Additional file 1 of Rab37 mediates trafficking and membrane presentation of PD-1 to sustain T cell exhaustion in lung cancer

Additional file 1: Table S1. The plasmids and their characteristics used in the current study. Table S2. Antibodies and their reaction conditions used in the current study. Table S3. Characteristics of NSCLC patients and normal individuals for ex vivo assays in the current study. Figure S1. The expression of PD-1 and Rab37 exhibited positive correlation in T cells. Figure S2. Rab37 mediates PD-1 membrane trafficking. Figure S3. Glycosylation on PD-1 promotes protein stability and transport to the PM. Figure S4. Rab37-mediated PD-1 PM presentation reduces T cell function. Figure S5. The serum biochemical parameters and major organ histology in LLC tumor-bearing Rab37 KO and WT mice. Figure S6. The relationship between Rab37 expression and population of PD-1/TIM3/CD8 cells derived from PBMCs treated with CD3 and CD28 antibodies

Hall-Effect Sensors for Real-Time Monitoring Pier Scour

Scour around bridge pier is a major cause of bridge failure such as collapse resulted in loss of life and property. Most of available sensors and approaches for monitoring bridge pier scour are very expensive, which is a main challenge for mass deployment of numerous bridges. Our proposed scour monitoring system utilized low-cost commercial sensors, hall-effect sensors (unit price< $1) that are capable of real-time measuring bridge pier scour with resolution of ~ 2.5 cm, and overall cost for single sensor node of our proposed work is at least 40 % less expensive than existing work. The hall- effect sensor is evaluated under controlled conditions in two laboratory flumes. After scour event, the typical voltage change of the hall-effect sensor is ~ 300 mV, and the system achieve signal-to-noise ratio performance of ~ 60 dB. Finally, we also provide an equation to predict the time variation of scour depth around pier model. Moreover, the master-slave architecture of bridge pier scour monitoring system has scalability and flexibility for mass deployment. This technique has the potential for further widespread implementation in the field