High resolution imaging at large telescopes

Image recovery at a resolution limited only by diffraction is now possible at large telescopes. The theory of speckle image reconstruction is explained and the current status of a video recording and digitization system for the reconstruction procedure is described. Potential applications of the process when used with very large telescopes are discussed. The constraints on telescope design imposed by these techniques are listed

A joint Monte Carlo analysis of seafloor compliance, Rayleigh wave dispersion and receiver functions at ocean bottom seismic stations offshore New Zealand

Author Posting. © American Geophysical Union, 2014. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Geochemistry, Geophysics, Geosystems 15 (2014): 5051–5068, doi:10.1002/2014GC005412.Teleseismic body-wave imaging techniques such as receiver function analysis can be notoriously difficult to employ on ocean-bottom seismic data due largely to multiple reverberations within the water and low-velocity sediments. In lieu of suppressing this coherently scattered noise in ocean-bottom receiver functions, these site effects can be modeled in conjunction with shear velocity information from seafloor compliance and surface wave dispersion measurements to discern crustal structure. A novel technique to estimate 1-D crustal shear-velocity profiles from these data using Monte Carlo sampling is presented here. We find that seafloor compliance inversions and P-S conversions observed in the receiver functions provide complimentary constraints on sediment velocity and thickness. Incoherent noise in receiver functions from the MOANA ocean bottom seismic experiment limit the accuracy of the practical analysis at crustal scales, but synthetic recovery tests and comparison with independent unconstrained nonlinear optimization results affirm the utility of this technique in principle.This research was supported by the National Science Foundation under grants EAR-0409835 and EAR-0409609. J.C.S. was supported by a CIRES visiting fellowship at the University of Colorado and A.F.S. was supported by a visiting professorship at the Earthquake Research Institute, University of Tokyo, for a portion of this work.2015-06-1

Lithospheric shear velocity structure of South Island, New Zealand, from amphibious Rayleigh wave tomography

Author Posting. © American Geophysical Union, 2016. This article is posted here by permission of American Geophysical Union for personal use, not for redistribution. The definitive version was published in Journal of Geophysical Research: Solid Earth 121 (2016): 3686–3702, doi:10.1002/2015JB012726.We present a crust and mantle 3-D shear velocity model extending well offshore of New Zealand's South Island, imaging the lithosphere beneath the South Island as well as the Campbell and Challenger Plateaus. Our model is constructed via linearized inversion of both teleseismic (18–70 s period) and ambient noise-based (8–25 s period) Rayleigh wave dispersion measurements. We augment an array of 4 land-based and 29 ocean bottom instruments deployed off the South Island's east and west coasts in 2009–2010 by the Marine Observations of Anisotropy Near Aotearoa experiment with 28 land-based seismometers from New Zealand's permanent GeoNet array. Major features of our shear wave velocity (Vs) model include a low-velocity (Vs 50 km) beneath the central South Island exhibits strong spatial correlation with upper mantle earthquake hypocenters beneath the Alpine Fault. The ~400 km long low-velocity zone we image beneath eastern South Island and the inner Bounty Trough underlies Cenozoic volcanics and the locations of mantle-derived helium measurements, consistent with asthenospheric upwelling in the region.National Science Foundation Grant Number: EAR-0409564, EAR-0409609, and EAR-04098352016-11-2

Ozone Production and Loss Rate Measurements in the Middle Stratosphere

The first simultaneous measurements of HO(x), NO(x), and Cl(x) radicals in the middle stratosphere show that NO(x) catalytic cycles dominate loss of ozone (O3) for altitudes between 24 and 38 km; Cl(x) catalytic cycles are measured to be less effective than previously expected; and there is no 'ozone deficit' in the photochemically dominated altitude range from 31 and 38 km, contrary to some previous theoretical studies

Type I interferon responses of common carp strains with different levels of resistance to koi herpesvirus disease during infection with CyHV-3 or SVCV

Carp from breeding strains with different genetic background present diverse levels of resistance to viral pathogens. Carp strains of Asian origin, currently being treated as Cyprinus rubrofuscus L., especially Amur wild carp (AS), were proven to be more resistant to koi herpesvirus disease (KHVD; caused by cyprinid herpesvirus 3, CyHV-3) than strains originating from Europe and belonging to Cyprinus carpio L., like the Prerov scale carp (PS) or koi carp from a breed in the Czech Republic. We hypothesised that it can be associated with a higher magnitude of type I interferon (IFN) response as a first line of innate defence mechanisms against viral infections. To evaluate this hypothesis, four strains of common carp (AS, Rop, PS and koi) were challenged using two viral infection models: Rhabdovirus SVCV (spring viremia of carp virus) and alloherpesvirus CyHV-3. The infection with SVCV induced a low mortality rate and the most resistant was the Rop strain (no mortalities), whereas the PS strain was the most susceptible (survival rate of 78%). During CyHV-3 infection, Rop and AS strains performed better (survival rates of 78% and 53%, respectively) than PS and koi strains (survival rates of 35% and 10%, respectively). The evaluation of virus loads and virus replication showed significant differences between the carp strains, which correlated with the mortality rate. The evaluation of type I IFN responses showed that there were fundamental differences between the virus infection models. While responses to the SVCV were high, the CyHV-3 generally induced low responses. Furthermore, the results demonstrated that the magnitude of type I IFN responses did not correlate with a higher resistance in infected carp. In the case of a CyHV-3 infection, reduced type I IFN responses could be related to the potential ability of the virus to interfere with cellular sensing of foreign nucleic acids. Taken together, the results broaden our understanding of how common carp from different genetic lines interact with various viral pathogens

Validation of the Aura Microwave Limb Sounder HNOmeasurements

We assess the quality of the version 2.2 (v2.2) HNO3 measurements from the Microwave Limb Sounder (MLS) on the Earth Observing System Aura satellite. The MLS HNO3 product has been greatly improved over that in the previous version (v1.5), with smoother profiles, much more realistic behavior at the lowest retrieval levels, and correction of a high bias caused by an error in one of the spectroscopy files used in v1.5 processing. The v2.2 HNO3 data are scientifically useful over the range 215 to 3.2 hPa, with single-profile precision of ∼0.7 ppbv throughout. Vertical resolution is 3–4 km in the upper troposphere and lower stratosphere, degrading to ∼5 km in the middle and upper stratosphere. The impact of various sources of systematic uncertainty has been quantified through a comprehensive set of retrieval simulations. In aggregate, systematic uncertainties are estimated to induce in the v2.2 HNO3 measurements biases that vary with altitude between ±0.5 and ±2 ppbv and multiplicative errors of ±5–15% throughout the stratosphere, rising to ∼±30% at 215 hPa. Consistent with this uncertainty analysis, comparisons with correlative data sets show that relative to HNO3 measurements from ground-based, balloon-borne, and satellite instruments operating in both the infrared and microwave regions of the spectrum, MLS v2.2 HNO3 mixing ratios are uniformly low by 10–30% throughout most of the stratosphere. Comparisons with in situ measurements made from the DC-8 and WB-57 aircraft in the upper troposphere and lowermost stratosphere indicate that the MLS HNO3 values are low in this region as well, but are useful for scientific studies (with appropriate averaging)

Greenland tidewater glacier advanced rapidly during era of Norse Settlement

ACKNOWLEDGMENTS We thank the Greenland Institute of Natural Resources for providing logistical support in Nuuk. Martin Blicher, Thomas Juul-Pedersen, and Johanne Vad are thanked for their research and field assistance. We acknowledge the support of the National Museum of Greenland for permission to undertake excavations near Norse ruin sites (permit 2015/03). Project funding was provided by the Leverhulme Trust Research Project grant 2014-093, and J.M. Lea was supported by funding from the Quaternary Research Association, British Society for Geomorphology, and a UK Research and Innovation (UKRI) Future Leaders Fellowship (MR/S017232/1). We thank two anonymous reviewers and the editor for constructive comments, which helped to substantially improve this paper. D.M. Pearce would like to dedicate this paper to her father Richard M. Pearce.Peer reviewedPostprin

Multimodal X-ray imaging of nanocontainer-treated macrophages and calcium distribution in the perilacunar bone matrix

Studies of biological systems typically require the application of several complementary methods able to yield statistically-relevant results at a unique level of sensitivity. Combined X-ray fluorescence and ptychography offer excellent elemental and structural imaging contrasts at the nanoscale. They enable a robust correlation of elemental distributions with respect to the cellular morphology. Here we extend the applicability of the two modalities to higher X-ray excitation energies, permitting iron mapping. Using a long-range scanning setup, we applied the method to two vital biomedical cases. We quantified the iron distributions in a population of macrophages treated with Mycobacterium-tuberculosis-targeting iron-oxide nanocontainers. Our work allowed to visualize the internalization of the nanocontainer agglomerates in the cytosol. From the iron areal mass maps, we obtained a distribution of antibiotic load per agglomerate and an average areal concentration of nanocontainers in the agglomerates. In the second application we mapped the calcium content in a human bone matrix in close proximity to osteocyte lacunae (perilacunar matrix). A concurrently acquired ptychographic image was used to remove the mass-thickness effect from the raw calcium map. The resulting ptychography-enhanced calcium distribution allowed then to observe a locally lower degree of mineralization of the perilacunar matrix

The history and evolution of the clinical effectiveness of haemophilia type a treatment: a systematic review.

First evidence of cases of haemophilia dates from ancient Egypt, but it was when Queen Victoria from England in the 19th century transmitted this illness to her descendants, when it became known as the "royal disease". Last decades of the 20th century account for major discoveries that improved the life expectancy and quality of life of these patients. The history and evolution of haemophilia healthcare counts ups and downs. The introduction of prophylactic schemes during the 1970s have proved to be more effective that the classic on-demand replacement of clotting factors, nevertheless many patients managed with frequent plasma transfusions or derived products became infected with the Human Immunodeficiency Virus (HIV) and Hepatitis C virus during the 1980s and 1990s. Recombinant factor VIII inception has decreased the risk of blood borne infections and restored back longer life expectancies. Main concerns for haemophilia healthcare are shifting from the pure clinical aspects to the economic considerations of long-term replacement therapy. Nowadays researchers' attention has been placed on the future costs and cost-effectiveness of costly long-term treatment. Equity considerations are relevant as well, and alternative options for less affluent countries are under the scope of further research. The aim of this review was to assess the evidence of different treatment options for haemophilia type A over the past four decades, focusing on the most important technological advances that have influenced the natural course of this "royal disease"

High-Altitude MMIC Sounding Radiometer for the Global Hawk Unmanned Aerial Vehicle

Microwave imaging radiometers operating in the 50-183 GHz range for retrieving atmospheric temperature and water vapor profiles from airborne platforms have been limited in the spatial scales of atmospheric structures that are resolved not because of antenna aperture size, but because of high receiver noise masking the small variations that occur on small spatial scales. Atmospheric variability on short spatial and temporal scales (second/ km scale) is completely unresolved by existing microwave profilers. The solution was to integrate JPL-designed, high-frequency, low-noise-amplifier (LNA) technology into the High-Altitude MMIC Sounding Radiometer (HAMSR), which is an airborne microwave sounding radiometer, to lower the system noise by an order of magnitude to enable the instrument to resolve atmospheric variability on small spatial and temporal scales. HAMSR has eight sounding channels near the 60-GHz oxygen line complex, ten channels near the 118.75-GHz oxygen line, and seven channels near the 183.31-GHz water vapor line. The HAMSR receiver system consists of three heterodyne spectrometers covering the three bands. The antenna system consists of two back-to-back reflectors that rotate together at a programmable scan rate via a stepper motor. A single full rotation includes the swath below the aircraft followed by observations of ambient (roughly 0 C in flight) and heated (70 C) blackbody calibration targets located at the top of the rotation. A field-programmable gate array (FPGA) is used to read the digitized radiometer counts and receive the reflector position from the scan motor encoder, which are then sent to a microprocessor and packed into data files. The microprocessor additionally reads telemetry data from 40 onboard housekeeping channels (containing instrument temperatures), and receives packets from an onboard navigation unit, which provides GPS time and position as well as independent attitude information (e.g., heading, roll, pitch, and yaw). The raw data files are accessed through an Ethernet port. The HAMSR data rate is relatively low at 75 kbps, allowing for real-time access over the Global Hawk high-data-rate downlink. Once on the ground, the raw data are unpacked and processed through two levels of processing. The Level 1 product contains geo-located, time-stamped, calibrated brightness temperatures for the Earth scan. These data are then input to a lD variational retrieval algorithm to produce temperature, water vapor, and cloud liquid water profiles, as well as several derived products such as potential temperature and relative humidity