Blood and alveolar lymphocyte subsets in pulmonary cytomegalovirus infection after lung transplantation

BACKGROUND: Cytomegalovirus (CMV) pneumonitis has been shown to be associated with lymphocytic alveolitis after lung transplantation. In the present study, we investigated a series of bronchoalveolar (BAL) and blood samples, collected in the absence of rejection or acute infectious episodes. in order -1: to evaluate intra-alveolar cell population changes concomitant with CMV replication and -2: to reappraise the value of cell population analysis in the management of patients after lung transplantation. METHODS: We used flow cytometry to investigate modifications of lymphocyte subpopulations related to pulmonary cytomegalovirus infections in blood and BAL samples from a series of 13 lung transplant recipients. After exclusion of samples obtained during pulmonary rejection, bronchiolitis obliterans or acute bacterial infection, 48 blood and BAL samples were retained for analysis: 17 were CMV positive by shell-vial assay and 31 were CMV negative in blood and BAL. RESULTS: Our results demonstrate that pulmonary CMV infection is associated with a significant increase in the total lymphocyte population in BAL samples, but with minor modifications of the various lymphocyte subpopulations and a significantly higher absolute number of B lymphocytes in blood samples. CONCLUSIONS: Cytomegalovirus pulmonary infection is accompanied by only minor changes in BAL lymphocyte subpopulations. The study of BAL lymphocyte subpopulations therefore appears to be of limited clinical value in the diagnosis of pulmonary CMV infection. However, increased blood B-lymphocytes seems to be a clinical feature associated with CMV infection

A simple mechanism for the reversals of Earth's magnetic field

We show that a model, recently used to describe all the dynamical regimes of the magnetic field generated by the dynamo effect in the VKS experiment [1], also provides a simple explanation of the reversals of Earth's magnetic field, despite strong differences between both systems.Comment: update version, with new figure

Nonlinear magnetic induction by helical motion in a liquid sodium turbulent flow

International audienceWe report an experimental study of the magnetic field (B) over right arrow induced by a turbulent swirling flow of liquid sodium submitted to a transverse magnetic field (B) over right arrow (0). We show that the induced field can behave nonlinearly as a function of the magnetic Reynolds number, R-m. At low R-m, the induced mean field along the axis of the flow, , and the one parallel to (B) over right arrow (0), , first behave like R-m(2), whereas the third component, , is linear in R-m. The sign of is determined by the flow helicity. At higher R-m, (B) over right arrow strongly depends on the local geometry of the mean flow: decreases to zero in the core of the swirling flow but remains finite outside. We compare the experimental results with the computed magnetic induction due to the mean flow alone

Experimental Observation of Spatially Localized Dynamo Magnetic Fields

International audienceWe report the first experimental observation of a spatially localized dynamo magnetic field, a common feature of astrophysical dynamos and convective dynamo simulations. When the two propellers of the von Ka ́rma ́n sodium experiment are driven at frequencies that differ by 15%, the mean magnetic field's energy measured close to the slower disk is nearly 10 times larger than the one close to the faster one. This strong localization of the magnetic field when a symmetry of the forcing is broken is in good agreement with a prediction based on the interaction between a dipolar and a quadrupolar magnetic mode

Magnetohydrodynamics measurements in the von Karman sodium experiment

International audienceWe study the magnetic induction in a confined swirling flow of liquid sodium, at integral magnetic Reynolds numbers up to 50. More precisely, we measure in situ the magnetic field induced by the flow motion in the presence of a weak external field. Because of the very small value of the magnetic Prandtl number of all liquid metals, flows with even modest R-m are strongly turbulent. Large mean induction effects are observed over a fluctuating background. As expected from the von Karman flow geometry, the induction is strongly anisotropic. The main contributions are the generation of an azimuthal induced field when the applied field is in the axial direction (an Omega effect) and the generation of axial induced field when the applied field is the transverse direction (as in a large scale alpha effect). Strong fluctuations of the induced field, due to the flow nonstationarity, occur over time scales slower than the flow forcing frequency. In the spectral domain, they display a f(-1) spectral slope. At smaller scales (and larger frequencies) the turbulent fluctuations are in agreement with a Kolmogorov modeling of passive vector dynamics

The von Karman Sodium experiment: Turbulent dynamical dynamos

International audienceThe von Karman Sodium (VKS) experiment studies dynamo action in the flow generated inside a cylinder filled with liquid sodium by the rotation of coaxial impellers (the von Karman geometry). We first report observations related to the self-generation of a stationary dynamo when the flow forcing is R-pi-symmetric, i.e., when the impellers rotate in opposite directions at equal angular velocities. The bifurcation is found to be supercritical with a neutral mode whose geometry is predominantly axisymmetric. We then report the different dynamical dynamo regimes observed when the flow forcing is not symmetric, including magnetic field reversals. We finally show that these dynamics display characteristic features of low dimensional dynamical systems despite the high degree of turbulence in the flow

Chaotic dynamos generated by a turbulent flow of liquid sodium

International audienceWe report the observation of several dynamical regimes of the magnetic field generated by a turbulent flow of liquid sodium (VKS experiment). Stationary dynamos, transitions to relaxation cycles or to intermittent bursts, and random field reversals occur in a fairly small range of parameters. Large scale dynamics of the magnetic field result from the interactions of a few modes. The low dimensional nature of these dynamics is not smeared out by the very strong turbulent fluctuations of the flow

Mutations in STAT3 and IL12RB1 impair the development of human IL-17–producing T cells

The cytokines controlling the development of human interleukin (IL) 17–producing T helper cells in vitro have been difficult to identify. We addressed the question of the development of human IL-17–producing T helper cells in vivo by quantifying the production and secretion of IL-17 by fresh T cells ex vivo, and by T cell blasts expanded in vitro from patients with particular genetic traits affecting transforming growth factor (TGF) β, IL-1, IL-6, or IL-23 responses. Activating mutations in TGFB1, TGFBR1, and TGFBR2 (Camurati-Engelmann disease and Marfan-like syndromes) and loss-of-function mutations in IRAK4 and MYD88 (Mendelian predisposition to pyogenic bacterial infections) had no detectable impact. In contrast, dominant-negative mutations in STAT3 (autosomal-dominant hyperimmunoglobulin E syndrome) and, to a lesser extent, null mutations in IL12B and IL12RB1 (Mendelian susceptibility to mycobacterial diseases) impaired the development of IL-17–producing T cells. These data suggest that IL-12Rβ1– and STAT-3–dependent signals play a key role in the differentiation and/or expansion of human IL-17–producing T cell populations in vivo