Heatmaps for Patterns of Association in log-Linear Models:

Log-linear models offer a detailed characterization of the association between categorical variables, but the breadth of their outputs is difficult to grasp because of the large number of parameters these models entail. Revisiting seminal findings and data from sociological work on social mobility, the author illustrates the use of heatmaps as a visualization technique to convey the complex patterns of association captured by log-linear models. In particular, turning log odds ratios derived from a model's predicted counts into heatmaps makes it possible to summarize large amounts of information and facilitates comparison across models' outcomes

Freeze-thawing of liposomes: investigation of cryoprotectants for freeze-drying

Liposomes are phospholipid membranes widely used for drug delivery in biomedical applications. They can retain, protect and deliver macromolecules to target tissues and then control the release of their cargoes. To exploit this function, the liposomal membrane integrity is crucial to avoid undesired leakage of cargoes. In this work, the influence of low temperature storing conditions on the stability of liposomes is investigated for further delivery applications

Eosinophils Target Therapy for Severe Asthma: Critical Points

Asthma is a chronic and heterogeneous disease, which is defined as severe disease whenever it requires treatment with a high dose of inhaled corticosteroids plus a second controller and/or systemic corticosteroids to prevent it from becoming ‘‘uncontrolled’’ or if it remains ‘‘uncontrolled’’ despite this therapy. Severe asthma is a heterogeneous condition consisting of phenotypes such as eosinophilic asthma, which is characterized by sputum eosinophilia, associated with mild to moderate increase in blood eosinophil count, frequently adult-onset, and associated with chronic rhinosinusitis with nasal polyps in half of the cases. Eosinophilic asthma is driven by T2 inflammation, characterized, among the others, by interleukin-5 production. IL-5 plays a key role in the differentiation, survival, migration, and activation of eosinophils, and it has become an appealing therapeutic target for eosinophilic asthma. In recent years two monoclonal antibodies (mepolizumab and reslizumab) directed against IL-5 and one monoclonal antibody directed against the alpha-subunit of the IL-5 receptor (benralizumab) have been developed. All these IL-5 target drugs have been shown to reduce the number of exacerbation in patients with severe asthma selected on the basis of peripheral blood eosinophil count. There are still a number of unresolved issues related to the anti-IL5 strategy in eosinophilic asthma, which are here reviewed. These issues include the effects of such therapy on airway obstruction and asthmatic symptoms, the level of baseline eosinophils that predicts a response to treatment, the relationship between blood and airway eosinophilia, and, perhaps most importantly, how to elucidate the pathogenetic role played by eosinophils in the individual patient with severe eosinophilic asthma

Fraintendimenti e intendimenti. Le interazioni comunicative nativi/non nativi, tra asimmetrie e cooperazione

Il focus della ricerca sono le interazioni comunicativi tra nativi e non nativi. La ricerca è svolta attraverso l’osservazione e analisi di interazioni comunicative in 3 contesti differenti, nella città di Palermo; esplora l’influenza di pregiudizi e stereotipi su questi processi, anche a livello inconsapevole, nelle interazioni quotidiane. L’attenzione si centra nell’individuare segnali comunicativi e modalità linguistiche, verbali e non verbali agiti in contesti istituzionalmente destinati all’accoglienza ed a favorire processi di integrazione. Obiettivo è di verificare se, e in quale misura, la qualità dell’interazione tra i soggetti coinvolti sia coerente con gli intenti esplicitamente dichiarati o se, invece, agisca in direzione diverseThe focus of the research are the communicative interactions between native and non-native people. The research is carried out through observations and analysis of communicative interactions in 3 different contexts, in the city of Palermo; it explores the influence of prejudices and stereotypes about these processes, even at an unconscious level, in everyday interactions. The attention is focused in identifying signals and linguistic modes of communication, verbal and non-verbal acting out in institutional contexts for the reception and to foster integration. The objective is to determine whether the quality of interaction between the parties involved is consistent with the intent explicitly stated or if, instead, act in different direction

Trabas a las importaciones y sus consecuencias en la provincia de Mendoza desde 2012 hasta 2015

El presente trabajo de investigación, pretende analizar el impacto ocasionado en los distintos sectores económicos de la Provincia de Mendoza, a raíz de las diversas restricciones a las importaciones por el Gobierno Nacional, durante el período 2012-2015.Fil: Bucca Trejo, Leandro Ezequiel. Universidad Nacional de Cuyo. Facultad de Ciencias Económicas.Fil: Iniesta Di Cesare, Mariano Juan. Universidad Nacional de Cuyo. Facultad de Ciencias Económicas.Fil: Reggio, Pablo Alejandro. Universidad Nacional de Cuyo. Facultad de Ciencias Económicas

Release of Type 2 Cytokines by Epithelial Cells of Nasal Polyps

Background. T2 inflammation of chronic rhinosinusitis with nasal polyps (CRSwNP) may be influenced by epithelial cytokines release (TSLP, IL-25, and IL-33). We investigated the release of TSLP, IL-25, and IL-33 by epithelial CRSwNP cells compared to epithelial sinus mucosa cells of patients with chronic rhinosinusitis without nasal polyps (CRSsNP). Methods. IL-25, IL-33, and TSLP were measured by ELISA in the supernatant of cell cultures derived by CRSwNP (9 patients, 6 atopic) and CRSsNP (7 patients, 2 atopic) in baseline condition and following stimulation with Dermatophagoides pteronyssinus (DP), Aspergillus fumigatus (AF), and poly(I:C). Results. CRSwNP epithelial cells released increased levels of IL-25 (from 0.12 ± 0.06 pg/ml to 0.27 ± 0.1 pg/ml, p<0.01) and TSLP (from 0.77 ± 0.5 pg/ml to 2.53 ± 1.17 pg/ml, p<0.001) following poly(I:C) stimulation, while CRSsNP epithelial cells released increased levels of IL-25 and IL-33 following AF and DP stimulation, respectively (IL-25: from 0.18 ± 0.07 pg/ml to 0.51 ± 0.1 pg/ml, p<0.001; IL-33: from 2.57 ± 1.3 pg/ml to 5.7 ± 3.1 pg/ml, p<0.001). Conclusions. CRSwNP epithelial cells release TSLP and IL-25 when stimulated by poly(I:C) but not by DP or AF, suggesting that viral infection may contribute to maintain and amplify the T2 immune response seen in CRSwNP

Vitamins D3 and D2 have marked but different global effects on gene expression in a rat oligodendrocyte precursor cell line

Background: Vitamin D deficiency increases the risk of developing multiple sclerosis (MS) but it is unclear whether vitamin D supplementation improves the clinical course of MS, and there is uncertainty about the dose and form of vitamin D (D2 or D3) to be used. The mechanisms underlying the effects of vitamin D in MS are not clear. Vitamin D3 increases the rate of differentiation of primary oligodendrocyte precursor cells (OPCs), suggesting that it might help remyelination in addition to modulating the immune response. Here we analyzed the transcriptome of differentiating rat CG4 OPCs treated with vitamin D2 or with vitamin D3 at 24 h and 72 h following onset of differentiation. Methods: Gene expression in differentiating CG4 cells in response to vitamin D2 or D3 was quantified using Agilent DNA microarrays (n=4 replicates), and the transcriptome data were processed and analysed using the R software environment. Differential expression between the experimental conditions was determined using LIMMA, applying the Benjamini and Hochberg multiple testing correction to p-values, and significant genes were grouped into co-expression clusters by hierarchical clustering. The functional significance of gene groups was explored by pathway enrichment analysis using the clusterProfiler package. Results: Differentiation alone changed the expression of about 10% of the genes at 72 h compared to 24 h. Vitamin D2 and D3 exerted different effects on gene expression, with D3 influencing 1,272 genes and D2 574 at 24 h. The expression of the vast majority of these genes was either not changed in differentiating cells not exposed to vitamin D or followed the same trajectory as the latter. D3-repressed genes were enriched for Gene Ontology (GO) categories including transcription factors and the Notch pathway, while D3-induced genes were enriched for the Ras pathway. Conclusions: This study shows that vitamin D3, compared with D2, changes the expression of a larger number of genes in OLs. Identification of genes affected by D3 in OLs should help to identify mechanisms mediating its action in MS

BEHAVIOUR OF Aeromonas hydrophila IN SALTED SWORDFISH SAMPLES

A challenge test for Aeromonas hydrophila in salted swordfish samples was carried out. Particularly, 24 samples (250g) were experimentally contaminated, salted and stored at two different temperature regimes (fluctuating – F group - and non fluctuating – NF group – regime). The count of A. hydrophila, Enterobacteria and Lactic acid bacteria (LAB) as well as the determination of pH and aw were performed at 0, 19 43, 163, 187, 230, 320 and 368 hours whereas the temperature was monitored continuously by using 6 data-loggers. In both group, the mean concentrations of A. hydrophila did not exceed Log 3 cfu/g and decreased below the mean value of Log 1 cfu/g after 368 hours. However in the F group the A. hydrophila growth was slower and the decrease appeared slightly higher than NF group and this suggests the temperature fluctuations induces a more pronounced behaviour variability of A. hydrophila under stressing conditions

ELECTROMECHANICAL INTERACTION BETWEEN CARBON BASED PANTOGRAPH STRIP AND COPPER CONTACT WIRE: A HEURISTIC WEAR MODEL

Main contributions to the wear in the sliding contact between pantograph's strip and catenary's wire can be classified as: i) mechanical contribution, due to friction, ii) electrical contribution, due to current flow at the contact and iii) electrical arcs contribution related to power dissipated during arc generation. In this work, a heuristic wear model for the contact wire, which accounts for the mentioned three main contributions to the wear, is presented. After a tuning phase with results obtained by an experimental campaign aimed at evaluating the wear for the couple “pure copper contact wire – Kasperovski contact strip”, the wear model is used in combination with a dynamical electromechanical model able to reproduce the electromechanical pantograph-catenary interaction