PraQ-A Scale Handbook: Students' Learning Growth Evaluation of scientific experimental labs

Scale Handbook for PraQ-A Questionnaire for Laboratory quality described in Publications by Daniel Rehfeldt. For full information about the theoretical model behind the instrument and test evaluation studies ("validity"), see: Rehfeldt, D. (2018). Erfassung der Lehrqualität naturwissenschaftlicher Experimentalpraktika – Dissertationsschrift (Band 246). Berlin: Logos

The First Fifty Years of the History of the Igreja Evangelica Lutherana Do Brazil, The Brazilian District of the Missouri Synod

The present study is an attempt to describe and to analyze the main developments of the work of the Missouri Synod in Brazil during the first half of the twentieth century, from the beginning of its missionary- work in Brazil in 1900 to 1950

Sulfo-SMCC Prevents Annealing of Taxol-Stabilized Microtubules In Vitro

Microtubule structure and functions have been widely studied in vitro and in cells. Research has shown that cysteines on tubulin play a crucial role in the polymerization of microtubules. Here, we show that blocking sulfhydryl groups of cysteines in taxol-stabilized polymerized microtubules with a commonly used chemical crosslinker prevents temporal end-to-end annealing of microtubules in vitro. This can dramatically affect the length distribution of the microtubules. The crosslinker sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate, sulfo-SMCC, consists of a maleimide and an N-hydroxysuccinimide ester group to bind to sulfhydryl groups and primary amines, respectively. Interestingly, addition of a maleimide dye alone does not show the same interference with annealing in stabilized microtubules. This study shows that the sulfhydryl groups of cysteines of tubulin that are vital for the polymerization are also important for the subsequent annealing of microtubules.Comment: 3 figure

The Filament Sensor for Near Real-Time Detection of Cytoskeletal Fiber Structures

A reliable extraction of filament data from microscopic images is of high interest in the analysis of acto-myosin structures as early morphological markers in mechanically guided differentiation of human mesenchymal stem cells and the understanding of the underlying fiber arrangement processes. In this paper, we propose the filament sensor (FS), a fast and robust processing sequence which detects and records location, orientation, length and width for each single filament of an image, and thus allows for the above described analysis. The extraction of these features has previously not been possible with existing methods. We evaluate the performance of the proposed FS in terms of accuracy and speed in comparison to three existing methods with respect to their limited output. Further, we provide a benchmark dataset of real cell images along with filaments manually marked by a human expert as well as simulated benchmark images. The FS clearly outperforms existing methods in terms of computational runtime and filament extraction accuracy. The implementation of the FS and the benchmark database are available as open source.Comment: 32 pages, 21 figure

The Other Sheep Volume 40 Number 02

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The Other Sheep Volume 42 Number 10

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The Other Sheep Volume 41 Number 01

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The Other Sheep Volume 37 Number 12

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The Other Sheep Volume 39 Number 05

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