Recalibration of the Tethyan shallow-benthic zonation across the Paleocene-Eocene boundary : the Egyptian record

The Galala Mountains in Egypt provide an excellent platform-basin transect with deposits spanning the Paleocene/ Eocene (P/E) boundary. These interfingering deposits enable a recalibration between platform and open marine biostratigraphic schemes. We investigated 18 sections from a shallow-water carbonate platform margin, dominated by larger benthic foraminifera, to basinal marls with pelagic and deep marine biota. The Late Paleocene to Early Eocene development of larger foraminifera is well recorded in the Galala transect, in particular the Tethyan evolutionary event known as the larger foraminifera turnover (LFT). This turnover distinguishes Paleocene assemblages dominated by glomalveolinids, miscellanids and ranikothalids typical for shallow benthic Zone 4 (SBZ4) from those of SBZ5, dominated by alveolinids, nummulitids, and orbitolitids. Our data agree with previous studies that suggested that the larger foraminifera turnover (LFT) coincides with the Paleocene/ Eocene boundary, delineated by the carbon isotopic excursion (CIE) and that it correlates with the boundary between calcareous nannofossils subzones NP9a/b, the benthic extinction event in smaller benthic foraminifera and the boundary between planktic foraminifera Biozones P5/E1

Late Maastrichtian-Early Paleocene sea level and climate changes in the Antioch Church Core (Alabama, Gulf of Mexico margin, USA): a multi-proxy approach

The Antioch Church core from central Alabama, spanning the Cretaceous-Paleogene (K-P) boundary, was investigated by a multi-proxy approach to study paleoenvironmental and sea level changes within the wellconstrained sequence stratigraphic setting of the Gulf of Mexico margin. The Antioch Church core comprises the Maastrichtian calcareous nannoplankton Zone CC25 and the Danian Zones NP1 to NP4 corresponding to the Maastrichtian planktonic foraminifera Zones CF3 and the Danian Zones P1a to P2. Facies shifts from a Maastrichtian siliciclastic to a mixed siliciclastic-carbonate depositional system during the late Danian. Sedimentary proxies indicate that depositional settings changed between littoral (foreshore) and inner and middle neritic (offshore transition zone). Four sedimentary sequences, each encompassing LST, TST, and HST were identified. Estimated water depths by using benthic foraminiferal assemblages were not exceeding 20-40 m for the Maastrichtian and 0-40 m for the Danian sequences. The succession of facies shifts within systems tracts can be very well disentangled by major and trace element data as well as by various element ratios including Zr/Rb, (Zr+Rb)/Ca, and Sr/Ca. By applying element stratigraphy, the ambiguities of the natural gamma ray log -with peaks associated either with maximum flooding surfaces or with silty lag deposits ("placer silts") during the late regressive HST- are resolved. In addition, the Zr/Rb ratio provides a good proxy for monitoring grain size distribution and sorting effects. According to the Antioch Church core data, the K-P boundary is associated with a sandstone event bed that includes ejecta spherules from the Chicxulub impact. However, the genesis of the K-P event bed, whether lowstand, tempestite- or tsunami-related, cannot be resolved from this core. In terms of clay mineralogy, the studied interval is characterized by a steady increase in smectite that parallels a decrease in kaolinite with the latter disappearing about two My after the K-P boundary during Biozone NP2. This change in the clay mineral assemblage, which is almost independent of lithology, may suggest a long-term shift from stable, tropical warm and humid climates during the latest Maastrichtian to warm climate with alternating humid and arid seasons in the middle Danian

The evolution of late Paleocene-early Eocene carbonate platforms of the Tethys

Abstrac

The evaluation of Computed Tomography hard- and software tools for micropaleontologic studies on foraminifera

Foraminifera (Forams) are single-celled amoeba-like organisms in the sea, which build a tiny calcareous multi-chambered shell for protection. Their enormous abundance, great variation of shape through time and their presence in all marine deposits made these tiny microfossils the oil companies’ best friend by facilitating the detection of new oil wells. Besides the success of forams in the oil and gas industry, they are also a most powerful tool for reconstructing climate change in the past. The shell of a foraminifer is a tiny gold mine of information both geometrical as chemical. However, until recently the best information on this architecture was only obtained through imaging the outside of a shell with Scanning Electron Microscopy (SEM), giving no clues towards internal structures other than single snapshots through breaking a specimen apart. With X-ray computed tomography (CT) it is possible to overcome this problem and uncover a huge amount of geometrical information without destructing the samples. Using the last generation of micro-CT’s, called nano-CT, because of the sub-micron resolution, it is now possible to perform adequate imaging even on these tiny samples without needing huge facilities. In this research, a comparison is made between different X-ray sources and X-ray detectors and the resulting image resolution. Both sharpness, noise and contrast are very important parameters that will have important effects on the accuracy of the results and on the speed of data-processing. Combining this tomography technique with specific image processing software, called segmentation, it is possible to obtain a 3D virtual representation of the entire forams shell. This 3D virtual object can then be used for many purposes, from which automatic measurement of the chambers size is one of the most important ones. The segmentation process is a combination of several algorithms that are often used in CT evaluation, in this work an evaluation of those algorithms is presented. Difficulties arising when the forams shell is filled with material but it still remains possible to perform adequate segmentation. The void inside the shell corresponds to the chambers of the foram and the inter-chamber connections. Using automatic separation algorithms it is possible to obtain the shape of individual chambers. The results from the segmentation process can then be used to perform a multitude of analysis on each foram. Out of the shells geometry one can derive variations in shell thickness, shell density and shell porosity. Since the geometry of each individual chamber can be derived, it is possible to track chamber size variation for one foram or between two different forams, the difference in orientation and distance between the chambers. In this work the algorithms and procedures have been applied on two forams: A. Pseudouvigerina sp., a benthic foram that lived within the sediments at the seafloor. It dates from the earliest Paleocene, 65 Ma and was collected near Brazos River, Texas. B. Globigerinoides, a modern planktic foram, living in the upper part of the water comlumn in the open ocean. The test settled on the seafloor after death and was recently collected from the seafloor at 2900 m water depth at Nazca Ridge in the eastern Pacific Ocean. It was found that foram A consists of 15 chambers with a total volume of 1.8 x 106 µm³ and shows progressive growth of consecutive chambers (average of 1,5 magnification). After the large globular initial chamber, which indicates asexual reproduction, each chamber is slightly larger than the previous one. In the later stages the chambers develop lateral edges with a thickened margin, leading to a distinct triangular shape in cross section. Foram B on the other hand has a distinct trochospiral coil (like a snail), consisting of 16 chambers with a total volume of 91 x 106 µm³. The entire shell thickens with every successive chamber, so that the initial part of the test is embraced in a thick calcite crust. The chambers grow rapidly in size (average magnification of 2,24 ), which is typical for most planktic foraminifera. The globular shape aids in the buoyancy of the specimen for its planktic way of life

The upper Eocene-Oligocene carnivorous mammals from the Quercy Phosphorites (France) housed in Belgian collections

The Quercy Phosphorites Formation in France is world famous for its Eocene to Miocene faunas, especially those from the upper Eocene to lower Oligocene, the richest of all. The latter particularly helped to understand the ‘Grande Coupure’, a dramatic faunal turnover event that occurred in Europe during the Eocene-Oligocene transition. Fossils from the Quercy Phosphorites were excavated from the middle 19th century until the early 20th century in a series of sites and became subsequently dispersed over several research institutions, while often losing the temporal and geographical information in the process. In this contribution, we provide an overview and reassess the taxonomy of these barely known collections housed in three Belgian institutions: the Université de Liège, KU Leuven, and the Royal Belgian Institute of Natural Sciences. We focus our efforts on the carnivorous mammals (Hyaenodonta and Carnivoramorpha) and assess the stratigraphic intervals covered by each collection. These fossils are derived from upper Eocene (Priabonian), lower Oligocene (Rupelian), and upper Oligocene (Chattian) deposits in the Quercy area. The richness of the three collections (e.g., the presence of numerous postcranial elements in the Liège collection), the presence of types and figured specimens in the Leuven collection, and some identified localities in the RBINS collection make these collections of great interest for further studies on systematics and the evolution of mammals around the ‘Grande Coupure’

Molecular characterization and identification of proteins regulated by Hfq in Neisseria meningitidis

Hfq is a highly conserved pleiotropically acting prokaryotic RNA-binding protein involved in the post-transcriptional regulation of many stress-responsive genes by small RNAs. In this study, we show that Hfq of the strictly human pathogen Neisseria meningitidis is involved in the regulation of expression of components involved in general metabolic pathways, iron metabolism and virulence. A meningococcal hfq deletion strain (H44/76Δhfq) is impaired in growth in nutrient-rich media and does not grow at all in nutrient-limiting medium. The growth defect was complemented by expression of hfq in trans. Using proteomics, the expression of 28 proteins was found to be significantly affected upon deletion of hfq. Of these, 20 proteins are involved in general metabolism, among them seven iron-responsive genes. Two proteins (PilE, TspA) are involved in adherence to human cells, a step crucial for the onset of disease. One of the differentially expressed proteins, GdhA, was identified as an essential virulence factor for establishment of sepsis in an animal model, studied earlier. These results show that in N. meningitidis Hfq is involved in the regulation of a variety of components contributing to the survival and establishment of meningococcal disease

On the morphology of the astragalus and calcaneus of the amphicyonids (Carnivora, Mammalia) from the Paleogene of Europe: implications for the ecology of the European bear-dogs

peer reviewedThe Paleogene mammals of Europe are rarely known from partial or complete skeletons. As a result, their systematics and ecology are often solely based on dental characters and postcranial remains, when available, are usually neglected. This explains why the locomotion of mammals of the Eocene-Oligocene transition, the “Grande Coupure”, is poorly known. The aim of this study is to describe the tarsal bones (astragali and calcanei) and characterize the locomotion of amphicyonid carnivorans, one of the most abundant mammalian predator groups from the Phosphorites du Quercy (France) sites. The identification of taxa and the characterization of both posture and locomotion were carried out using four criteria: relative abundance (in comparison with dental data), morphology, size, and body mass. Seven morphotypes, four among astragali and three among calcanei, are identified as Amphicyonidae and show various postures: plantigrade, semi-digitigrade, and digitigrade. One morphotype of the astragalus and one of the calcaneus are identified as Cynodictis lacustris Gervais, 1852, which exhibits a digitigrade posture. The study of postcranial bones, such as tarsals, allows for a better understanding of the ecology of these animals and deserves more interest in future morphological and phylogenetic studies

Evolution of European carnivorous mammal assemblages through the Palaeogene

The rise of Carnivora (Mammalia: Laurasiatheria) is an important evolutionary event that changed the structure of terrestrial ecosystems, starting at the dawn of the Eocene, 56 Mya. This radiation has been mainly analysed in North America, leaving the evolution of carnivoran diversity in other regions of the globe poorly known. To tackle this issue, we review the evolution of terrestrial carnivorous mammal diversity (Mesonychidae, Oxyaenidae, Hyaenodonta and Carnivoramorpha) in Europe. We reveal four episodes of intense faunal turnovers that helped establish the dominance of carnivoramorphans over their main competitors. We also identify two periods of general endemism. The remaining time intervals are characterized by dispersals of new taxa from North America, Asia and Africa. The European Palaeogene carnivorous mammal fauna appears to have been almost constantly in a transient state, strongly influenced by dispersals. Many of the bioevents we highlight for European carnivorous mammals are probably best seen as ecosystem-wide responses to environmental changes. In contrast to the North American record, European hyaenodonts remain more diverse than the carnivoramorphans for the entire Eocene. The replacement of hyaenodonts by carnivoramorphans as the most diverse and dominant predators only occurred after the ‘Grande Coupure’ at 33 Mya, about 16 Myr later than in North America