The Grizzly, February 26, 1988

Art Expo • Patterns Campaign Nears Completion • Is He a Dummy or Isn\u27t He? • Patterns Campaign Nears Completion • Editorial: Boo! Hiss! to Prof. Epistle • Letter: Doughty to Grizzly Editor - Kiss Off; Get a Room; Cookbooks Stew Students • Zimmers Open Hearts • Curious George to the Rescue • Teams Sport Banner Seasons • Lady Bears Net Successful Record • Wrestlers Reaching Peak • The Grizzly Proudly Salutes Our Bear Pack Champions • Bears Making Tracks • Harrison Floating on Cloud Nine • Ensemble Enchanting • Projected Art Center Plans • Air Band Acts Wow Wismer Crowdhttps://digitalcommons.ursinus.edu/grizzlynews/1206/thumbnail.jp

The actinobacterial transcription factor RbpA binds to the principal sigma subunit of RNA polymerase

RbpA is a small non-DNA-binding transcription factor that associates with RNA polymerase holoenzyme and stimulates transcription in actinobacteria, including Streptomyces coelicolor and Mycobacterium tuberculosis. RbpA seems to show specificity for the vegetative form of RNA polymerase as opposed to alternative forms of the enzyme. Here, we explain the basis of this specificity by showing that RbpA binds directly to the principal σ subunit in these organisms, but not to more diverged alternative σ factors. Nuclear magnetic resonance spectroscopy revealed that, although differing in their requirement for structural zinc, the RbpA orthologues from S. coelicolor and M. tuberculosis share a common structural core domain, with extensive, apparently disordered, N- and C-terminal regions. The RbpA-σ interaction is mediated by the C-terminal region of RbpA and σ domain 2, and S. coelicolor RbpA mutants that are defective in binding σ are unable to stimulate transcription in vitro and are inactive in vivo. Given that RbpA is essential in M. tuberculosis and critical for growth in S. coelicolor, these data support a model in which RbpA plays a key role in the σ cycle in actinobacteria

The Grizzly, February 10, 1989

Ursinus Seeks Mid-States Reaccreditation • Heck Beats Traffic Blahs • Letter: Doughty Expresses Doubts • U.C. Salutes French Bicentennial • Medieval Fest Needs You • Ginsberg to Give Revolutionary Forum • Casa Maria: Muy Bien • de la Hoya Happy • Ursinus Slays F & M • U.C. Aims for Title • O\u27Malley Leaps to Nationals • Women\u27s Indoor Inspiring • U.C. Fields Strong Squad • A\u27Bears Peaking at Right Time • Scholarships to Scotland • Guess Who\u27s Coming for Dinner? • His Cheating Makes Twice the Test for You • Hallinger Argues for Proposal 42: Academics Before Athlete • Greenstein Grabs Grim \u27Just Right\u27 • Valentine No-No\u27s • From America With Love: Students Flock to U.C.https://digitalcommons.ursinus.edu/grizzlynews/1228/thumbnail.jp

Pulsed Fission Fusion (PuFF) Propulsion System

No abstract availabl