Metamorphic development of the olfactory system in the red flour beetle (Tribolium castaneum, HERBST)

Background: Insects depend on their olfactory sense as a vital system. Olfactory cues are processed by a rather complex system and translated into various types of behavior. In holometabolous insects like the red flour beetle Tribolium castaneum, the nervous system typically undergoes considerable remodeling during metamorphosis. This process includes the integration of new neurons, as well as remodeling and elimination of larval neurons. Results: We find that the sensory neurons of the larval antennae are reused in the adult antennae. Further, the larval antennal lobe gets transformed into its adult version. The beetle’s larval antennal lobe is already glomerularly structured, but its glomeruli dissolve in the last larval stage. However, the axons of the olfactory sensory neurons remain within the antennal lobe volume. The glomeruli of the adult antennal lobe then form from mid-metamorphosis independently of the presence of a functional OR/Orco complex but mature dependent on the latter during a postmetamorphic phase. Conclusions: We provide insights into the metamorphic development of the red flour beetle’s olfactory system and compared it to data on Drosophila melanogaster, Manduca sexta, and Apis mellifera. The comparison revealed that some aspects, such as the formation of the antennal lobe’s adult glomeruli at mid-metamorphosis, are common, while others like the development of sensory appendages or the role of Orco seemingly differ

Organic Pig Production in Europe - Health Management in Common Organic Pig Farming

Organic farmers in Europe have developed different housing systems for pigs based on the availability of land, soil characteristics, climate, tradition and national organic certification schemes. This guide gives an overview of the typical housing systems used for pigs in organic farming. It lists advantages and disadvantages of the different systems and provides relevant recommendations to farmers for health managment

Cost comparison of insulin glargine with insulin detemir in a basal-bolus regime with mealtime insulin aspart in type 2 diabetes in Germany

Objective: To compare the treatment costs of insulin glargine (IG; Lantus®) to detemir (ID; Levemir®), both combined with bolus insulin aspart (NovoRapid®) in type 2 diabetes (T2D) in Germany

Epidemiological study concerning the characteristics of organic pig farming in selected European countries

Interviews and on-farm assessments were conducted in a total of 101 organic pig farms in different European countries (Austria, Denmark, France, Germany, Italy, and Sweden) to gain knowledge about farm management and health status of organic pigs in Europe. 66 farms kept at least one age group outdoors while 35 farms kept their pigs exclusively indoors, for the most part with a concrete outside run. Housing and feeding conditions were characterised by a large heterogeneity within and between European countries. On many farms, the feeding regimes seemed suboptimal, leaving ample room for easily feasible improvements. With respect to the health management, some farms made comprehensive use of the various options, whereas many farmers neglected the implementation of preventive measures, including appropriate hygiene and disinfection measures. On the majority of organic farms with indoor housing, the options for disinfection were hindered by the fact that many farms were not able to implement an all-in all-out concept. In correspondence with the large variation in the living conditions for pigs, also production data and mortality rates differed widely between organic pig farms. According to the estimation by the farmer concerning the occurrence of selected animal health problems, mortality of suckling piglets and weaners and weaning diarrhoea were named as the most relevant disease problems. Although dedicated to the same minimum standards, it can be concluded that organic pig farming does not provide the same living conditions or a homogenous outcome of animal health parameters but, like all systems, also depends on the quality of management. Differences in management practices, housing conditions, restrictions in the availability of resources (labour time, financial budget etc.), and a lack of feedback and control mechanism within the farm system appears to be a main reason for the substantial variation between farms

3D Standard Brain of the Red Flour Beetle Tribolium Castaneum: A Tool to Study Metamorphic Development and Adult Plasticity

The red flour beetle Tribolium castaneum is emerging as a further standard insect model beside Drosophila. Its genome is fully sequenced and it is susceptible for genetic manipulations including RNA-interference. We use this beetle to study adult brain development and plasticity primarily with respect to the olfactory system. In the current study, we provide 3D standard brain atlases of freshly eclosed adult female and male beetles (A0). The atlases include eight paired and three unpaired neuropils including antennal lobes (ALs), optic lobe neuropils, mushroom body calyces and pedunculi, and central complex. For each of the two standard brains, we averaged brain areas of 20 individual brains. Additionally, we characterized eight selected olfactory glomeruli from 10 A0 female and male beetles respectively, which we could unequivocally recognize from individual to individual owing to their size and typical position in the ALs. In summary, comparison of the averaged neuropil volumes revealed no sexual dimorphism in any of the reconstructed neuropils in A0 Tribolium brains. Both, the female and male 3D standard brain are also used for interspecies comparisons, and, importantly, will serve as future volumetric references after genetical manipulation especially regarding metamorphic development and adult plasticity

Bioschweinehaltung in Europa - Tierhaltungssysteme und Gesundheitsmanagement

Basierend auf der Betriebsfläche, den vorherrschenden Bodentypen, dem Klima, Traditionen und nationalen Gesetzgebungen für den Biolandbau sind in Europa verschiedene Haltungssysteme für Bioschweine entstanden. Dieses Merkblatt bietet eine Übersicht über die Systeme und zeigt deren Vor- und Nachteile auf. Empfehlungen für das Management in den Systemen ergänzen die Profile

Ekologisk Grisproduktion i Europa - Guide för förbättrad djurhälsa i ekologisk grisproduktion

Ekologiska lantbrukare i Europa har utvecklat olika inhysningssystem för grisar som baseras på arealtillgång, markförhållanden, klimat, tradition och nationella ekologiska certifieringsregler. Denna guide ger en överblick av de vanligaste inhysningssystemen som används för grisar i ekologiskt lantbruk i Europa. Den beskriver fördelar och nackdelar med olika typer av system och ger relevanta rekommendationer till lantbrukare för hälsoövervakning

Morphological and Transcriptomic Analysis of a Beetle Chemosensory System Reveals a Gnathal Olfactory Center

OR gene tissue expression and their chromosomal localization. a Venn diagram showing the number of ORs expressed (RPKM ≥ 0.5) in the different body parts: antennae, legs, mouthparts (as piece of the head capsule anterior of the antennae), heads (the whole head capsule including mouthparts but excluding the antennae), and bodies (excluding head and legs). b Venn diagram comparing our results (yellow, green) with data from Engsontia et al. [115] (blue, red). Number of expressed ORs, defined by RPKM ≥ 0.5 (yellow), by RT-PCR (blue), not expressed RPKM < 0.5 (green), or with no RT-PCR amplicon (red). ORs of the brown group were not previously tested by Engsontia et al. c Chromosomal localization of T. castaneum ORs. Based on the Georgia GA-2 strain genome assembly 3.0 [81], only chromosomal linkage groups containing an IR or SNMP are depicted. Gene clusters are indicated by a number referring to the chromosome and a letter conveys the relative position on the chromosome. The number of genes within this cluster is indicated in the square brackets. (PDF 277 kb

Integrative Sustainability Analysis of European Pig Farms: Development of a Multi-Criteria Assessment Tool

Societal interest in all aspects of sustainability has increased. Therefore, pig farmers need to be aware of their strengths and weaknesses in all dimensions of sustainability: economy, environment, social wellbeing, and animal health and welfare. Our aim was to describe and critically discuss the development of a sustainability assessment tool for pig farms and to evaluate its suitability by applying it to 63 European pig farms (13 breeding, 27 breeding-to-finishing, and 23 finishing farms). The multi-criteria assessment tool was developed in several steps (the selection and scaling of indicators and their aggregation and weighting) in order to summarise the indicators into subtheme and theme scores. The indicators contributing the most to the subtheme/theme scores were identified and discussed in order to evaluate the procedure of the development. For example, some indicators, such as Ecological compensation area, Fairness of prices, and Tail docking, for which farms were scored low, were also identified as “real world problems” in other studies. For other sustainability aspects with low performance, the threshold might have been set too ambitiously, e.g., for Number of sows per annual working unit. Furthermore, to analyse the suitability of the tool, we assessed the best and worst median theme scores (good and poor performances) for each dimension, as well as the variability of the performances of the farms within the themes. Some themes were found to be moderate, such as Pig comfort, Biodiversity, or Resilience, whereas others were found to be good, e.g., Water and the Human–animal relationship, as well as several themes of the social wellbeing dimension. Overall, the sustainability tool provides a comprehensive assessment of the sustainability of pig production. Furthermore, this publication contributes to both the theory (development of a robust sustainability tool) and the practice (provision of a tool to assess and benchmark the sustainability on farms). As a next step, a sensitivity analysis should be performed, and the tool should be applied for further development