STUDY OF SOME CALCIUM CHANNEL INHIBITING SPIDER TOXINS THROUGH BIOINFORMATIC TOOLS

ABSTRACT In silico comparative study of sixteen selected calcium channel inhibiting spider toxins, were made through different bioinformatics tools. Amino acid composition is predicted through Expasy's Protparam. Secondary structure analysis were done through Expasy's GOR. Hydropathy was predicted through Expasy's Protscale. TMHMM 2.0 was used for predicting disulfide bonds. Signal peptide prediction was done by Signal P 4.1 server. Cysteine disulfide bond prediction was made by DiANNA 1.1 web server. Prdos software was used for predicting protein disordered region. GenomeNet was applied for motif prediction. Phylogenetic tree was constructed by Clustal W. POPI 2.0 was used for immunogenicity prediction of these spider toxins. Results elaborated a total predictive in silico picture of various properties of these sixteen spider toxins in a comparative fashion including its immunogenicity, disorder level and evolutionary and phylogenic relationships. Hence such comparative, in silico visualization of these spider toxins can be effective in future for in vitro research on these toxins and also properties of calcium channel proteins

Universal ideals in local realities: online viewing in South Korea, Brazil and India

The potential of the internet to act as a global distribution outlet for screen content has long come into conflict with the nationally-focused strategies of producers, broadcasters, governments and internet service providers. Online viewing therefore acts as a useful case study for interrogating how tensions between ‘global’ and ‘local’ manifest within an increasingly digitized media landscape. This article examines the online viewing markets in three countries at different stages of digital maturity (South Korea, Brazil, India) to consider how online viewing has evolved in each. It then examines audience questionnaire and interview data generated in each country to explore how viewers are making sense of and valuing online viewing services. By interrogating all three samples before focusing specifically on India in more detail, it examines two tensions within the global expansion of online film and television distribution: between global trends and local infrastructures, and between the ideals of online viewing services and the grounded realities of their daily use

Design of nickel chelates of tetradentate N-heterocyclic carbenes with subdued cytotoxicity

A series of nickel complexes, 1b-3b, exhibiting subdued cytotoxicity have been designed with the intent of their use as agents for developing resistance to nickel toxicity. Indeed, the nickel complexes, 1b-3b, display less cytotoxic activity towards two commonly occurring human cancer cell lines namely, HeLa cells (16-64%) and MCF-7 cells (70-90%) in culture as compared to the maximum inhibition by NiCl2·6H2O under analogous conditions at three different concentrations (1μM, 5μM and 20μM). Similarly, the suppression of cytotoxicity through chelation of the metal ion can also be seen in normal cells as was evident from a significant reduction in cytotoxicity (9-41%) for a non-tumorigenic CHO cell line in case of a representative complex 3b. The reduction in carcinogenic activity in the complexes relative to nickel(II) ion from NiCl2·6H2O is brought about by successful chelation of the metal center by a class of specially designed new tetradentate N/O-functionalized N-heterocyclic carbene ligands. The two strongly σ-donating carbene moieties coupled with two negatively charged amido moieties present in the N-heterocyclic carbene ligands facilitate complete chelation of the metal center and thereby significantly reduce the cytotoxic effects of the metal

Abstracts of National Conference on Research and Developments in Material Processing, Modelling and Characterization 2020

This book presents the abstracts of the papers presented to the Online National Conference on Research and Developments in Material Processing, Modelling and Characterization 2020 (RDMPMC-2020) held on 26th and 27th August 2020 organized by the Department of Metallurgical and Materials Science in Association with the Department of Production and Industrial Engineering, National Institute of Technology Jamshedpur, Jharkhand, India. Conference Title: National Conference on Research and Developments in Material Processing, Modelling and Characterization 2020Conference Acronym: RDMPMC-2020Conference Date: 26–27 August 2020Conference Location: Online (Virtual Mode)Conference Organizer: Department of Metallurgical and Materials Engineering, National Institute of Technology JamshedpurCo-organizer: Department of Production and Industrial Engineering, National Institute of Technology Jamshedpur, Jharkhand, IndiaConference Sponsor: TEQIP-