4 research outputs found
Thorough investigation of the phenolic profile of reputable Greek honey varieties:varietal discrimination and floral markers identification using liquid chromatography–high-resolution mass spectrometry
Honey is a highly consumed commodity due to its potential health benefits upon certain consumption, resulting in a high market price. This fact indicates the need to protect honey from fraudulent acts by delivering comprehensive analytical methodologies. In this study, targeted, suspect and non-targeted metabolomic workflows were applied to identify botanical origin markers of Greek honey. Blossom honey samples (n = 62) and the unifloral fir (n = 10), oak (n = 24), pine (n = 39) and thyme (n = 34) honeys were analyzed using an ultra-high-performance liquid chromatography hybrid quadrupole time-of-flight mass spectrometry (UHPLC-q-TOF-MS) system. Several potential authenticity markers were revealed from the application of different metabolomic workflows. In detail, based on quantitative targeted analysis, three blossom honey markers were found, namely, galangin, pinocembrin and chrysin, while gallic acid concentration was found to be significantly higher in oak honey. Using suspect screening workflow, 12 additional bioactive compounds were identified and semi-quantified, achieving comprehensive metabolomic honey characterization. Lastly, by combining non-targeted screening with advanced chemometrics, it was possible to discriminate thyme from blossom honey and develop binary discriminatory models with high predictive power. In conclusion, a holistic approach to assessing the botanical origin of Greek honey is presented, highlighting the complementarity of the three applied metabolomic approaches
NPHS2 screening with SURVEYOR in Hellenic children with steroid-resistant nephrotic syndrome
Joint ESH excellence centers' national meeting on renal sympathetic denervation: A Greek experts' survey
Objective: The efficacy of renal sympathetic denervation (RDN) has been
affirmed by a number of recent clinical studies, despite controversies
in this field over the last five years. Therefore, it is of paramount
importance that hypertension experts debate the merits of RDN by
revealing and expressing their personal beliefs and perspectives
regarding this procedure. Methods: A cross-sectional survey was
conducted among Greek leaders of the Hypertension Excellence Centers
with the use of a closed-type questionnaire specifically designed to
elicit information and evaluate the respondent’s views and perspectives
about RDN efficacy, safety and ideal target patient population. Results:
A total of 36 participants completed the survey. Based on the results,
RDN was considered efficient (91.7%) and safe (94.5%), while the
overwhelming majority of the participants felt confident in the
long-term efficacy (88.9%) of the intervention and that it lacks
reliable predictors of blood pressure response (94.5%). Patients with
resistant (91.7%), ultra-resistant (94.4%), and uncontrolled
hypertension (80.6%) were suggested as ideal candidates for RDN.
Establishing a close co-operation between interventionalists and
hypertension experts was considered essential to ensure the efficacy
(97.2%) as well as the safety (97.3%) of the procedure. Conclusion:
The vast majority of Greek hypertension experts surveyed were convinced
of the efficacy and safety of RDN based on the preponderance of
available scientific and clinical data. Identification of the ideal
patient group remains controversial. Respondents generally agreed on the
necessity of building close collaborative relationships between
interventionalists and hypertension experts in order to improve RDN
clinical outcome. (c) 2021 Hellenic Society of Cardiology. Publishing
services by Elsevier B.V. This is an open access article under the CC
BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)