topology and chemical spot distributions of the Ap star HD119419

Analysis of high-resolution spectropolarimetric observations of early-type magnetic stars is currently the most advanced method of obtaining detailed information on their surface magnetic field topologies and horizontal spot distributions. In this study we analyse a new set of high-quality four Stokes parameter observations of the magnetic Ap star HD119419 - a member of the 14 Myr old Lower Cen-Cru association - for the purpose of studying the surface field topology and mapping the chemical abundance spots. We made use of the circular and linear polarisation data collected for HD119419 with the HARPSpol instrument at the ESO 3.6-m telescope. These observations were analysed with a multi-line magnetic diagnostic technique and modelled in detail with a Magnetic Doppler imaging code. We present a new set of mean longitudinal magnetic field measurements and derive a revised stellar rotational period. We also redetermine the basic stellar atmospheric parameters. Our four Stokes parameter magnetic inversions reveal a moderately complex surface field topology with a mean field strength of 18 kG and a maximum local strength of 24 kG. A poloidal dipolar component dominates the magnetic energy spectrum of the surface field in HD119419. However, significant contributions of the higher-order spherical harmonic components are also present. We show that the dipole plus quadrupole part of the reconstructed field geometry is incapable of reproducing the observed amplitudes and shapes of the Stokes Q and U profiles. The chemical abundance distributions of Fe, Cr, Ti, and Nd, derived self-consistently with the magnetic field geometry, are characterised by large abundance gradients and a lack of clear correlation with the magnetic field structure. This full Stokes vector analysis of HD119419 extends the modern hot-star magnetic mapping investigations to an open cluster Ap star with a well-determined age.Comment: 15 pages, 11 figures; accepted for publication in A&

Scalable manufacturing of gene-modified human mesenchymal stromal cells with microcarriers in spinner flasks

Due to their immunomodulatory properties and in vitro differentiation ability, human mesenchymal stromal cells (hMSCs) have been investigated in more than 1000 clinical trials over the last decade. Multiple studies that have explored the development of gene-modified hMSC-based products are now reaching early stages of clinical trial programmes. From an engineering perspective, the challenge lies in developing manufacturing methods capable of producing sufficient doses of ex vivo gene-modified hMSCs for clinical applications. This work demonstrates, for the first time, a scalable manufacturing process using a microcarrier-bioreactor system for the expansion of gene-modified hMSCs. Upon isolation, umbilical cord tissue mesenchymal stromal cells (UCT-hMSCs) were transduced using a lentiviral vector (LV) with green fluorescent protein (GFP) or vascular endothelial growth factor (VEGF) transgenes. The cells were then seeded in 100 mL spinner flasks using Spherecol microcarriers and expanded for seven days. After six days in culture, both non-transduced and transduced cell populations attained comparable maximum cell concentrations (≈1.8 × 105 cell/mL). Analysis of the culture supernatant identified that glucose was fully depleted after day five across the cell populations. Lactate concentrations observed throughout the culture reached a maximum of 7.5 mM on day seven. Immunophenotype analysis revealed that the transduction followed by an expansion step was not responsible for the downregulation of the cell surface receptors used to identify hMSCs. The levels of CD73, CD90, and CD105 expressing cells were above 90% for the non-transduced and transduced cells. In addition, the expression of negative markers (CD11b, CD19, CD34, CD45, and HLA-DR) was also shown to be below 5%, which is aligned with the criteria established for hMSCs by the International Society for Cell and Gene Therapy (ISCT). This work provides a foundation for the scalable manufacturing of gene-modified hMSCs which will overcome a significant translational and commercial bottleneck. KEY POINTS: • hMSCs were successfully transduced by lentiviral vectors carrying two different transgenes: GFP and VEGF • Transduced hMSCs were successfully expanded on microcarriers using spinner flasks during a period of 7 days • The genetic modification step did not cause any detrimental impact on the hMSC immunophenotype characteristics