Einsatz mikrobiologischer Präparate zur Regulierung von Schadinsekten und Krankheiten an Erdbeeren

Im organischen Erdbeeranbau sind bodenbürtige Krankheiten, z. B. Verticillium-Welke und Rhizomfäule, ein wachsendes Problem. Auch im konventionellen Anbau ist eine Bekämpfung der Verticillium-Welke nur bedingt möglich, da keine effizienten Pflanzenschutzmittel verfügbar sind. Aus diesem Grund sollte untersucht werden, ob Mikroorganismen zur Regulierung von bodenbürtigen Krankheiten an Erdbeeren verwendet werden können. Hierfür wurden 98 Mikroorganismen, davon 68 Bakterien und 26 Pilze, in in vitro Tests gegen die Verticillium-Welke (V. albo-atrum, V. dahliae), die Rhizomfäule (Phytophthora cactorum) und die Rote Wurzelfäule getestet. In einem mehrstufigen Selektionsprozess wurden von den 98 Mikroorganismen 15 Mikroorganismen für weitere Laborversuche ausgewählt. Als Selektionskriterien wurden u.a. das antagonistische Potential, die Kombinierbarkeit und die Marktverfügbarkeit berücksichtigt. Die fünfzehn Mikroorganismen, die die vorgegebenen Kriterien erfüllten, wurden in weiteren Kompatibilitätstest getestet. Anschließend wurden vier Mikroorganismen ausgewählt: zwei Trichoderma (T. atroviride P1 und T. harzianum T58), ein entomopathogener Pilz (Metarhizium anisopliae Ma43) und ein Bakterium (Bacillus subtilis FZB24). Diese Antagonisten wurden in Gewächshaus- und Freilandversuche an zwei Erdbeersorten (Honeoye und Sonata) in mit V. dahliae und P. cactorum inokulierter Erde getestet. Weder in Gewächshaus- noch in Freilandversuchen konnten auch nach künstlicher Inokulation des Bodens mit den zwei Pathogenen reproduzierbare Krankheitssymptome hervorgerufen werden. Damit konnte keine klare Aussage zur befallsreduzierenden Wirkung der antagonistischen Mikroorganismen getroffen werden. Allerdings wurde in Gewächshausversuchen mit V. dahliae inokulierter Erde bei der Sorte Honeoye ein positiver Einfluss der Antagonisten auf unterschiedliche Wachstumsparameter beobachtet. Die Ergebnisse zum Einfluss der Antagonisten auf die Mikrosklerotien, die Überdauerungsformen von V. dahliae, waren widersprüchlich. Nur in Freilandversuchen wurde eine Reduktion der Mikrosklerotien durch pilzliche Antagonisten erreicht

A responsabilidade educativa é mais do que os resultados dos testes

The number one quality business leaders look for in employees is creativity and yet the U.S. education system undermines the development of the higher-order skills that promote creativity by its dogged focus on multiple-choice tests. Stephan Turnipseed and Linda Darling- Hammond discuss the kind of rich accountability system that will help students develop into the skilled adults that modern business and industry require. La primera cualidad que los líderes de negocios de calidad buscan en sus empleados es la creatividad y sin embargo el sistema educativo de Estados Unidos socava el desarrollo de las habilidades de orden superior que promueven la creatividad por su enfoque prioritario en pruebas de selección múltiple. Stephan Turnipseed y Linda Darling-Hammond discuten un sistema de responsabilidad educativa que ayudará a los estudiantes a desarrollar como adultos las capacitades que los negocios y las industrias modernas requieren.A primeira qualidade que os líderes empresariais  procuram em seus funcionários é a criatividade, embora o sistema de ensino dos EUA prejudique o desenvolvimento de habilidades de ordem superior que promovem a criatividade porque o seu objectivo prioritário é obter bons resultados em testes de múltipla escolha. Stephan Turnipseed e Linda Darling-Hammond discutem um sistema de responsabilidade educativa que irá ajudar aos alunos a desenvolver como adultos as capacidades que as empresas e indústrias modernas exigem

Downscaling extremes: A comparison of extreme value distributions in point-source and gridded precipitation data

There is substantial empirical and climatological evidence that precipitation extremes have become more extreme during the twentieth century, and that this trend is likely to continue as global warming becomes more intense. However, understanding these issues is limited by a fundamental issue of spatial scaling: most evidence of past trends comes from rain gauge data, whereas trends into the future are produced by climate models, which rely on gridded aggregates. To study this further, we fit the Generalized Extreme Value (GEV) distribution to the right tail of the distribution of both rain gauge and gridded events. The results of this modeling exercise confirm that return values computed from rain gauge data are typically higher than those computed from gridded data; however, the size of the difference is somewhat surprising, with the rain gauge data exhibiting return values sometimes two or three times that of the gridded data. The main contribution of this paper is the development of a family of regression relationships between the two sets of return values that also take spatial variations into account. Based on these results, we now believe it is possible to project future changes in precipitation extremes at the point-location level based on results from climate models.Comment: Published in at http://dx.doi.org/10.1214/09-AOAS287 the Annals of Applied Statistics (http://www.imstat.org/aoas/) by the Institute of Mathematical Statistics (http://www.imstat.org

Forced distribution rating systems and team collaboration

This study provides three real-effort experiments on how a forced distribution rating system (FDRS) influences team collaboration. In the first and the second experiment, we examine the performance implications of an FDRS in a card sequencing task (1) when working alone and (2) when working in a team. In the third experiment, we test how an FDRS affects knowledge sharing within teams. Our findings show that an FDRS increases the speed of completing the card sequencing task when working alone and decreases the speed of completing the card sequencing task when working in a team. Beyond that, we find that an FDRS also significantly decreases knowledge sharing within teams. As the FDRS was perceived as unfair in collaborative settings but not when working alone, we provide evidence on the role of perceived justice concerning the effects of an FDRS and shed light on the psychological and economic consequences of introducing an FDRS in environments where team collaboration is essential for success. © 2021 The Author(s

Draft Genome Sequences of Pandrug-Resistant Serratia marcescens Clinical Isolates Harboring bla NDM-1

The draft genome sequences of two clonal, pandrug-resistant Serratia marcescens clinical isolates were determined. The resistance phenotype was plasmid driven, as 14 of 17 resistance genes were present on large IncFIB(K), IncHI2, and IncA/C2 plasmids indicating a large pool of transmissible antibiotic resistance genes

Reactions in the radiosensitizer misonidazole induced by low-energy (0–10 ev) electrons

PD/BD/114452/2016 UID/FIS/00068/2019 PD/00193/2012 ANR-10-LABX-0066 ANR-11-IDEX-0007Misonidazole (MISO) was considered as radiosensitizer for the treatment of hypoxic tumors. A prerequisite for entering a hypoxic cell is reduction of the drug, which may occur in the early physical-chemical stage of radiation damage. Here we study electron attachment to MISO and find that it very effectively captures low energy electrons to form the non-decomposed molecular anion. This associative attachment (AA) process is exclusively operative within a very narrow resonance right at threshold (zero electron energy). In addition, a variety of negatively charged fragments are observed in the electron energy range 0–10 eV arising from dissociative electron attachment (DEA) processes. The observed DEA reactions include single bond cleavages (formation of NO2−), multiple bond cleavages (excision of CN−) as well as complex reactions associated with rearrangement in the transitory anion and formation of new molecules (loss of a neutral H2O unit). While any of these AA and DEA processes represent a reduction of the MISO molecule, the radicals formed in the course of the DEA reactions may play an important role in the action of MISO as radiosensitizer inside the hypoxic cell. The present results may thus reveal details of the molecular description of the action of MISO in hypoxic cells.publishersversionpublishe

Resonance Raman Spectro-Electrochemistry to Illuminate Photo-Induced Molecular Reaction Pathways

Electron transfer reactions play a key role for artificial solar energy conversion, however, the underlying reaction mechanisms and the interplay with the molecular structure are still poorly understood due to the complexity of the reaction pathways and ultrafast timescales. In order to investigate such light-induced reaction pathways, a new spectroscopic tool has been applied, which combines UV-vis and resonance Raman spectroscopy at multiple excitation wavelengths with electrochemistry in a thin-layer electrochemical cell to study [RuII(tbtpy)2]2+ (tbtpy = tri-tert-butyl-2,2′:6′,2′′-terpyridine) as a model compound for the photo-activated electron donor in structurally related molecular and supramolecular assemblies. The new spectroscopic method substantiates previous suggestions regarding the reduction mechanism of this complex by localizing photo-electrons and identifying structural changes of metastable intermediates along the reaction cascade. This has been realized by monitoring selective enhancement of Raman-active vibrations associated with structural changes upon electronic absorption when tuning the excitation wavelength into new UV-vis absorption bands of intermediate structures. Additional interpretation of shifts in Raman band positions upon reduction with the help of quantum chemical calculations provides a consistent picture of the sequential reduction of the individual terpyridine ligands, i.e., the first reduction results in the monocation [(tbtpy)Ru(tbtpy•)]+, while the second reduction generates [(tbtpy•)Ru(tbtpy•)]0 of triplet multiplicity. Therefore, the combination of this versatile spectro-electrochemical tool allows us to deepen the fundamental understanding of light-induced charge transfer processes in more relevant and complex systems

Perspectives of UK Pakistani women on their behaviour change to prevent type 2 diabetes: Qualitative study using the theory domain framework

Background: Type 2 diabetes (T2D) is a debilitating disease, highly prevalent in UK South Asians, and preventable by lifestyle intervention. The ‘New life, New you' (NLNY) physical activity (PA) and dietary intervention for T2D prevention was culturally adapted to better engage minority ethnic populations and tested for feasibility. Objectives: To investigate Pakistani female participants' perspectives of their behaviour change and of salient intervention features. Setting: A community-based 8-week programme of group delivered PA sessions with behavioural counselling and dietary advice, culturally adapted for ethnic minority populations, in an area of socioeconomic deprivation. Participants to NLNY were recruited through screening events in community venues across the town. Participants: Interviews were conducted with 20 Pakistani female NLNY participants, aged 26-45 (mean 33.5) years, from different parts of town. Results: Within the a priori Theoretical Domains Framework (intentions and goals, reinforcement, knowledge, nature of the activity, social role and identity, social influences, capabilities and skills, regulation and decision, emotion and environment), we identified the importance of social factors relating to participants' own PA and dietary behaviour change. We also identified cross-cutting themes as collateral benefits of the intervention including participants' ‘psychological health'; ‘responsibility' (for others' health, especially family members included in the new PA and diet regimes) and ‘inclusion' (an ethos of accommodating differences). Conclusions:  Our findings suggest that culturally adapted interventions for Pakistani women at risk of T2D, delivered via group PA sessions with counselling and dietary advice, may encourage their PA and dietary behaviour change, and have collateral health and social benefits. The NLNY intervention appeared to be acceptable. We plan to evaluate recruitment, retention and likely effect of the intervention on participant behaviour prior to definitive evaluation

Participants' perspectives on making and maintaining behavioural changes in a lifestyle intervention for type 2 diabetes prevention: A qualitative study using the theory domain framework

Objectives: In a qualitative substudy, we sought to elicit participants' perspectives of their behavioural change and maintenance of new behaviours towards intervention optimisation. Setting: The intervention was delivered in leisure and community settings in a local authority, which according to the UK government statistics ranks as 1 of the 10 most socioeconomically deprived areas in England. Participants: We recruited 218 adults aged 40-65 years at elevated risk of type 2 diabetes (Finnish Diabetes Risk Score≥11) to the intervention. Follow-up at 12 months was completed by 134 (62%). We recruited 15 participants, purposively sampled for physical activity increase, to the qualitative substudy. Intervention: Lifestyle intervention can prevent type 2 diabetes, but translation to service provision remains challenging. The ‘New life, New you' intervention aimed to promote physical activity, healthy eating and weight loss, and included supervised group physical activity sessions. Behavioural change and weight loss at 12-month follow-up were encouraging. Design: We conducted 15 individual semistructured interviews. The Framework approach, with a comparison of emerging themes, was used in analysis of the transcribed data and complemented by the Theory Domains Framework. Results: Themes emerging from the data were grouped as perceptions that promoted initiating, enacting and maintaining behavioural change. The data were then categorised in accordance with the Theory Domains Framework: intentions and goals; reinforcement; knowledge; social role and identity; social influences; skills and beliefs about capabilities; behavioural regulation, memory, emotion, attention and decision processes and environmental context and resources. Participant perceptions of intervention features that facilitated behavioural change processes were then similarly analysed. Conclusions: Social influences, reference to social role and identity (eg, peer support), and intentions and goals (eg, to lose weight) were dominant themes across the three phases of behavioural change. Reinforcement, regulation and decision processes were more evident in the maintenance phase. The socioeconomic status of participants was reflected in the environmental context and resource theme. Analysis of phases and theoretical domains of behavioural change added depth and utility to inform intervention optimisation. We will develop the intervention with improved peer support and explicit monitoring of the behavioural change techniques used, prior to a definitive trial