‘Sangiovese’ and ‘Garganega’ are two key varieties of the Italian grapevine assortment evolution

Two synonymous cases have been found using a set of 11 SSR markers: ‘Garganega’ and ‘Grecanico dorato’; ‘Catarratto bianco comune’, ‘Catarratto bianco lucido’ and ‘Catarratto bianco extra lucido’. Molecular data at 36 SSR loci showed that ‘Sangiovese’ and ‘Garganega’ represent two key varieties in the Italian ampelographic assortment evolution, as they both have a first degree relationship with numerous wine varieties. ‘Sangiovese’ showed this link with ten varieties: ‘Foglia tonda’, ‘Frappato’, ‘Gaglioppo’, ‘Mantonicone’, ‘Morellino del Casentino’, ‘Morellino del Valdarno’, ‘Nerello mascalese’, ‘Susumaniello’, ‘Tuccanese di Turi’ and ‘Vernaccia nera del Valdarno’. Seven varieties resulted closely related to ‘Garganega’: ‘Trebbiano toscano’ alias ‘Ugni blanc’, ‘Albana’, ‘Empibotte’, ‘Malvasia bianca di Candia a sapore semplice’, ‘Marzemina bianca’, ‘Catarratto’ and ‘Greco del Pollino’. However, being ‘Sangiovese’ parents disputed and those of ‘Garganega’ still unknown, it was not possible to determine the univocal direction of the various crosses. Identification of the “missing” parents would allow these genealogical trees to be drawn up with greater precision.

Vino e Ambiente: sostenibilità e qualità primaria nel sottobacino Iudeo-Bucari (TP).

In questa raccolta di scritti, vengono riportati i risultati dell’attività di ricerca realizzata con la collaborazione della cantina UVAM e dell’Istituto Regionale Vino e Olio di Sicili

La risposta produttiva di alcuni vitigni nel sottobacino Iudeo - Bucari (TP).

Gli Autori dopo aver descritto le caratteristiche ambientali del sottobacino Iudeo Bucari e l’attività agricola prettamente legata alla vitivinicoltura, soffermano l’attenzione sulle potenzialità produttiva di questo territorio espressa da diversi vitigni. L'obiettivo è quello di trasferire su di una basecartografica il diverso comportamento produttivo dei vitigni più rappresentati nel sottobacino Iudeo - Bucari, con il fine ultimo di fornire un valido strumento agli agronomi liberi professionisti ed ai tecnici delle cantine al fine di indirizzare l’attività produttiva dei viticoltori e migliorare la gestione dei suoli del territorio

Moscato Cerletti, a rediscovered aromatic cultivar with oenological potential in warm and dry areas

Baron Antonio Mendola was devoted to the study of grapevine, applying ampelography and dabbling in crosses between cultivars in order to select new ones, of which Moscato Cerletti, obtained in 1869, was the most interesting. Grillo, one of the most important white cultivars in Sicily, was ascertained to be an offspring of Catarratto Comune and Zibibbo, the same parents which Mendola claimed he used to obtain Moscato Cerletti. Thus the hypothesis of synonymy between Moscato Cerletti and Grillo or the same parentage for both sets of parents needs to be verified. In the present study, historical documents were consulted and genetic analyses and ampelographic, agronomic and qualitative characterisation carried out to determine the distinctiveness of each cultivars. These were also compared with Catarratto Comune and Zibibbo in order to establish the Moscato Cerletti pedigree. Due to their different SSR profiles, Grillo and Moscato Cerletti were confirmed as two distinct cultivars; they also differed in ripening times and sugar storage ability, as well as in the aromatic grape produced by Moscato Cerletti only. The trio genotype genetic analysis confirmed that Zibibbo is a parent of Moscato Cerletti (justifying the aromatic grape), whilst the SSR profiles did not show Catarratto Comune to be a second parent. Moscato Cerletti was found to have oenological potential in the production of sparkling muscat wines due to its ability to adapt to a changing climate in warm and dry environments and in different winegrowing regions

Le prove di vinificazione della cultivar grillo su tre vigneti sperimentali site nel sottobacino Iudeo - Bucari (TP).

Gli Autori, dopo aver descrittole caratteristiche ambientali del sottobacino Iudeo Bucari e l’attività agricola prevalentemente legataalla vitivinicoltura, descrivono alcune prove di vinificazione di uve Grillo provenienti da tre vignesite nel sottobacino Iudeo – Bucari gestite in modo ordinario, delle quali si è monitorata la maturazione delle uve. L'obiettivo è quello di caratterizzare il prodotto vino ottenuto attraverso una tecnica di vinificazione uniforme ed ordinaria nella zona, ma che mira a mettere in evidenza la diversa qualità tecnologica delle uve utilizzate espressione dicontesti eco-pedologici diversi

Prevention of methamphetamine-induced microglial cell death by TNF-α and IL-6 through activation of the JAK-STAT pathway

<p><b>Abstract</b></p> <p><b>Background</b></p> <p>It is well known that methamphetamine (METH) is neurotoxic and recent studies have suggested the involvement of neuroinflammatory processes in brain dysfunction induced by misuse of this drug. Indeed, glial cells seem to be activated in response to METH, but its effects on microglial cells are not fully understood. Moreover, it has been shown that cytokines, which are normally released by activated microglia, may have a dual role in response to brain injury. This led us to study the toxic effect of METH on microglial cells by looking to cell death and alterations of tumor necrosis factor-alpha (TNF-α) and interleukine-6 (IL-6) systems, as well as the role played by these cytokines.</p> <p><b>Methods</b></p> <p>We used the N9 microglial cell line, and cell death and proliferation were evaluated by terminal deoxynucleotidyl transferase dUTP nick end labeling assay and incorporation of bromodeoxyuridine, respectively. The TNF-α and IL-6 content was quantified by enzyme-linked immunosorbent assay, and changes in TNF receptor 1, IL-6 receptor-alpha, Bax and Bcl-2 protein levels by western blotting. Immunocytochemistry analysis was also performed to evaluate alterations in microglial morphology and in the protein expression of phospho-signal transducer and activator of transcription 3 (pSTAT3).</p> <p><b>Results</b></p> <p>METH induced microglial cell death in a concentration-dependent manner (EC₅₀ = 1 mM), and also led to significant morphological changes and decreased cell proliferation. Additionally, this drug increased TNF-α extracellular and intracellular levels, as well as its receptor protein levels at 1 h, whereas IL-6 and its receptor levels were increased at 24 h post-exposure. However, the endogenous proinflammatory cytokines did not contribute to METH-induced microglial cell death. On the other hand, exogenous low concentrations of TNF-α or IL-6 had a protective effect. Interestingly, we also verified that the anti-apoptotic role of TNF-α was mediated by activation of IL-6 signaling, specifically the janus kinase (JAK)-STAT3 pathway, which in turn induced down-regulation of the Bax/Bcl-2 ratio.</p> <p><b>Conclusions</b></p> <p>These findings show that TNF-α and IL-6 have a protective role against METH-induced microglial cell death via the IL-6 receptor, specifically through activation of the JAK-STAT3 pathway, with consequent changes in pro- and anti-apoptotic proteins.</p

Adherence to antibiotic treatment guidelines and outcomes in the hospitalized elderly with different types of pneumonia

Background: Few studies evaluated the clinical outcomes of Community Acquired Pneumonia (CAP), Hospital-Acquired Pneumonia (HAP) and Health Care-Associated Pneumonia (HCAP) in relation to the adherence of antibiotic treatment to the guidelines of the Infectious Diseases Society of America (IDSA) and the American Thoracic Society (ATS) in hospitalized elderly people (65 years or older). Methods: Data were obtained from REPOSI, a prospective registry held in 87 Italian internal medicine and geriatric wards. Patients with a diagnosis of pneumonia (ICD-9 480-487) or prescribed with an antibiotic for pneumonia as indication were selected. The empirical antibiotic regimen was defined to be adherent to guidelines if concordant with the treatment regimens recommended by IDSA/ATS for CAP, HAP, and HCAP. Outcomes were assessed by logistic regression models. Results: A diagnosis of pneumonia was made in 317 patients. Only 38.8% of them received an empirical antibiotic regimen that was adherent to guidelines. However, no significant association was found between adherence to guidelines and outcomes. Having HAP, older age, and higher CIRS severity index were the main factors associated with in-hospital mortality. Conclusions: The adherence to antibiotic treatment guidelines was poor, particularly for HAP and HCAP, suggesting the need for more adherence to the optimal management of antibiotics in the elderly with pneumonia