47 research outputs found

    Final Evaluation of the Better Opportunities Through Self-Sufficiency Program.

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    Supported by the Minnesota Center for Survey Research, Center for Urban and Regional Affairs, and the Hubert Humphrey Institute of Public Affairs, University of Minnesota. Funds provided by the Dayton Hudson Foundation and Target Stores.Beech, Patricia; Sporlein, Barbara; Goetz, Edward G.. (1990). Final Evaluation of the Better Opportunities Through Self-Sufficiency Program.. Retrieved from the University Digital Conservancy, https://hdl.handle.net/11299/208216

    Housing Needs and Markets in Rochester and Olmsted County. Summary of a Report to the Rochester/Olmsted Community Housing Partnership, Inc.

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    A 1990 study of housing in Olmsted County found that families in lower and very low income categories as well as special populations are having problems obtaining suitable housing in this wealthy part of the state. A CURA Reporter article in April 1990 also summarized the study.A cooperative venture between the authors and the Rochester Department of Planning and Housing, with the support of the Center for Urban and Regional Affairs, University of Minnesota. Funded by the Rochester Area Foundation

    Full Report to the Rochester/Olmsted Community Housing Partnership, Inc. on Housing Needs and Markets.

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    A cooperative venture between the authors and the Rochester Department of Planning and Housing, with the support of the Center for Urban and Regional Affairs, University of Minnesota. Funded by the Rochester Area Foundation

    Ultrafast photoinduced electron transfer in coumarin 343 sensitized TiO2-colloidal solution

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    Photoinduced electron transfer from organic dye molecules to semiconductor nanoparticles is the first and most important reaction step for the mechanism in the so called “wet solar cells” [1]. The time scale between the photoexcitation of the dye and the electron injection into the conduction band of the semiconductor colloid varies from a few tens of femtoseconds to nanoseconds, depending on the specific electron transfer parameters of the system, e.g., electronic coupling or free energy values of donor and acceptor molecules [2–10]. We show that visible pump/ white light probe is a very efficient tool to investigate the electron injection reaction allowing to observe simultaneously the relaxation of the excited dye, the injection process of the electron, the cooling of the injected electron and the charge recombination reaction

    PEG- and electroporation-induced transformation in Nicotiana tabacum: influence of genotype on transformation frequencies

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    Experimental parameters for direct gene transfer with recombinant DNA encoding neomycin phosphotransferase II (NPTII) under control of eukaryotic expression signals were established. The introduced gene was shown by the growth of transformants on media containing kanamycin, by genomic blotting and by assaying NPTII activity. Leaf protoplasts from three green genotypes of varieties xanthii and petit havanna, and from four plastome-encoded albino genotypes of Nicotiana tabacum were analyzed with respect to cell division kinetics and yield of kanamycin-tolerant colonies after direct gene transfer. No clear correlation was found between the time of onset of cell division and transformation frequency

    Kinetic response of a photoperturbed allosteric protein

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    By covalently linking an azobenzene photoswitch across the binding groove of a PDZ domain, a conformational transition, similar to the one occurring upon ligand binding to the unmodified domain, can be initiated on a picosecond timescale by a laser pulse. The protein structures have been characterized in the two photoswitch states through NMR spectroscopy and the transition between them through ultrafast IR spectroscopy and molecular dynamics simulations. The binding groove opens on a 100-ns timescale in a highly nonexponential manner, and the molecular dynamics simulations suggest that the process is governed by the rearrangement of the water network on the protein surface. We propose this rearrangement of the water network to be another possible mechanism of allostery

    Structural, Thermodynamic, and Kinetic Properties of Gramicidin Analogue GS6 Studied by Molecular Dynamics Simulations and Statistical Mechanics

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    Gramicidin S (GS) analogues belong to an important class of cyclic peptides, characterized by an antiparallel double-stranded beta-sheet structure with Type II' beta-turns. Such compounds can be used as model systems to understand the folding/unfolding process of beta-hairpins and more in general of beta-structures. In the present study, we specifically investigate the folding/unfolding behavior of the hexameric Gramicidin S analogue GS6 by using all-atoms molecular dynamics (MD) simulations at different temperatures, coupled to a statistical mechanical model based on the Quasi Gaussian Entropy theory. Such an approach permits to describe the structural, thermodynamic, and kinetic properties of the peptide and to quantitatively characterize its folding/unfolding transitions. (c) 2009 Wiley Periodicals, Inc. Biopolymers 91: 1154-1160, 2009
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